Supplementary Figure 1.
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Supplementary Figure 1. Supplementary Figure 1: (A) Histograms showing cellular expression levels of TNF, IL-1, SOD2 and cMyc in cells exposed to 10, 50 or 100cGy radiation and harvested after 12, 24 and 48h. Densitometry analysis was performed using ImageQuant 1D gel analysis software and the band volume data were normalized to corresponding α-tubulin band volume. The normalized values were then compared to mock-IR controls and were plotted as percent baseline (mock-IR). (B) Histograms showing cellular expression levels of TNF, IL-1, SOD2, pIBα and cMyc in cells either mock-irradiated, exposed to CDIR (4Gy), co-cultured with LDIR (10, 50 or 100cGy) treated cells followed by CDIR exposure and harvested after 48 and 72h. Co-culturing with LDIR-exposed cells significantly increased cellular levels of TNF, IL-1, SOD2 and cMyc in bystander Mock-IR SOD2 MYC IL-1a TNFa 0 200 300 400 500 Mock-IR ANOVA P<0.001 N otSignificant ‡ ‡ ‡ ‡ ‡ ‡ ‡ ‡ ‡ ‡ ‡ ‡ ‡ ‡ ‡ ‡ ‡ ‡ ‡ ‡ ‡ ‡ ‡ ‡ ‡ ‡ ‡ ‡ ‡ ‡ ‡ ‡ ‡ ‡ ‡ * -tubulin norm alized 0.1Gy-12h 0.1Gy-24h 0.1Gy-48h 0.5Gy-12h 0.5Gy-24h 0.5Gy-48h 1.0Gy-12h 1.0Gy-24h 1.0Gy-48h % B aseline (M ock-IR )* Mock-IR TNF SOD2 IL-1 pI B MYC 0 200 300 400 500 Mock-IR ANOVA P<0.001 N otSignificant ‡ ‡ ‡ ‡ ‡ ‡ ‡ ‡ ‡ ‡ ‡ ‡ ‡ ‡ ‡ ‡ ‡ ‡ ‡ ‡ ‡ ‡ ‡ * -tubulin norm alized C DIR -48h C DIR -72h 0.1G y+CDIR-48h 0.1G y+CDIR-72h 0.5G y+CDIR-48h 0.5G y+CDIR-72h 1.0G y+CDIR-48h 1.0G y+CDIR-72h ‡ ‡ ‡ ‡ ‡ ‡ ‡ ‡ ‡ % B aseline (M ock-IR )* (A) (B)
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Supplementary Figure 1.
Supplementary Figure 1: (A) Histograms showing cellular expression levels of TNF, IL-1, SOD2 and cMyc in cells exposed to 10, 50 or 100cGy radiation and harvested after 12, 24 and 48h. Densitometry analysis was performed using ImageQuant 1D gel analysis software and the band volume data were normalized to corresponding α-tubulin band volume. The normalized values were then compared to mock-IR controls and were plotted as percent baseline (mock-IR). (B) Histograms showing cellular expression levels of TNF, IL-1, SOD2, pIBα and cMyc in cells either mock-irradiated, exposed to CDIR (4Gy), co-cultured with LDIR (10, 50 or 100cGy) treated cells followed by CDIR exposure and harvested after 48 and 72h. Co-culturing with LDIR-exposed cells significantly increased cellular levels of TNF, IL-1, SOD2 and cMyc in bystander cells.
Mock-IR
SOD2 MYC IL-1a TNFa0
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Mock-IR
ANOVA P<0.001 Not Significant
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* -tubulin normalized
0.1Gy-12h0.1Gy-24h0.1Gy-48h
0.5Gy-12h0.5Gy-24h0.5Gy-48h
1.0Gy-12h1.0Gy-24h1.0Gy-48h
% B
asel
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(Mo
ck-I
R)*
Mock-IR
TNF SOD2 IL-1 pIB MYC0
200
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Mock-IR
ANOVA P<0.001 Not Significant
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* -tubulin normalized
CDIR-48hCDIR-72h
0.1Gy+CDIR-48h0.1Gy+CDIR-72h
0.5Gy+CDIR-48h0.5Gy+CDIR-72h
1.0Gy+CDIR-48h1.0Gy+CDIR-72h
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% B
asel
ine
(Mo
ck-I
R)*
(A)
(B)
