Putative DNA-binding domain

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TT. TT. T. T. Putative DNA-binding domain. TT. Helix. T. Turn. Figure S1. A. KDa. 100. 72. 55. 40. 33. 24. 17. VqsM t -6His ( μ M). B. C. 11. 0.1. 0.2. 0.5. 0.1. 0.2. 0.5. 1.0. 1.0. 1 2. 0. 0. rhlI -p. vqsR -p. Figure S2. - PowerPoint PPT Presentation

Transcript of Putative DNA-binding domain

1

TTTTTTTTPutative DNA-binding domainTHelixTurnFigure S1Figure S2

rhlI-pvqsR-p0.51.00.20.100.51.00.20.10VqsMt-6His (M)ABC

55172433407210011KDa1 2Protein namePosition(Start to End)Protein name

Position(Start to End)VqsMt1WRPLQ to RNSFH VqsMt6FHVNV to RNSFHVqsMt2PFAAE to RNSFH VqsMt7HEGND to RNSFHVqsMt3VIGLA to RNSFHVqsMt8PAHQL to RRWTG VqsMt4IRAYV to RNSFHVqsMt9PAVFQ to RRWTGVqsMt5EVEIH to RNSFHVqsMtPAHQL to RNSFHFigure S34 hr 12 hr 24 hr

lasI-P100T/ClasI-P99G/TlasI-P98A/GlasI-P97T/GlasI-P96C/AVqsMt-6His (M)Free DNAProtein- DNA0.51.00.20.100.51.00.20.100.51.00.20.100.51.00.20.10Free DNAProtein- DNAlasI-P95T/GlasI-P94T/CVqsMt-6His (M)Free DNAProtein- DNAlasI-P93T/GlasI-P92T/AlasI-P91C/AlasI-P90G/TlasI-P89G/A0.51.00.20.100.51.00.20.100.51.00.20.100.51.00.20.100.51.00.20.10Figure S4lasI-P88A/TFree DNAProtein- DNAlasI-P87C/A

lasI-P86G/A

ABCDFigure S5

EFFigure S6

PAO1PAO1(vqsM)vqsM/p-vqsM12 hrExoTExoS

24 hrExoTExoS

AB

CDABFigure S7

1 2 3 4Figure S8

VqsMt -6His (M)+C12Protein-DNAlasI-pVqsMt -6His (M)+PQSVqsMt -6His (M)VqsMt -6His (M) +C4Protein-DNAlasI-p0.50.20.101.00.050.0250.50.20.101.00.050.0250.50.20.101.00.050.0250.50.20.101.00.050.025

0 0.5 1 2 uM 0 0.5 1 2 0.5 1 2VqsM only VqsM+LasR LasR only lasI-p Protein-DNAVqsM only ExsA only VqsM+ExsA

.05 .1 .2 .4 .05 .1 .2 .4 uM 0 .05 .1 .2 .4 lasI-p Protein-DNAVqsM only RsaL only VqsM+RsaL

.05 .1 .2 .4 .05 .1 .2 .4 uM 0 .05 .1 .2 .4 lasI-p Protein-DNAFigure S9ABC