Supplemental Figure 1

Transcript of Supplemental Figure 1

TR-C2D TR-C2 TR-C2N0

40

80

120

160 SPARC/Osteonectin

#

40

80

120

160ControlCA-Akt1DN-Akt1

Fibronectin

#

# #

40

80

120

160

BSP/Osteopontin

*

#

40

80

120

160Laminin

# #

#

40

80

120

160Vitronectin

# #

*

#

(-) EGF; 12 Hr

#

CA-A

kt1

DN-A

kt1

TR-C2

pS473Akt

Cont

rol

CA-A

kt1

Cont

rol

DN-A

kt1

CA-A

kt1

Cont

rol

DN-A

kt1

TR-C2D TR-C2N

β-actin

A B C

D E F

Supplemental Figure 2

CA

-Akt

1 +

WT

β3 in

tegr

in

WT

β3 in

tegr

in

β3 integrin

β-actin

CA

-Akt

1

Con

trol

DN

-Akt

1

DN

-Akt

1 +

WT

β3 in

tegr

in

A B

CA

-Akt

1 +

DN

β3

inte

grin

DN

β3

inte

grin

CA-A

kt1

Cont

rol

β3 integrin

β-actin

0

25

50

75

10012 Hr24 Hr

#* ## ##

BSP/Osteopontin

WT

β3 in

tegr

in

CA-A

kt1

Cont

rol

DN-A

kt1

CA-A

kt 1

+

WT

β3 in

tegr

inDN

-Akt

1 +

WT

β3 in

tegr

in

C

0

25

50

75

10012 Hr24 Hr

A Vitronectin

WT

β3 in

tegr

in

CA-A

kt1

Cont

rol

DN-A

kt1

CA-A

kt 1

+

WT

β3 in

tegr

inDN

-Akt

1 +

WT

β3 in

tegr

in

#* ## ## ##

0

25

50

75

10012 Hr24 zHr SPARC/Osteonectin

** ## ## ##

WT

β3 in

tegr

in

CA-A

kt1

Cont

rol

DN-A

kt1

DN-A

kt 1

+ W

T β3

inte

grin

CA-A

kt 1

+

WT

β3 in

tegr

in

B Supplemental Figure 3

0.0 0.5 1.0 1.5 2.00.6

0.7

0.8

0.9

1.0

1.1ComtrolCA-Akt1Comtrol+WT 3b integrinCA-Akt1+WT 3b integrin

C

0.0 0.5 1.0 1.5 2.00.6

0.7

0.8

0.9

1.0

1.1ControlDN-Akt1Control+WT b3 integrinDN-Akt1+WT b3 integrin

0.7

0.8

0.9

1.0

1.1

Time (h)

0.7

0.8

0.9

1.0

1.1

D

# #

#

CA-A

kt1

+ W

T β3

inte

grin

WT

β3

inte

grin

CA-A

kt1

Cont

rol

WT

β3in

tegr

in

Cont

rol

DN-A

kt1

DN-A

kt1

+ W

T β3

inte

grin

A B Supplemental Figure 4

Supplemental Figure 1

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