11103_2011_9797_MOESM2_ESM
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0 10000 20000 30000 40000 50000 60000 70000 80000 90000 12 13 14 15 16 17 18 19 20 21 22 23 24 fraction num ber band volum e (AU ) G roEL G roES β1 Fig S1:Elution profile of GroEL, GroES, β1 reconstitution mixture from Superdex 200 gel filtration column: GroEL (1 mg) and GroES (1 mg) or reconstitution mixture containing β1 (0.75 mg total) was injected into a Superdex 200 gel filtration column in Tris buffer pH 8.0 and run a rate of 1 ml/min for 120 min. Fractions of 3 ml were collected, run on an SDS-PAGE minigel and quantified by densitometry.
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fraction number
ba
nd
vo
lum
e (
AU
)GroEL
GroES
β1
Fig S1:Elution profile of GroEL, GroES, β1 reconstitution mixture from Superdex 200 gel filtration column: GroEL (1 mg) and GroES (1 mg) or reconstitution mixture containing β1 (0.75 mg total) was injected into a Superdex 200 gel filtration column in Tris buffer pH 8.0 and run a rate of 1 ml/min for 120 min. Fractions of 3 ml were collected, run on an SDS-PAGE minigel and quantified by densitometry.
