Supplementary Table 1 for Fig. 1.

OP-to-BI

ratio

Exp.

No.

Mean life spanMaximum life

span

No. of

nematodesLog-rank test

± SE (days old) ± SE (days old) (No. of lost

worms)p value

OP (control)

1

12.37 ± 0.49 17.23 ± 0.24 76 (1)

1:1 13.88 ± 0.46 19.12 ± 0.38 86 (4) 0.01184

2:3 14.03 ± 0.44 19.10 ± 0.27 98 (2) 0.00268

3:7 14.52 ± 0.49 20.29 ± 0.28 93 (1) 0.00011

1:4 15.16 ± 0.45 19.25 ± 0.28 83 (4) 0.00001

OP (control)

2

13.40 ± 0.44 18.20 ± 0.26 68 (1)

1:1 14.02 ± 0.48 18.70 ± 0.25 67 (3) 0.17321

2:3 14.41 ± 0.58 20.70 ± 0.49 68 (1) 0.01501

3:7 14.34 ± 0.52 18.83 ± 0.33 62 (3) 0.06154

1:4 14.99 ± 0.45 19.68 ± 0.40 71 (3) 0.00706

OP (control)

3

13.39 ± 0.45 18.05 ± 0.55 73 (0)

1:1 13.62 ± 0.43 17.50 ± 0.30 68 (1) 0.93016

3:7 14.46 ± 0.45 18.59 ± 0.44 71 (0) 0.10860

1:4 14.82 ± 0.49 19.77 ± 0.41 73 (3) 0.01125

OP (control)

4

12.81 ± 0.49 17.50 ± 0.19 71 (0)

1:1 14.50 ± 0.50 19.14 ± 0.31 70 (2) 0.00375

3:7 14.73 ± 0.44 19.23 ± 0.27 73 (1) 0.00354

1:4 14.68 ± 0.46 19.14 ± 0.20 72 (1) 0.00337

- 1 -

OP (control)

5

13.75 ± 0.50 18.39 ± 0.20 63 (1)

3:7 15.25 ± 0.66 20.61 ± 0.35 59 (1) 0.00141

1:4 15.85 ± 0.49 19.94 ± 0.29 62 (0) 0.00099

OP (control)

6

13.50 ± 0.42 17.72 ± 0.52 59 (1)

3:7 16.22 ± 0.49 20.94 ± 0.41 58 (2) 0.00004

1:4 16.10 ± 0.54 21.06 ± 0.38 60 (0) 0.00001

- 2 -

Supplementary Table 2 for Fig. 8a.

OP-to-BI

ratio

Exp.

No.

Mean life span Max. life spanNo. of

nematodesLog-rank test

± SE (days old) ± SE (days old) (No. of lost

worms)p value

OP (control)

1

15.50 ± 0.42 19.68 ± 0.18 76 (0)

0:10

(whole cell)20.55 ± 0.42 24.95 ± 0.28 75 (3) 6.26472E-14

1:4

(whole cell)17.57 ± 0.48 22.23 ± 0.24 76 (0) 1.49111E-05

1:4

(cell wall)17.84 ± 0.53 22.20 ± 0.30 64 (1) 5.30747E-06

OP (control)

2

13.62 ± 0.53 18.90 ± 0.31 68 (2)

0:10

(whole cell)18.43 ± 0.57 24.20 ± 0.47 67 (3)

1.00742E-

09

1:4

(whole cell)15.75 ± 0.49 20.80 ± 0.37 69 (1) 0.00464

1:4

(cell wall)16.78 ± 0.51 22.10 ± 0.52 69 (1)

2.84866E-

05

OP (control) 3 14.30 ± 0.62 20.86 ± 0.45 70 (0)0:10

(whole cell) 18.25 ± 0.71 25.50 ± 0.42 67 (3) 7.26630E-06

1:4 17.33 ± 0.56 22.80 ± 0.58 69 (1) 0.00075

- 3 -

(whole cell)

1:4

(cell wall) 17.35 ± 0.59 23.30 ± 0.47 68 (2) 0.00052

- 4 -

Supplementary Table 3 for Fig. 8b.

OP-to-BI

ratio

Exp.

No.

Mean life span Max. life spanNo. of

nematodesLog-rank test

± SE (days old) ± SE (days old) (No. of lost

worms)p value

OP (control)

1

13.45 ± 0.48 18.83 ± 0.47 60 (2)

0:10

(whole cell)16.20 ± 0.55 21.25 ± 0.25 54 (8) 0.00029

0:10

(protoplast)16.75 ± 0.47 20.83 ± 0.17 60 (1) 0.00001

OP (control)

2

13.98 ± 0.50 19.10 ± 0.48 66 (4)0:10

(whole cell) 16.74 ± 0.67 23.61 ± 0.48 63 (7) 1.47589E-04

0:10

(protoplast) 17.28 ± 0.64 23.10 ± 0.37 64 (6) 3.73129E-06

OP (control)

3

14.80 ± 0.54 20.77 ± 0.33 74 (0)0:10

(whole cell) 19.15 ± 0.62 24.20 ± 0.21 66 (8) 2.64293E-08

0:10

(protoplast) 19.61 ± 0.62 23.50 ± 0.19 54 (18) 7.62211E-09

- 5 -

Supplementary Table 4 for Fig. 9a.

MutantsExp.

No.

Mean life span Max. life spanNo. of

nematodesLog-rank test

± SE (days old) ± SE (days old) (No. of lost

worms)p value

Δ pmk-1 OP1

12.26 ± 0.58 17.83 ± 0.44 58 (2)0.05244

Δ pmk-1 BI 13.27 ± 0.75 20.38 ± 0.30 53 (7)

Δ pmk-1 OP2

12.65 ± 0.56 17.61 ± 0.45 62 (1)0.06013

Δ pmk-1 BI 12.92 ± 0.78 19.75 ± 0.31 52 (8)

- 6 -

Supplementary Table 5 for Fig. 9b.

MutantsExp.

No.

Mean life span Max. life spanNo. of

nematodesLog-rank test

± SE (days old) ± SE (days old) (No. of lost

worms)p value

Δ skn-1 OP 1

15.37 ± 0.53 19.86 ± 0.28 75 (0)0.76058

Δ skn-1 BI 15.60 ± 0.51 20.30 ± 0.33 68 (6)

Δ skn-1 OP 2

12.99 ± 0.34 17.57 ± 0.30 97 (1)0.47597

Δ skn-1 BI 13.49 ± 0.35 18.00 ± 0.53 82 (17)

- 7 -

Supplementary Table 6 for Fig. 9c.

MutantsExp.

No.

Mean life span Max. life spanNo. of

nematodesLog-rank test

± SE (days old) ± SE (days old) (No. of lost

worms)p value

Δ vhp-1 OP1

11.41 ± 0.30 14.06 ± 0.24 58 (3)0.03469

Δ vhp-1 BI 12.39 ± 0.31 15.50 ± 0.27 56 (4)

Δ vhp-1 OP 2

11.28 ± 0.38 15.17 ± 0.29 59 (1)0.01460

Δ vhp-1 BI 12.88 ± 0.32 15.94 ± 0.18 58 (2)

- 8 -

Supplementary Table 7 for Fig. 9d

MutantsExp.

No.

Mean life span Max. life spanNo. of

nematodesLog-rank test

± SE (days old) ± SE (days old) (No. of lost

worms)p value

Δ tol-1 OP

(control) 116.45 ± 0.61 22.61 ± 0.56 61 (1)

2.60178E-13

Δ tol-1 BI 25.56 ± 0.97 32.63 ± 0.55 54 (7)

Δ tol-1 OP

(control) 216.98 ± 0.65 22.25 ± 0.25 56 (5)

5.15364E-08

Δ tol-1 BI 22.06 ± 1.03 30.38 ± 0.48 55 (5)

- 9 -

Supplementary Table 8 for Fig. 9e.

MutantsExp.

No.

Mean life span Max. life spanNo. of

nematodesLog-rank test

± SE (days old) ± SE (days old) (No. of lost

worms)p value

Δ tir-1 OP 1

18.73 ± 0.80 25.75 ± 0.56 53 (7)0.00102

Δ tir-1 BI 22.33 ± 0.98 29.50 ± 0.73 41 (19)

Δ tir-1 OP 2

17.64 ± 0.70 24.06 ± 0.38 58 (2)0.00861

Δ tir-1 BI 21.29 ± 1.08 30.25 ± 0.85 28 (32)

Δ tir-1 OP 3

17.01 ± 0.81 24.38 ± 0.61 55 (5)2.45191E-05

Δ tir-1 BI 21.97 ± 1.11 29.50 ± 0.82 38 (22)

- 10 -

Supplementary Table 9 for Fig. 10a.

MutantsExp.

No.

Mean life span Max. life spanNo. of

nematodesLog-rank test

± SE (days old) ± SE (days old) (No. of lost

worms)p value

Δ daf-16 OP 1

12.83 ± 0.46 16.80 ± 0.15 64 (1)3.35175E-

11Δ daf-16 BI 18.20 ± 0.62 23.63 ± 0.40 56 (7)

Δ daf-16 OP 2

13.48 ± 0.51 18.38 ± 0.40 56 (4)7.81899E-

09Δ daf-16 BI 19.63 ± 0.95 25.33 ± 0.65 40 (20)

- 11 -

Supplementary Table 10 for Fig. 10b.

MutantsExp.

No.

Mean life span Max. life spanNo. of

nematodesLog-rank test

± SE (days old) ± SE (days old) (No. of lost

worms)p value

Δ daf-2 OP 1

21.56 ± 0.59 28.38 ± 0.31 104 (1)0.45249

Δ daf-2 BI 22.97 ± 0.48 29.43 ± 0.53 97 (10)

Δ daf-2 OP 2

25.87 ± 0.85 36.10 ± 0.85 65 (0)0.02491

Δ daf-2 BI 28.32 ± 1.13 39.25 ± 0.67 56 (6)

- 12 -

Supplementary Table 11 for Fig. 10c.

MutantsExp.

No.

Mean life span Max. life spanNo. of

nematodesLog-rank test

± SE (days old) ± SE (days old) (No. of lost

worms)p value

Δ jnk-1 OP 1

15.25 ± 0.58 21.06 ± 0.34 63 (0)2.55585E-08

Δ jnk-1 BI 21.31 ± 0.89 29.50 ± 0.57 54 (5)

Δ jnk-1 OP2

16.43 ± 0.56 21.72 ± 0.46 58 (2)0.00013

Δ jnk-1 BI 18.64 ± 1.05 27.94 ± 0.69 57 (3)

- 13 -

Supplementary Table 12 for Fig. 10d.

MutantsExp.

No.

Mean life span Max. life spanNo. of

nematodesLog-rank test

± SE (days old) ± SE (days old) (No. of lost

worms)p value

Δaak-2 OP

(control) 113.54 ± 0.63 19.72 ± 0.32 57 (3) 6.12868E-

07Δaak-2 BI 18.22 ± 0.84 24.07 ± 0.43 46 (13)

Δaak-2 OP

(control) 214.83 ± 0.54 20.50 ± 0.60 57 (4) 3.52659E-

06Δaak-2 BI 18.77 ± 0.73 24.21 ± 0.18 49 (11)

- 14 -