A

B

C

3500x 8900x

5600x 14 000x

1500x 5000x

Supplementary Figure 1

TiO2

Carbon black

SWCNT

Supplementary Figure 2

DMEM

P-Iκ

BαDA

PIM

erge

SWCNT

WT p53 KO WT p53 KO

P-2κBα immunostaining of WT and p53 KO macrophages

Immunostaining quantification

Pe

rce

nta

ge

of

po

sitiv

ely

st

ain

ed

ce

lls (

%)

2µg/cm2

A

B

Supplementary figure legends

Supp Fig 1. Transmission electron microscopy images of RAW 264.7 cells incubated with 10 µg/ml (2 µg/cm2) of manufactured nanomaterials (MNMs) for 6 hr. (A) RAW 264.7 cell with TiO2 A10 MNMs within a vesicle (B) CB P60 MNMs within the cytosolic compartment of a RAW cell. (C) RAW macrophages with SWCNT at the membrane surface.

 Supp Fig 2. Consequence of p53 activation: inflammatory response in primary wild-type (WT) and p53 knock-out (KO) macrophages. (A, B) The cells were exposed to 2 µg/cm2 of TiO2 (A10), CB (FW2) and SWCNT MNMs for 30 min. The data show the percentages of phosphorylated IκBα (P- IκBα) positively stained macrophages (mean ± SEM) (n=3). * significant difference from WT control (p < 0.05). # significant difference between WT and p53 KO macrophages (p < 0.05).