TT

TT T T

TT

Putative DNA-binding domain

THelix Turn

Figure S1

Figure S2rhlI-p vqsR-p

0.5

1.0

0.2

0.10 0.5

1.0

0.2

0.10

VqsMt-6His (μM)

A

B C55

17

24

33

40

72100

11

KDa

1 2

Protein name Position(Start to End)

Protein name Position(Start to End)

VqsMt1 WRPLQ to RNSFH VqsMt6 FHVNV to RNSFH

VqsMt2 PFAAE to RNSFH VqsMt7 HEGND to RNSFH

VqsMt3 VIGLA to RNSFH VqsMt8 PAHQL to RRWTG

VqsMt4 IRAYV to RNSFH VqsMt9 PAVFQ to RRWTG

VqsMt5 EVEIH to RNSFH VqsMt PAHQL to RNSFH

Figure S3

10

10000

20000

30000

40000

50000

60000Ex

pres

sion

of l

asI-l

uxCP

S/O

D60

0

4 hr 12 hr 24 hr

lasI-P100T/C lasI-P99G/TlasI-P98A/G lasI-P97T/G

lasI-P96C/A

VqsMt-6His (μM)

Free DNA

Protein- DNA0.

5

1.0

0.2

0.10 0.5

1.0

0.2

0.10 0.5

1.0

0.2

0.10 0.5

1.0

0.2

0.10

Free DNA

Protein- DNA

lasI-P95T/G lasI-P94T/C

VqsMt-6His (μM)

Free DNA

Protein- DNA

lasI-P93T/G lasI-P92T/A lasI-P91C/AlasI-P90G/T lasI-P89G/A

0.5

1.0

0.2

0.10 0.5

1.0

0.2

0.10 0.5

1.0

0.2

0.10 0.5

1.0

0.2

0.10 0.5

1.0

0.2

0.10

Figure S4lasI-P88A/T

Free DNAProtein- DNA

lasI-P87C/A lasI-P86G/A

A B

C D

Figure S5

E F

Figure S6

PAO1

PAO1

(∆vq

sM)

∆vqs

M/p

-vqs

M

12 hr

ExoT

ExoS

24 hr

ExoT

ExoS

A B

C D

A

B

Figure S7

1 2 3 4

Figure S8

VqsMt -6His (μM)+C12

Protein-DNA

lasI-p

VqsMt -6His (μM)+PQS

VqsMt -6His (μM)

VqsMt -6His (μM) +C4

Protein-DNA

lasI-p

0.5

0.2

0.1

0 1.0

0.05

0.02

5

0.5

0.2

0.1

0 1.0

0.05

0.02

5

0.5

0.2

0.1

0 1.0

0.05

0.02

5 0.5

0.2

0.1

0 1.0

0.05

0.02

5

0 0.5 1 2 uM 0 0.5 1 2 0.5 1 2

VqsM only VqsM+LasR LasR only

lasI-p

Protein-DNA

VqsM only ExsA only VqsM+ExsA

.05 .1 .2 .4 .05 .1 .2 .4 uM 0 .05 .1 .2 .4

lasI-p

Protein-DNA

VqsM only RsaL only VqsM+RsaL

.05 .1 .2 .4 .05 .1 .2 .4 uM 0 .05 .1 .2 .4

lasI-p

Protein-DNA

Figure S9

A

B

C