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BIOL20332/20972 GENETICS / Dev. Biol. RSM MODULE 3. transposable elements. Making use of transposons !!!!. Transposons as virus-like parasitic elements?. Analysing gene regulation. wt stained with anti-slit. Analysing gene regulation. fly. molecular cloning. Analysing gene regulation. - PowerPoint PPT Presentation

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  • transposableelementsBIOL20332/20972GENETICS / Dev. Biol. RSMMODULE 3

  • Transposons as virus-like parasitic elements?Making use of transposons!!!!

  • wt stained with anti-slitAnalysing gene regulation

  • Analysing gene regulationflymolecularcloning

  • Analysing gene regulation

  • Analysing gene regulation

  • wt stained with anti-slitslit-lacZ stained with anti-GalAnalysing gene regulation

  • Groupslarval genotypestainingcommentsA B C DA-lacZ2hr 37C, then o.N. 26C4 groupsE F G HB-lacZ2hr 37C4 groupsI J K LC-lacZ2hr 26C4 groupsM N OD-lacZ2hr 37C, then o.N. 26C3 groupsP Q RE-Gal4;UAS-lacZ2hr 37C3 groups, stock a bit weakS T UF-Gal4;UAS-lacZ2hr 37C3 groupsV W X YG-lacZ2hr 37C, then o.N. 26C4 groups

  • How to generate transgenic fly lines with these constructs?

  • Making transgenic flies

  • Making transgenic flies

  • Important improvements of transposable elements that have shaped work with Drosophila

  • PEnhancer trap experiments

  • Enhancer trap experimentsDifference: enhancer is not transgenic but endogenous

  • Rescue experiment of enhancer trap lines to clone the adjacent gene

  • The Gal4/UAS system

  • X-Gal4 ; UAS-lacZ

  • Groupslarval genotypestainingcommentsA B C DA-lacZ2hr 37C, then o.N. 26C4 groupsE F G HB-lacZ2hr 37C4 groupsI J K LC-lacZ2hr 26C4 groupsM N OD-lacZ2hr 37C, then o.N. 26C3 groupsP Q RE-Gal4;UAS-lacZ2hr 37C3 groups, stock a bit weakS T UF-Gal4;UAS-lacZ2hr 37C3 groupsV W X YG-lacZ2hr 37C, then o.N. 26C4 groups

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