Zea mays Brachypodium distachyon Oryza sativa - Plant Cell · Supplemental Figure 1. SERF1 protein...

Supplemental Figure 1. SERF1 protein sequence. Sequence alignment of SERF1

(Os05g34730) and its homologous proteins from Zea mays (GRMZM2g307),

Sorghum bicolor (Sb03g04206), Brachypodium distachyon (Bradi2g572), Arabidopsis

thaliana (AT1G71520, AT1G22810) and Oryza sativa (Os01g66270). SERF1 shares

high sequence similarity regarding the AP2 binding domain consisting of three β-

sheets (β1-β3) and one α-helix. SERF1 contains two conserved helical structures (H1

and H2). The SERF1 protein has five conserved Ser (S) residues and one Thr (T)

residue (see blue stars for both) of which Ser105 forms a potential MAPK docking site

with Pro106 (indicated by a red star; Cohen, 1997).

Supplemental Data. Schmidtet al. (2013). Plant Cell 10.1105/tpc.113.113068

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Supplemental Figure 2. Overexpression of SERF1 Decreases Leaf Temperature under Short-term Salt Stress. (A) Leaf temperature of salt-stressed serf1 and wild-

type plants (200 mM NaCl); n = 10. (B) Thermographic images used to calculate the

temperature kinetics. Each picture shows four leaves of which the upper two are wild-

type and the bottom ones are serf1 leaves. Shown are four time points including 0

min (upper left), 2 min (lower left), 10 min (upper right), and 30 min (lower right). The

color temperature scale (°C) is depicted on the right. (C) Leaf temperature of salt-

stressed SERF1 knock-down (KD) and EV plants (200 mM NaCl); n = 6. (D) Leaf

temperature of salt-stressed SERF1 overexpression (OE) and EV plants (200 mM

NaCl); n = 6. Leaf temperature under stress (Tstress) was normalized to the

temperature measured under normal conditions (Tcontrol). Data represent means ± SE.

An asterisk (*) indicates a significant difference (p ≤ 0.05) to leaf temperature of salt-

stressed wild-type or EV plants as calculated by Student's t-test. EV, empty vector.

WT, wild type.


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Supplemental Figure 3. Loss and Knock-down of SERF1 Lead to Salt Sensitivity by Rapid Decline of Photosynthesis Under Salt Stress. Time course

of transpiration rate (TR), stomatal conductance (SC) and leaf net CO2 assimilation

rate (CAR) of 3-week-old serf1, SERF1 knock-down (KD 4-1), wild-type and EV

plants which were exposed to salt stress (50 mM NaCl) for seven days. Data

represent means SE (n=10) given as % of the corresponding non-stressed

genotype, and an asterisk (*) indicates a significant difference between serf1 and

wild-type, or KD 4-1 and EV, respectively, under salt stress (p 0.05). WT, wild type;

EV, empty vector.


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Supplemental Figure 4. SERF1 Affects Oxidative Stress Tolerance and Redox Homeostasis. Levels of chlorophyll a (chl a) and chlorophyll b (chl b) of leaf

segments of 14-day-old serf1 (A), SERF1 knock-down (KD) (B) and SERF1

overexpression (OE) plants (C) exposed to 5 µM methyl viologen (MV) or 10 mM

H2O2 for 36 h. Levels of chlorophyll a and b are expressed relative to mock-treated

plants. Data in (A) to (C) represent means SD from three independent biological

replicates. An asterisk (*) indicates a significantly stronger/weaker reduction of

pigments (p 0.05) in stressed serf1, SERF1 knock-down (KD) and overexpression

(OE) leaves as compared to stressed wild-type and EV leaves, respectively. Content

of (D) pyridine nucleotides, (E) GSH, and (F) ascorbate in serf1 and wild-type roots

under control conditions and after 30 min of salt stress (100 mM NaCl). Content of

(G) pyridine nucleotides, (H) reduced GSH, and (I) ascorbate in SERF1 knock-down


4

(KD) and EV roots under control conditions and after 30 min of salt stress (100 mM

NaCl). Data in (D) to (I) represent means SE from six independent biological

replicates and an asterisk (*) indicates a significant difference (p 0.05) to wild-type

and EV roots, respectively. DAB staining of (J) wild-type and serf1 roots before (left)

and after (right) 30 min of salt stress (100 mM NaCl) and (K) wild-type and serf1

leaves under control conditions (upper leaf) and after 30 min of salt stress (100 mM

NaCl; lower leaf). GSH, glutathione; WT, wild type; EV, empty vector; FW, fresh

weight.


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Supplemental Figure 5. Identification of Early Salt-responsive Marker Genes. (A) Venn diagram representation of shared/unique salt-responsive genes in roots

after 5 min, 30 min, 3 h and 24 h of stress treatment (100 mM NaCl). For example, of

the 190 identified genes, 16 respond only after 30 min, while 14 genes overlap

between 30 min, 3 h and 24 h. (B) The number of genes responding to salt stress

increases with time. (C) Expression pattern of the 70 genes selected for the

characterization of the response of serf1 mutant to salt stress.


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Supplemental Figure 6. SERF1 Affects Osmotic Homeostasis and Ion Detoxification/Compartmentalization Under Short-term Salt Stress in Roots. Transcript levels of genes encoding channels (A), transporters (B), ATPases (C) and

aquaporins (D) were measured in roots of wild-type and serf1 plants subjected to salt

stress (100 mM NaCl) for 30 min or 3 h; expression values are normalized to ACTIN.

Data represent mean ΔΔCT SE from three independent biological replicates, and

an asterisk (*) indicates a significant difference between stressed and mock-treated

plants (p 0.05). Heat maps indicate the response of each gene to 5 mM H2O2 (H), 5

µM ABA (A) or 100 mM mannitol (M) after 30 min or 3 h of treatment in wild-type

roots. Values are presented as log2FC relative to mock-treated wild-type roots. n = 3.

SKOR* and SKOR** are encoded by Os04g36740 and Os06g14030, respectively,

and Os06g46670, Os07g01310, Os09g26160 and Os09g31160 code for GLRs.


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CaCA* and CaCA** are encoded by Os11g01580 and Os12g42910, respectively.

FC, fold change; WT, wild type; GLR, Glutamate-like receptor; CaCA Calcium Cation

Antiporter.


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Supplemental Figure 7. SERF1 Affects the Expression of genes involved in Calcium-dependent Signalling. Transcript levels of calcium-dependent signalling

genes were determined in roots of wild-type and serf1 plants subjected to salt stress

(100 mM NaCl) for 30 min or 3 h; expression values are normalized to ACTIN. Data

represent mean ΔΔCT SE from three independent biological replicates, and an

asterisk (*) indicates a significant difference between stressed and mock-treated

plants (p 0.05). The heat map presents the response of each gene to 5 mM H2O2

(H), 5 µM ABA (A) or 100 mM mannitol (M) after 30 min or 3 h of treatment in wild-

type roots. Values presented are given as log2FC relative to mock-treated wild-type

roots. n = 3. FC, fold change; WT, wild type.


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Supplemental Table 1. Early Salt-Responsive Genes Identified. Data represent means

(∆∆CT) between control and salt-treated plants from three independent biological replicates,

statistically significant differences were determined by the Student's t-test. Bold font indicates

a significant up- or down-regulation (∆∆CT ≥ +1 or ≤ -1 with p ≤ 0.05) relative to the

expression in mock-treated roots. Genes used for expression profiling of serf1, SERF1

knock-down and overexpression plants under salt stress are marked in green.

log2FC Locus Description 5 min 30 min 3 h 24 h Os01g70660 HAK6 -1.29 -1.50 -3.65 -0.88

Os02g55890 OVP4 -1.19 0.27 -0.14 1.65 Os03g55560 MAP3K15 -1.00 0.28 1.01 1.14 Os05g39090 CPK16 -1.37 -0.49 0.34 1.09 Os06g08930 GLR2.8 -1.50 0.27 -0.83 -0.06

Os07g01310 GLR -1.23 -1.24 -0.30 1.02 Os08g36340 HAK4 -1.31 -1.08 -3.01 -0.48

Os09g26160 GLR -1.33 0.23 0.04 1.81 Os10g35050 aquaporin protein -1.00 1.04 1.17 2.27 Os12g41090 CIPK4 -1.05 -0.80 -0.16 0.87

Os05g46760 MAP3K19 1.01 2.37 1.41 1.45 Os06g09120 glutamate receptor 2.8 precursor 1.00 0.64 1.01 1.65 Os06g40370 CIPK24 - SOS2 1.11 1.75 1.22 1.37 Os08g05600 aquaporin protein 1.87 -0.08 -0.37 0.49

Os08g34240 CIPK6 1.26 0.20 0.71 0.17

Os09g25090 CAMK_KIN1/SNF1/Nim1_like.34 1.03 1.63 0.43 1.20 Os09g26000 glutamate receptor 1.24 1.23 0.03 1.99 Os09g30446 CPA1 1.46 1.06 1.49 1.01

Os02g58520 CPK6 -0.79 -1.43 0.35 0.33

Os05g35410 AKT2/3 -0.79 -1.00 -0.32 -0.73

Os01g13120 TIP4-3 0.47 -1.05 -1.18 -2.05 Os05g26820 CIPK18 -0.10 -1.09 0.36 -0.03

Os11g01580 CacA family 0.36 -1.00 -0.06 -0.55

Os06g46670 GLR 0.01 -1.02 0.03 -0.01

Os02g07830 HKT6 -0.34 -1.11 -0.73 -0.06

Os04g51830 HKT7 0.51 -1.50 0.23 0.66

Os02g03410 CPK4 0.16 1.97 1.00 1.11 Os02g14840 KAT1 0.52 1.23 2.24 2.30 Os03g48310 AHA1 0.32 1.23 1.63 1.77 Os01g70940 HAK2 0.59 1.18 1.49 1.66 Os02g41860 PIP-2-2 -0.33 1.01 0.31 -0.02

Os03g12390 MAP2K6 0.35 2.07 1.04 1.46 Os05g46750 MAP3K18 -0.01 2.49 1.26 1.13

Os12g42910 CacA family -0.18 1.30 1.48 0.66

Os01g50410 MAP3K6 0.32 2.68 1.02 1.33 Os03g55100 CNGC2 -0.19 1.00 -0.26 -0.02

Os10g04010 MAP3K23 2.37 1.92 -0.35 -2.41

Os06g08880 GLR2.7 0.29 1.05 1.61 1.51 Os06g48810 HKT1 0.92 1.67 0.57 -0.24

Os06g37180 ATP synthase -0.11 1.27 0.39 0.88

Os02g46090 CAMK_CAMK_like.15 0.42 1.22 0.69 0.85

Os01g61780 vacuolar ATP synthase 98 kDa subunit 0.09 1.00 0.58 1.05 Os07g47350 potassium transporter -0.08 1.29 1.03 1.80 Os11g29750 plasma membrane ATPase 0.39 1.11 1.23 1.47 Os11g29490 plasma membrane ATPase 0.40 1.03 1.02 1.33


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Os05g04550 CAMK_KIN1/SNF1/Nim1_like.3 -0.13 1.41 0.71 1.05 Os03g37930 potassium transporter, , -0.19 1.04 0.52 0.81

Os07g44290 CAMK_KIN1/SNF1/Nim1_like.29 0.04 1.36 1.03 1.15 Os02g14980 calcium-binding EF hand family protein 0.73 1.45 1.11 0.73

Os03g45370 sodium/calcium exchanger protein 0.24 1.15 0.57 1.59 Os02g54600 STE_MEK_ste7_MAP2K.5 0.26 1.17 0.70 1.46 Os04g56530 STE_MEKK_ste11_MAP3K.1 0.02 1.00 0.37 1.08 Os03g03510 CAMK_KIN1/SNF1/Nim1_like.15 0.34 1.50 0.60 1.14 Os07g05620 CAMK_KIN1/SNF1/Nim1_like.28 0.16 1.47 1.28 1.18 Os02g35010 STE_MEKK_ste11_MAP3K.9 0.19 1.05 0.71 1.42 Os01g50400 STE_MEKK_ste11_MAP3K.5 0.03 1.53 0.17 1.38 Os02g21700 STE_MEKK_ste11_MAP3K.8 0.00 1.06 0.44 0.76

Os05g42250 cyclic nucleotide-gated ion channel 2 0.01 1.33 1.35 1.90 Os06g10580 cyclic nucleotide-gated ion channel 0.15 1.18 0.74 1.02 Os06g09190 mitogen-activated protein kinase kinase 5 -0.38 1.07 0.74 1.60 Os06g21360 transporter, monovalent cation:proton

antiporter-2 family -0.39 1.99 1.13 1.76

Os03g17700 CGMC_MAPKCGMC_2_ERK.2 kinase 0.19 1.32 0.58 1.17 Os04g36740 SKOR 0.05 -0.57 -1.58 0.17

Os06g14030 SKOR 0.37 -0.86 -1.01 0.45

Os01g73130 VHA-c4 0.98 0.05 -1.72 -0.37

Os05g26940 CIPK22 0.03 0.34 -1.05 -0.32

Os02g18930 CBL8 0.28 -0.51 -1.03 -0.17

Os01g20160 HKT8 0.40 -0.33 -2.66 -0.85

Os01g45990 AKT1 -0.44 -0.39 -2.16 -0.84

Os05g51530 VHA-C -0.08 0.46 1.03 0.09

Os06g43030 Protein kinase -0.48 0.94 3.10 3.09 Os12g30150 CAMK_CAMK_like.47 -1.69 -0.05 1.08 0.38

Os06g15910 HAK24 -0.04 -0.21 1.14 0.23

Os05g50560 MPK9 0.03 -0.06 1.01 0.29

Os11g17080 MPK15 -0.23 0.22 1.02 0.25

Os02g13870 NIP1-1 -0.05 0.52 2.34 2.90 Os01g51420 CBL10 -0.56 -0.89 1.15 1.17 Os08g42750 CAMK_CAMK_like.37 0.52 0.90 1.33 1.98 Os01g02190 aquaporin protein 0.54 0.47 1.37 0.42

Os07g01810 potassium channel protein 0.45 -0.85 1.01 1.27 Os09g36930 aquaporin protein -0.43 0.64 1.38 3.24 Os10g06800 cyclin-dependent kinase G-2 0.61 -0.35 1.09 3.51 Os06g48590 CGMC_MAPKCMGC_2_SLT2y_ERK.2 0.33 0.93 1.00 1.46 Os04g16450 aquaporin protein -0.31 0.46 1.05 1.50 Os01g10890 CAMK_KIN1/SNF1/Nim1_like.8 0.52 0.67 1.01 1.49 Os06g08310 plasma membrane ATPase 0.10 0.41 1.25 1.63 Os02g44080 TIP2-1 -0.16 0.24 -0.02 -1.18 Os02g57720 PIP1-3 -0.05 0.53 0.44 -1.28 Os04g44060 PIP2-3 -0.94 -0.28 -0.02 -1.05 Os07g26640 aquaporin 0.10 -0.01 -0.57 -1.00 Os07g26630 PIP2-4 -0.03 -0.28 -0.52 -1.03 Os07g26690 PIP2-1 0.00 0.11 0.01 -1.01 Os01g50120 MAPK3K6 -0.47 -0.18 0.25 1.04 Os07g44710 CPK30 -0.47 -0.05 -0.18 1.04 Os09g38960 HAK18 -0.53 -0.35 0.31 1.00 Os06g45940 HAK13 -0.98 -0.47 0.12 2.37 Os01g47530 MPK8 -0.56 0.39 -0.26 1.46 Os01g13130 TIP4-2 0.65 -0.11 0.34 1.54 Os04g44570 TIP3-2 -0.17 0.63 0.89 4.51 Os07g48760 CIPK3 0.25 -0.33 0.09 1.08 Os06g12310 NIP2-2 -0.81 -0.13 0.57 1.16


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Os01g50370 MAP3k4 0.08 0.58 -0.68 1.09 Os07g43900 protien kinase -0.27 -0.13 -0.05 1.03 Os04g49570 GLR3.1 -0.23 -0.40 -0.26 1.41 Os03g15570 MAP3K12 -0.37 -0.18 -0.31 1.29 Os05g51610 CAX1b -0.62 -0.36 0.28 1.01 Os03g17310 ECA1 -0.66 -0.12 0.06 1.25 Os05g41580 ACA7 0.03 -0.23 -0.45 1.08 Os09g31160 GLR -0.34 -0.21 0.09 1.04 Os01g46980 vacuolar ATP synthase subunit E 0.36 0.07 0.18 1.04 Os01g59360 CAMK_CAMK_like.10 0.59 0.62 0.25 1.77 Os03g59390 CAMK_CAMK_like.24 0.03 0.69 0.06 1.25 Os04g47300 CAMK_CAMK_like.26 0.44 0.71 0.42 1.21 Os01g11250 potassium channel KAT1 -1.07 0.47 0.26 1.29 Os01g52070 potassium channel AKT1 2.27 0.40 -0.89 1.48 Os02g54510 protein kinase domain containing protein 0.09 0.70 0.52 1.38 Os02g32610 protein kinase domain containing protein -0.16 0.47 0.18 1.01 Os03g06410 protein kinase domain containing protein 0.19 0.94 0.95 1.50 Os06g08280 protein kinase domain containing protein -0.21 0.31 0.56 2.07 Os09g12790 potassium channel protein -0.61 0.19 0.31 1.38 Os05g50810 CAMK_CAMK_like.5 0.23 0.86 0.48 1.04 Os11g07040 CAMK_CAMK_like.43 0.15 0.41 0.42 1.10 Os05g50190 protein kinase domain containing protein 0.30 0.64 0.33 1.71 Os08g12750 serine/threonine-protein kinase HT1 0.44 0.73 0.57 1.43 Os03g43760 protein kinase domain containing protein 0.41 0.53 0.65 1.25 Os03g60150 protein kinase domain containing protein 0.22 0.80 0.47 1.04 Os01g70490 potassium transporter 0.10 0.36 0.56 2.11 Os02g49760 potassium transporter 0.14 0.57 0.51 1.01 Os03g21890 potassium transporter -0.59 -0.03 0.43 1.09 Os11g04170 CAMK_CAMK_like.42 -0.04 0.29 0.20 1.51 Os03g01120 E1-E2 ATPase domain containing protein -0.52 0.40 0.10 1.42 Os12g03970 CAMK_CAMK_like.44 -0.06 0.22 0.38 1.04 Os12g12860 CAMK_CAMK_like.46 0.05 0.36 0.64 1.39 Os06g43660 inorganic H+ pyrophosphatase 0.00 0.70 0.64 1.16 Os10g38950 CGMC_MAPKCMGC_2_ERK.14 0.18 1.00 0.84 1.22 Os01g10870 CAMK_KIN1/SNF1/Nim1_like.7 -0.06 0.48 0.75 1.03 Os07g48130 potassium transporter -0.13 0.74 0.80 1.46 Os03g22050 CAMK_KIN1/SNF1/Nim1_like.16 0.37 0.83 0.65 1.57 Os05g11790 CAMK_KIN1/SNF1/Nim1_like.19 0.78 0.35 -0.22 1.39 Os01g23580 inorganic H+ pyrophosphatase -0.03 0.69 0.70 1.78 Os01g43910 CGMC_MAPKCMGC_2.4 -0.25 0.41 0.74 1.29 Os02g04230 CGMC_MAPKCMGC_2.7 0.13 0.19 0.99 1.63 Os01g45620 CGMC_MAPKCMGC_2.5 0.25 0.88 0.29 1.23 Os05g43840 CAMK_KIN1/SNF1/Nim1_like.25 0.21 0.18 0.31 1.29 Os09g27580 potassium transporter 0.11 0.80 0.98 1.69 Os02g04630 sodium/calcium exchanger protein -0.36 0.74 0.44 1.25 Os03g08230 sodium/calcium exchanger protein -0.05 0.78 0.55 1.03 Os01g32660 STE_MEK_ste7_MAP2K.2 0.09 0.91 0.65 1.37 Os02g44642 STE_MEKK_ste11_MAP3K.10 0.21 0.51 0.42 1.16 Os11g05070 sodium/calcium exchanger protein 0.34 0.93 0.62 1.57 Os01g55440 CAMK_KIN1/SNF1/Nim1_like.1 0.65 1.78 0.96 2.52 Os04g55080 cyclic nucleotide-gated ion channel 0.14 0.71 0.64 1.08 Os07g48760 CAMK_KIN1/SNF1/Nim1_like.32 0.25 -0.33 0.09 1.08 Os06g08850 cyclic nucleotide-gated ion channel 14 -0.22 0.15 0.23 1.10 Os06g33570 cyclic nucleotide-gated ion channel 1 0.31 0.35 0.91 1.50 Os09g38580 cyclic nucleotide-gated ion channel -0.23 0.65 0.48 1.15 Os05g14240 aquaporin protein -0.11 0.86 -0.24 1.48 Os05g11560 aquaporin protein -0.25 0.36 0.58 1.61


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Os06g35930 aquaporin protein 0.25 0.53 -0.51 1.67 Os02g12810 protein kinase domain containing protein -0.21 0.91 0.49 1.27 Os02g38080 protein kinase domain containing protein 0.15 0.79 0.71 1.23 Os06g50030 CAMK_CAMK_like.30 0.08 0.79 0.37 1.13 Os02g53340 cyclic nucleotide-gated ion channel -0.13 0.18 0.05 1.01 Os01g39770 calcineurin B 0.35 0.29 0.56 1.02 Os02g54760 cyclic nucleotide-gated ion channel 14 0.19 0.40 0.32 1.12 Os06g33610 cyclic nucleotide-gated ion channel 1 0.29 0.45 0.58 1.34 Os01g48680 two pore calcium channel protein 1 0.22 0.84 0.03 1.01 Os10g36924 aquaporin protein 0.01 0.57 0.14 1.00 Os02g02540 glutamate receptor 0.18 0.72 0.71 1.00 Os11g10100 STE_MEKK_ste11_MAP3K.3 0.18 0.52 0.70 1.79 Os06g08910 glutamate receptor 2.8 precursor 0.72 0.79 0.73 1.78 Os03g49640 STE_MEKK_ste11_MAP3K.14 0.33 0.88 0.50 1.36 Os06g13730 glutamate receptor precursor 0.82 -0.13 -0.21 1.42 Os05g45810 CBL4/SOS3 0.52 0.15 0.49 1.04 Os10g41510 calcineurin B 0.48 0.97 0.91 1.80 Os04g55940 sodium/calcium exchanger protein, , 0.21 0.52 0.32 1.03 Os02g08010 LTPL43 - Protease inhibitor/seed storage/LTP

family protein precursor 0.24 0.21 -0.21 1.25

Os11g42790 transporter, monovalent cation:proton antiporter-2 family

0.14 0.98 0.71 1.50

Os04g51610 calcium-transporting ATPase, plasma membrane-type

0.17 0.66 0.81 1.30

Os06g09090 glutamate receptor 2.8 precursor 0.48 0.59 0.89 1.37 Os10g06780 protein kinase domain containing protein 0.17 0.68 0.16 2.02 Os10g06930 mitogen-activated protein kinase 15 -0.40 0.26 0.91 2.23 Os11g02300 CPuORF36 - conserved peptide uORF-

containing transcript 0.31 0.49 0.09 1.20

Os12g02250 STE_PAK_Ste20_Slob_Wnk.3 -0.79 0.16 0.03 1.90 Os09g31160 glutamate receptor precursor -0.34 -0.21 0.09 1.04 Os05g02940 calcium-transporting ATPase 2, endoplasmic

reticulum-type 0.22 0.14 0.41 1.13

Os09g11450 transporter, monovalent cation:proton antiporter-2 family

0.37 0.37 0.19 1.03

Os08g40530 calcium-transporting ATPase 9, plasma membrane-type

0.05 0.26 0.34 1.10


-0.85 -0.19 -0.41 1.81


0.26 0.43 0.32 1.00


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Supplemental Table 2. Response of Selected Salt-Responsive Genes to H2O2, ABA and Mannitol Treatment. Data represent mean ± SE (∆∆CT) of roots treated with 5 mM H2O2, 5

µM ABA or 100 mM mannitol for 30 min or 3 h relative to mock-treated roots from three

independent biological replicates. Statistically significant differences were determined by the

Student's t-test. Bold font indicates a significant up- or down-regulation (∆∆CT ≥ +1 or ≤ -1

with p ≤ 0.05) relative to the expression in mock-treated roots.

H2O2 ABA mannitol MAPK signalling components 30min 3h 30min 3h 30min 3h Os03g17700 MAPK5 1.85 2.41 -0.14 0.57 0.36 -0.17

Os01g47530 MAPK8 1.63 1.92 -0.57 0.85 -1.27 0.28

Os05g50560 MAPK9 0.81 0.84 -0.05 0.72 0.58 2.33 Os11g17080 MAPK15 0.82 1.58 -1.07 0.08 1.49 2.90 Os03g12390 MAP2K6 0.42 1.40 -1.68 0.36 -0.58 0.01

Os01g50370 MAP3K4 0.32 0.41 -0.19 -0.48 -0.02 -0.35

Os01g50410 MAP3K6 0.26 3.83 -0.83 -0.08 n.d. -0.38

Os03g15570 MAP3K12 0.77 1.23 -1.23 -0.78 -0.82 0.14

Os03g55560 MAP3K15 0.08 -0.73 -0.77 -0.97 -0.84 0.32

Os05g46750 MAP3K18 2.45 4.62 -0.32 4.52 -0.28 -1.83 Os05g46760 MAP3K19 3.02 4.68 -0.44 1.18 -0.08 -0.20

Os10g04010 MAP3K23 -0.38 2.94 -0.65 -0.64 -2.20 -1.57

Os06g43030 protein kinase -0.86 2.55 -1.85 0.39 -1.11 -0.17

Os07g43900 protein kinase -0.18 -0.76 -0.61 0.05 -0.78 0.14

Calcium-dependent signalling components

Os02g03410 CPK4 3.60 3.68 -0.66 0.63 0.62 0.83

Os02g58520 CPK6 3.26 1.54 -0.62 -1.26 2.06 0.65

Os05g39090 CPK16 0.42 2.26 -0.18 0.77 0.01 2.32

Os07g44710 CPK30 -0.26 -1.30 -0.84 0.07 -0.35 0.71

Os07g48760 CIPK3 1.09 2.74 0.09 0.77 -0.01 1.09

Os12g41090 CIPK4 -2.06 -3.05 -2.47 -2.11 -1.35 -0.45

Os08g34240 CIPK6 -0.02 4.64 0.91 -0.05 0.83 0.02

Os05g26820 CIPK18 -0.66 2.17 -0.60 0.66 0.83 3.51 Os05g26940 CIPK22 -1.61 -2.00 0.51 3.77 -0.18 -1.98 Os02g18930 CBL8 -0.98 -2.95 -2.09 -2.65 -1.58 -0.21

Os01g51420 CBL10 -0.58 1.41 -0.18 1.99 -0.87 0.64

Channels

Os01g45990 AKT1 -1.18 1.81 -0.13 1.07 -1.65 -1.77 Os02g14840 KAT1 0.56 1.46 -0.10 2.06 -3.12 0.07

Os05g35410 AKT2/3 -0.18 1.50 -0.14 -0.02 0.44 -0.61

Os04g36740 SKOR -0.44 -1.14 -0.94 -2.80 -1.17 -0.58

Os06g14030 SKOR -0.38 0.08 -0.46 0.94 -1.53 -0.68

Os03g55100 CNGC2 -0.38 -2.59 -0.05 0.47 -0.88 -0.88

Os06g08880 GLR2.7 -0.02 -1.95 -0.19 -0.13 0.00 0.33

Os06g08930 GLR2.8 -0.71 1.04 -0.53 -0.93 -2.26 -2.74 Os04g49570 GLR3.1 0.01 -0.93 -0.66 -1.53 -0.06 -0.23

Os06g46670 GLR -0.28 -0.13 -0.55 -1.22 -0.54 0.18

Os07g01310 GLR -1.32 -0.29 -0.83 -1.82 -1.30 0.74

Os09g26160 GLR 1.96 1.94 0.17 0.15 -0.01 1.00

Os09g31160 GLR -0.48 -0.28 -0.58 -0.98 -0.93 0.38

ATPases

Os03g17310 ECA1 -0.33 0.49 -0.44 0.23 -1.72 -0.27


14

Os05g41580 ACA7 0.13 3.14 -0.49 -1.40 -0.16 0.05

Os03g48310 AHA1 0.26 3.27 -0.03 4.22 -0.64 0.63

Os05g51530 VHA-C -0.26 -0.63 -0.56 0.45 -0.57 0.06

Os01g73130 VHA-c4 0.26 2.25 -0.97 -1.17 -3.20 0.46

Os02g55890 OVP4 -0.18 -1.12 -0.60 -0.42 -0.71 -0.29

Transporters

Os06g48810 HKT1 -3.43 -3.33 -0.55 2.92 -1.53 -1.41 Os02g07830 HKT6 -0.78 -0.26 0.02 -1.18 0.51 0.55

Os04g51830 HKT7 -2.79 -0.95 -1.76 -1.63 -3.79 -2.23 Os01g20160 HKT8 -0.03 0.05 -1.09 2.03 -0.86 0.99

Os01g70940 HAK2 -0.16 -0.31 0.09 3.27 -0.81 0.19

Os08g36340 HAK4 0.56 n.d. -0.43 -1.31 1.30 0.89

Os01g70660 HAK6 0.12 -0.62 -1.12 -1.83 0.25 -0.25

Os06g45940 HAK13 -1.78 -4.37 -1.19 -2.51 -1.22 -0.18

Os09g38960 HAK18 -0.86 -0.41 -0.46 1.61 -0.58 0.24

Os06g15910 HAK24 -1.03 0.61 -0.41 1.99 -0.48 -0.47

Os05g51610 CAX1b 1.38 -0.41 -1.25 -1.91 3.88 3.49 Os11g01580 CaCA family -0.54 1.83 0.38 -0.92 -2.22 0.64

Os12g42910 CaCA family 0.34 3.60 1.73 5.72 -0.66 -1.71 Os09g30446 CPA1 -0.50 -2.58 -1.68 -0.35 0.77 0.15

Aquaporins

Os02g44080 TIP2-1 -1.31 -2.37 -1.38 -2.27 -1.48 -0.82

Os04g44570 TIP3-2 0.89 1.51 0.93 n.d. -0.70 1.10

Os01g13130 TIP4-2 0.06 -0.41 0.39 2.20 -0.74 -1.03

Os01g13120 TIP4-3 -0.14 0.80 0.30 -1.32 -1.07 -0.04

Os02g57720 PIP1-3 -0.20 0.08 -0.53 0.69 -1.13 0.25

Os07g26690 PIP2-1 -0.67 -0.20 -0.89 -0.08 -1.41 -0.19

Os02g41860 PIP2-2 -0.25 -1.55 -0.35 0.62 -1.28 -0.11

Os04g44060 PIP2-3 -0.84 -1.05 -0.76 -0.05 -1.46 0.13

Os07g26630 PIP2-4 -0.89 -2.35 -0.76 -1.69 -1.38 -0.22

Os02g13870 NIP1-1 0.00 3.65 -0.75 -1.70 -0.29 0.76

Os06g12310 NIP2-2 0.69 -2.17 1.79 -0.73 3.03 2.68 Os07g26640 aquaporin -0.79 -0.60 -0.90 -1.50 -1.14 0.26


15

Supplemental Table 3. Expression of Selected Salt-Responsive Genes in SERF1 Knock-Down and Overexpression Lines. Comparison of

expression levels (log2FC) in roots under non-stress and salt stress conditions (100 mM NaCl). n = 3. The values that are significantly different

from control (∆∆CT ≤ -1 or ≥ +1; Student´s t-test) are set in bold type (p ≤ 0.05). Down-regulated and up-regulated genes are marked in blue and

orange, respectively. FC, fold change; EV, empty vector; n.d., not determined.

mock 100 mM NaCl 30 min 30 min 30 min 30 min 30 min 3 h 3 h 3 h

KD 4-1 KD 5-1 OE 14-1 OE 27-1 EV KD 4-1 KD 5-1 OE 14-1 OE 27-1 EV OE 14-1 OE 27-1 MAPK-signalling components

Os03g17700 MAPK5 0.04 0.19 2.12 1.50 1.01 0.25 0.64 -0.11 2.85 2.68 3.70 6.42 Os01g47530 MAPK8 -0.98 -0.65 0.60 -0.82 0.17 0.52 -0.14 1.89 1.34 -0.83 1.03 0.38

Os05g50560 MAPK9 -0.48 0.29 -0.61 -1.90 0.37 0.26 -0.10 0.70 1.39 -0.86 1.07 0.47

Os11g17080 MAPK15 -0.42 -0.40 0.29 -0.43 -1.21 -0.21 0.02 -0.43 -0.20 -0.22 -0.04 0.56

Os03g12390 MAP2K6 0.53 0.16 -0.35 -0.47 -0.50 -0.76 -0.93 0.94 0.77 0.68 1.81 2.17 Os01g50370 MAP3K4 0.28 -0.05 0.87 -0.12 1.86 0.96 1.52 1.07 0.70 0.47 1.72 1.89 Os01g50410 MAP3K6 0.17 -0.08 1.02 0.02 1.48 1.10 0.90 1.76 1.58 2.59 3.56 3.90 Os03g15570 MAP3K12 0.41 0.72 -0.73 -1.53 1.00 -0.15 0.36 1.21 1.12 -0.50 1.08 1.22

Os03g55560 MAP3K15 -0.47 0.41 -0.64 -1.29 0.17 0.32 0.25 1.19 1.02 0.05 1.70 1.40

Os05g46750 MAP3K18 -5.98 -6.97 0.57 -0.14 -1.93 -0.39 1.39 1.04 2.22 2.29 2.51 3.72 Os05g46760 MAP3K19 0.73 -0.29 -0.17 -0.67 1.81 0.45 0.46 0.02 0.53 -0.62 -1.41 1.79 Os10g04010 MAP3K23 2.78 n.d. 2.49 -1.19 8.96 -1.22 1.81 -0.03 0.41 -0.80 -0.56 -0.84

Os06g43030 protein kinase

0.67 0.84 -0.74 -0.97 0.56 -0.15 0.14 0.10 1.48 2.95 3.92 3.99

Os07g43900 protein kinase

-0.02 -0.41 0.22 -0.21 0.10 -0.20 -0.24 -0.35 -0.29 0.31 0.53 0.78

Calcium-dependent signalling

Os02g03410 CPK4 0.39 0.32 0.10 -0.24 1.67 1.10 1.57 -0.30 -0.22 -0.59 -0.11 -0.21

Os02g58520 CPK6 -0.60 -0.03 -0.17 -1.78 -0.31 0.26 -0.07 1.32 1.26 -2.27 -1.61 0.56

Os05g39090 CPK16 0.28 0.71 -0.35 -0.60 0.28 -0.23 -0.58 0.06 0.20 0.52 1.13 0.99

Os07g44710 CPK30 -0.03 0.49 -0.71 -0.91 0.26 -0.22 -0.36 0.02 0.36 0.07 1.28 0.76

Os07g48760 CIPK3 0.25 -0.31 0.77 -0.40 0.46 0.16 1.40 -1.89 -0.65 -1.95 -2.61 -1.74 Os12g41090 CIPK4 0.18 -0.32 -3.24 -3.45 -1.45 -0.88 -0.88 -2.67 -1.26 -2.72 2.40 2.90 Os08g34240 CIPK6 0.70 -0.75 -2.04 -1.68 1.38 0.58 0.33 -0.10 0.74 0.46 2.26 1.45 Os05g26820 CIPK18 0.02 -1.40 -0.62 -0.92 -1.51 -0.83 0.36 -1.05 -0.46 -1.02 -0.15 -0.24


16

Os05g26940 CIPK22 0.35 0.56 -0.52 -0.77 0.03 -0.88 -0.77 -1.01 -0.24 -0.23 0.27 0.20

Os02g18930 CBL8 -0.84 -0.52 0.48 -0.40 -1.20 -0.36 -0.79 -1.85 -0.44 -1.60 -1.80 -0.45

Os01g51420 CBL10 -0.07 -0.09 -0.36 -1.16 -0.54 -0.53 -0.27 -1.12 0.62 1.86 1.15 1.87

Channels

Os01g45990 AKT1 0.29 -0.55 0.40 -0.62 1.12 -0.76 0.86 -0.65 0.32 -1.47 -1.27 -1.74

Os02g14840 KAT1 -1.81 -0.97 -0.02 -0.74 -0.74 -0.75 0.45 0.10 1.08 0.49 0.86 1.86

Os05g35410 AKT2/3 1.97 0.84 -3.65 -1.75 1.33 nd -0.57 1.32 -2.23 -1.81 0.52 1.14

Os04g36740 SKOR -1.70 -0.88 0.79 -1.03 -1.73 -0.91 -0.79 -0.67 -0.63 -3.67 -4.02 -1.96

Os06g14030 SKOR -0.73 0.49 0.79 -0.28 -0.60 0.02 -0.82 -0.92 1.45 1.63 0.75 1.32

Os03g55100 CNGC2 0.18 -0.35 -0.02 -1.67 2.64 -0.97 -0.45 -0.31 1.33 -0.14 -0.10 1.21

Os06g08880 GLR2.7 -0.18 -2.09 -0.10 -0.61 0.53 -2.17 -0.04 -0.11 -1.22 -1.81 -1.24 -1.85

Os06g08930 GLR2.8 -0.91 0.08 -1.28 -3.50 -1.05 -0.25 -0.16 0.20 0.42 -3.08 -1.05 0.19

Os04g49570 GLR3.1 -0.24 0.18 -0.24 -0.48 0.69 -0.89 -0.15 -0.17 0.76 0.38 0.99 1.43

Os06g46670 GLR 0.69 -0.43 -0.18 -0.21 0.39 -1.13 -0.12 -0.71 -0.67 -1.72 -1.27 -0.56

Os07g01310 GLR -0.44 -1.45 -1.28 -0.98 0.45 -0.68 0.39 -4.37 2.54 -0.35 -1.54 2.32

Os09g26160 GLR 0.53 0.26 0.31 -1.02 2.71 0.07 1.35 -1.45 -0.34 1.41 0.96 1.67

Os09g31160 GLR -0.14 -0.22 -0.36 -0.35 0.73 -0.54 -0.19 0.43 0.88 1.32 2.02 2.03 ATPase

Os03g17310 ECA1 0.05 0.37 -6.17 -6.51 -0.27 -0.31 -0.39 -2.03 1.27 -4.91 0.13 1.17

Os05g41580 ACA7 0.37 -0.48 -0.03 -0.75 1.78 0.16 1.16 -0.54 -0.54 -1.04 -1.17 -0.31

Os03g48310 AHA1 -0.14 0.58 -0.12 -1.02 0.13 0.26 -0.11 -0.95 1.19 0.01 0.65 0.78

Os05g51530 VHA-C 0.16 -0.54 n.d. n.d. 0.02 -1.07 -0.12 n.d. n.d. n.d. n.d. n.d.

Os01g73130 VHA-c4 3.49 -3.24 -4.47 -4.50 0.26 -0.50 -0.30 0.03 0.21 -1.22 0.67 3.34 Os02g55890 OVP4 0.25 0.70 -0.51 -1.49 0.12 0.32 0.13 -1.22 0.69 0.39 0.95 1.93

Transporters

Os06g48810 HKT1 0.49 -0.65 0.22 -0.61 0.27 -0.48 0.04 -2.18 -1.34 0.49 -0.55 -1.63

Os02g07830 HKT6 1.15 -0.79 -1.50 -0.85 0.40 n.d. -0.44 -0.45 -0.78 -2.11 -1.04 -2.65

Os04g51830 HKT7 0.86 -0.09 0.02 -0.51 -0.07 -1.44 -0.34 -0.56 0.70 -3.07 -2.09 -1.87

Os01g20160 HKT8 -0.07 0.64 -1.83 -5.40 -0.97 -0.78 -0.61 0.53 1.15 -7.89 -4.44 -2.52

Os01g70940 HAK2 0.03 0.27 -0.03 -1.53 -0.69 -0.46 -0.70 -2.36 -0.85 -1.11 0.02 2.02

Os08g36340 HAK4 0.72 1.67 2.15 -0.49 -0.55 -0.99 -0.66 -2.91 1.34 -2.23 -3.52 -0.53

Os01g70660 HAK6 0.03 -0.95 -1.97 -1.47 0.65 0.92 0.35 -2.04 -2.88 0.33 1.23 0.59

Os06g45940 HAK13 -0.15 1.18 -0.76 -1.91 -0.50 -0.85 -0.82 -0.03 0.56 -2.39 -1.50 0.35

Os09g38960 HAK18 -0.22 0.41 -0.86 -1.19 -0.48 -0.19 -0.79 -0.79 0.45 0.84 1.80 1.64 Os06g15910 HAK24 0.13 -0.75 2.18 0.65 -1.52 -1.96 -2.33 -0.91 -0.29 4.91 2.85 3.36

Os05g51610 CAX1b -0.85 -1.47 -1.42 -1.77 0.46 -1.12 -1.42 -1.21 0.79 -0.85 -0.53 0.73


17

Os11g01580 Caca family 0.36 -0.35 -1.14 -4.14 -1.14 -1.81 -1.59 -0.90 -0.60 -3.01 -2.65 -0.96

Os12g42910 Caca family 3.07 -0.55 0.19 -0.82 1.06 -0.10 -0.33 -0.32 0.81 4.21 4.25 4.18 Os09g30446 CPA1 1.27 -1.23 -0.41 0.45 0.24 -1.08 0.19 -0.89 -2.07 -3.88 -1.28 -1.06

Aquaporins

Os02g44080 TIP2-1 -0.75 -0.44 0.49 -0.34 -0.53 -0.43 -1.00 -0.74 0.19 0.66 1.07 -0.09

Os04g44570 TIP3-2 -0.90 -0.31 -6.70 -7.16 0.54 0.22 -0.81 0.90 3.86 -0.67 4.36 5.36 Os01g13130 TIP4-2 0.16 0.36 0.09 -0.07 0.98 0.63 0.79 -1.70 -0.81 2.03 2.58 1.48

Os01g13120 TIP4-3 0.27 -0.38 0.02 0.00 0.72 -0.43 -0.63 -2.33 -1.70 -2.51 -2.46 -1.99 Os02g57720 PIP1-3 -0.06 -0.19 0.73 -0.36 -0.70 -0.41 -1.02 -1.38 -0.20 0.53 0.08 0.85

Os07g26690 PIP2-1 -0.23 -0.23 0.18 -1.06 -0.79 -0.52 -0.63 -0.87 0.14 0.64 0.77 1.44

Os02g41860 PIP-2-2 -0.18 -0.03 -0.42 -0.93 -0.89 -0.78 -1.45 -0.57 0.32 0.83 0.68 2.86

Os04g44060 PIP2-3 -0.42 -1.16 -0.52 -2.49 -0.44 -0.44 -0.95 -0.56 0.42 -0.59 0.43 1.63

Os07g26630 PIP2-4 -0.23 -0.30 0.28 0.16 -0.62 -1.02 -1.03 -0.19 -0.26 0.42 0.27 0.14

Os02g13870 NIP1-1 0.34 0.94 1.32 -0.31 0.52 0.67 0.01 -1.92 0.06 3.92 2.25 4.12 Os06g12310 NIP2-2 0.00 -1.55 1.77 -1.98 0.34 -0.13 0.38 -0.49 -0.28 -1.21 -0.68 -1.82

Os07g26640 aquaporin 0.12 0.43 0.43 -0.65 -1.22 -0.77 -1.00 -0.97 -0.02 0.73 0.48 0.92


18

Supplemental Table 4. Expression of Key Transcription Factor Genes in SERF1 Knock-down and Overexpression Plants. Comparison of

expression levels (log2FC) in roots of SERF1 knock-down and overexpression plants as well as EV plants under non-stress and salt stress

conditions (100 mM NaCl). Values are presented as the relative expression level (log2FC) of three biological replicates. The values that are

significantly different from control (∆∆CT ≤ -1 or ≥ +1) by Student´s t-test are set in bold type (p ≤ 0.05). Down-regulated and up-regulated genes

are marked in blue and orange, respectively. FC, fold change; EV, empty-vector control; n.d., not determined.

Locus EV EV KD 4-1 KD 5-1 KD 4-1 KD 5-1 OE 14-1 OE 27-1 OE 14-1 OE 27-1 OE 14-1 OE 27-1 30 min

salt 3 h salt control control 30 min

salt 30 min

salt control control 30 min

salt 30 min

salt 3 h salt 3 h salt

OsDREB2A 1.20 0.16 -1.05 -0.21 0.41 0.25 1.65 1.22 0.63 0.39 1.42 1.14 OsLea 1.85 10.00 -0.44 -0.30 -0.11 -0.44 -0.27 0.94 1.78 1.51 6.04 5.35 OsWRKY13 0.53 -0.07 0.21 0.44 0.20 0.28 0.44 -0.14 1.84 1.24 2.04 2.09 AP37 -0.17 3.41 0.77 0.37 -0.18 0.56 1.92 1.43 -1.17 -1.94 1.37 1.51 ZFP179 3.60 3.34 -0.25 -1.11 0.80 0.77 2.60 1.73 4.16 5.97 1.97 5.19 OsABI5 -0.17 -3.59 -0.73 -0.39 0.36 0.18 0.95 0.91 -0.27 0.25 3.76 2.72 SNAC2 -3.34 -0.60 -0.38 -1.81 0.18 0.42 1.34 0.93 -5.08 -0.89 2.39 2.94 OsGTc-1 2.84 -0.38 -0.66 0.63 -0.48 -1.45 0.24 0.12 5.70 7.42 2.69 2.68 OsBZIP23 -3.34 0.54 -0.49 -0.10 -0.34 -0.76 1.00 0.59 -6.77 -5.87 1.97 2.38 OsMAPK5 -0.45 2.68 0.04 0.19 0.25 0.64 2.12 1.50 -0.11 2.85 3.70 6.42 SNAC1 0.94 3.00 0.03 -1.27 0.14 0.73 0.72 0.20 1.13 1.75 2.69 3.97 ZFP182 1.95 2.62 0.09 -1.92 -0.25 0.07 2.35 1.65 3.53 3.15 3.41 3.60 OsP5CS -0.19 0.95 -0.07 0.55 0.17 -0.40 0.94 0.43 0.10 -0.24 1.07 1.25 OsLea3 3.99 3.17 -0.48 -0.81 -0.23 -0.83 3.25 0.24 3.54 3.82 6.35 6.64 TRAB1 1.70 -3.04 -0.58 -0.94 1.10 1.45 1.29 0.89 0.59 0.62 -0.77 -0.51

OsNAC5 -0.28 1.43 -0.08 0.06 -0.04 -0.68 0.15 -0.54 1.56 1.27 3.69 2.91 dehydrin 0.46 4.87 -0.18 -0.17 0.61 -0.13 2.71 3.08 0.32 1.27 7.97 6.04 ZFP252 -0.61 0.10 -0.05 -0.51 0.48 0.33 1.28 -0.07 -0.50 -1.34 0.59 -0.12


19

Supplemental Table 5. Putative Direct SERF1 Target Genes Identified. Listed are genes which fulfill the following criteria: presence of at least

one copy of the DREB-specific cis-element '(A/G)CCGAC' in the 1-kb promoter fragment directly upstream of the transcriptional start site, induction

in wild-type roots stressed with 100 mM NaCl for 30 min or 3 h, but lack of or weakened response in stressed serf1 roots, induction upon H2O2

treatment (5 mM) for 30 min or 3 h. Among those genes which furthermore were induced by MKK4DD in rice protoplasts (Kishi-Kaboshi et al., 2010)

four putative direct SERF1 targets were selected for binding studies. Expression data are given as log2FC and represent the means of three

independent biological replicates. Bold font indicates a significant up- or downregulation (∆∆CT ≥ +1 or ≤ -1 with p ≤ 0.05) relative to the expression

in mock-treated roots. * selected for binding studies; ↑ significantly induced (log2FC ≥ +1 or ≤ -1 with p ≤ 0.05) after 30 min or 3 h of treatment; a

induction of expression (+) by overexpression of the constitutively active form of MKK4 (MKK4DD) according to Kishi-Kaboshi et al. (2010).

Locus Name DRE WT 100 mM NaCl serf1 100 mM NaCl WT 5 mM

H2O2

MKK4DDa

(A/G)CCGAC 30 min 3 h 30 min 3 h

Os03g17700* MAPK5 -282; -50 2.20 0.98 0.78 -0.15 ↑ +

Os01g47530 MAPK8 -474 0.93 1.61 0.46 0.83 ↑

Os11g17080 MAPK15 -889; -461; -413 -0.53 1.15 -0.17 0.65 ↑

Os01g50410* MAP3K6 -814 2.60 0.83 1.54 0.16 ↑ +

Os05g46760 MAP3K19 -911 3.32 1.54 0.88 -0.37 ↑ +

Os01g07120* DREB2A -962; -661; -656 1.56 1.02 0.86 0.15 ↑ +

Os03g60080 SNAC1 -258; -89 3.28 1.41 1.88 0.11 ↑

Os01g62190* ZFP179 -898 2.64 0.93 0.98 -1.48 ↑ +

Os03g60560 ZFP182 -96 4.63 2.31 1.92 -0.21 ↑

Os05g41580 ACA7 -794 1.15 0.34 -0.14 -0.53 ↑ +


20

Supplemental Table 6. Sequences of Oligonucleotides Used for Cloning Constructs. PT, plant transformation; TA, trans-activation assay; SLA,

split-luciferase assay; KA, kinase assay; NS, no stop codon; OE, overexpression; FL full length.

Oligo Sequence 5'→3' Construct Assay MAPK5_CDS_exp_F TTTGCGATCGCATGGACGGGGCGCCGGTGGC MAPK5 CDS in vitro expression KA

MAPK5_CDS_exp_R TTAGTTTAAACCTAGTACCGGATGTTTGGGTTCAT MAPK5 CDS in vitro expression KA

SERF1_CDS_exp_F TTTGCGATCGCATGGGCCGCGTGGCCGCTT SERF1 in vitro expression KA /EMSA

SERF1_CDS_exp_R TTAGTTTAAACTCACATGAGGATTTCCATGTC SERF1 in vitro expression KA /EMSA

amiSERF_PI AGTGTGTCGCTAACCTCGCCAGGCAGGAGATTCAGTTTGA amiRNA SERF1 PT

amiSERF_PII TGCCTGGCGAGGTTAGCGACACACTGCTGCTGCTACAGCC amiRNA SERF1 PT

amiSERF_PIII CTCCTGGGGAGCTTAGCGACACATTCCTGCTGCTAGGCTG amiRNA SERF1 PT

amiSERF_PIV AATGTGTCGCTAAGCTCCCCAGGAGAGAGGCAAAAGTGAA amiRNA SERF1 PT

amiG4368_F CTGCAAGGCTAAGTTGGGTAAC all amiRNA construct PT

amiG4369_R GCGGATAACAATTTCACACAGGAAACAG all amiRNA construct PT

ATTB1_F GGGGACAAGTTTGTACAAAAAAGCAGGCTCAGCAGCACCACAGCAAAA all amiRNA construct PT

ATTB2_R GGGGACCACTTTGTACAAGAAAGCTGGGTGCTGCTGATGCTGATGCCAT all amiRNA construct PT

entrS1ns_R CATGAGGATTTCCATGTCGTC SERF1 CFP fusion NS pENTR PT

pSERF1_F caccTGAGCTACAATTAGGAGTCTGAT SERF1 promoter in pENTR PT / TA

pSERF1_R GTACGTACCGTCTTCCCCCTCC SERF1 promoter in pENTR PT / TA

entrS1_F caccATGGGCCGCGTGGCCGCTTCT SERF1 CDS OE FL pENTR PT / TA / SLA

entrS1_R TTACATGAGGATTTCCATGTCGTC SERF1 CDS OE FL pENTR PT / TA / SLA

pMAPK5_F caccTAACACGTCAGTTCTCCATCCACT MAPK5 promoter in pENTR TA

pMAPK5_R GTAGACTCTGCACCACCCTGTA MAPK5 promoter in pENTR TA

pDREB2A_F caccGTAATGAAATTCGATTATCGAAA DREB2A promoter in pENTR TA

pDREB2A_R CGGCCCTAAGAATAAAATACTTCTT DREB2A promoter in pENTR TA

pZFP179_F caccGGCGAAGTGGTCGTTGTCCCACA ZFP179 promoter in pENTR TA

pZFP179_R TCCCTTCACGTTGGCGGACGTA ZFP179 promoter in pENTR TA

SERF1_Asp105_CDS_R TGCACAGATCTTGGGTCGAGCA SERF1D105

CDS in pENTR TA

SERF1_Ala105_CDS_R TGCACAGATCTTGGGGCGAGCA SERF1A105

CDS in pENTR TA

MAPK5_CDS_F caccATGGACGGGGCGCCGGTGGC MAPK5 CDS in pENTR TA

MAPK5_CDS_R TAGTACCGGATGTTTGGGTTCAT MAPK5 CDS in pENTR TA


21

Supplemental Table 7. Sequences of Oligonucleotides Used for Expression Profiling of 373 Genes Involved in the Salt Stress Response.

Locus Name Oligonucleotide sequence 5'→3' GC content (%)

Tm (°C)

Os01g02190 aquaporin protein Os01g02190-F TAGAGCAAGCTCGCCATGATCTGC 54.2 64.4

Os01g02190-R AACACCACCAAGAACGACGCCAAC 54.2 64.4

Os01g08660 aquaporin protein Os01g08660-F ATCGTCAAGACCTGGATGCTCTCC 54.2 64.4

Os01g08660-R ACATATGCCCAGCCAAACGCATTG 50 62.7

Os01g10450 protein kinase domain containing protein Os01g10450-F TGTTGTTGCTGCTGCGTTTGCG 54.5 62.1

Os01g10450-R TCAAAGCTCCCACCGGAAAGATGG 54.2 64.4

Os01g10600 aquaporin protein Os01g10600-F TCTTCCTCATGTTCGTCGTCTCCG 54.2 64.4

Os01g10600-R CCAGCCAATTCACCGATTGCTCTG 54.2 64.4

Os01g10870 CAMK_KIN1/SNF1/Nim1_like.7 Os01g10870-F ACGAGTGGTTCAAGATCGGCTTCC 54.2 64.4

Os01g10870-R AAGTTCGAAGCAGGTGAAGGTGCG 54.2 64.4

Os01g10890 CAMK_KIN1/SNF1/Nim1_like.8 Os01g10890-F TCCCCCCATTTGCTTCTTTTGCAC 50 62.7

Os01g10890-R ATGGACGCCTTCTTCTCCATCCTG 54.2 64.4

Os01g11250 potassium channel KAT1 Os01g11250-F TGCCGGACCAGGAATTTTAGGGAC 54.2 64.4

Os01g11250-R TCCAGGCAACTGATTTCTTGCAGC 50 62.7

Os01g11414 sodium/calcium exchanger protein Os01g11414-F ATGCGAGAACAAGGCAGGAACACG 54.2 64.4

Os01g11414-R TCTGCGAATGCAGCAGCAATGG 54.5 62.1

Os01g12260 vacuolar ATP synthase subunit E Os01g12260-F CCCGACTTGAAGTTCTGCAAGCTC 54.2 64.4

Os01g12260-R TTCCCTGCTGCCTCCAACATAGAC 54.2 64.4

Os01g13120 aquaporin protein Os01g13120-F TTTGGTTCTGGGTCAAGCCTTCC 52.2 62.4

Os01g13120-R CCGGACCTGCCCTTTTAAGCATTC 54.2 64.4

Os01g13130 aquaporin protein Os01g13130-F CCAACCATTTCGGCATATCGTTCG 50 62.7

Os01g13130-R AGCAACGGCATAGACCGATCAC 54.5 62.1

Os01g18800 CAMK_KIN1/SNF1/Nim1_like.9 Os01g18800-F CCAAGCCCAAAAGACGAACGAAGG 54.2 64.4

Os01g18800-R TGCTGCTATTGTCTGCTTGCGTTG 50 62.7

Os01g20160 OsHKT1;5 - Na+ transporter Os01g20160-F TCTCGTGCCTAGCCATCTTCATCG 54.2 64.4

Os01g20160-R TACGCACTGATAACCTCGACGACG 54.2 64.4

Os01g23580 inorganic H+ pyrophosphatase Os01g23580-F TCGTCTTCGCGCCATTCTTTGC 54.5 62.1

Os01g23580-R TGCTTCAGCTTGCTCTTCGTTCAC 50 62.7

Os01g27170 potassium transporter Os01g27170-F AAATCACCCCACTGCGACATCCAC 54.2 64.4

Os01g27170-R CAGAAGATGCCCGTTGGAATGGAC 54.2 64.4

Os01g32660 STE_MEK_ste7_MAP2K.2 Os01g32660-F CAGCGGGATACGTTTGTTGGAACC 54.2 64.4


22

Os01g32660-R TATAGGGGAACCGACCAATGGCAC 54.2 64.4

Os01g34850 OsHKT2;3 - Na+ transporter Os01g34850-F TGATTGTTCCCTTGTTGCGCCAG 52.2 62.4

Os01g34850-R TGGGCCGATTCGTTTGTGATCTTG 50 62.7

Os01g35184 CAMK_KIN1/SNF1/Nim1_like.10 Os01g35184-F ATGATCCTGAGGAAGACGCTGACC 54.2 64.4

Os01g35184-R CGGCGATCAAATAGTGCTGCAAGG 54.2 64.4

Os01g37690 sodium/calcium exchanger protein Os01g37690-F GCGCTCAAGAACAAGCTGGACATC 54.2 64.4

Os01g37690-R GATCCATTTGGACCCCCATGATCC 54.2 64.4

Os01g39770 calcineurin B Os01g39770-F AAGATCCCCGGATGATGGTGACAG 54.2 64.4

Os01g39770-R ACATGCAGCACAAAGTTGGCAGG 52.2 62.4

Os01g40470 vacuolar ATP synthase subunit d Os01g40470-F GCGCAGAATCAGAAGAACAGGGTC 54.2 64.4

Os01g40470-R GTGGCAGGAGCAGGTCAAAAGATG 54.2 64.4

Os01g40570 outer mitochondrial membrane porin Os01g40570-F ACTCCGTTTTAACCGCCTTCGC 54.5 62.1

Os01g40570-R AGCAGATCTCTGGTCTTCTTGCCG 54.2 64.4

Os01g41510 calcineurin B Os01g41510-F AATCGCGCCCAAGAATCCATCTCC 54.2 64.4

Os01g41510-R AGCCGAGCGCAAATCTTAAGCAC 52.2 62.4

Os01g42430 vacuolar ATP synthase Os01g42430-F TCTTGTTTGCGGGGTTTGTGTGG 52.2 62.4

Os01g42430-R AAGGCGCTGCCGAAGATCTCAATC 54.2 64.4

Os01g43410 CAMK_CAMK_like.9 Os01g43410-F CGCCATGATGAGGAACAACAACCC 54.2 64.4

Os01g43410-R AAGGAAGTTAGAACATGCGCCGCC 54.2 64.4

Os01g43910 CGMC_MAPKCMGC_2.4 Os01g43910-F ACCAAGAGCACAGGGCAATCCAAG 54.2 64.4

Os01g43910-R GGGCACCAATGCAACTTCTTAGGC 54.2 64.4

Os01g45620 CGMC_MAPKCMGC_2.5 Os01g45620-F TTTGTGACTTCGGCCTTGCTCG 54.5 62.1

Os01g45620-R CTTGTTGCCACGTAATCCGTCCAG 54.2 64.4

Os01g45990 potassium channel AKT1 Os01g45990-F TACGACCGCCGATACAGAATCTGG 54.2 64.4

Os01g45990-R AATGGTGAGACCCACGCAGAGTAG 54.2 64.4

Os01g46980 vacuolar ATP synthase subunit E Os01g46980-F TATTTGCCTGTTGTCCCTGGCCTG 54.2 64.4

Os01g46980-R GGCCCACACCAGAGTTACACAAAC 54.2 64.4

Os01g47530 CGMC_MAPKCMGC_2.6 Os01g47530-F GATTGTCAGAACTGACCGCACCAC 54.2 64.4

Os01g47530-R GCTCATGCTGTAAGCCTGTAACCG 54.2 64.4

Os01g48330 protein kinase domain containing protein Os01g48330-F TTTGGAGGCGAAGCCAGAGAAGAG 54.2 64.4

Os01g48330-R ATGCTCAGGGCTTGAACTAGCACC 54.2 64.4

Os01g48680 two pore calcium channel protein 1 Os01g48680-F TTCCATATCGGCGTAACGCTCCTG 54.2 64.4

Os01g48680-R GTGTGAGTGGATGCAGATGTGCAG 54.2 64.4

Os01g50120 expressed protein Os01g50120-F AAGCCGACCTCACCCAATCCAAAG 54.2 64.4

Os01g50120-R TCAGTCAACCGCACAAGCCTTACC 54.2 64.4

Os01g50370 STE_MEKK_ste11_MAP3K.4 Os01g50370-F CAAGCCGCTGATCGTTCAATCGAG 54.2 64.4


23

Os01g50370-R ACATCATTGTCTGAACCAGCGCAG 50 62.7

Os01g50400 STE_MEKK_ste11_MAP3K.5 Os01g50400-F ATGCAGAGTGTGTTGATCCCGAGG 54.2 64.4

Os01g50400-R TGCTGCCCAATGGAAGCAACAG 54.5 62.1

Os01g50410 STE_MEKK_ste11_MAP3K.6 Os01g50410-F ACCTGCACGTACATCTTGGCAGTG 54.2 64.4

Os01g50410-R ATCAAGCCCTGATTCAGTCCCGTC 54.2 64.4

Os01g50420 STE_MEKK_ste11_MAP3K.7 Os01g50420-F GCTCCCGCGCATAGTTTCTTGTAG 54.2 64.4

Os01g50420-R TGTGTGAATAGTCGGGCTGAGGTG 54.2 64.4

Os01g51380 ATP synthase Os01g51380-F ACTTGGCATATGAGTGCGGGAAGC 54.2 64.4

Os01g51380-R AGAAACTTCACGAAGTGCATCGGC 50 62.7

Os01g51420 calcineurin B Os01g51420-F TCGATGACGGCCTGATCCATAAGG 54.2 64.4

Os01g51420-R TTCCAGCCGGTGTTCTAAACAAGG 50 62.7

Os01g52070 potassium channel AKT1 Os01g52070-F AAGCTCCTCTACGTGACGCTGTTC 54.2 64.4

Os01g52070-R AACGCCATCCACAGGTAGATGCAC 54.2 64.4

Os01g54480 serine/threonine protein kinase Os01g54480-F CACCGTGACCTTGAGCCTTCAAAC 54.2 64.4

Os01g54480-R TGCCACCTTCAGATGTCCAGTGTC 54.2 64.4

Os01g55200 potassium channel KAT1 Os01g55200-F CGCCCTGGACCGAAATATTATGCC 54.2 64.4

Os01g55200-R TCGTCGAAACACGGGAAGCAATG 52.2 62.4

Os01g55440 CAMK_KIN1/SNF1/Nim1_like.1 Os01g55440-F TGTGAAGGGTGATGGGTGAACCAC 54.2 64.4

Os01g55440-R AATCTGACAGCGGAATGCCAACG 52.2 62.4

Os01g55450 CAMK_KIN1/SNF1/Nim1_like.11 Os01g55450-F TTCAAGCTTACAGGCTCCCCTCAC 54.2 64.4

Os01g55450-R TGCATGGAAGCTCCTGTCTCTAGC 54.2 64.4

Os01g57370 cyclic nucleotide-gated ion channel 2 Os01g57370-F TTGGGAACTTGGCGAGCACAAC 54.5 62.1

Os01g57370-R GCCGCGTTCAAGAACACCTTGATG 54.2 64.4

Os01g59360 CAMK_CAMK_like.10 Os01g59360-F ACAGGTTGCACACACACACACAC 52.2 62.4

Os01g59360-R CATGCCATGAAACAGCAGCAAGC 52.2 62.4

Os01g60910 CAMK_KIN1/SNF1/Nim1_like.12 Os01g60910-F TACGCATGGTCACCAGGAAGAACG 54.2 64.4

Os01g60910-R CCAAGTACTGTTGTCACAGGCTGC 54.2 64.4

Os01g61590 CAMK_CAMK_like.1 Os01g61590-F TTTTGGGCTGAGACTGAGCAAGGG 54.2 64.4

Os01g61590-R ATCAGGCTCCAACATGCGTCGAAC 54.2 64.4

Os01g61780 vacuolar ATP synthase 98 kDa subunit Os01g61780-F CTCTGTTCGACCTGCAATGCAACC 54.2 64.4

Os01g61780-R CGCAATTTTGCCTCTAGCTCCTCC 54.2 64.4

Os01g66860 serine/threonine protein kinase Os01g66860-F ACCGATGATGGTGTCGAATCCTGG 54.2 64.4

Os01g66860-R AACTTGGTACACGCCCTTGACCAC 54.2 64.4

Os01g70300 aspartokinase 3, chloroplast precursor Os01g70300-F AAGCACGGCAGTACGACGCATTTG 54.2 64.4

Os01g70300-R TGTCCGCGTTTCCGAAATCGTCTG 54.2 64.4

Os01g70490 potassium transporter Os01g70490-F AATGACCGAGCCTCTGCACACAAG 54.2 64.4


24

Os01g70490-R ATGCAGCGTTTGGACCCCTTTC 54.5 62.1

Os01g70660 potassium transporter Os01g70660-F AGTACGCCCTCATCGTCCTCAAAG 54.2 64.4

Os01g70660-R ACAGTGCAAATGTACCACCTTCGC 50 62.7

Os01g70940 potassium transporter Os01g70940-F ACTGTCTTGTTGAGCAGCACGC 54.5 62.1

Os01g70940-R AGAGCAACAACGCCATCATCGC 54.5 62.1

Os01g71240 calcium-transporting ATPase, plasma membrane-type Os01g71240-F ACATGCGCCGCAACGAGATTAC 54.5 62.1

Os01g71240-R AATGAATGAACCGCCCGCCCTTTG 54.2 64.4

Os01g73130 vacuolar ATP synthase Os01g73130-F CATCGCTGTCATCATCACCACC 54.5 62.1

Os01g73130-R TCGAAGTGGTAGTACGGCATCG 54.5 62.1

Os01g74450 aquaporin protein Os01g74450-F GCGCTTAGCGTAACTAGACGAACG 54.2 64.4

Os01g74450-R CGGCTCACCGGCATTTTCTTCTTC 54.2 64.4

Os02g02540 glutamate receptor Os02g02540-F TAGATGAGCGCCCCTACGTTGAAC 54.2 64.4

Os02g02540-R GGAAATGCAAAGCCCCATCCTCTG 54.2 64.4

Os02g03410 CAMK_CAMK_like.12 Os02g03410-F ACCCCTGTGAACGTCGGTAAGAAC 54.2 64.4

Os02g03410-R GACACCCTCAACATCACATGGCAC 54.2 64.4

Os02g04230 CGMC_MAPKCMGC_2.7 Os02g04230-F TGCGCCTGCTAGAGCGTTTACTTG 54.2 64.4

Os02g04230-R TTGAAGGCTCCCGGCTCAACTTAG 54.2 64.4

Os02g04630 sodium/calcium exchanger protein Os02g04630-F GCAGACCTTTAGCAAGGCAGATGC 54.2 64.4

Os02g04630-R AACCCCATCACTGCCATCAAAAGC 50 62.7

Os02g05480 CGMC_MAPKCMGC_2_SLT2y_ERK.1 Os02g05480-F GGGAAATGATGTGGCACGAGATGC 54.2 64.4

Os02g05480-R TGGGGACAGTTGAAGATCATCGGG 54.2 64.4

Os02g06570 CAMK_KIN1/SNF1/Nim1_like.13 Os02g06570-F GCCGCCTGTAAAACATGTGACCAG 54.2 64.4

Os02g06570-R TGGCCTCTGATGGAACAAGCTCTG 54.2 64.4

Os02g07830 OsHKT1;3 - Na+ transporter Os02g07830-F ATGATGCACGCTGCAAGGATGC 54.5 62.1

Os02g07830-R TTCCCATTGTCGCTCCATTTCCCC 54.2 64.4

Os02g07870 ATP synthase Os02g07870-F TTCCATTTGCTGCTACCGAGGCTC 54.2 64.4

Os02g07870-R AAAACGACGACGGCGCGTATAG 54.5 62.1

Os02g08010 LTPL43 - Protease inhibitor/seed storage/LTP family protein precursor

Os02g08010-F AACGCCGTTCAGCTTCTTTGGG 54.5 62.1

Os02g08010-R TGGTTCAGTAGCCAATGCAAGTGC 50 62.7

Os02g08140 CAMK_KIN1/SNF1/Nim1_like.14 Os02g08140-F GGGGCTCGTGTTGTGTGATTTGAC 54.2 64.4

Os02g08140-R ATGCTCTCCACAAACCCCATCTCG 54.2 64.4

Os02g09150 inorganic H+ pyrophosphatase Os02g09150-F GCCGCAATTGGAAAGGGTTTTGCC 54.2 64.4

Os02g09150-R ATCAACCGTAGAGATAGCCGCACG 54.2 64.4

Os02g12810 protein kinase domain containing protein Os02g12810-F AGCTCGTGCAGAAGATTGCAAACC 50 62.7

Os02g12810-R TTCATCAGCATCATCCACCGGACC 54.2 64.4


25

Os02g13870 aquaporin protein Os02g13870-F TGCACCGCATCGCATTTAAAAGGG 50 62.7

Os02g13870-R AGCCGAGACGACAACGAACTAACG 54.2 64.4

Os02g14840 potassium channel KAT1 Os02g14840-F AGCCGCACCAGGAAATTTAGGGAC 54.2 64.4

Os02g14840-R AGCTGATTGCGTGCTGCAAACTC 52.2 62.4

Os02g14980 calcium-binding EF hand family protein Os02g14980-F ATGTTCAAGTCCGCCACCTACCTC 54.2 64.4

Os02g14980-R TTAGTGCCAGAGAGCCCAGAAACG 54.2 64.4

Os02g15580 cyclic nucleotide-gated ion channel 1 Os02g15580-F ACCTCAAGGAGCGAATACTGCGTC 54.2 64.4

Os02g15580-R AAGAAGGCCCTCTTCATCAACCCC 54.2 64.4

Os02g18880 calcineurin B Os02g18880-F AGCAAGCAGACTTAAACAGCGACG 50 62.7

Os02g18880-R TTGCGAACGCCTTCCATTCATCC 52.2 62.4

Os02g18930 calcineurin B Os02g18930-F TGCACGAAATGGTGCTTGCTCTTC 50 62.7

Os02g18930-R GCTTGCTTGAACGTCTGATCCACG 54.2 64.4

Os02g21009 sodium/calcium exchanger protein Os02g21009-F TGGCAATGGCAGTAATCACCACCG 54.2 64.4

Os02g21009-R ACGAAAAAGCACACCGCTATGACG 50 62.7

Os02g21700 STE_MEKK_ste11_MAP3K.8 Os02g21700-F ACGCTACCAACAACAACAACGACG 50 62.7

Os02g21700-R AGCAGAGGTGAGCTGAAAGCTTCC 54.2 64.4

Os02g27940 calcineurin B, putative Os02g27940-F TGGTTGCCACACTGGCTGAATC 54.5 62.1

Os02g27940-R CTGCCTCCTCAAATGTCTGGTCTG 54.2 64.4

Os02g29210 ankyrin Os02g29210-F AAACCTCCTGATTGAGGCTGGTGC 54.2 64.4

Os02g29210-R ACCCTTTGCAGCAGTTGAGATGC 52.2 62.4

Os02g31910 potassium transporter Os02g31910-F CAGGGCAATTCAGCTCAGCTTCAC 54.2 64.4

Os02g31910-R TCGCCGACATTCTCCGGGAATTTG 54.2 64.4

Os02g31940 potassium transporter Os02g31940-F AGCCTGACACAATTGCAAGTGGTG 50 62.7

Os02g31940-R ACCGAGAACAGCACGATCAGGATG 54.2 64.4

Os02g32610 protein kinase domain containing protein Os02g32610-F AAGGGAAATCACCACTCGCCTTGC 54.2 64.4

Os02g32610-R ATGCTACGGAACCCCCCATCTTTG 54.2 64.4

Os02g33490 inorganic H+ pyrophosphatase Os02g33490-F ATCTCTTTCGTTCCACCACCCCAC 54.2 64.4

Os02g33490-R TGCCGATGTTGTCCTTCCAACACC 54.2 64.4

Os02g35010 STE_MEKK_ste11_MAP3K.9 Os02g35010-F CAGGACTAAGCGCAAGGGAATTCG 54.2 64.4

Os02g35010-R TTGGGCTGCTGGAGGTATTGTGAG 54.2 64.4

Os02g38080 protein kinase domain containing protein Os02g38080-F ACATTACCTCCTGATGCACAGCGG 54.2 64.4

Os02g38080-R TCTTTGGGTATGGGCTATGGGCAC 54.2 64.4

Os02g41710 cyclic nucleotide-gated ion channel Os02g41710-F TCGCAACCGTCATTGGCGTTCTTG 54.2 64.4

Os02g41710-R CGCACCATCGTCGCTTGCATATTG 54.2 64.4

Os02g41860 aquaporin protein Os02g41860-F TTCGGCGGCATGATCTTCATCCTC 54.2 64.4

Os02g41860-R ACTGCGCGATGATGTACAGCAC 54.5 62.1


26

Os02g44080 aquaporin protein Os02g44080-F CGCCAAGCTCTCTTTCAAGCGTAG 54.2 64.4

Os02g44080-R AGGAGTCACCCAAGCTTCCGAATG 54.2 64.4

Os02g44630 aquaporin protein Os02g44630-F TTCAAGAGCAGGTCTTAAGCCCCG 54.2 64.4

Os02g44630-R TCACGATTGCGTTGCATGTCGTC 52.2 62.4

Os02g44642 STE_MEKK_ste11_MAP3K.10 Os02g44642-F TTCCCCTTGCTGCGAGCTTTTG 54.5 62.1

Os02g44642-R ACAACGCTTTCACTATGCAGGCTG 50 62.7

Os02g46090 CAMK_CAMK_like.15 Os02g46090-F AAGCATGAGATGAGACGCACCTGG 54.2 64.4

Os02g46090-R AGTTTCAAAGCTGCACTGTGGACG 50 62.7

Os02g46760 STE_MEK_ste7_MAP2K.4 Os02g46760-F GAGCTTCTTGGACACCCCTTCG 59.1 64.0

Os02g46760-R ACTCGTTTTGGCGCTCGCAAC 57.1 61.8

Os02g47320 vacuolar ATPase G subunit Os02g47320-F AACACCCCTTCGAGAATCGTCGTG 54.2 64.4

Os02g47320-R TGCTGAATTCCACCTTGGCGTCTG 54.2 64.4

Os02g49760 potassium transporter Os02g49760-F GTTGATGTTGGAGCACCTCATGGC 54.2 64.4

Os02g49760-R CCTGTTGGCTCTCCCAAGATTTGC 54.2 64.4

Os02g51110 aquaporin protein Os02g51110-F TGGAGCCTGCTAATTTTGGGCGTG 54.2 64.4

Os02g51110-R CGCACAATTTGACGGCAGAGACTG 54.2 64.4

Os02g53340 cyclic nucleotide-gated ion channel Os02g53340-F AGTGTCCAAATCGCGCACAAGTTC 50 62.7

Os02g53340-R TCGGGAGATTGCACCGATCAACAG 54.2 64.4

Os02g54510 protein kinase domain containing protein Os02g54510-F GCTGCTATTATTGGGGGCATCGTG 54.2 64.4

Os02g54510-R TATCTGCTGGTTCACTGGCCCAAC 54.2 64.4

Os02g54600 STE_MEK_ste7_MAP2K.5 Os02g54600-F TGCTTTGCTGTGCTGGCTTCTTG 52.2 62.4

Os02g54600-R AATCCAATCCAATCCACCCCCCAC 54.2 64.4

Os02g54640 glutamate receptor Os02g54640-F AAGTAGTGTGCAACCGTCTCCTCG 54.2 64.4

Os02g54640-R ACTGCCAATGCTTCATCGGTCAAG 50 62.7

Os02g54760 cyclic nucleotide-gated ion channel 14 Os02g54760-F AGCGAATTCACGAAGGGATCGAGG 54.2 64.4

Os02g54760-R TGGATGTACAAGTTGCCAACGCC 52.2 62.4

Os02g55400 ATPase 8, plasma membrane-type Os02g55400-F TCCAGCACCTGAAATGCAGCAAG 52.2 62.4

Os02g55400-R ATGTTGAGCCTGTTCTGTCCCTCC 54.2 64.4

Os02g55890 inorganic H+ pyrophosphatase Os02g55890-F TTGCAAAACAACCCACGCACTCC 52.2 62.4

Os02g55890-R TGGTTTGGGCGAGAGGTAGAAGAC 54.2 64.4

Os02g57720 aquaporin protein Os02g57720-F TGGCACGACCACTGGATTTTCTG 52.2 62.4

Os02g57720-R AAGGGGATTGCTCTGATCACCACC 54.2 64.4

Os02g57854 vacuolar ATP synthase subunit F Os02g57854-F TCCGTATGACCCAGCACACGATTC 54.2 64.4

Os02g57854-R TGCCACCGATTCAGCAGAAAACAG 50 62.7

Os02g58520 CAMK_CAMK_like.16 Os02g58520-F TGACGGAAGGAAACAGAGGGGAAG 54.2 64.4

Os02g58520-R TGCAACGAAACGACGATCCTCAAG 50 62.7


27

Os03g01120 E1-E2 ATPase domain containing protein Os03g01120-F AAGAGACATTGTAGGCGGCGAGAG 54.2 64.4

Os03g01120-R ACGACCCAACAACAAGGCAATCG 52.2 62.4

Os03g03510 CAMK_KIN1/SNF1/Nim1_like.15 Os03g03510-F CCCGGAGATGCACCTTGTATTTGC 54.2 64.4

Os03g03510-R AAGGCACACAACCCTACTGGTCAC 54.2 64.4

Os03g03660 CAMK_CAMK_like.17 Os03g03660-F GAATGGGGAATCAGTGCCAGAACG 54.2 64.4

Os03g03660-R ATGCTCCCTTGGGAACCGATTGTG 54.2 64.4

Os03g05290 aquaporin protein Os03g05290-F GCCACACACACACCACTCCATTTC 54.2 64.4

Os03g05290-R TTGCACTACTCCCTTCTGCTGCTC 54.2 64.4

Os03g06410 protein kinase domain containing protein Os03g06410-F GAGTGAAGTGCGGATTATGCGTCG 54.2 64.4

Os03g06410-R TTTGGAGGACGAGTAACAGCACCC 54.2 64.4

Os03g08230 sodium/calcium exchanger protein Os03g08230-F ACATTGCTCCCCTTTGGAAATGGC 50 62.7

Os03g08230-R TCCCCATAAACGCGGCAATACTCG 54.2 64.4

Os03g08560 plasma membrane ATPase Os03g08560-F CTGTTCCGGGCGACAGATTTTAGC 54.2 64.4

Os03g08560-R CCCAAGCAAAGGTTCACCTGGTTC 54.2 64.4

Os03g10640 calcium-transporting ATPase, plasma membrane-type Os03g10640-F AACGCCATTGTGCGAGTTGACG 54.5 62.1

Os03g10640-R ATAGCCAACGCGGGCATTGGTTTC 54.2 64.4

Os03g12390 STE_MEK_ste7_MAP2K.6 Os03g12390-F AGCCGCCGGTTAAATTAACTCTGC 50 62.7

Os03g12390-R ACACACGGGACAAAGCTGTTCGTC 54.2 64.4

Os03g14690 vacuolar ATP synthase 98 kDa subunit Os03g14690-F TTCCCTGGATGCTGATCCCAAAGC 54.2 64.4

Os03g14690-R TCCTTGATGCCTTCGCTCATGCTC 54.2 64.4

Os03g15570 STE_MEKK_ste11_MAP3K.12 Os03g15570-F TTCTCCCCCAAACCCAAAGCCAAG 54.2 64.4

Os03g15570-R TTCGTGATAAGTGCCAAGCGAGC 52.2 62.4

Os03g17310 calcium-transporting ATPase, endoplasmic reticulum-type Os03g17310-F ATTGCGTTGCGCCACCAATCTC 54.5 62.1

Os03g17310-R TCGCATGGAACCGAAACCCTAGAG 54.2 64.4

Os03g17700 CGMC_MAPKCGMC_2_ERK.2 Os03g17700-F AGCAGCAGCGATCACAAATTGTGC 50 62.7

Os03g17700-R TATTAAAAAGGGAAGGGGGCGGCG 54.2 64.4

Os03g18170 STE_MEKK_ste11_MAP3K.13 Os03g18170-F CTGCTGTCATTACCACGGGCAATC 54.2 64.4

Os03g18170-R CGATGTACTCGTACTCGTTGCTGC 54.2 64.4

Os03g20380 CAMK_KIN1/SNF1/Nim1_like.2 Os03g20380-F GTAGGCTGCTCCGATGAAACAACG 54.2 64.4

Os03g20380-R TAACACGGCGACAAGCTCATCCAC 54.2 64.4

Os03g20410 aquaporin SIP2-1 Os03g20410-F AACCCTGCATCTGCTTTTGCTTGG 50 62.7

Os03g20410-R GTGGTCGAATGTTGTGTGATCCCC 54.2 64.4

Os03g21890 potassium transporter Os03g21890-F ACGCCAGCTATATCCGTGTTCTCG 54.2 64.4

Os03g21890-R TCACGGTGGTCTTTGGACAAGGAG 54.2 64.4

Os03g22050 CAMK_KIN1/SNF1/Nim1_like.16 Os03g22050-F GGGTTTATGCACAGCAAGTGCTCC 54.2 64.4

Os03g22050-R TGAACTGCCACATCTAGCCCCATC 54.2 64.4


28

Os03g25070 CAMK_CAMK_like.18 Os03g25070-F TCCCGTTTCTTGATTTCCGGTCCC 54.2 64.4

Os03g25070-R AAAAATCCTTCCCCCTCCCCTTCC 54.2 64.4

Os03g27960 sodium/calcium exchanger protein Os03g27960-F CCAACCACCAAGGATGGGTTTTCC 54.2 64.4

Os03g27960-R AATCTCTCAGCCAAGGGCGTGATG 54.2 64.4

Os03g33570 calcineurin B-like protein 8 Os03g33570-F TGCTCGTGAATCGGATGCAAC 52.4 59.8

Os03g33570-R TCCTCCTGTATATCCGTTCCTGC 52.2 62.4

Os03g37830 potassium transporter Os03g37830-F TTCGCCCTCTTCACCATCGTCAAG 54.2 64.4

Os03g37830-R TACAGTGCAAACGTTCCACCGTCG 54.2 64.4

Os03g37840 potassium transporter Os03g37840-F TTACACGGCTACGCACCGTTCTTG 54.2 64.4

Os03g37840-R AGCCAGCACGCTATGCCTCAAAAG 54.2 64.4

Os03g37930 potassium transporter Os03g37930-F GTCATCAAGATCGTCTTCGTCGCC 54.2 64.4

Os03g37930-R AGAGTGCAAATGTCCCACCGTCAC 54.2 64.4

Os03g42020 calcium-transporting ATPase, plasma membrane-type Os03g42020-F AGGCGAGCATCGCATCATGAGAAG 54.2 64.4

Os03g42020-R CGAGTGTAGCTTTGGATGGCAGTG 54.2 64.4

Os03g42840 calcineurin B Os03g42840-F AGACCGTCTTTAGTGTGAGCGAGG 54.2 64.4

Os03g42840-R TGATCAGTCCGTCATCGATCACCG 54.2 64.4

Os03g43440 CAMK_KIN1/SNF1/Nim1_like.17 Os03g43440-F TGCCTTGTTGTTGGTGTCGC 50 59.4

Os03g43440-R TTCTTGGCCGCCTTGCTCTTG 57.1 61.8

Os03g43760 protein kinase domain containing protein Os03g43760-F ACACACATACCGGGTCGTGCATTC 54.2 64.4

Os03g43760-R AGTGGCACCAAACGCACAGAAAC 52.2 62.4

Os03g44440 cyclic nucleotide-gated ion channel Os03g44440-F ATCCGATCAACCATCCAGGAGCAC 54.2 64.4

Os03g44440-R TCACCAAGCCCACCTCACGAATAC 54.2 64.4

Os03g45370 sodium/calcium exchanger protein Os03g45370-F ACTGCCGTCCTACTCCAAGAACTC 54.2 64.4

Os03g45370-R ATCGCGGATAGGAGGCAATGAAGG 54.2 64.4

Os03g48270 CAMK_CAMK_like.21 Os03g48270-F TCCCTCATGAACCGCCTCAAGAAG 54.2 64.4

Os03g48270-R CGCGAACATGTCCTTGATCACCTC 54.2 64.4

Os03g48310 plasma membrane ATPase Os03g48310-F AGCCAAGGTGCTCCGAAATGGTAG 54.2 64.4

Os03g48310-R ACTGATGATGTCCCCAGGCACAAG 54.2 64.4

Os03g49640 STE_MEKK_ste11_MAP3K.14 Os03g49640-F TTAAGTCGTGGATGCGTGGTGCTC 54.2 64.4

Os03g49640-R AACGCTCCTTCATCACTGATGCCC 54.2 64.4

Os03g50550 STE_MEK_ste7_MAP2K.7 Os03g50550-F AGGGAGAAGAGGTTTAGCCAGATG 50 62.7

Os03g50550-R ATCTTGGAAGGGTACACGCGAC 54.5 62.1

Os03g52090 calcium-transporting ATPase 3, endoplasmic reticulum-type Os03g52090-F TACTTCCAGCAACGGAGCTTGTCC 54.2 64.4

Os03g52090-R CGGAACTTTGCATCCAACTCCCAC 54.2 64.4

Os03g54100 potassium channel protein Os03g54100-F ACCTGACCCTAGCACAATCTGCTC 54.2 64.4

Os03g54100-R AGCGGAAGCAAAGGAAACCGTTG 52.2 62.4


29

Os03g55100 cyclic nucleotide-gated ion channel 2 Os03g55100-F TGGAACGATATGGTGGGGATTCGG 54.2 64.4

Os03g55100-R CCACCTGCGATGTGAGAAGCAATG 54.2 64.4

Os03g55560 STE_MEKK_ste11_MAP3K.15 Os03g55560-F ACAGCAAAACCGACTCCCGAGAAG 54.2 64.4

Os03g55560-R TCACAGTGGCAATGGCGATGTGAG 54.2 64.4

Os03g57450 CAMK_CAMK_like.2 Os03g57450-F ACATTCGCGGCGTAATCCGAAG 54.5 62.1

Os03g57450-R AATCTTTCTCGGACACCACGCCTC 54.2 64.4

Os03g57510 CAMK_CAMK_like.23 Os03g57510-F TGCCCATGAAGCCCTATGTCATCC 54.2 64.4

Os03g57510-R AGGCTTGTCAGGAGCAACTCCATC 54.2 64.4

Os03g59390 CAMK_CAMK_like.24 Os03g59390-F TCATGCACCGCGACCTTAAACCAG 54.2 64.4

Os03g59390-R ATTTCAGTGAACCGCTCACCAGGC 54.2 64.4

Os03g60150 protein kinase domain containing protein Os03g60150-F TTCGCCTCAAGAAGGCAGGCAATG 54.2 64.4

Os03g60150-R AAGGGAAACCGTCTCAGGACATGC 54.2 64.4

Os03g63330 aspartokinase, chloroplast precursor Os03g63330-F TGCACAGGTTTTGCATCCCCAATC 50 62.7

Os03g63330-R ATAACAGTACCAGGTGCTCGACGG 54.2 64.4

Os03g64330 aquaporin protein Os03g64330-F AGCGGCAGCAATCCTAAGGACTAC 54.2 64.4

Os03g64330-R ACTGATGCACACCAACAGCAGC 54.5 62.1

Os04g02720 potassium channel KAT2 Os04g02720-F TCAATCTTCTCCGTCTCTGGCGAC 54.2 64.4

Os04g02720-R GCAAACAGTGTCACACAGAGGAGC 54.2 64.4

Os04g16450 aquaporin protein Os04g16450-F TCAATAAAACCGGCCAGTCCCACG 54.2 64.4

Os04g16450-R GCAAGCTCAACACGCTACTCACTC 54.2 64.4

Os04g32920 potassium transporter Os04g32920-F ACAGAGGGAATGTTTGCCGACCTG 54.2 64.4

Os04g32920-R TGCAGTTGAAGCTGATCTGCACG 52.2 62.4

Os04g35700 STE_MEKK_ste11_MAP3K.16 Os04g35700-F GGCAACTTTCGCACCCAAACATTG 50 62.7

Os04g35700-R TGAGCCCCCAGAAACAAACTCCAG 54.2 64.4

Os04g36740 potassium channel SKOR Os04g36740-F GCCGTTCGATCCAAGATGTCACAG 54.2 64.4

Os04g36740-R AACCTCTGAACAGCCCAACTCTCG 54.2 64.4

Os04g44060 aquaporin protein Os04g44060-F GCAGCGAGAAAGCTAAGCACACAC 54.2 64.4

Os04g44060-R ATGTCCTTCGCCATCTCCGATCAC 54.2 64.4

Os04g44570 aquaporin protein Os04g44570-F CCAGGTGCAGACGAACGGAAAAAG 54.2 64.4

Os04g44570-R AGCATGGTTGACAACGACCAAGC 52.2 62.4

Os04g46490 aquaporin protein Os04g46490-F GCAATGGCGAACATCTGTGCCAAC 54.2 64.4

Os04g46490-R AAGGTGGAGAAGAACTCGGCGAAG 54.2 64.4

Os04g47220 aquaporin protein Os04g47220-F TCAACAACTCCACCTCCAAGTGCG 54.2 64.4

Os04g47220-R AGTAGACGAGGGCGAAGATCATGC 54.2 64.4

Os04g47240 STE_MEKK_ste11_MAP3K.17 Os04g47240-F TCGCCGCATGTATACTCCTCTTGC 54.2 64.4

Os04g47240-R CAATGGCATCAGGACGCCAAGAAG 54.2 64.4


30

Os04g47300 CAMK_CAMK_like.26 Os04g47300-F TGGGAAAGAAGCCGACATCTGGAG 54.2 64.4

Os04g47300-R CCACGGGCTGGTACTGAAATCAAC 54.2 64.4

Os04g49510 CAMK_CAMK_like.27 Os04g49510-F AACCCCATCCAGTTCAGTTCCCTC 54.2 64.4

Os04g49510-R TGATCTAGCAAGCGAGAGAGCGAG 54.2 64.4

Os04g49570 glutamate receptor Os04g49570-F ATGCGAATCCCGACTCTGGACTTG 54.2 64.4

Os04g49570-R CGCATACCCATTGGATACCATGCC 54.2 64.4

Os04g51270 vacuolar ATPase G subunit Os04g51270-F ACGCAAACAGACGCCAAAGTGG 54.5 62.1

Os04g51270-R TTGCCGATTTTGCAGCCCTAGC 54.5 62.1

Os04g51610 calcium-transporting ATPase, plasma membrane-type Os04g51610-F TGACTGGATCACCGACCGAAAAGG 54.2 64.4

Os04g51610-R AATCTCATGCCAAGCTTCAGCCCC 54.2 64.4

Os04g51820 OsHKT1;1 - Na+ transporter Os04g51820-F GCCTGATGCCACTTGCAAAGATGC 54.2 64.4

Os04g51820-R ACGTGAGCACTCAAGCTTCTCCTC 54.2 64.4

Os04g51830 OsHKT1;4 - Na+ transporter Os04g51830-F AGAGATGCTTCTGTCACTGGCTCC 54.2 64.4

Os04g51830-R TTGCAGCCATCACAATGACATCCG 50 62.7

Os04g52120 potassium transporter Os04g52120-F ACATCGAGGACAGCGAGGAGTTTG 54.2 64.4

Os04g52120-R ACACCACAGCAAGAGTCTGAAGGG 54.2 64.4

Os04g52140 protein kinase domain containing protein Os04g52140-F CTGGAACACCAGAATGGATGGCAC 54.2 64.4

Os04g52140-R ACTTTTCGTTTGATGGCTCGCCAC 50 62.7

Os04g52390 potassium transporter Os04g52390-F TGAATTCCAGGAGCCGTGTTTGCG 54.2 64.4

Os04g52390-R GCAGGAAACGATTCCAGAAGCAGC 54.2 64.4

Os04g55040 vacuolar ATP synthase subunit D 1 Os04g55040-F AGGCTGGAGAACACCATCAGCTAC 54.2 64.4

Os04g55040-R TTGAGCCTGAAGAAGTCCTCACGC 54.2 64.4

Os04g55080 cyclic nucleotide-gated ion channel Os04g55080-F TCGGCAACATGCAGACCTACCTTC 54.2 64.4

Os04g55080-R ATGCATCCACTGCTCAGCATCACG 54.2 64.4

Os04g55940 sodium/calcium exchanger protein Os04g55940-F CCGCCAGTTTCTTCGTTCACGTTG 54.2 64.4

Os04g55940-R AGTGTCCGTGGCATCAATCATCGC 54.2 64.4

Os04g56160 plasma membrane ATPase Os04g56160-F ATCGAACGCCCTGGTCTTCTCTTG 54.2 64.4

Os04g56160-R AGGAATGTCGCAACCAGTTGTGC 52.2 62.4

Os04g56530 STE_MEKK_ste11_MAP3K.1 Os04g56530-F GCTTGCCTTGTTGGCCTTATTCCG 54.2 64.4

Os04g56530-R TTCCTTTGCACGATCTGGCACG 54.5 62.1

Os04g58620 potasium efflux antiporter protein Os04g58620-F AAAGCTGGTGCAACAGCGGTTGTC 54.2 64.4

Os04g58620-R AGCAGCCAACTGGAGACTTGGTTC 54.2 64.4

Os04g59310 phospholipase C Os04g59310-F ATGTTCACGTCCAAGAGGGGCAAG 54.2 64.4

Os04g59310-R TCGTTCCCATACTGGGTTTCCAGG 54.2 64.4

Os05g01560 vacuolar ATP synthase Os05g01560-F GAAGCTCTTGCTCTGTATGGCCTG 54.2 64.4

Os05g01560-R TTCCAAAACCCTCTAATCCGCTCG 50 62.7


31

Os05g02940 calcium-transporting ATPase 2, endoplasmic reticulum-type Os05g02940-F TGGTGTTGCTTGTTGCACTCCC 54.5 62.1

Os05g02940-R TTTACTCGCCCTTCTGCTTCCTGG 54.2 64.4

Os05g04550 CAMK_KIN1/SNF1/Nim1_like.3 Os05g04550-F GCTGTAAACCTTGCTGCCCACTTG 54.2 64.4

Os05g04550-R TTGAAGGGGGAAGAAGGTGTCTGG 54.2 64.4

Os05g05160 CGMC_MAPKCGMC_2.1 Os05g05160-F TTTCTTGGCCGCATCCTCCTCTTC 54.2 64.4

Os05g05160-R CGCGCACAAGGCAAAAAGAAAGG 52.2 62.4

Os05g05590 transporter, monovalent cation:proton antiporter-2 family Os05g05590-F ATGAATCCATCACCGCGCTTGTC 52.2 62.4

Os05g05590-R ATGTGCGAGTTCTTCCCACCACTG 54.2 64.4

Os05g06480 inorganic H+ pyrophosphatase Os05g06480-F ACTCGCTCGTCTTCTCCTCCATTG 54.2 64.4

Os05g06480-R TGAACGAAGCGCAAAGCTAAGCTC 50 62.7

Os05g11560 aquaporin protein Os05g11560-F GGCAGCAGAGATCATCAAGCAAGG 54.2 64.4

Os05g11560-R GCGAATTGGACAGAGAACATGGGG 54.2 64.4

Os05g11790 CAMK_KIN1/SNF1/Nim1_like.19 Os05g11790-F ACCCATTAACGACATGGGCTCTCC 54.2 64.4

Os05g11790-R GGATTAGTGTATGTTCACGGCGGC 54.2 64.4

Os05g14240 aquaporin protein Os05g14240-F TTTGCTGCCCTTTTCATCCCCTCG 54.2 64.4

Os05g14240-R ATCCCAAAGCTCTCGCCTTTCCTC 54.2 64.4

Os05g25550 E1-E2 ATPase domain containing protein Os05g25550-F AAGTTCGCCATCCGCTACTTCCTC 54.2 64.4

Os05g25550-R AACGCCGTCTTGTTGTCGAAGG 54.5 62.1

Os05g26820 CAMK_KIN1/SNF1/Nim1_like.20 Os05g26820-F ACCCTGCAAACCTGCATTGCTG 54.5 62.1

Os05g26820-R AAGAAACCCGAATCCGACTCCCAC 54.2 64.4

Os05g26940 CAMK_KIN1/SNF1/Nim1_like.23 Os05g26940-F ATGGGAGGCTGAATTGGAGCAAGC 54.2 64.4

Os05g26940-R GGCGAACCCGATTATGTCGAAAGC 54.2 64.4

Os05g35410 potassium channel AKT2/3 Os05g35410-F GCACTGCATATAGCGGCATCGAAG 54.2 64.4

Os05g35410-R TGCACGCTTGCTTGAGGAGAAC 54.5 62.1

Os05g39090 CAMK_CAMK_like.29 Os05g39090-F TGCTCTGCTCTGCTCTACTACAGC 54.2 64.4

Os05g39090-R TTTGTTCCGCATTGGGCTCTGC 54.5 62.1

Os05g39870 CAMK_KIN1/SNF1/Nim1_like.24 Os05g39870-F TCAAAGGCGAGGCTTACAATGCAG 50 62.7

Os05g39870-R GGCTCTAGCAATTTGGCACAGACC 54.2 64.4

Os05g39890 CBL-interacting serine/threonine-protein kinase 15 Os05g39890-F AGGTATGGTCAAAGAGAGGCACGG 54.2 64.4

Os05g39890-R GGCCAGTTCCTTCAGCTTCACAAG 54.2 64.4

Os05g39900 CBL-interacting serine/threonine-protein kinase 15 Os05g39900-F TGCCAAGAGTGGCAAGCTTTTCC 52.2 62.4

Os05g39900-R TTTGCGATCTTCTTTCTCGCCGAC 50 62.7

Os05g40230 vacuolar ATP synthase subunit E Os05g40230-F GTGTGTCGAAGATGAACGATGCCG 54.2 64.4

Os05g40230-R AGGATGAACCTGACCATCTGCTGG 54.2 64.4

Os05g41090 CAMK_CAMK_like_CG18020d.1 Os05g41090-F AGCTGTTCTGTTGTCACCTCGTCC 54.2 64.4

Os05g41090-R GTGAAAATGTGAAATCCGCACCGC 50 62.7


32

Os05g41270 CAMK_CAMK_like.4 Os05g41270-F TTGGCAGGCTCAAGCAGTTCAGAG 54.2 64.4

Os05g41270-R ATCCGGCGATAACCCTTAGAGCAG 54.2 64.4

Os05g41580 calcium-transporting ATPase, plasma membrane-type Os05g41580-F CAGCAAATGTGGACTGCTTGGAGG 54.2 64.4

Os05g41580-R GCAGAACCAGCTCAAACTGTACGC 54.2 64.4

Os05g42250 cyclic nucleotide-gated ion channel 2 Os05g42250-F TGACCCTCAGCACGTTTGGGAATC 54.2 64.4

Os05g42250-R TTGAACACGATCTCCACCCACTCC 54.2 64.4

Os05g43840 CAMK_KIN1/SNF1/Nim1_like.25 Os05g43840-F TGCAAGCATGAACACCTGAGAGGC 54.2 64.4

Os05g43840-R ACAGCACCAAAACATGGACACTGG 50 62.7

Os05g45810 calcineurin B Os05g45810-F CGCGAGCTGTACAACAAGATGAGC 54.2 64.4

Os05g45810-R GCAAGCTGAAACTCCTCCTTGTGG 54.2 64.4

Os05g45950 outer mitochondrial membrane porin Os05g45950-F TACCACACGGACCAGAAGTTCACG 54.2 64.4

Os05g45950-R TGCCAGCAACAGTGATTGCAGC 54.5 62.1

Os05g46750 STE_MEKK_ste11_MAP3K.18 Os05g46750-F AGCAGCCAATCCGAACTTCCCATC 54.2 64.4

Os05g46750-R TTCAGAAACTTCGCTGCGCGTC 54.5 62.1

Os05g46760 STE_MEKK_ste11_MAP3K.19 Os05g46760-F TTCGAGCTTCCCCACATTGACGTG 54.2 64.4

Os05g46760-R ATGTGCTGAGCATTGGCTTCTCCC 54.2 64.4

Os05g49140 CGMC_MAPKCMGC_2.8 Os05g49140-F TGGCTTTGTGTTGTGCCCATCC 54.5 62.1

Os05g49140-R AGATGCGACCAATTCCCTTGCCAG 54.2 64.4

Os05g50120 CGMC_MAPKCMGC_2.3 Os05g50120-F GCCTTGCCCGAGTGTCATTTAACG 54.2 64.4

Os05g50120-R TGGGGCACGATACCATCTAGTTGC 54.2 64.4

Os05g50190 protein kinase domain containing protein Os05g50190-F ACATGGCTCCTGAGGTTCTCAACG 54.2 64.4

Os05g50190-R ACAGCAGACGTGACCTCTGAGAAG 54.2 64.4

Os05g50560 CGMC_MAPKCMGC_2.9 Os05g50560-F CACCACGAATCCACACACACCATC 54.2 64.4

Os05g50560-R TCGAAATCAAAAGGAGGGGAGCGG 54.2 64.4

Os05g50810 CAMK_CAMK_like.5 Os05g50810-F TACAAGAGCAGTTCGTATCGGCGG 54.2 64.4

Os05g50810-R AAACGCACCACACCCACTTCAGAG 54.2 64.4

Os05g51530 vacuolar ATP synthase subunit C Os05g51530-F ACCTCACCAGGTTTGTGTGGGATG 54.2 64.4

Os05g51530-R ATGCTGCCGACAATCTCCTTGAGC 54.2 64.4

Os05g51610 sodium/calcium exchanger protein Os05g51610-F TCAGCGAGCGAGGAACAACTAGAG 54.2 64.4

Os05g51610-R TTCGACTCCATCATCATCCGCGAC 54.2 64.4

Os06g05520 OsMKK1 - putative MAPKK based on amino acid sequence homology

Os06g05520-F ACTTTGCCCTGTGTACTGAGCTGG 54.2 64.4

Os06g05520-R CGGCCAAACAAAACTTCAATGCCC 50 62.7

Os06g06090 CGMC_MAPKCMGC_2_ERK.12 Os06g06090-F CAAGCATTGTCAGAGGAGCACTGC 54.2 64.4

Os06g06090-R TAGGTTGCTGGGCTTCAAGTCTCG 54.2 64.4

Os06g06130 glutamate receptor Os06g06130-F GAACACCTGCCGTACAACTTCCAC 54.2 64.4


33

Os06g06130-R TCGCCGACAAGGATGTCATAGCTC 54.2 64.4

Os06g08080 inorganic H+ pyrophosphatase Os06g08080-F ACCAACTGGGGCTTGTTCTTCTGC 54.2 64.4

Os06g08080-R ACCAATGATCAGACCAGCCCACAG 54.2 64.4

Os06g08280 protein kinase domain containing protein Os06g08280-F TGTTGTTTTGGCGAGTCCTGGAGG 54.2 64.4

Os06g08280-R ACAACCAAACTCACCACACCGCAC 54.2 64.4

Os06g08310 plasma membrane ATPase Os06g08310-F ATGACCATCTCCAAGGATCGCGTC 54.2 64.4

Os06g08310-R TCGTTGAGCTTCCAACAATCGGGC 54.2 64.4

Os06g08850 cyclic nucleotide-gated ion channel 14 Os06g08850-F TGAGCATGACGACTAAGCGGAGAG 54.2 64.4

Os06g08850-R GCAGCGCAAACGGTGATTTCACAG 54.2 64.4

Os06g08880 glutamate receptor 2.7 precursor Os06g08880-F ACTGCCAAGGCTACGCCATAGTTG 54.2 64.4

Os06g08880-R CAGACGAATCCAAATCCACCGCTC 54.2 64.4

Os06g08900 glutamate receptor 2.6 precursor Os06g08900-F TTCACGATGCCATACACGTCGTCC 54.2 64.4

Os06g08900-R AAGAAGAAGCCGATGGTGGCAAGC 54.2 64.4

Os06g08910 glutamate receptor 2.8 precursor Os06g08910-F TGGCCCTGTGCCTACAAATTCCAC 54.2 64.4

Os06g08910-R TTTCCAGAGCACGCTCAGCATGAC 54.2 64.4

Os06g08930 glutamate receptor 2.8 precursor Os06g08930-F CAGCAACCCTGAACCTGATCAAGC 54.2 64.4

Os06g08930-R TTGCCTGCATCAGTTGGGCATC 54.5 62.1

Os06g09090 glutamate receptor 2.8 precursor Os06g09090-F TTCCCACTCAGCAACTAGCCATGC 54.2 64.4

Os06g09090-R ACTGTCTAATCCAACACGGCCTGC 54.2 64.4

Os06g09120 glutamate receptor 2.8 precursor Os06g09120-F TGCCTGCAAATTCCAGCCAGAC 54.5 62.1

Os06g09120-R GCTCCGTGTGACAGTGTCAACATC 54.2 64.4

Os06g09130 glutamate receptor 2.8 precursor Os06g09130-F TCAACCACAACCACCGCAGATTG 52.2 62.4

Os06g09130-R AATCTGGAGGGTATCAGCAGGCAC 54.2 64.4

Os06g09180 OsMKK5 - putative MAPKK based on amino acid sequence homology

Os06g09180-F TGCCAATTCCATCCGGTCATCGTG 54.2 64.4

Os06g09180-R GGGCAGCAGCAATGGATGAAAACG 54.2 64.4

Os06g09190 mitogen-activated protein kinase kinase 5 Os06g09190-F TGCAGATGAATCCGGCGAAG 50 59.4

Os06g09190-R TGACGAACGGGTGATCCATGAG 54.5 62.1

Os06g10580 cyclic nucleotide-gated ion channel Os06g10580-F TTGGGTTCCTTAAGAAGGTCCGGC 54.2 64.4

Os06g10580-R TTGCATCCCAGGTTGCATTGTCC 52.2 62.4

Os06g12310 aquaporin protein Os06g12310-F GCAGCACAGCTTGATCGTTCTTCC 54.2 64.4

Os06g12310-R AGGACAACGAATCAACGGACCAGC 54.2 64.4

Os06g12590 protein kinase Os06g12590-F AAATGGAGGTCAACACCAGGCGAC 54.2 64.4

Os06g12590-R GAAGGCATGAAAGACCAAGGCACC 54.2 64.4

Os06g13730 glutamate receptor precursor Os06g13730-F ATTCGCTTTCCATCACGACTCCCC 54.2 64.4

Os06g13730-R TTCATCAGATCGGATTGCCTCCGC 54.2 64.4


34

Os06g14030 potassium channel SKOR Os06g14030-F TCTGAGAGTAGCGAATACGGCAGC 54.2 64.4

Os06g14030-R TGCTTCCCGATGTGGAACGTGATG 54.2 64.4

Os06g14310 potassium channel AKT1 Os06g14310-F TTGTCACAGACCTGGTGGTCGATG 54.2 64.4

Os06g14310-R TGTCACGGTATGCGACGAAGAAGG 54.2 64.4

Os06g15910 potassium transporter Os06g15910-F TGTCTCCGTGTTCTCAGCGGTTTC 54.2 64.4

Os06g15910-R TGCAAGGCGAATAAGCACACCAG 52.2 62.4

Os06g21360 transporter, monovalent cation:proton antiporter-2 family Os06g21360-F TCGAGGAGAACAAGTGGGTCAACG 54.2 64.4

Os06g21360-R GCACCTACAACACAACCGATGAGG 54.2 64.4

Os06g22960 aquaporin protein Os06g22960-F CGGCAACACGCATAAAAATGGTGG 50 62.7

Os06g22960-R TCGCAACAAAAGCCTTCTTCCCC 52.2 62.4

Os06g26340 CGMC_MAPKCMGC_2.10 Os06g26340-F CAACTCAGATTCGGAGCTACTGCC 54.2 64.4

Os06g26340-R TTCCCCTTCTTCTCCTCCTCATGC 54.2 64.4

Os06g27890 STE_MEK_ste7_MAP2K.8 Os06g27890-F AGCAAATAGCAGAGATGCTTGCCG 50 62.7

Os06g27890-R AATGCCAAATCCCATCAGTGCCG 52.2 62.4

Os06g33570 cyclic nucleotide-gated ion channel 1 Os06g33570-F AGCATTCCATCCCGTGTTCCCATC 54.2 64.4

Os06g33570-R AATGTGATCTTGCTCCGTGCCTGG 54.2 64.4

Os06g33600 cyclic nucleotide-gated ion channel 1 Os06g33600-F TGTCTTTGCTGTTCTACCCCTCCC 54.2 64.4

Os06g33600-R GGCTTTTGCAACCTCTGAGCTTCG 54.2 64.4

Os06g33610 cyclic nucleotide-gated ion channel 1 Os06g33610-F AGTTTCCAGGCTCCCTAGCTCAAC 54.2 64.4

Os06g33610-R AGGGCAAAAGCTTCGACTTCGGAC 54.2 64.4

Os06g35160 CAMK_KIN1/SNF1/Nim1_like.26 Os06g35160-F ATCCCCCCTAACAGCAGCAGAATC 54.2 64.4

Os06g35160-R ATGGATCGGAACATGCACGCAC 54.5 62.1

Os06g35930 aquaporin protein Os06g35930-F AAGGAGGATGACCACCAAAACCCG 54.2 64.4

Os06g35930-R TCTGGAGGAAGGTGAACGACATGG 54.2 64.4

Os06g37180 ATP synthase Os06g37180-F TGACGGTGAAAAGGCTGTTGTGC 52.2 62.4

Os06g37180-R TTCAGGACCTCTCCTGTGAACTGC 54.2 64.4

Os06g40370 CAMK_KIN1/SNF1/Nim1_like.27 Os06g40370-F ACGTTCGCCAAGGTCAAGTTCG 54.5 62.1

Os06g40370-R TGGTTGAGGATGGTGTCCTTGTCG 54.2 64.4

Os06g42030 potassium transporter Os06g42030-F TGCAGACTTGCAGAGCCATGAAAC 50 62.7

Os06g42030-R TCTCCTGGCAATCCGCATACTGAC 54.2 64.4

Os06g43030 protein kinase domain containing protein Os06g43030-F TCGATCCGCCAAGAAAAGCAGC 54.5 62.1

Os06g43030-R AGCAAAGCTCAGATCCCCATCTCC 54.2 64.4

Os06g43660 inorganic H+ pyrophosphatase Os06g43660-F TTACACAAGCAACGCCTACAGCCC 54.2 64.4

Os06g43660-R TCCCAGAGCAAGGCCAAAGATGAC 54.2 64.4

Os06g45120 ATP synthase Os06g45120-F GCCTGTTTCATCAAAGCTTGCTGC 50 62.7

Os06g45120-R GTGCATCAAGTACACGCTGTCCTG 54.2 64.4


35

Os06g45940 potassium transporter Os06g45940-F TCTTCAGGAAGGCTGGCAAGGATG 54.2 64.4

Os06g45940-R GGCTTCAGCACCTGTAAGGCAAAG 54.2 64.4

Os06g46670 glutamate receptor Os06g46670-F TCGATGAAGCGGAGACCAAGTGAG 54.2 64.4

Os06g46670-R TCTACGGTTAAGCCACTGACTGCG 54.2 64.4

Os06g48590 CGMC_MAPKCMGC_2_SLT2y_ERK.2 Os06g48590-F TGGCAGGAGATGCTCCACTATCAC 54.2 64.4

Os06g48590-R AGCACGTTGCCTTCACATGTTCG 52.2 62.4

Os06g48800 OsHKT2;4 - Na+ transporter Os06g48800-F TGATTGTTCCCTTGTTGCGCCAG 52.2 62.4

Os06g48800-R GCTTTCCGGGCCGATTTGTTTGTG 54.2 64.4

Os06g48810 OsHKT2;1 - Na+ transporter Os06g48810-F TCTCGGTCACAGTCTCCTCGTTTG 54.2 64.4

Os06g48810-R TGCCATGTTCTCATTCGTCGGCAC 54.2 64.4

Os06g49430 CGMC_MAPKCMGC_2.11 Os06g49430-F CCAAGCAGAATGCTGCACTGCAAG 54.2 64.4

Os06g49430-R AAGATGCCAATCATCTCGGGCTGG 54.2 64.4

Os06g50030 CAMK_CAMK_like.30 Os06g50030-F CGCTGTTCAAGCACTGACTCATCC 54.2 64.4

Os06g50030-R AAGAGGTGTAGCGCGGAGGTATTG 54.2 64.4

Os07g01310 glutamate receptor Os07g01310-F TTCTCAACGGGACATACCTGAGCG 54.2 64.4

Os07g01310-R TGCAGACCTTGGATGATGCCGATG 54.2 64.4

Os07g01810 potassium channel protein Os07g01810-F TTCGTGCTGTACAAGCTCAAGGAG 50 62.7

Os07g01810-R GTTGTCGAACTCGTCGAGGAACTC 54.2 64.4

Os07g02780 STE_MEKK_ste11_MAP3K.20 Os07g02780-F AGCTACCAATCGGCAAACTGGAGC 54.2 64.4

Os07g02780-R CTCGGCAGATTTCGCATCATCAGG 54.2 64.4

Os07g05620 CAMK_KIN1/SNF1/Nim1_like.28 Os07g05620-F TCGCCATCAAGATCCTCGACAAGG 54.2 64.4

Os07g05620-R TCGCGCTTTATCTGGGCGATCATC 54.2 64.4

Os07g06740 CAMK_CAMK_like.31 Os07g06740-F ATCAGCCGGACACACAAGAAAGCC 54.2 64.4

Os07g06740-R TTGGAGGGTCCACGGATGTTGAAC 54.2 64.4

Os07g07910 potassium channel AKT1 Os07g07910-F ATGGCCGCACAGCTCTGCATATAG 54.2 64.4

Os07g07910-R GAAGCCTGACACACTGCTCATTCC 54.2 64.4

Os07g09340 plasma membrane ATPase Os07g09340-F GCCGAAATCGCAAGGTTGAGAGAG 54.2 64.4

Os07g09340-R TTCTGCTTCACCACCGACTCCATC 54.2 64.4

Os07g20544 aspartokinase, chloroplast precursor Os07g20544-F TCACAAGGGGCATCCAAGGTGAAC 54.2 64.4

Os07g20544-R AATGCTGAGTGGAGGGCTTGAACG 54.2 64.4

Os07g22710 CAMK_CAMK_like.32 Os07g22710-F TTTGACCCCAATTCCCAACACCCC 54.2 64.4

Os07g22710-R TATTACCATCAATGTGGCGGCGGC 54.2 64.4

Os07g25680 protein kinase domain containing protein Os07g25680-F ACGCCCAGCATTCACAGAGATAGC 54.2 64.4

Os07g25680-R TCCCACCTGCGCCGTTTATTTG 54.5 62.1

Os07g25710 myb-like DNA-binding domain containing protein Os07g25710-F TTGCCTTTTCACGGGGAACAGAAC 50 62.7

Os07g25710-R GCCGTGTTTGGGGTACTGTACAAC 54.2 64.4


36

Os07g26630 aquaporin protein Os07g26630-F ACCTCTCTCAAGCAGCTTAGCTCC 54.2 64.4

Os07g26630-R TTGCCCATTGACCTCGTTGAGGAC 54.2 64.4

Os07g26640 aquaporin protein Os07g26640-F CAAGGCATGGAGTGATCAGTGG 54.5 62.1

Os07g26640-R AGGATGACCTGGTGGTACAGC 57.1 61.8

Os07g26690 aquaporin protein Os07g26690-F TTCGCCGCTGGTCGTTTTGTTTC 52.2 62.4

Os07g26690-R TTCACACACACGACAAGCAGGAGG 54.2 64.4

Os07g32530 potassium transporter Os07g32530-F ACCGCCACCAGGAAATTACCGTAG 54.2 64.4

Os07g32530-R ACACCTAGTGTCTGAACAGCCAGC 54.2 64.4

Os07g33110 CAMK_CAMK_like.33 Os07g33110-F AACATGGACACAGACAACAGCGGG 54.2 64.4

Os07g33110-R TTGCCGTCTACATCAGCAGCTTCC 54.2 64.4

Os07g33790 glutamate receptor 3.4 precursor Os07g33790-F CGCGCACAGAGAGAACACAGTTAG 54.2 64.4

Os07g33790-R ACAAACAGCCAGACAAGCAGCAC 52.2 62.4

Os07g36470 vacuolar ATP synthase subunit H Os07g36470-F GGCTTTTCCTTGGTGCGAAGTACG 54.2 64.4

Os07g36470-R AATGGCCTGGTGTTTCTCTGGCTC 54.2 64.4

Os07g38120 CAMK_CAMK_like.34 Os07g38120-F TTGGGCACCAAATGGCTGATGCAG 54.2 64.4

Os07g38120-R TTTCCATCGACATCCGCAGCATCC 54.2 64.4

Os07g42770 CAMK_CAMK_like.35 Os07g42770-F CCGACCGAAGATAACAGCTTGTGC 54.2 64.4

Os07g42770-R GGCAGGCGACAACAAACTGAACTC 54.2 64.4

Os07g43900 protein kinase domain containing protein Os07g43900-F GCGCACGATCACCATTGGCATTTG 54.2 64.4

Os07g43900-R AACACCACGCGCAGTAGCAATC 54.5 62.1

Os07g44290 CAMK_KIN1/SNF1/Nim1_like.29 Os07g44290-F ATGTGGCAGCCCTAACTACATCGC 54.2 64.4

Os07g44290-R ACCCCACAAGACCATATGTCGGAC 54.2 64.4

Os07g44710 CAMK_CAMK_like.36 Os07g44710-F TCCATCTTCCAGCTTCCAGGCTTC 54.2 64.4

Os07g44710-R AGCTCAGGCAGAGCAAAAATGGC 52.2 62.4

Os07g47100 transporter, monovalent cation:proton antiporter-2 family Os07g47100-F GCACATTGCAGGTCCTCAATCAGG 54.2 64.4

Os07g47100-R GCATCGTTCACAACACCTTCACCG 54.2 64.4

Os07g47350 potassium transporter Os07g47350-F TTTAAAAGACCCTGGCCCAGGAGG 54.2 64.4

Os07g47350-R TGCAGTGCAAACAGGCCAACAAG 52.2 62.4

Os07g48090 CAMK_KIN1/SNF1/Nim1_like.30 Os07g48090-F GTTCTCGCTATCATCGTGTCGTGC 54.2 64.4

Os07g48090-R ACAGTAGCCTCTGAACGGTGTTCC 54.2 64.4

Os07g48100 CAMK_KIN1/SNF1/Nim1_like.31 Os07g48100-F TGAGGGAATTGGCAGGGTAGCAAG 54.2 64.4

Os07g48100-R TAGTGCGAGAGCACATGGAGCAAG 54.2 64.4

Os07g48130 potassium transporter Os07g48130-F CACTTGATGCAGCCTGTTCGACTC 54.2 64.4

Os07g48130-R CCACAAAAGATCCCCGTTCAAGCC 54.2 64.4

Os07g48760 CAMK_KIN1/SNF1/Nim1_like.32 Os07g48760-F TCTACCAAACGCTCTCGACGCAAC 54.2 64.4

Os07g48760-R GCAGCAAACCTCATTCCGCTTAGC 54.2 64.4


37

Os08g01310 phospholipase C Os08g01310-F TGGCTGTGCCACTCATTTCAAGGG 54.2 64.4

Os08g01310-R TTGATGGCAGGTTGAAAGGCTGTG 50 62.7

Os08g05590 aquaporin protein Os08g05590-F CTGGTGCTTCTCATTTGGTGCTCG 54.2 64.4

Os08g05590-R ATCCTGTTGCTCTGATCACCTGCC 54.2 64.4

Os08g05600 aquaporin protein Os08g05600-F GAACATTCTTGTGGCAGGGCCATC 54.2 64.4

Os08g05600-R CCAATTGTACGCGCCGGATTCATC 54.2 64.4

Os08g06060 CGMC_MAPKCMGC_2_ERK.13 Os08g06060-F AACGGAAGCAGATCACACTGGCAG 54.2 64.4

Os08g06060-R TGTTGGCTCATGTCTTCCCCAAGG 54.2 64.4

Os08g10550 potassium transporter Os08g10550-F TTTGCTTCGGTGGAGAAGCTCTGG 54.2 64.4

Os08g10550-R TGAAAGCGACCTTTGCTCGAACC 52.2 62.4

Os08g12750 serine/threonine-protein kinase HT1 Os08g12750-F GTGTTGCATCTCCCAGCCAATGAC 54.2 64.4

Os08g12750-R TCTCCAGGTTGCCTTTTTCCACCC 54.2 64.4

Os08g25390 bifunctional aspartokinase/homoserine dehydrogenase, chloroplast precursor

Os08g25390-F AGTTGCACGCGGATGTCAGTAACC 54.2 64.4

Os08g25390-R TGCCCAGCTATGCAAATTGCACG 52.2 62.4

Os08g34240 CAMK_KIN1/SNF1/Nim1_like.33 Os08g34240-F GTGTCACAGTAGCCGATGCTGTTC 54.2 64.4

Os08g34240-R TGCACGTCGCTATCGAATCAAGC 52.2 62.4

Os08g36340 potassium transporter Os08g36340-F TCCGTCAGTAAACCTGCTGTGGAG 54.2 64.4

Os08g36340-R CAAGAACAGCCCAACGAGAATCGC 54.2 64.4

Os08g39950 potassium transporter Os08g39950-F TACATCGTGCTTGCTGGGACTTGC 54.2 64.4

Os08g39950-R AGCTGACAGAACTGAGATGGCTGG 54.2 64.4

Os08g40530 calcium-transporting ATPase 9, plasma membrane-type Os08g40530-F GCAACTGAACCACCGACGGATAAC 54.2 64.4

Os08g40530-R AAGAGGTTCCCTTCGACCAACAGG 54.2 64.4

Os08g42750 CAMK_CAMK_like.37 Os08g42750-F TTCTGGGGAGACAACGACGAGAAG 54.2 64.4

Os08g42750-R ATCAAGCATCCTCCTGACGAGGTC 54.2 64.4

Os08g43690 transporter, monovalent cation:proton antiporter-2 family Os08g43690-F TTCGCCAAATCAGAAGGGGTGTGG 54.2 64.4

Os08g43690-R AGGGTGCTGATCAAGTTGCCGATG 54.2 64.4

Os09g02214 Citrate transporter protein Os09g02214-F GTCCCTCACGAGTGAAGCAAATGG 54.2 64.4

Os09g02214-R GCTCTGATGACAGCAAGCTGGAAG 54.2 64.4

Os09g02729 phospholipase C Os09g02729-F TACCTCCATTGTGAAGGGACTGCC 54.2 64.4

Os09g02729-R TGGTTGTAGAACGTGACCCTCTCG 54.2 64.4

Os09g03680 ankyrin Os09g03680-F TTCGTCCTTCATCAGCAACCCAGG 54.2 64.4

Os09g03680-R CGACCACGATTAACACGAGTTGCC 54.2 64.4

Os09g11450 transporter, monovalent cation:proton antiporter-2 family Os09g11450-F ACCAGAGACGCGGATTCTATGACC 54.2 64.4

Os09g11450-R TATGTGGCTGTCAGGTGGCAACTC 54.2 64.4

Os09g12290 bifunctional aspartokinase/homoserine dehydrogenase, chloroplast precursor

Os09g12290-F TTAACCTTGAGCGTCCTGCCTTCC 54.2 64.4


38

Os09g12290-R CGGGCACAATCAATACGCCTCATC 54.2 64.4

Os09g12790 potassium channel protein Os09g12790-F ATCCGATCCCCAATCTGCTTCTCC 54.2 64.4

Os09g12790-R GAATCCGGCGGTTTCTTCGAATCC 54.2 64.4

Os09g19734 isochorismate synthase 1, chloroplast precursor Os09g19734-F TCAACAATTCCGGCAAGGCCAG 54.5 62.1

Os09g19734-R TCTGCTGAGATGGTCCACACAGAC 54.2 64.4

Os09g21000 potassium transporter Os09g21000-F ACCTTGAAGGTCCCTCACTCGAAC 54.2 64.4

Os09g21000-R TCTTGCAAAGCCCCAATCACACC 52.2 62.4

Os09g21510 STE_MEKK_ste11_MAP3K.2 Os09g21510-F AAGGTCTGGTCCAGGATTGCACAC 54.2 64.4

Os09g21510-R AAAAACCGCAGTAACCTCCGCC 54.5 62.1

Os09g25090 CAMK_KIN1/SNF1/Nim1_like.34 Os09g25090-F GCGCGACGTGAAATCTGTAGGATG 54.2 64.4

Os09g25090-R GCCTGTACATAACCGCGAGCAAAG 54.2 64.4

Os09g25100 CAMK_KIN1/SNF1/Nim1_like.35 Os09g25100-F TCCGTTGCTAATCCATTGGGGTGC 54.2 64.4

Os09g25100-R ACAAACGAGCGAGCCAGACATCAC 54.2 64.4

Os09g25960 glutamate receptor Os09g25960-F TGCAAAGCACTGCAAAGGGTACAC 50 62.7

Os09g25960-R CAGTGGGGATCGTTTGGGAAATGC 54.2 64.4

Os09g25980 glutamate receptor 2.6 precursor Os09g25980-F ACGCGGCACACACAAACTACAG 54.5 62.1

Os09g25980-R ATCAAGAGCTGCTGATGCAGCCTG 54.2 64.4

Os09g25990 glutamate receptor Os09g25990-F GCAAAGGGTACACAATGGTCGGAC 54.2 64.4

Os09g25990-R TAGGCGGAAAGCCAAAGCCTTCAG 54.2 64.4

Os09g26000 glutamate receptor Os09g26000-F TCAACCTCAGCCCTTTTGATCGCC 54.2 64.4

Os09g26000-R TGTGCTCTGGTCCATATCTGCTCC 54.2 64.4

Os09g26144 glutamate receptor Os09g26144-F GAATGGCAGAGGCCAGTAACAACG 54.2 64.4

Os09g26144-R TTTCGCTAAGGACATGGAGCACCG 54.2 64.4

Os09g26160 glutamate receptor Os09g26160-F TGCAGCCATTGCTCACTTCGCAAG 54.2 64.4

Os09g26160-R ATCATCAGCAAGAGGACGACGACG 54.2 64.4

Os09g27580 potassium transporter Os09g27580-F GGGAATGCTTTCGGTGTTGTGGTC 54.2 64.4

Os09g27580-R GAAGTACAGCCCAGCAAGCACAAG 54.2 64.4

Os09g30446 transporter, monovalent cation:proton antiporter-2 family Os09g30446-F TCTTCCTGAAGGACATGGCGAGAC 54.2 64.4

Os09g30446-R AGGGTAGCCAGCATTGTTCCCATC 54.2 64.4

Os09g31160 glutamate receptor precursor Os09g31160-F AGCTCTTCCTCTCCATCAACTGCC 54.2 64.4

Os09g31160-R ATCCGCTCTTTGTGAACTCCTGCC 54.2 64.4

Os09g33910 CAMK_CAMK_like.39 Os09g33910-F ATGCTTGACCCCGATCCTTCTACC 54.2 64.4

Os09g33910-R AGCCAAGGATGCTCAAGGACTTCC 54.2 64.4

Os09g36930 aquaporin protein Os09g36930-F ACCGTGATCGGGTACAAGAACCAG 54.2 64.4

Os09g36930-R ACGAATATGGTGGCACCGAACGAC 54.2 64.4

Os09g37230 protein kinase family protein Os09g37230-F ACAACAGAGCATTCACCCTCCTCC 54.2 64.4


39

Os09g37230-R CATGGTCTTGTCCTTGGGACTTGC 54.2 64.4

Os09g37300 transporter, monovalent cation:proton antiporter-2 family Os09g37300-F AAGGGCCACGTCAACATGATCGAC 54.2 64.4

Os09g37300-R TGCTTATGATGCTGCCGACGAC 54.5 62.1

Os09g38580 cyclic nucleotide-gated ion channel Os09g38580-F TGATTTGGGGCGGGAAGAAGCATC 54.2 64.4

Os09g38580-R TCTTGCTGTGTCAACTGAGGAGGC 54.2 64.4

Os09g38960 potassium transporter Os09g38960-F GCTCCCACGCGAATTCCTAACATC 54.2 64.4

Os09g38960-R ACACATCACCCATACGCTGGCTTG 54.2 64.4

Os10g04000 STE_MEKK_ste11_MAP3K.22 Os10g04000-F AACAAGGAAACCGTCAAGGCGAC 52.2 62.4

Os10g04000-R ACGACGACTCGGACTCCCATAATG 54.2 64.4

Os10g04010 STE_MEKK_ste11_MAP3K.23 Os10g04010-F AAGGGAATGCGCCTGAAGAATTCG 50 62.7

Os10g04010-R AACAATGCTACCCACAGCTTCGCC 54.2 64.4

Os10g06780 protein kinase domain containing protein Os10g06780-F TTTGGCGACCTCAAGGTTTGCGAC 54.2 64.4

Os10g06780-R AAACACTCCTCGTATGGCTTCCCG 54.2 64.4

Os10g06800 cyclin-dependent kinase G-2 Os10g06800-F TCAAGGTTTGCGACTTCGGCTCAG 54.2 64.4

Os10g06800-R CCAACCAAACACTCCTCGTATGGC 54.2 64.4

Os10g06930 mitogen-activated protein kinase 15 Os10g06930-F TGCGTCCTCAAGTACTGCGACTTC 54.2 64.4

Os10g06930-R AATTCCTCATACGGCGGCATGACC 54.2 64.4

Os10g10500 vacuolar ATP synthase 98 kDa subunit Os10g10500-F GCCTTGGATGCTGATTCCGAAACC 54.2 64.4

Os10g10500-R TGGTGCCTCTGCTCATGTTGCTTC 54.2 64.4

Os10g28240 calcium-transporting ATPase, plasma membrane-type Os10g28240-F TGTTGGTCGCTTGATTCGGCAG 54.5 62.1

Os10g28240-R TGAAGTAGTCAGCGCACTCCATCC 54.2 64.4

Os10g30070 sodium/calcium exchanger protein Os10g30070-F AGACGAAGTTCTACGGCGACCAAG 54.2 64.4

Os10g30070-R TAGTAGCTGAGTAGCGGTGCAGTG 54.2 64.4

Os10g34000 aquaporin protein Os10g34000-F GCTTGGCACGATCAGTGGATCTTC 54.2 64.4

Os10g34000-R TGCTCGTGGTATAGCGTCGCAATG 54.2 64.4

Os10g35050 aquaporin proteinaquaporin protein Os10g35050-F TGCTTCACACCCCCAACTGTACTG 54.2 64.4

Os10g35050-R TCTCACTACTCACCAACTCTCGCC 54.2 64.4

Os10g36710 CAMK_CAMK_like.40 Os10g36710-F ACTTTGAGCAGGAAGGCAACCGTC 54.2 64.4

Os10g36710-R AGATTCATCTCCTGTGCCAGCTCC 54.2 64.4

Os10g36924 aquaporin protein Os10g36924-F AAATCCGCGTGAATATGGGCCG 54.5 62.1

Os10g36924-R TACACGCCCCCAAGGTCTCATTTG 54.2 64.4

Os10g38950 CGMC_MAPKCMGC_2_ERK.14 Os10g38950-F GGCGAGGAAGTTGCCATCAAGAAG 54.2 64.4

Os10g38950-R GTCCGCTTGGCATCGATATGGTTG 54.2 64.4

Os10g39420 CAMK_CAMK_like.8 Os10g39420-F GCTTCAAAAGGCTTGCATGGAACG 50 62.7

Os10g39420-R GCCACCCAAGTCCAAAGTTGTTGC 54.2 64.4

Os10g41510 calcineurin B Os10g41510-F CGCAGCAGCTTGGAAGCGTATAAC 54.2 64.4


40

Os10g41510-R AACGCTAATATCCTGTGCGACGAC 50 62.7

Os11g01580 sodium/calcium exchanger protein Os11g01580-F TTCTACGGCGACCAAGAACCACTC 54.2 64.4

Os11g01580-R AGTTTCCTGACATGCGCTGACTCC 54.2 64.4

Os11g02240 CAMK_KIN1/SNF1/Nim1_like.4 Os11g02240-F ACCATGCGGTTGGTGGCTCATAAG 54.2 64.4

Os11g02240-R CCGTGTTGCCATGACCTCATGAAG 54.2 64.4

Os11g02300 CPuORF36 - conserved peptide uORF-containing transcript Os11g02300-F GCAACAAGATCGACCACAAGCACC 54.2 64.4

Os11g02300-R AAGTGGCATCAAAGTGAACGGCCC 54.2 64.4

Os11g03970 CAMK_KIN1/SNF1/Nim1_like.5 Os11g03970-F TGCTCTTCTTCGCATCTCAGCAGC 54.2 64.4

Os11g03970-R GCAATGCAATTCGATCTGCGGC 54.5 62.1

Os11g04170 CAMK_CAMK_like.42 Os11g04170-F GAGCACCTTTACACGGCATTCCAG 54.2 64.4

Os11g04170-R CCTTGTTCCTTCAAGGCTTGCTCG 54.2 64.4

Os11g04460 calcium-transporting ATPase, plasma membrane-type Os11g04460-F AGGAACAGAGGTTGCCAAGGAGAG 54.2 64.4

Os11g04460-R AAACTGCACGACCCCATCTTGC 54.5 62.1

Os11g05070 sodium/calcium exchanger protein Os11g05070-F GCTGATGCATACAGCCCTTCACAC 54.2 64.4

Os11g05070-R ATCCACAGGAACATGCCAGACCAG 54.2 64.4

Os11g05630 CBL-interacting protein kinase Os11g05630-F AACGCCTTCCACCTGATCTCC 57.1 61.8

Os11g05630-R ATGCTCGAACAGCGACGACAG 57.1 61.8

Os11g06890 vacuolar ATP synthase Os11g06890-F ACACAACAAAACCAAACCCGACAG 45.8 61.0

Os11g06890-R TCGAAGATGGGAAAGGGAGGAGG 56.5 64.2

Os11g07040 CAMK_CAMK_like.43 Os11g07040-F TGAATCTCTGCACGCCTCATCAGC 54.2 64.4

Os11g07040-R AATTCACTCAACGGCTTTGCCCTC 50 62.7

Os11g10100 STE_MEKK_ste11_MAP3K.3 Os11g10100-F GGCCAAGCATATATCAGCGCACAC 54.2 64.4

Os11g10100-R AGCCAAGGCTCCAAATATCGACCG 54.2 64.4

Os11g17080 OsMPK15 - Putative MAPK based on amino acid sequence homology

Os11g17080-F CAAATGCTGAAGAGGCTCTTGCCG 54.2 64.4

Os11g17080-R TGTCACAGGCTGTGCTGAAGGTTC 54.2 64.4

Os11g29490 plasma membrane ATPase Os11g29490-F ACTTTTCGCACATTCACGCGATGG 50 62.7

Os11g29490-R CAATGACGGTTGCCACCAGTTGAG 54.2 64.4

Os11g29750 plasma membrane ATPase Os11g29750-F AATGTTATAGAGCACCGCTCCCGC 54.2 64.4

Os11g29750-R ACGAAGCATCAATGCAGGGTCACC 54.2 64.4

Os11g42790 transporter, monovalent cation:proton antiporter-2 family Os11g42790-F AGTTGCTGCTGGACTTCTCAGTGC 54.2 64.4

Os11g42790-R TTCACGATCAGTGGAATGCCTGCC 54.2 64.4

Os11g43860 sodium/calcium exchanger protein Os11g43860-F ACGCAATCCGAAATCTGGGTCAGC 54.2 64.4

Os11g43860-R TCAAATGCAGCAGAACCCACAAGG 50 62.7

Os12g02200 CAMK_KIN1/SNF1/Nim1_like.6 Os12g02200-F GCGATTCATTGGAGTACCGACAGC 54.2 64.4

Os12g02200-R TTCATCTCCATGCCAGGCCAAGAC 54.2 64.4


41

Os12g02250 STE_PAK_Ste20_Slob_Wnk.3 Os12g02250-F TCCAAAATTCTCCGGCCTCACCTC 54.2 64.4

Os12g02250-R ACTTAGGCACCTCCTAACGCAAGC 54.2 64.4

Os12g03810 CAMK_KIN1/SNF1/Nim1_like.37 Os12g03810-F ACATGCTGTTGCTGCCACCACTAC 54.2 64.4

Os12g03810-R ATGGCTGCTGAGATCAGGAAGAGG 54.2 64.4

Os12g03970 CAMK_CAMK_like.44 Os12g03970-F GAGCACCTTTACACGGCATTCCAG 54.2 64.4

Os12g03970-R CCTTGTTCCTTCAAGGCTTGCTCG 54.2 64.4

Os12g06510 calcineurin B Os12g06510-F TGCACGGGCTCTTTCTGTGTTTC 52.2 62.4

Os12g06510-R ACCACCATTTGCTTCACCTCTTGC 50 62.7

Os12g06570 cyclic nucleotide-gated ion channel Os12g06570-F ATCGTCAAGTCCAGTGGAGTCGTC 54.2 64.4

Os12g06570-R CCAAGAACATGGCTTGCCAGTGTG 54.2 64.4

Os12g07140 expressed protein Os12g07140-F AAAACCGTCCAAACCCAGCCAC 54.5 62.1

Os12g07140-R TTGGGGATTGAGGAGGAGGAAGAC 54.2 64.4

Os12g07230 CAMK_CAMK_like.45 Os12g07230-F CCTGCGAACAGTTTGGCCTTTCTG 54.2 64.4

Os12g07230-R CCACCAGCATTGCCCTTTCTCATC 54.2 64.4

Os12g12860 CAMK_CAMK_like.46 Os12g12860-F CCTTGTTCGCGTATTGGTCTCCAC 54.2 64.4

Os12g12860-R AACATTCAGGGTCACTGAACCCCG 54.2 64.4

Os12g28260 cyclic nucleotide-gated ion channel Os12g28260-F CCTACTACCTCCTCCTCTACATGC 54.2 64.4

Os12g28260-R CTGATGGAACTTGAGCACCCTG 54.5 62.1

Os12g30150 CAMK_CAMK_like.47 Os12g30150-F AGGCAGTTCTCGGCCATGAACAAG 54.2 64.4

Os12g30150-R GTTCTCCGCGATCACTCTCAATGC 54.2 64.4

Os12g37570 protein kinase family protein Os12g37570-F AGATTGAGCAAGACGAGGGAGCTG 54.2 64.4

Os12g37570-R CCTTGGTCAGAATTCCGAAGCTGC 54.2 64.4

Os12g39660 calcium-transporting ATPase, plasma membrane-type Os12g39660-F AGGAGCTGAACTCTCCATGCAGTC 54.2 64.4

Os12g39660-R ATTGCCTATTCCCACTGCTTGCCC 54.2 64.4

Os12g40510 calcineurin B Os12g40510-F CAAGCGAGTACAAGTAGACGCACG 54.2 64.4

Os12g40510-R ATCGAGGATGAACTGGCTGCGATG 54.2 64.4

Os12g41090 CAMK_KIN1/SNF1/Nim1_like.38 Os12g41090-F TGAAGGTGTTTGTGGTTGGTGTCC 50 62.7

Os12g41090-R CCAAGCTCATACTTGCCGAGAAGC 54.2 64.4

Os12g42910 sodium/calcium exchanger protein Os12g42910-F TTCTCACGCCATTGCCTTCACCAC 54.2 64.4

Os12g42910-R CACTTGACACCAACCAACCCAACC 54.2 64.4

Os12g43940 ankyrin repeat domain-containing protein Os12g43940-F CATGTTGGAAGCTGGTGCAAACCC 54.2 64.4

Os12g43940-R TCTATTGGCATTCTCCCGAGCCTG 54.2 64.4

Os12g44150 plasma membrane ATPase Os12g44150-F TCCATACGCTCAAGGGGCATGTAG 54.2 64.4

Os12g44150-R AGGCTGGATCACACGGTGTAAGAC 54.2 64.4

Os12g44360 sodium/hydrogen exchanger 7 Os12g44360-F ATGAGCCTGTGCATCCTCATGCTG 54.2 64.4

SOS1 Os12g44360-R GCTTGGACACCATTTAGAAGCCGC 54.2 64.4


42

Supplemental Table 8. Sequences of OligonucleotidesUsed for Expression Profiling of Transcription Factor Genes with a Known Role in

Salt Tolerance.

Locus Name Oligonucleotide sequence 5'→3' GC content (%)

Tm (°C)

Os01g07120 DREB2A Os01g071201_F AGGGCAATGTATGGTCCCACAG 62.1 54.4

Os01g071201_R TGCTGATGTGCAGCCAGAGTTG 62.1 54.5

Os01g50910 Lea Os01g509101_F TACAAGCCTGGAACTGGGAGTG 62.1 54.5

Os01g509101_R TGGCATCAGTGCCAAAGACGAC 62.1 54.5

Os01g54600 WRKY13 Os01g546001_F CAAATTGCGTCGCGTGTTCGTC 62.1 54.5

Os01g546001_R TTGGAATCACGTCGCCAACCAC 62.1 54.5

Os01g58420 AP37 Os01g584201_F ACGTCCAGCAACGCATCCTTAC 62.1 54.5

Os01g584201_R TCATACGGTATCCAGCCTCAGC 62.1 54.5

Os01g62190 ZFP179 Os01g621901_F AGAGAAGAAGCGGAGAGCAAGG 62.1 54.5

Os01g621901_R TGCACGTCTTGCACTCGAACAC 62.1 54.5

Os01g64000 OsABI5 Os01g640001_F CTCGTCTCAAAGAGGCAGAGAAG 62.4 54.4

Os01g640001_R TCTTCTCCTTGGACTGCTCCATC 62.4 54.4

Os01g66120 SNAC2 Os01g661201_F AGAAGAACAGCCTCAGGTTGGATG 62.7 50.0

Os01g661201_R AGCCCGCCCTTCTTGTTGTAAATC 62.7 50.0

Os02g33770 OsGTc-1 Os02g337701_F GCATCGCATTTCAGGCTGCTTC 62.1 54.5

Os02g337701_R TGGCAGCTGTTGCAATCACGAG 62.1 54.5

Os02g52780 bZIP23 Os02g527801_F GGAGCTGAACGATGAACTCCAG 62.7 50.0

Os02g527801_R TCGGCTCATTCTCTCTAGAACCTC 62.7 50.0

Os03g17700 OsMAPK5 Os03g177001_F CCGCTGCAGAGAATCACAGTTG 62.1 54.5

Os03g177001_R ATCGGCGATGTCGTGCAATCTC 62.1 54.5

Os03g60080 SNAC1 Os03g600801_F GCCAAGAAGGGATCTCTCAGGTTG 64.4 54.2

Os03g600801_R TCTTCTTGTTGTACAGCCGACAC 60.6 47.8

Os03g60560 ZFP182 Os03g605601_F TGGAGTTGTTCGCCTAGTTACCG 62.4 54.4

Os03g605601_R GTGACACACACTGCGTCAGTTC 62.1 54.5

Os05g46480 LEA3 Os05g464801_F TTTCTGACGGGTGTGGGTGATG 62.1 54.5

Os05g464801_R AACACAGACGAGAAACTCTGACG 60.6 47.8

Os11g08210 OsNAC5 Os11g082101_F TTAACGGAACGTGACGCCTCTG 62.1 54.5

Os11g082101_R ACGATCGACCTGACAGTGGTTAG 62.4 52.2

Os11g26760 dehydrin Os11g267601_F GCGTGAAATGCGAGTGATGGTG 62.1 54.5

Os11g267601_R ACGCGAGAACACAACAGACAATG 60.6 47.8

Os12g39400 ZFP252 Os12g394001_F CCCTTGCAAGCTCAAGAAACCC 62.1 54.5


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Os12g394001_R GCCTCCTCCTCACTACTACTTCTC 64.4 54.2

Os05g34730 SERF1 second primer pair

Os05g347301_F GAGTGAGGAGCTCATTGTTTACGA 61.0 45.8

Os05g347301_R ACATCAAAATTTCCATGTCATCTA 54.2 29.2


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Supplemental Table 9. Probes Used for EMSA. BS, binding site. * Cy5-labelled.

Probes Sequence 5'→3' Target Note MAPK5_M1_F* TCCGCTGTACCCGCCGACGAAACGCACGCGCGAAAA MAPK5 BS1, labelled

MAPK5_M1_F TCCGCTGTACCCGCCGACGAAACGCACGCGCGAAAA MAPK5 BS1, unlabelled

MAPK5_M1_R TTTTCGCGCGTGCGTTTCGTCGGCGGGTACAGCGGA MAPK5 BS1, unlabelled

MAPK5_M2_F* TTCGTCCGCCTCCGTCGGTCCGTCCCCATCGC MAPK5 BS2, labelled

MAPK5_M2_F TTCGTCCGCCTCCGTCGGTCCGTCCCCATCGC MAPK5 BS2, unlabelled

MAPK5_M2_R GCGATGGGGACGGACCGACGGAGGCGGACGAA MAPK5 BS2, unlabelled

DREB2A_D1_F* CACACCACCACCGACACCGACACGCTCGCCAT DREB2A BS1, labelled

DREB2A_D1_F CACACCACCACCGACACCGACACGCTCGCCAT DREB2A BS1, unlabelled

DREB2A_D1_R ATGGCGAGCGTGTCGGTGTCGGTGGTGGTGTG DREB2A BS1, unlabelled

SERF1_F* GCGGTCAGCGCCGACGACGAGCAAG SERF1 labelled

SERF1_F GCGGTCAGCGCCGACGACGAGCAAG SERF1 unlabelled

SERF1_R CTTGCTCGTCGTCGGCGCTGACCGC SERF1 unlabelled

ZFP179_F* CGCATGCTCCTCTCGCCGACGTGGTCTACAC ZFP179 labelled

ZFP179_F CGCATGCTCCTCTCGCCGACGTGGTCTACAC ZFP179 unlabelled

ZFP179_R GTGTAGACCACGTCGGCGAGAGGAGCATGCG ZFP179 unlabelled

MAP3K6_F* TAGGCCAAGCCCGAGCCGACGGCGCCGCAG MAP3K6 labelled

MAP3K6_F TAGGCCAAGCCCGAGCCGACGGCGCCGCAG MAP3K6 unlabelled

MAP3K6_R CTGCGGCGCCGTCGGCTCGGGCTTGGCCTA MAP3K6 unlabelled


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Supplemental Table 10. Probes Used for ChIP-qPCR. NC, negative control. BS, binding site.

Oligo Sequence 5'→3' Target Note SERF1_ChIP_F ACACACTCGCCGTCTTGTCT SERF1 BS1

SERF1_ChIP_R CCCATGTACGTACCGTCTTCC SERF1 BS1

ZFP179_ChIP_F AAGTGGTCGTTGTCCCACAC ZFP179 BS1

ZFP179_ChIP_R GTAGACCACGTCGGCGAGAG ZFP179 BS1

MAP3K6_ChIP_F CCTGCGAGTAACTCCACAAATG MAP3K6 BS1

MAP3K6_ChIP_R CATCCTTCACCCTCAACGTG MAP3K6 BS1

DREB2A_ChIP_F TGTTAGAGAGGAGGGCACACAC DREB2A BS1

DREB2A_ChIP_R CCTATTCCTCCTCCGTTCAGAT DREB2A BS1

MAPK5-M1_ChIP_F CTGTACCCGCCGACGAAAC MAPK5 BS1

MAPK5-M1_ChIP_R GTAGACTCTGCACCACCCTGTA MAPK5 BS1

MAPK5-M2_ChIP_F CATAACCCACCAGCTGAACAC MAPK5 BS2

MAPK5-M2_ChIP_R GGTGTGGTGCGTGGATTTC MAPK5 BS2

NC_ChIP_F GAAATTGGAACAATGTGATGGAA NC ZFP179 promoter (-2.1 kb)

NC_ChIP_R TCATATAAGCACTATCGCCGTGT NC ZFP179 promoter (-2.1 kb)


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Zea mays Brachypodium distachyon Oryza sativa - Plant Cell · Supplemental Figure 1. SERF1 protein...

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