Supplemental Figure 1
description
Transcript of Supplemental Figure 1
Supplemental Figure 1
TR-C2D TR-C2 TR-C2N0
40
80
120
160 SPARC/Osteonectin
#
TR-C2D TR-C2 TR-C2N0
40
80
120
160ControlCA-Akt1DN-Akt1
Fibronectin
#
# #
TR-C2D TR-C2 TR-C2N0
40
80
120
160
BSP/Osteopontin
*
#
TR-C2D TR-C2 TR-C2N0
40
80
120
160Laminin
# #
#
TR-C2D TR-C2 TR-C2N0
40
80
120
160Vitronectin
# #
*
#
(-) EGF; 12 Hr
#
CA-A
kt1
DN-A
kt1
TR-C2
pS473Akt
Cont
rol
CA-A
kt1
Cont
rol
DN-A
kt1
CA-A
kt1
Cont
rol
DN-A
kt1
TR-C2D TR-C2N
β-actin
A B C
D E F
Supplemental Figure 2
CA
-Akt
1 +
WT
β3 in
tegr
in
WT
β3 in
tegr
in
β3 integrin
β-actin
CA
-Akt
1
Con
trol
DN
-Akt
1
DN
-Akt
1 +
WT
β3 in
tegr
in
A B
CA
-Akt
1 +
DN
β3
inte
grin
DN
β3
inte
grin
CA-A
kt1
Cont
rol
β3 integrin
β-actin
0
25
50
75
10012 Hr24 Hr
#* ## ##
BSP/Osteopontin
WT
β3 in
tegr
in
CA-A
kt1
Cont
rol
DN-A
kt1
CA-A
kt 1
+
WT
β3 in
tegr
inDN
-Akt
1 +
WT
β3 in
tegr
in
C
0
25
50
75
10012 Hr24 Hr
A Vitronectin
WT
β3 in
tegr
in
CA-A
kt1
Cont
rol
DN-A
kt1
CA-A
kt 1
+
WT
β3 in
tegr
inDN
-Akt
1 +
WT
β3 in
tegr
in
#* ## ## ##
0
25
50
75
10012 Hr24 zHr SPARC/Osteonectin
** ## ## ##
WT
β3 in
tegr
in
CA-A
kt1
Cont
rol
DN-A
kt1
DN-A
kt 1
+ W
T β3
inte
grin
CA-A
kt 1
+
WT
β3 in
tegr
in
B Supplemental Figure 3
0.0 0.5 1.0 1.5 2.00.6
0.7
0.8
0.9
1.0
1.1ComtrolCA-Akt1Comtrol+WT 3b integrinCA-Akt1+WT 3b integrin
C
0.0 0.5 1.0 1.5 2.00.6
0.7
0.8
0.9
1.0
1.1ControlDN-Akt1Control+WT b3 integrinDN-Akt1+WT b3 integrin
0.7
0.8
0.9
1.0
1.1
Time (h)
Time (h)
0.7
0.8
0.9
1.0
1.1
D
# #
#
#
CA-A
kt1
+ W
T β3
inte
grin
WT
β3
inte
grin
CA-A
kt1
Cont
rol
WT
β3in
tegr
in
Cont
rol
DN-A
kt1
DN-A
kt1
+ W
T β3
inte
grin
A B Supplemental Figure 4