γλώσσες
Σελίδες
Νομικός
TNF
ng/m
l
0
2
4
6
***
IL-6
0
50
100
150
ng/m
l
*IL-8
0
100
200
300
ng/m
l
*IL-10
0
0.5
1
1.5
2
ng/m
l
IFN-γ
0
50
100
150
ng/m
l
**
*
AL- 3×10
7 /ml
+WT 3×
107 /m
l
+WT 3×
106 /m
l
+WT 3×
105 /m
l
AL- 3×10
7 /ml
+WT 3×
107 /m
l
+WT 3×
106 /m
l
+WT 3×
105 /m
l
AL- 3×10
7 /ml
+WT 3×
107 /m
l
+WT 3×
106 /m
l
+WT 3×
105 /m
l
AL- 3×10
7 /ml
+WT 3×
107 /m
l
+WT 3×
106 /m
l
+WT 3×
105 /m
l
AL- 3×10
7 /ml
+WT 3×
107 /m
l
+WT 3×
106 /m
l
+WT 3×
105 /m
l
FIG. S3.
FIG. S3. Presence of autolysed WT pneumococci, dose-dependently inhibit production of TNF and IFN-γ in response to AL- intact pneumococci. PBMC from six blood donors were stimulated intact D39 AL- at 3 × 107 bacteria/ml mixed with 3 × 105, 3 × 106 and 3 × 107 bacteria/ml (before autolysis) of autolysed D39 WT. Cytokines were measured in the supernatant after 24 h (TNF, IL-6, IL-8 and IL-10) or 5 d (IFN-γ). Medians and quartiles are shown by boxes and the bars represent the 10th and 90th percentiles, respectively. The response to AL- bacteria with and without addition of WT bacteria within the same donor was compared using Wilcoxon matched pairs test. * p < 0.05; ** p < 0.01.
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