TOMM40 ‘523 variant and cognitive decline in older persons with APOE ε3/3 genotype

SUPPLEMENTAL METHODS

Tests for annual cognitive assessment

Uniform and structured cognitive testing was administered each year via a battery of 17 tests.

Five cognitive domains were assessed. Specifically, episodic memory was assessed using 7 tests,

which include immediate and delayed recall of story A from the logical memory subtest of the

Wechsler Memory Scale-Revised, immediate and delayed recall of the East Boston story, word

list memory, word list recall, and word list recognition. Semantic memory was assessed using 3

tests, including Boston naming, national adult reading and category fluency tests. Working

memory was assessed using 3 tests, including digit span forward, digit span backward and digit

ordering. Perceptual speed was assessed using 2 tests, including symbol digit modalities and

number comparison. Finally, visuospatial ability was assessed using 2 tests, including judgment

of line orientation and standard progressive matrices. Raw scores on each test were standardized

using the baseline mean and standard deviation of the entire cohorts. Individual standardized test

scores were averaged to obtain composite measures of global cognition and individual cognitive

domains. A higher score indicates better cognitive performance.

Figure e-1

Figure e-1 shows the histograms of the length (number of T residues) for each TOMM40 ‘523 allele among APOE ε3/3 carriers. The vertical dotted lines reflect the cut-offs used to determine the ‘523 categorical genotypes (S, L and VL).