Supplemental Data. Yang et al. (2015). Plant Cell 10.1105/tpc.15.00091

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Supplemental Figure 1. The lateral root number in WT, brm-1, brm-3, and

brm-5 roots at 10 DAG.

Supplemental Figure 2. The expression of CycB1;1 and CycB1;3 in 3 DAG WT,

brm-3 and brm-5 roots. Bar=50 μm.

Supplemental Data. Yang et al. (2015). Plant Cell 10.1105/tpc.15.00091

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Supplemental Figure 3. Cellular organization of WT (Col-0), brm-3, brm-5 and

brm-1 root tips at 3 DAG using propidium iodide staining (A) and the length of

4th columella cell of the WT (Col-0), brm-3 and brm-5 root tips at 3 DAG (B).

The QC (white triangle) and columella cell areas (white asterisk) are indicated.

Bars = 20 μm. The 4th columella cell of brm-1 root tips could not be identified.

Data shown are means ± SD (n =20).

Supplemental Data. Yang et al. (2015). Plant Cell 10.1105/tpc.15.00091

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Supplemental Figure 4. The expression of PIN2 in WT (Col-0) and brm-3 root

tips at 3 DAG (A) and the expression of root development related genes in WT

(Col-0) and brm-1 5-day-old seedlings. Bars = 20 μm.

Supplemental Data. Yang et al. (2015). Plant Cell 10.1105/tpc.15.00091

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Supplemental Figure 5. The expression pattern of SHR and SCR in WT and

brm-3 (A, C) and the phenotype of shr-1 brm-3 (B), scr-1 brm-3 (D), shr-1

brm-1 (E) and scr-1 brm-1 (F) double mutants. Bars = 20 μm (A, C) and 1 cm (B,

D, E, F).

Supplemental Data. Yang et al. (2015). Plant Cell 10.1105/tpc.15.00091

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Supplemental Figure 6. The phenotype of plt1-4 plt2-2 brm-3 (A) and plt1-4

plt2-2 brm-1 (B) triple mutants at 7 DAG. Bars = 1 cm.

Supplemental Figure 7. The phenotype of Col-0 and BRMpro:BRM:GFP/brm-1

Supplemental Data. Yang et al. (2015). Plant Cell 10.1105/tpc.15.00091

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seedlings at 10 DAG . Bars = 0.5 cm.

Supplemental Figure 8. ChIP-qPCR analysis of BRM targeting to PIN3, PIN4

and PIN7 (A) and H3K27me3 levels of PIN3, PIN4 and PIN7 loci in brm-3

mutant roots (B). Data are mean values ± standard deviation of three

replicates. Similar results were obtained for at least two additional independent

experiments. Asterisks denote Student’s t- test significant difference between

Col-0 and BRMpro: BRM:GFP/Col-0 roots (p<0.05).

Supplemental Data. Yang et al. (2015). Plant Cell 10.1105/tpc.15.00091

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Supplemental Figure 9. BRM does not target to PLT1 and PLT2 directly.

Supplemental Figure 10. The normal expression of QC25 and QC46 in WT

Supplemental Data. Yang et al. (2015). Plant Cell 10.1105/tpc.15.00091

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(Col-0) and brm-3 root tips at 3 DAG. Bars = 25 μm.

Supplemental Table 1. Primers used for RT-qPCR

Primer

name

Forward Reverse

PLT1 AGTTCGTGGCTGCCATTAGAAGG ATCTTCCATGTTGGTGATGCCTTG

PLT2 GAAGATGGCAAGCAAGGATCGG GCTTCTTCCTCCGTGCTGAATG

PIN1

GGCATGGCTATGTTCAGTCTTGG

G

ACGGCAGGTCCAACGACAAATC

PIN2

TCACGACAACCTCGCTACTAAAG

C

TGCCCATGTAAGGTGACTTTCCC

PIN3 AAGGCGGAAGATCTGACCAAGG TGCTGGATGAGCTACAGCTTTG

PIN4 ACAACGTGGCAACGGAACAATC GCCGATATCATCACCACCACTC

PIN7 CGTGTGGCCATTGTTCAAGCTG

CCCTGTACTCAAGATTGCGGGAT

G

CYB1;1 CACTATTTGGCTGAGTTAGG ATCTTGCTGCGTAGATTGC

CYB1;3 TACTCGTAACTTTCGTGCTCAG GAACCGCTCTTACAACTTCTTG

UBQ10 TCGACCCTTCACTTGGTGTTGC

GGGTGATGGTCTTTCCGGTCAAA

G

Supplemental Table 2. Primers used for ChIP-qPCR

Primer

name

Forward Reverse

Length of

amplicon

Supplemental Data. Yang et al. (2015). Plant Cell 10.1105/tpc.15.00091

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PLT1-P1 gaggacgtgagttcccataatc gcaaattaattatcactcacaatg 132 bp

PLT1-P2 AGGAGAGGTTCCAAAAGTGGCC ATTGGACGCTAGGCATCAAG 131 bp

PLT1-P3 ttccacttaattaggttttgtc ttatacgattcgatgcactcac 150 bp

PLT1-P4 TGGCTGCCATTAGAAGgttt GATACATCGAAGCGCCTCTC 140 bp

PLT1-P5 GGCTCCACCTCATCAAGACT AAGAGGAGGAATCGTGAGCA 133 bp

PLT1-P6 tcaatttagagggggccttt cccataaaaccctaaccctca 120 bp

PLT2-P1 taaacgagggacgtgagtt cgcttccaactagaaaaagtc 130 bp

PLT2-P2 ccaagaaaagggaaATGAA GGTTTTGAAAAGGGTTGTCG 148 bp

PLT2-P3 CAGAAGTCGCCACTGTGAAA CCAAAAGTTTCCAAAGTCCGT 124 bp

PLT2-P4 tcggactatgcactcagGTG tcatgaaaatttgaaataacttacCG 130 bp

PLT2-P5 AACGGCTCAAAGAAGCTCAA AGAGCTGGAAGCAACAGAGC 122 bp

PLT2-P6 acaagtattaggatcggctgga ccctcgaggactctgataacc 120 bp

PIN1-P1 gggataaattgatcaaccgaat ggtggagctttggggatagt 140 bp

PIN1-P2 ttccctcttcaccacttctctc ACGGAACCATAGCCGTCATA 128 bp

PIN1-P3 GAACCCAGGGATGTTTTCG GCACTTGAGCTCCACACAAA 135 bp

PIN1-P4 CCTCCATGTTGCCATTATCC AAGCTGCctgtttgttgtca 125 bp

PIN1-P5 tgccctttttgttgaaaacc aaggtaaaacttgctgagctcct 127 bp

PIN2-P1 ccaatcttccttatcccaaga ggtccataatttttcgaagtgc 137 bp

PIN2-P2 tctctcgccggaaaaagtaa GTGTGAATATCCCCCACCAC 132 bp

PIN2-P3 TCTCACCGGCGTAGAGATTT GCCGCCGTAGCTATTAGTGT 148 bp

PIN2-P4 ttaaatagatataagaatcgatt aattattattattttgttaaaaga 146 bp

PIN2-P5 ttgcaaataaaaggcgatacg attaatgcgacattgcatgg 128 bp

Supplemental Data. Yang et al. (2015). Plant Cell 10.1105/tpc.15.00091

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PIN3-P1 aaaatcaagcacatgaatgtcac tcgactgattgaaacggaca 122 bp

PIN3-P2 cactgctcccaagtcctctc AGGATCATGGCCACGTAGAG 147 bp

PIN3-P3 AGGTCCAACTCCTCGACCTT GACGCTTTTATTGGCGGTAG 154 bp

PIN3-P4 TGGACTTATTTGGGCTCTCG GATTTTGGGCATTGCCACGTG 135 bp

PIN3-P5 acgcagataaggaggagcaa cccgaacctaatcaaagaaca 153 bp

PIN4-P1 gagttttctggagggacgtg cgtagtattcgccaaagttcc 129 bp

PIN4-P2 tcctcttcaccgaatccttg cggtgggttttggagtttag 144 bp

PIN4-P3 ctgagaaaaccaaaaatcatgaatac tcctcttcaccgaatccttg 120 bp

PIN4-P4 tgaccctctgacccagacat ggatttgagaaaattgacagca 127 bp

PIN4-P5 TCTTGGCCTTTGAattggtc cttccactctttcccacgaa 121 bp

PIN7-P1 aagttttggaccggcattta caatgaattttgtgaatccttga 140 bp

PIN7-P2 tgttaaaagcttccgtcatca AGGACGGTGTAGAGGTCGTG 140 bp

PIN7-P3 CATACTCAAAATGGTGAAAAC ATAGTCCCCGTTCATCGGAC 125 bp

PIN7-P4 ggtgggatgacatagctcgt aacaagtgctgaatgatgcaa 153 bp

PIN7-P5 tctgcttggctgaagaggat ggatcctcaaatcaaatcttgg 125 bp

TA3 ctgcgtggaagtctgtcaaa ctatgccacagggcagttt 106bp

TUB2

ACAAACACAGAGAGGAGTGAGC

A

ACGCATCTTCGGTTGGATGAG

TG

150 bp