PCR & Molecular Biology · a &doleudwlrq ulqjv dw b o b o dqg b o a ,ghdoo\ vxlwhg wr slshwwlqj...
40
Liquid Handling PCR & Molecular Biology
Transcript of PCR & Molecular Biology · a &doleudwlrq ulqjv dw b o b o dqg b o a ,ghdoo\ vxlwhg wr slshwwlqj...
Liquid Handling
PCR & Molecular Biology
Your Sarstedt Team
2
®
®
Compatibility table
®
®
®
®
Compatibility table
®
PCR Performance Tested Quality • The Sarstedt quality and purity standard
PCR Performance Tested
✓ DNA-free ✓ DNase/RNase-free ✓ PCR inhibitor-free
Human DNA < 0.5 pg/μl · Bacterial DNA < 0.02 pg/μl · DNase < 1x10-5 U/μlRNase < 1x10-9 Kunitz units/μl
Biosphere® plus quality • Unrivalled purity
®
®
®
Biosphere® plus
✓ Sterile ✓ DNA-free ✓ DNase/RNase-free✓ PCR inhibitor-free ✓ ATP-free ✓ Non-pyrogenic /endotoxin-free
Human DNA < 5.0 fg/μl · Bacterial DNA < 0.2 fg/μl · DNase < 5x10-7 U/μlRNase < 5x10-11 Kunitz units/μl · ATP < 1x10-12 mmol/μl · Pyrogens < 0.002 EU/mlSterility validated in accordance with ISO 11135
®
Conformity testing in accordance with the international test standard ISO 8655-2
®
®
®
Piston
Pipette shaft
Aerosol
Aerosol-free
Filter
Sample
®
®
®
Tailored tip solutions for every application
•
•
•
•
•
•
•
•
•
®
•
Tip:to easily identify which
tip is suitable for your pipette, use the compatibility table on
pages 18-21or at www.sarstedt.com
A0
1
2
3
4
5
loss
(rel
. uni
ts)
AB B C DSARSTEDT
2
®
Product(Illustrations: actual size)
Tray colour code
Order no. VolumePurity Grade
Packaging
Type B
ProductTray colour
codeOrder no. Volume Packaging
Type N
Type J
Type A
Type L
Type B
Type E
Type E
Type E
Type N
Type J
Type A
Type A
Type C
Type L
Order no. DesignPackaging Pcs./case
Simple and practical
Economical and environmentally friendly
Tip StackPack packaging
reducing packaging and storage space required in the Tip SystemBox
Ecen
TippEmpty 96-tip reload trays and bulk-bagged tips
Suitable for manually racking bulk tips for autoclaving and convenient handling.
Order no. Tray colour code Tip type/volumePackaging Pcs./case
Order no. Volume Purity Grade Packaging
Filter tips(Illustrations: actual size)
Tray colour code
Order no.Max. sample
volume/colourPurity Grade
Packaging
Type E
Type E
Type N
Type J
Type A
Type A
Type C
Type L
illustration 1:2
Type M
Type B
illustration 1:2
Type O
atttiiiioion 1:2
Extra long
Tips without filters(Illustrations: actual size)
Tray colour code
Order no.Max. sample
volume/colourPurity Grade
Packaging
Type E
Type N
Type J
Type A
Gelloaderillustration 1:2
Type C
Type L
illustration 1:2
Type M
illustration 1:2
illustration 1:2
illustration 1:2
Type B
illustration 1:2
Type O
ratattttiiion 1:2
Extra long
● = compatible 10 = limited pipetting volume ✗ = not recommended
= not checked MC = multi-channel pipette
®
® ® ®
0.1-
2.5 μl
0.5-
10 μ
l
2-20
μl
2-20
μl
50-2
00 μ
l
100-
1,00
0 μl
0.1-
2.5 μl
100-
1,00
0 μl
1-10
ml
0.1-
2.5 μl
● ●2.5
●2.5 ● ●
2.5 ● ●2.5
●2.5
● ●10 ✗ ● ✗ ● ●
10
● ● ● ● ● ● ● ●
● ● ● ● ● ● ● ●
● ●20
●20 ✗ ●
20●20 ✗ ● ●
20 ● ✗
● ● ●100 ● ● ●
100 ✗ ● ● ●100 ✗
● ● ● ● ● ● ● ● ●
✗ ✗ ● ✗ ✗ ● ✗ ✗ ✗ ● ✗
✗ ✗ ● ✗ ✗ ● ● ● ●
● ●
● ●
® ® ®
● ● ● ● ● ● ● ●10
● ● ● ✗ ● ● ● ●
● ● ● ● ● ● ● ●
● ● ● ● ● ● ✗ ● ● ● ✗
● ● ● ● ● ● ● ● ●
● ● ● ● ✗ ● ● ✗
✗ ✗ ● ✗ ✗ ● ● ✗ ✗ ● ●
● ●
● ●
5 m
l
✗
5 m
l
●
10 m
l
●
® ® ®
®
Xplor
er®
® ®®
Bio
Cont
rol
®
100-
1,00
0 μl
1-10
ml
0.5-
10 μ
l
50-1
000 μl
100-
5000
μl
100-
1,00
0 μl
200-
1,00
0 μl
1-10
μl
30-3
00 μ
l
100-
1,00
0 μl
30-3
00 μ
l
100-
1,00
0 μl
P10/
U10
0.1-
P100
/U10
0
P200
/U20
020
-200
μl
P1.0
00/U
3.00
020
0-1,
000 μl
P5.0
00/U
5.00
010
00-5
000 μl
P10/
U10
1-10
ml
●2.5
●2.5 ● ●
2.5●2.5 ● ●
2.5●2.5 ● ●
2.5 ✗
● ● ● ● ● ● ● ● ● ✗
● ● ● ● ● ● ● ● ● ● ✗
● ● ● ● ● ● ● ● ● ● ✗
●20
●20 ● ●
20 ✗ ● ●20
● ● ● ●100
●100 ✗ ✗ ● ●
● ● ● ● ● ● ●
●200
●200 ✗ ✗ ● ●
200●
200●
200 ✗ ✗ ● ✗
● ● ✗ ✗ ● ● ● ● ✗ ✗ ● ●
● ● ✗ ● ● ● ● ●
● ● ● ● ● ● ● ●
®
Xplor
er®
® ®®
Bio
Cont
rol
®
● ● ● ● ● ● ● ● ● ● ● ✗
● ● ● ● ● ● ● ● ● ● ● ✗
● ● ● ● ● ● ● ● ● ● ✗
● ● ● ● ● ●200 ✗ ● ● ● ●
200
● ● ● ● ● ● ●
●250
●250 ✗ ✗ ● ●
250 ✗ ✗ ● ●250
✗ ✗ ● ● ✗ ✗ ● ● ● ● ● ● ✗ ✗ ● ●
● ● ✗ ● ● ● ● ✗ ●
● ● ● ● ● ● ● ●
✗ ✗ ●
● ● ✗
● ●
® ® ®
● ● ● ● ● ●
●
● ● ● ● ● ✗
✗ ● ✗ ● ● ✗ ✗ ✗ ✗
● ● ● ● ●
✗ ● ● ● ● ● ●250 ✗
✗ ● ● ✗ ✗ ● ● ● ●
● ● ● ✗
● ● ✗
5 m
l
✗ ✗ ✗
5 m
l
● ● ●
10 m
l
®
® ® ®
0.5-
10 μ
l
20-2
00 μ
l
100-
1,00
0 μl
100-
5,00
0 μl
0.1-
2.5 μl
0.5-
10 μ
l
100-
1,00
0 μl
100-
5,00
0 μl
0.2-
10 μ
l
50-1
,000
μl
0.1-
1 μl
●2.5
●2.5 ● ●
2.5●2.5 ✗
● ● ● ● ✗
●
● ● ● ● ● ✗
✗ ✗ ✗ ●20
●20 ✗ ✗ ✗ ✗
✗ ●100 ● ● ●
100 ✗ ✗ ✗
● ● ● ● ●
✗ ● ✗ ✗ ● ●200
●200 ✗
✗ ● ● ✗ ✗ ● ● ● ●
● ● ● ✗
● ● ✗
● = compatible 10 = limited pipetting volume ✗ = not recommended
= not checked MC = multi-channel pipette
® ®® ® ®
®
A. 8
35/
935e
®
● ● ✗ ● ● ● ● ● ● ● ● ● ● ● ●
● ● ● ● ● ●
● ● ✗ ● ● ● ● ● ● ● ● ✗ ● ●
● ● ● ✗ ✗ ● ● ● ✗ ● ● ● ● ● ● ● ● ● ● ● ● ● ●
● ● ● ●
● ● ● ✗ ✗ ● ● ● ✗ ● ✗ ✗ ✗ ● ✗ ✗ ✗ ✗ ✗ ✗ ● ✗ ●
✗ ✗ ✗ ● ● ✗ ✗ ● ✗ ● ✗ ✗ ✗ ● ✗ ✗ ✗ ✗ ● ● ● ✗ ●
● ● ● ● ● ●
● ● ● ● ● ●
✗
●
● ✗
® ®
® ®® ® ®
®
A. 8
35/
935e
®
0.5-
10 μ
l
2-20
μl
2-20
μl
20-2
00 μ
l
100-
1,00
0 μl
0.1-
1 μl
0.5-
10 μ
l
20-2
00 μ
l
100-
1,00
0 μl
1-10
ml
0.5-
10 μ
l
100-
1,00
0 μl
1-10
μl
1-10
μl
2-20
μl
20-2
00 μ
l
100-
1,00
0 μl
1-10
μl
20-2
00 μ
l
0.1-
2 μl
0.5-
10 μ
l
1-10
μl
2-20
μl
100-
1,00
0 μl
0.5-
5 m
l
●2.5
●2.5 ✗ ●
2.5●2.5
●2.5
●2.5
●2.5
●2.5 ● ●
2.5●2.5 ● ✗ ●
2.5
● ●10 ✗ ● ● ● ● ● ● ● ● ● ● ● ●
● ● ✗ ● ● ● ●
● ● ✗ ● ● ● ● ● ● ✗ ✗ ✗ ● ●
● ● ●20 ✗ ✗ ● ●
20●20 ✗ ●
20●10 ● ●
20 ✗ ● ✗ ● ● ●20
●20 ✗ ● ✗
✗ ✗ ✗ ✗ ✗ ✗ ✗ ●100 ✗ ●
100 ✗ ✗ ✗ ●100 ✗ ● ✗ ✗ ● ● ●
100 ● ●100
● ● ● ●
✗ ✗ ✗ ✗ ✗ ✗ ✗ ● ✗ ●100 ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ● ✗ ●
✗ ✗ ✗ ● ● ✗ ✗ ● ✗ ● ✗ ✗ ✗ ● ✗ ✗ ✗ ✗ ✗ ✗ ● ✗ ●
✗ ● ● ● ● ●
✗ ● ● ● ● ●
®
®
®
Can be opened without touching the inside of the lid
®
22
®
®
Product Order no. Vol. Colour Quality GradeUnits/bag Units/case
®
Product Order no.Multiply® μStrip,
suitable forColour Quality Grade
Units/bag Units/case
®
Product Order no. Vol. Profile Colour Lid stripQuality Grade
Units/bag Units/case
®
®
Product Order no. Product description Design Packaging unit
®
®
Order no. Capacity Format Suitable for Packaging
®
t
Flu
ore
sc
en
ce
Transparent wells
White wells
CyclesC
t
Ct
Number of plasmid copies
Ct values for the detection of plasmid copies in white and transparent
plates at reduced reaction volumes
Ct v
alu
es
• Fluorescence level 10x higher
• Greater sensitivity
• Improved reproducibility
Multiply®
®
Order no. Colour Skirt Deck Profile Well Max. volumeUnits/bag Units/case
Order no. Colour Skirt Deck Profile Well Max. volumeUnits/bag Units/caseUnits/bag
Tip: to easily identify which
plate is suitable for your thermocycler use our
compatibility table on page 30/31 or at
www.sarstedt.com.
Order no. Colour Skirt Deck Profile Well Max. volumeUnits/bag Units/case
Order no. Colour Skirt Deck Profile Well Max. volumeUnits/bag Units/case
®
®
Order no. Colour Skirt Deck Profile Well Max. volume Blister packs/box
Order no. Colour Skirt Deck Profile Well Max. volumeUnits/bag Units/case
®
Order no. Colour Skirt Deck Well Max. volumeUnits/bag Units/case
Product Order no. Description Design ColourUnits/bag Units/case
Order no. Colour Skirt Deck Profile Well Max. volumeUnits/bag Units/case
Order no. Colour Skirt Deck Profile Well Max. volumeUnits/bag Units/case
Order no. Colour Skirt Deck Well Max. volumeUnits/bag Units/case
®
Number of wells 96 pre-inserted 96 96 96 96 384 full skirt
Half/full skirt Without Without Half Full Half Full
High High High Low Low -
Order no. PCR plates
Amersham Biosciences® / GE Healthcare®
●
●
Analytik Jena®/Biometra®
2 ●
● ● ●2 ● ● ●
● ●
● ● ●
● ● ●
●
● ●
●
Applied Biosystems®/Life Technologies®
® ● ●® ● ●® ● ● ●® ● ● ●® ●
● ●
● ● ●
● ● ●® ● ●® ● ●® ●® ●® ● ●® ●® ● ●
●
●
Veriti® ● ●
Veriti® ● ●
●
● ● ●
● ● ●
● ●
● ● ●
PeqLab®
● ● ●
●
®
● ● ●
● ●
●
®
Number of wells 96 pre-inserted 96 96 96 96 384 full skirt 96-well 384-well
Half/full skirt Without Without Half Full Half Full Full Full
High High High Low Low - ‘Lightcycler’ ‘Lightcycler’
Order no. PCR plates
BioRad®/MJ Research®
●
●
● ● ●
● ● ● ●
● ● ● ●
● ● ●
● ● ●
● ● ●
● ● ●
● ●
●
●
Corbett Research®/Qiagen®
●
●
Eppendorf®
® ● ●® ● ● ●® ● ● ●® ● ● ● ●® ● ● ●
Ericom®
● ●
● ●
● ●
MWG®
● ●
●
● ● ●
Roche®
® ●® ● ●
Stratagene®/Agilent®
●
● ●
● ● ● ●
● ● ●
● ●
●
Techne®
● ●
● ● ●
● ● ●
● ●
● ● ● ●
●
● ● ●
● ●
● ● ●
● ● ● ●
95.1999
95.1994
95.1993
95.1995
Order no.Product
descriptionApplication Special properties Clear Pierceable
Functional temperature range
Packaging
+
+
+
Film/foil properties Order no.
✓ ✓ ✓
BS
A lo
ss (%
)
Standard tube Tubes with low binding properties
SupplierSarstedt
Experiment method:
Sarstedt
Supplier AControl
Supplier B
Supplier C
Supplier D
Flu
ore
scen
ce
Cycle403938373635343332313029282726252423222120191817161514131211109876543210
100
1000
10000
Sarstedt
B C DAControl
Volume Order no. Description Packaging
®
Volume Order no. Purity Grade Packaging
®
®
Order no. DesignUnits/bag Units/case
®
®
®
®
®
Order no. Design Volume/materialUnits/bag Units/case
® ®
Order no. Height in mm LCH Clear sides Units/Styrofoam rack/case
2
2
2
2
Wavelength (nm)
Transmission (T) in %
UV Acrylic Polystyrene
Screw Cap Micro TubesTransport, storage and sample preparation
Inner sub-heading 14ptCell and tissue cultureCell Cultivation, Cryogenic Storage, Filtration, Liquid Handling
• Designed to accommodate a range of tube sizes in one rack
•
• Units are rotatable and freely exchangeable
• Ideal for use in the temperature range from -80°C to 121°C
• L x W x H (per unit): 108 x 108 x 257 (108 x 108 x 69) mm
Ordering Information
Art. No. Description Packaging
93.879 Revolver Workstation1/bag4/case
Revolver Workstation
SARSTEDT AG & Co.P.O. Box 12 20D-51582 NümbrechtPhone +49 2293 305-0Fax +49 2293 [email protected]
ELISA PlatesMicro Test Plates for Immunoanalytics
IVARO Tube Handler XSInnovative automation for tube processing
MicrobiologySample collection, cultivation, processing
1
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