(a)
2
A1,2: Positive control B1,2: --- C1,2: --- D1,2: --- E1,2: Positive control A3,4: C5a B3,4: IL-1a C3,4: IL-10 D3,4: IL-32α E3,4: RANTES A5,6: CD40 ligand B5,6: IL-1β C5,6: IL-12 p70 D5,6: CXCL10 E5,6: CXCL12/SDF-1 A7,8: G-CSF B7,8: IL-1ra C7,8: IL-13 D7,8: CXCL11 E7,8: TNF-α A9,10: GM-CSF B9,10: IL-2 C9,10: IL-16 D9,10: MCP-1 E9,10: sTREM-1 A11,12: GRO-α B11,12: IL-4 C11,12: IL-17 D11,12: MIF E11,12: --- A13,14: CCL1/I-309 B13,14: IL-5 C13,14: IL-17E D13,14: MIP-1α E13,14: --- A15,16: sICAM-1 B15-16: IL-6 C15,16: IL-23 D15,16: MIP-1β E15,16: --- A17,18: IFN-γ B17,18: IL-8 C17,18: IL-27 D17,18: PAI-1 E17,18: --- A19-20: Positive control B19,20: --- C19,20: --- D19-20: --- E19,20: Negative control (a ) A B C D E 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 Cytokine Array
description
(a). Cytokine Array. 1 2. 3 4. 5 6. 7 8. 9 10. 11 12. 13 14. 15 16. 17 18. 19 20. A. B. C. D. E. (b). Angiogenesis Array. 1 2. 3 4. 5 6. 7 8. 9 10. 11 12. 13 14. 15 16. 17 18. 19 20. 21 22. 23 24. A. B. C. D. E. F. Supplementary Figure 1: - PowerPoint PPT Presentation
Transcript of (a)
A1,2: Positive control
B1,2: ---
C1,2: ---
D1,2: ---
E1,2: Positive control
A3,4: C5a
B3,4: IL-1a
C3,4: IL-10
D3,4: IL-32α
E3,4: RANTES
A5,6: CD40 ligand
B5,6: IL-1β
C5,6: IL-12 p70
D5,6: CXCL10
E5,6: CXCL12/SDF-1
A7,8: G-CSF
B7,8:IL-1ra
C7,8: IL-13
D7,8: CXCL11
E7,8: TNF-α
A9,10: GM-CSF
B9,10: IL-2
C9,10: IL-16
D9,10: MCP-1
E9,10: sTREM-1
A11,12: GRO-α
B11,12: IL-4
C11,12: IL-17
D11,12: MIF
E11,12: ---
A13,14: CCL1/I-309
B13,14: IL-5
C13,14: IL-17E
D13,14: MIP-1α
E13,14: ---
A15,16: sICAM-1
B15-16: IL-6
C15,16: IL-23
D15,16: MIP-1β
E15,16: ---
A17,18: IFN-γ
B17,18: IL-8
C17,18: IL-27
D17,18: PAI-1
E17,18: ---
A19-20: Positive control
B19,20: ---
C19,20: ---
D19-20: ---
E19,20: Negative control
(a)
ABCDE
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20
Cytokine Array
(b)
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20
A
BCDE
F
21 22 23 24
A1,2: Positive control
B1,2: TF
C1,2:GDNF
D1,2:MIP-1a
E1,2:Serpin B5
F1,2:Positive control
A3,4: ---
B3,4: CXCL16
C3,4:GM-CSF
D3,4:MMP-8
E3,4:PAI-1
F3,4:---
A5,6: Activin A
B5,6: CD26
C5,6:HB-EGF
D5,6:MMP-9
E5,6:Serpin F1
F5,6:---
A7,8: ADAMTS-1
B7,8: EGF
C7,8:HGF
D7,8:NRG1-β1
E7,8:TIMP-1
F7,8:---
A9,10: Angiogenin
B9,10: EG-VEGF
C9,10:IGFBP-1
D9,10:Pentraxin 3
E9,10:TIMP-4
F9,10:---
A11,12: Angiopoietin-1
B11,12: CD105
C11,12:IGFBP-2
D11,12:PD-ECGF
E11,12:TSP-1
F11,12:---
A13,14: Angiopoietin-2
B13,14: Endostatin
C13,14:IGFBP-3
D13,14:PDGF-AA
E13,14:TSP-2
F13,14:---
A15,16: Plasminogen
B15,16: Endothelin-1
C15,16:IL-1β
D15,16:PDGF-AB/BB
E15,16:uPA
F15,16:---
A17,18: Amphiregulin
B17,18: FGF acidic
C17,18:IL-8
D17,18:Persephin
E17,18:Vasohibin
F17,18:---
A19,20: Artemin B19-20:FGF basic
C19,20:TGF-β1
D19,20:CXCL4
E19,20:VEGF
F19,20:---
A21,22: ---
B21,22:FGF-4
C21,22: Leptin
D21,22:PlGF
E21,22:VEGF-C
F21,22:---
A23,24: Positive control
B23,24:FGF-7
C23,24: MCP-1
D23,24:Prolactin
E23,24:---
F23,24:Negative control
Angiogenesis Array
Supplementary Figure 1:
Membrane array analysis of APOSECH. Membrane arrays were utilized to scan the protein content of APOSECH for various (a) cytokines and (b) angiogenic factors.
Supplementary Figure 1 Methods
Membrane arrays for the detection of cytokines and angiogenic factors in cell culture supernatants derived from apoptotic PBMC were performed to scan the protein content of APOSEC (Proteome Profiler Array Ary005 and Ary007, R&D Systems, USA). Experiments were performed according to the manufacturer’s protocol.
