Supplementary Figure S2

Transcript of Supplementary Figure S2

DMSO

1nM

10nM

100n

M

Rapamycin (24hr)

0

5

10

15

20p-AKT p-Src

DMSO

1 nM

10 n

M

100

nM

Rapamycin (3hr)

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6p-AKT p-Src

phos

pho

: tot

al ra

tio

p-AKT

p-Src

p-S6

t-AKT

t-Src

t-S6

β-act

DMSO

1nM

10nM

100n

M

Rapamycin (24hr)

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4p-AKT p-Src

MCF7 BT474 MDA-MB-231

Rapamycin induction of p-AKT occurs within 1 hour and is not due to increased levels of p-Src.

t-Src

p-AKT

t-AKT

MCF7 BT474 MDA-MB-231

p-Src

0hr 0.5hr 1hr 5hr 24hrRapamycin (10nM)

05

10152025 p-AKT p-Src

phos

pho:

tota

l rati

o

0hr

0.5h

r

1hr

5hr

24hr

Rapamycin (10nM)

02468

10 p-AKT p-Src

0hr 0.5hr 1hr 5hr 24hrRapamycin (100nM)

0

10

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40 p-AKT p-Src

A

B

Supplementary Figure S2

Documents

Transcript of Supplementary Figure S2

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