REPRESSILATORSILAS FERRAO

WHAT IS IT?

IN 2000 STANISLAS LIEBLER AND MICHAEL ELOWITZ CREATED GENETIC MACHINE

CALLED REPRESSILATOR-MILESTONE IN SYNTHETIC BIOLOGY, IT WAS AN OSCILLATORY GENTIC MACHINE

USED E. COLI TO EXHIBIT REGULATORY NETWORK

HOW DOES IT WORK?

“GENETIC CLOCK”

TETR, LACL, λCL ARE REPRESSORS

PTET, PLAC, λPR ARE PROMOTERS

ARRANGED ON PLASMID ALONGSIDE GFP PLASMID

HOW DOES IT WORK?

L-PRLACL

PROBLEMS…..

OSCILLATIONS LAST FOR TWO AND A HALF HOURS

LOSE SYNCH WITHIN SYSTEM

SYSTEM IS “NOISY”

DEVICE….

CELIAC DISEASE

I HATE IT WHEN THIS HAPPENS TO ME…

WHAT IS IT?

AUTOIMMUNE DISORDER IN SMALL INTESTINE

DAMAGES THE VILLI (ATROPHY)

REACTION TO GLIADIN

GLIADIN

GROUP OF PROTEIN FOUND IN GLUTEN

A-GLIADIN

GLUTEN HALF OF THE WHEAT SEED

DEVICE

Chromosome

Plasmid

CXCR3Cyclic AMP CR

E. COLI

H. PYLORI

A-gliadin promoter

HD RBSORF

RHO TERM.

GLN BPR2-HMW

HD RBS

RESULTS….

VERY IMPRACTICAL

GLUTEN ANGLE

RECEPTORS, TERMINATORS, BACTERIA ONLY TO DEAL WITH INHBITING GLUTEN CROSSLINKING

THANK YOU DOE!