Supplementary figure 1

11
Supplementary figure 1 C . B . H3 AcK9-14 - actin 43 KD 17KD Ctrl 4h 8h 24h PB Average neurite length/cell (µm) * ** * 0 20 40 60 80 100 120 140 160 Ctrl TSA dbcAMP Ctrl dbcAMP TSA Actinomycin D * * A . 0 20 40 60 80 100 120 Ctrl PB * Average neurite length/cell (µm) Ctrl PB F actin III Tubulin Hoechst **

description

PB. Ctrl. PB. Ctrl 4h 8h 24h. 17KD. H3 AcK9-14. b -actin. 43 KD. 120. 100. 80. 60. 40. 20. 0. Supplementary figure 1. A. b III Tubulin. F actin. Hoechst. *. B. Average neurite length/cell (µm). Ctrl. PB. C. **. 160. *. 140. - PowerPoint PPT Presentation

Transcript of Supplementary figure 1

Page 1: Supplementary figure 1

Supplementary figure 1

C.

B.

H3 AcK9-14

-actin43 KD

17KD

Ctrl 4h 8h 24h

PB

Ave

rage

neu

rite

leng

th/c

ell (

µm

) *

** **

020406080

100120140160

Ctrl TSA dbcAMP Ctrl dbcAMPTSA

Actinomycin D

**

A.

0

20

40

60

80

100

120

Ctrl PB

*

Ave

rage

neu

rite

leng

th/c

ell (

µm

)Ctrl PB

F actin III Tubulin Hoechst

**

Page 2: Supplementary figure 1

Supplementary figure 2

A.

B.

TSACtrl

TSACtrl

β III TubulinHoechst

β III TubulinHoechst

Page 3: Supplementary figure 1

dbcAMP

0

20

40

60

80

100

120

Ctrl TSA PB

Ave

rage

neu

rite

leng

th/c

ell (

µm

)

Supplementary figure 3

A.

B.

Ave

rage

neu

rite

leng

th/c

ell (

µm

)

0

20

40

60

80

100

120

Actinomycin D Flavopiridol

CtrlTSA*

**

****

**

C.

actin43 KD

H3 AcK9-1417 KD

PDL

Mye

lin

Page 4: Supplementary figure 1

Supplementary figureSupplementary figure 4

RNAi CBP

RNAi P/CAF

RNAi p300

0

20

40

60

80

100

120R

T-P

CR

- ar

bitr

ary

units

A.

B.

Hoechst

GFP

CBP

Hoechst

GFP

P/CAF

GFP-Scr GFP-ScrCBP-p300 siRNA P/CAF shRNA

Page 5: Supplementary figure 1

GFP-Scr+TSA 1ng/ml

GFP-Scr+TSA 1ng/ml

CBP-p300 siRNA+TSA 1ng/ml

P/CAF shRNA+TSA 1ng/ml

C.

Hoechst

GFP

CBP

Hoechst

GFP

P/CAF

Supplementary figure 4

0

20

40

60

80

100

120

** ****

**

Pix

el in

tent

iy.-

arbi

trar

y un

its

vehicle TSA

Ctrl

CBP/p300 siRNA

P/CAF shRNA

Page 6: Supplementary figure 1

Supplementary figure 4

D.

GFP-Scr CBP-p300 siRNA P/CAF shRNA

Hoechst

GFP

H3 AcK9-14GFP-Scr+TSA 1ng/ml

CBP-p300 siRNA+TSA 1ng/ml

P/CAF shRNA+TSA 1ng/ml

E.

Page 7: Supplementary figure 1

F.%

of t

rans

fect

ed n

euro

ns w

ith a

popt

otic

feat

ures

(f

ragm

ente

d or

pic

notic

nuc

lei)

0

10

20

30

40

50

GFP

-scr

CB

P/p

300

siR

NA

P/C

AF

shR

NA

Supplementary figure 4

G.

0

10

20

30

40

50

% o

f tra

nsfe

cted

neu

rons

with

cle

aved

Cap

spas

e 3

stai

ning

GFP

-scr

CB

P/p

300

siR

NA

P/C

AF

shR

NA

Page 8: Supplementary figure 1

Supplementary figure 5

CBPAcH3 K9-14 Hoechst

A.

B.

0

20

40

60

80

100

120

Ctrl TSA

CtrlTSA

**

Arb

itrar

y U

nits

0

20

40

60

80

100

120

140

High Low

Arb

itrar

y U

nits

**

TSA

AcH3K9-14

0

10

20

30

40

50

60

70

80

High Low

Ave

rage

neu

rite

leng

th/c

ell

**

TSA

AcH3K9-14

p300AcH3 K9-14 Hoechst

C. D.

Page 9: Supplementary figure 1

Supplementary figure 6

A.

p53 putative binding site

Trans. starting site

SCG-10

Chl1

L1cam

CAP-23

Lgals1

Tub1a

+1

+1

+1

+1

+1

+1

-4000 -5000 -3000 -2000 -1000

Page 10: Supplementary figure 1

CAP 23

0

1

2

3

4

Ctrl 4h 8h 24h

Lcam1

Lgals1

SCG10

Chl1

0

1

2

3

4

Ctrl 4h 8h 24h

*

0

1

2

3

4

Ctrl 4h 8h 24h

0

1

2

3

4

Ctrl 4h 8h 24h

****

0

1

2

3

4

Ctrl 4h 8h 24h

**

*

0

1

2

3

4

Ctrl 4h 8h 24h

**

*

Supplementary figure 6

B.F

old

chan

ge T

SA

/ ve

hicl

eF

old

chan

ge T

SA

/ ve

hicl

eF

old

chan

ge T

SA

/ ve

hicl

e

Fol

d ch

ange

TS

A /

vehi

cle

Fol

d ch

ange

TS

A /

vehi

cle

Fol

d ch

ange

TS

A /

vehi

cleTubulin 1a

Page 11: Supplementary figure 1

Supplementary figure 7

% o

f tra

nsfe

cted

neu

rons

with

apo

ptot

ic fe

atur

es

(fra

gmen

ted

or p

ykno

tic n

ucle

i)

0

10

20

30

40

50

GFP

p53

K-R

A.

B.

0

10

20

30

40

50

GFP

p53

K-R

% o

f tra

nsfe

cted

neu

rons

with

cle

aved

activ

e ca

spas

e 3

posi

tive

cells


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