pcDNA5 PGK pcDNA5 PGK
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pcDNA5 PGK pcDNA5 PGK + + - - + + - - + + - HIF-1α PGK APE-1 shHIF-1α shHIF-1α + dox 5ng/ml shHIF-1α shHIF-1α + dox 5ng/ml Sh-control Sh-control + dox 5ng/ml Sh-control Sh-control + dox 5ng/ml 0.0 0.5 1.0 1.5 2.0 2.5 R e la tiv e L -O ddC In co rpo ratio n 0.0 0.5 1.0 1.5 2.0 2.5 R e la tiv e L -O ddC In co rp o ratio n shPGK shPGK + dox 0.05ng/ml shPGK + dox 5ng/ml shPGK shPGK + dox 0.05ng/ml shPGK + dox 5ng/ml 0.0 0.5 1.0 1.5 2.0 L -O d d C T P pm ole/10 6 ce lls 0.0 0.5 1.0 1.5 2.0 L -O d d C T P pm ole/10 6 c ells 0.0 0.5 1.0 1.5 2.0 2.5 R e la tiv e L -O ddC In co rp o ratio n 0.0 0.5 1.0 1.5 2.0 L -O d d C T P pm ole/10 6 c ells 0.0 0.5 1.0 1.5 2.0 2.5 L -O d d C T P pm ole/10 6 c ells 0.0 0.5 1.0 1.5 2.0 2.5 R ela tiv e L -O ddC In co rp o ratio n 0 5 10 15 20 d F d C T P pm ole/10 6 ce lls 0.00 0.25 0.50 0.75 1.00 1.25 R e la tiv e d FdC In co rpo ratio n 0 5 10 15 20 d F d C T P pm ole/10 6 c ells 0.00 0.25 0.50 0.75 1.00 1.25 R e la tiv e d FdC In co rp o ratio n 0 5 10 15 20 d F d C T P pm ole/10 6 cells 0 5 10 15 20 d F d C T P p m ole/10 6 ce lls 0.00 0.25 0.50 0.75 1.00 1.25 R e la tiv e d FdC In co rp o ratio n 0.00 0.25 0.50 0.75 1.00 1.25 R e la tive d FdC In co rpo ratio n Supplemental Fig.5 Supplemental Figure 5. Effect of over-expression of PGK on the levels of L-OddCTP and dFdCTP and their DNA incorporation under normoxic and hypoxic conditions in RKO cells (First column). Effect of induction shHIF-1 using doxcycline on the HIF-1 and PGK expression and the level of L-OddC, dFdCTP and their DNA incorporation under normoxic and hypoxic conditions in RKO cells (Third column). Effect of the induction of shPGK using doxcycline on PGK expression and the levels of L-OddC, dFdCTP and their DNA incorporation under normoxic and hypoxic conditions RKO cells (Fourth column). MoL #33472
description
MoL #33472. shPGK shPGK + dox 0.05ng/ml shPGK + dox 5ng/ml shPGK shPGK + dox 0.05ng/ml shPGK + dox 5ng/ml. Sh-control Sh-control + dox 5ng/ml Sh-control Sh-control + dox 5ng/ml. shHIF-1 α shHIF-1 α + dox 5ng/ml shHIF-1 α shHIF-1 α + dox 5ng/ml. Supplemental Fig.5. pcDNA5 PGK - PowerPoint PPT Presentation
Transcript of pcDNA5 PGK pcDNA5 PGK
pcD
NA
5
PG
K
pcD
NA
5
PG
K
Hypoxia - - + + - - + + - - + + - - - + + +HIF-1α
PGK
APE-1
shH
IF-1
α
shH
IF-1
α +
do
x 5
ng
/ml
shH
IF-1
α
shH
IF-1
α +
do
x 5
ng
/ml
Sh-
con
trol
Sh-
con
trol
+ d
ox
5ng
/ml
Sh-
con
trol
Sh-
con
trol
+ d
ox
5ng
/ml
0.0
0.5
1.0
1.5
2.0
2.5
Rel
ativ
e L
-Od
dC
Inco
rpo
rati
on
0.0
0.5
1.0
1.5
2.0
2.5
Rel
ativ
e L
-Od
dC
Inco
rpo
rati
on
shP
GK
shP
GK
+ d
ox
0.0
5n
g/m
l
shP
GK
+ d
ox
5n
g/m
l
shP
GK
shP
GK
+ d
ox
0.0
5n
g/m
l
shP
GK
+ d
ox
5n
g/m
l
0.0
0.5
1.0
1.5
2.0
L-O
dd
CT
P p
mo
le/1
06 cel
ls
0.0
0.5
1.0
1.5
2.0
L-O
dd
CT
P p
mo
le/1
06 cel
ls
0.0
0.5
1.0
1.5
2.0
2.5
Rel
ativ
e L
-Od
dC
Inco
rpo
rati
on
0.0
0.5
1.0
1.5
2.0
L-O
dd
CT
P p
mo
le/1
06 cel
ls0.0
0.5
1.0
1.5
2.0
2.5
L-O
dd
CT
P p
mo
le/1
06 cel
ls
0.0
0.5
1.0
1.5
2.0
2.5
Rel
ativ
e L
-Od
dC
Inco
rpo
rati
on
0
5
10
15
20
dF
dC
TP
pm
ole
/106 c
ells
0.00
0.25
0.50
0.75
1.00
1.25
Rel
ativ
e d
Fd
CIn
corp
ora
tio
n
0
5
10
15
20
dF
dC
TP
pm
ole
/106 c
ells
0.00
0.25
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0.75
1.00
1.25
Rel
ativ
e d
Fd
CIn
corp
ora
tio
n
0
5
10
15
20
dF
dC
TP
pm
ole
/106 c
ells
0
5
10
15
20
dF
dC
TP
pm
ole
/106 c
ells
0.00
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0.50
0.75
1.00
1.25
Rel
ativ
e d
Fd
CIn
corp
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n
0.00
0.25
0.50
0.75
1.00
1.25
Rel
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e d
Fd
CIn
corp
ora
tio
n
Supplemental Fig.5
Supplemental Figure 5. Effect of over-expression of PGK on the levels of L-OddCTP and dFdCTP and their DNA incorporation under normoxic and hypoxic conditions in RKO cells (First column). Effect of induction shHIF-1 using doxcycline on the HIF-1 and PGK expression and the level of L-OddC, dFdCTP and their DNA incorporation under normoxic and hypoxic conditions in RKO cells (Third column). Effect of the induction of shPGK using doxcycline on PGK expression and the levels of L-OddC, dFdCTP and their DNA incorporation under normoxic and hypoxic conditions RKO cells (Fourth column).
MoL #33472
