5

15

25

20

10

Ct

valu

e

Log concentration

miR-375miR-221

Supplementary Figure S1

Supplementary Figure S2

T-B

il

CA

19-9

AM

Y

miR-221 concentration (amol/μl) miR-221 concentration (amol/μl)

miR-221 concentration (amol/μl)

Variables Patients (n=47) miR-221 Concentration (amol/μ ｌ ) p-value a

Age (year) mean (range) 67 (42-84)

≤65 16 ( 34.0% ) 3.31E-020.509

≥66 31 ( 66.0% ) 4.29E-02

Sex

Male 27 ( 57.4% ) 4.10E-020.508

Femal 20 ( 42.6% ) 4.29E-02

Histopathology

Adenocarcinoma 42 ( 89.4% ) 4.38E-020.359

Others 5 ( 10.6% ) 2.56E-02

Location

Ph 34 ( 72.3% ) 4.15E-020.537

Pb/Pt 13 ( 27.7% ) 3.44E-02

Depth of invasion

T0/1/2 7 ( 14.9% ) 2.87E-020.518

T3/4 40 ( 85.1% ) 4.17E-02

Lymph node metastasis

Negative 17 ( 36.2% ) 3.71E-020.233

Positive 30 ( 63.8% ) 4.27E-02

Distant metastasis

Negative 38 ( 80.9% ) 3.42E-020.023

Positive 9 ( 19.1% ) 6.08E-02

Stage

0- AⅡ 16 ( 34.0% ) 3.54E-020.117

ⅡB-Ⅳ 31 ( 66.0% ) 4.23E-02

Treatment

Resectable 37 ( 78.7% ) 3.47E-020.030

Non-resectable 10 ( 21.3% ) 5.75E-02

Characteristics and plasma miR-221 concentrations of 47 consecutive PCa patients

NOTE. Significant values are in boldface type. a P values are from the Mann-Whitney U-test and were considered significant at 0.05.

Supplementary Table S1

Supplementary Table S2

Plasma miR-221 concentration (amol/μl)

Sample n Mean 95% CI b p-value c

Pancreatic cancer 47 0.0396 0.0287-0.0505 p < 0.001

Benign tumor a 9 0.0161 0.0094-0.0227 p = 0.539

Normal control 30 0.0158 0.0113-0.0202

a Five IPMNs, 2 serous cystadenomas, and 2 pancteatitis tumors were included in benign tumors.b 95% confidential intervalc Plasma concentrations of each group were compared with those of normal controls by Mann-Whitney U test.

Differential expression pattern of plasma miR-221 in patients with pancreatic, pancreatic benign tumors, and normal controls

Plasma miR-221 Tumor miR-221

High plasma miR-221 cases a Concentration (amol/μl) Tumor expression miR-221/U6B ratio

1 1.94E-01 high 19.10

2 1.34E-01 high 1.20

3 7.16E-02 high 2.83

4 6.09E-02 high 3.39

5 5.58E-02 high 1.51

6 4.93E-02 high 1.34

Rate reflecting tumor dynamics 100% b

a Cut-off value of plasma miR-221 concentrations in healthy volunteers (mean+2SD): 4.047E-02 amol/μlb Cut-off value of miR-221/U6B ratios in normal pancreatic tissues (median): 1.00

Relationship between miRNA-221 in plasma and pancreatic cancer tissues

Supplementary Table S3