TNF
1
TNF ng/ml 0 2 4 6 ** * IL-6 0 50 100 150 ng/ml * IL-8 0 100 200 300 ng/ml * IL-10 0 0.5 1 1.5 2 ng/ml IFN-γ 0 50 100 150 ng/ml * * * AL- 3×10 7 /ml +WT 3×10 7 /ml +WT 3×10 6 /ml +WT 3×10 5 /ml AL- 3×10 7 /ml +WT 3×10 7 /ml +WT 3×10 6 /ml +WT 3×10 5 /ml AL- 3×10 7 /ml +WT 3×10 7 /ml +WT 3×10 6 /ml +WT 3×10 5 /ml AL- 3×10 7 /ml +WT 3×10 7 /ml +WT 3×10 6 /ml +WT 3×10 5 /ml AL- 3×10 7 /ml +WT 3×10 7 /ml +WT 3×10 6 /ml +WT 3×10 5 /ml FIG. S3. FIG. S3. Presence of autolysed WT pneumococci, dose- dependently inhibit production of TNF and IFN-γ in response to AL- intact pneumococci. PBMC from six blood donors were stimulated intact D39 AL- at 3 × 10 7 bacteria/ml mixed with 3 × 10 5 , 3 × 10 6 and 3 × 10 7 bacteria/ml (before autolysis) of autolysed D39 WT. Cytokines were measured in the supernatant after 24 h (TNF, IL-6, IL-8 and IL-10) or 5 d (IFN-γ). Medians and quartiles are shown by boxes and the bars represent the 10th and 90th percentiles, respectively. The response to AL- bacteria with and without addition of WT bacteria within the same donor was compared using Wilcoxon matched pairs test. * p < 0.05; ** p < 0.01.
description
TNF. IFN- γ. *. *. *. **. *. 150. 6. 100. ng/ml. ng/ml. 4. 50. 2. 0. 0. +WT 3 ×10 7 /ml. AL- 3 ×10 7 /ml. +WT 3 ×10 6 /ml. +WT 3 ×10 5 /ml. +WT 3 ×10 7 /ml. AL- 3 ×10 7 /ml. +WT 3 ×10 6 /ml. +WT 3 ×10 5 /ml. IL-6. IL-8. IL-10. *. *. 2. 300. 150. 1.5. 200. ng/ml. - PowerPoint PPT Presentation
Transcript of TNF
TNF
ng/m
l
0
2
4
6
***
IL-6
0
50
100
150
ng/m
l
*IL-8
0
100
200
300
ng/m
l
*IL-10
0
0.5
1
1.5
2
ng/m
l
IFN-γ
0
50
100
150
ng/m
l
**
*
AL- 3×10
7 /ml
+WT 3×
107 /m
l
+WT 3×
106 /m
l
+WT 3×
105 /m
l
AL- 3×10
7 /ml
+WT 3×
107 /m
l
+WT 3×
106 /m
l
+WT 3×
105 /m
l
AL- 3×10
7 /ml
+WT 3×
107 /m
l
+WT 3×
106 /m
l
+WT 3×
105 /m
l
AL- 3×10
7 /ml
+WT 3×
107 /m
l
+WT 3×
106 /m
l
+WT 3×
105 /m
l
AL- 3×10
7 /ml
+WT 3×
107 /m
l
+WT 3×
106 /m
l
+WT 3×
105 /m
l
FIG. S3.
FIG. S3. Presence of autolysed WT pneumococci, dose-dependently inhibit production of TNF and IFN-γ in response to AL- intact pneumococci. PBMC from six blood donors were stimulated intact D39 AL- at 3 × 107 bacteria/ml mixed with 3 × 105, 3 × 106 and 3 × 107 bacteria/ml (before autolysis) of autolysed D39 WT. Cytokines were measured in the supernatant after 24 h (TNF, IL-6, IL-8 and IL-10) or 5 d (IFN-γ). Medians and quartiles are shown by boxes and the bars represent the 10th and 90th percentiles, respectively. The response to AL- bacteria with and without addition of WT bacteria within the same donor was compared using Wilcoxon matched pairs test. * p < 0.05; ** p < 0.01.
