SurePlex DNA Amplification System - BlueGnome Ltd · Product Information Sheet: Reproductie and...
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Product Information Sheet: Reproductive and Genetic Health Description The SurePlex DNA Amplification System reproducibly amplifies total DNA from single cells or a few cells to produce 2–5 μg of amplified DNA in 2 hours. The SurePlex system is intended for use within 24sure™ protocols, before labeling and hybridization to 24sure or 24sure+ microarrays, and for use in VeriSeq preimplantation genetic screening (PGS) applications. Supply The SurePlex kit is supplied in a single pack, suitable for 50 amplification reactions, and is shipped on dry ice. For detailed information on the SurePlex DNA Amplification System, see the Contents section. The SurePlex DNA Amplification System consists of eight components, including the enzymes and buffers required to perform the amplification protocol. The contents are provided in clear screw- capped tubes with color-coded lids referred to in the SurePlex laboratory protocol. The SurePlex Amplification System pack includes a protocol insert. Each pack is labeled with the product name and model number, lot number, storage conditions, and expiration date. Storage Upon receipt, the SurePlex DNA Amplification System should be stored at -20°C in a non–frost-free freezer until required. The SurePlex DNA Amplification system should be used before the expiration date. Technical Specifications The SurePlex DNA Amplification System uses semi-random, non–self- complementary primers to make a library of DNA sequences that can be amplified > 1,000-fold. The kit employs a universal-primer PCR to produce DNA of a standard concentration that can then be used for a downstream application, such as microarray hybridization. Quality Control Each lot of SurePlex kits is performance-tested for quality assurance. Female genomic DNA is amplified using the SurePlex protocol and reaction products quantified by agarose gel electrophoresis. Absence of reagent contamination is checked using negative-control reactions. The amplified products are labeled and analyzed in two 24sure sex-mismatched hybridizations. The spread of data points on the autosomal chromosomes, and the shift from 0 (log 2 scale) of data points on the sex chromosomes are used to determine performance. To make sure that components of the SurePlex Amplification System are supplied with the correct quantities, three kits from each batch are checked for reagent fill volumes. Contents See Table 1 for a complete list of kit contents and part numbers. Safety Information Safety data sheets are available for download from www.cambridgebluegnome.com. PR-40-415101-00: SurePlex DNA Amplification System Additional Documents Additional documents available for download include: • Laboratory Reference Manuals: 24sure Dual Channel, 24sure Single Channel, SurePlex • Laboratory Summary Protocols: 24sure Dual Channel, 24sure Single Channel, SurePlex Notices SurePlex contains technology developed and manufactured by Rubicon Genomics Inc., Ann Arbor, Michigan, USA. United States and international patents pending SurePlex ™ DNA Amplification System Produce 2–5 μg DNA from a few cells.
Transcript of SurePlex DNA Amplification System - BlueGnome Ltd · Product Information Sheet: Reproductie and...
Product Information Sheet: Reproductive and Genetic Health
Description
The SurePlex DNA Amplification System reproducibly amplifies total DNA from single cells or a few cells to produce 2–5 μg of amplified DNA in 2 hours. The SurePlex system is intended for use within 24sure™ protocols, before labeling and hybridization to 24sure or 24sure+ microarrays, and for use in VeriSeq preimplantation genetic screening (PGS) applications.
Supply
The SurePlex kit is supplied in a single pack, suitable for 50 amplification reactions, and is shipped on dry ice. For detailed information on the SurePlex DNA Amplification System, see the Contents section.
The SurePlex DNA Amplification System consists of eight components, including the enzymes and buffers required to perform the amplification protocol. The contents are provided in clear screw-capped tubes with color-coded lids referred to in the SurePlex laboratory protocol.
The SurePlex Amplification System pack includes a protocol insert. Each pack is labeled with the product name and model number, lot number, storage conditions, and expiration date.
Storage
Upon receipt, the SurePlex DNA Amplification System should be stored at -20°C in a non–frost-free freezer until required.
The SurePlex DNA Amplification system should be used before the expiration date.
Technical Specifications
The SurePlex DNA Amplification System uses semi-random, non–self-complementary primers to make a library of DNA sequences that can be amplified > 1,000-fold. The kit employs a universal-primer PCR to produce DNA of a standard concentration that can then be used for a downstream application, such as microarray hybridization.
Quality Control
Each lot of SurePlex kits is performance-tested for quality assurance. Female genomic DNA is amplified using the SurePlex protocol and reaction products quantified by agarose gel electrophoresis. Absence of reagent contamination is checked using negative-control reactions. The amplified products are labeled and analyzed in two 24sure sex-mismatched hybridizations. The spread of data points on the autosomal chromosomes, and the shift from 0 (log2 scale) of data points on the sex chromosomes are used to determine performance.
To make sure that components of the SurePlex Amplification System are supplied with the correct quantities, three kits from each batch are checked for reagent fill volumes.
Contents
See Table 1 for a complete list of kit contents and part numbers.
Safety Information
Safety data sheets are available for download from www.cambridgebluegnome.com.
PR-40-415101-00: SurePlex DNA Amplification System
Additional Documents
Additional documents available for download include:
• Laboratory Reference Manuals: 24sure Dual Channel, 24sure Single Channel, SurePlex
• Laboratory Summary Protocols: 24sure Dual Channel, 24sure Single Channel, SurePlex
Notices
SurePlex contains technology developed and manufactured by Rubicon Genomics Inc., Ann Arbor, Michigan, USA. United States and international patents pending
SurePlex™ DNA Amplification SystemProduce 2–5 μg DNA from a few cells.
Product Information Sheet: Reproductive and Genetic Health
Illumina • 1.800.809.4566 toll-free (U.S.) • +1.858.202.4566 tel • [email protected] • www.illumina.com
FOR RESEARCH USE ONLY
© 2014 Illumina, Inc. All rights reserved.Illumina, SurePlex, 24sure, the pumpkin orange color, and the Genetic Energy streaming bases design are trademarks or registered trademarks of Illumina, Inc. All other brands and names contained herein are the property of their respective owners. Pub. No. 1570-2014-017 Current as of 07 August 2014
Table 1: SurePlex DNA Amplification System
Catalog No. Description No. Rxns Item No. Description Size
PR-40-415101-00 SurePlex DNA Amplification System 50 R30050-01 Cell Extraction Buffer 300 µl
R30050-02 Extraction Enzyme Dilution Buffer
300 µl
R30050-03 Cell Extraction Enzyme 15 µl
R30050-04 SurePlex Pre-Amp Buffer 275 µl
R30050-05 SurePlex Pre-Amp Enzyme 15 µl
R30050-06 SurePlex Amplification Buffer 1.4 ml
R30050-07 SurePlex Amplification Enzyme
50 µl
R30050-08 Nuclease-Free Water 1.8 ml
Ordering Information
Product Catalog No.
SurePlex DNA Amplification System (50 reactions)
PR-40-415101-00
![Nucleosid * DNA polymerase { ΙΙΙ, Ι } * Nuclease { endonuclease, exonuclease [ 5´,3´ exonuclease]} * DNA ligase * Primase.](https://static.fdocument.org/doc/165x107/56649cab5503460f9496ce53/nucleosid-dna-polymerase-nuclease-endonuclease-exonuclease.jpg)
