SAN TA C RUZ BI OTEC HNOL OG Y, INC . AP-2 α (3B5 ... α,AP-2 β andAP-2 γ,...
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SANTA CRUZ BIOTECHNOLOGY, INC. AP-2α (3B5): sc-12726 Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com BACKGROUND AP-2 transcription factor family members include AP-2α, AP-2β and AP-2γ, which specifically bind to the DNA consensus sequence CCCCAGGC and ini- tiate transcription of selected genes. AP-2, also known as ERF-1, plays a role in regulating estrogen receptor expression. AP-2β, a splice variant of AP-2α, inhibits AP-2 activity. Besides subscribing to the AP-2 complex, AP-2α, AP-2β and AP-2γ proteins compose the OB2-1 transcription factor complex. OB2-1 specifically upregulates expression of the proto-oncogene c-ErbB-2, which is overexpressed in 25-30% of breast cancers. The gene encoding AP-2α maps to human chromosome 6p24.3. AP-2α may play an important role in the development of ectodermal-derived tissues. Deleterious mutations involv- ing the AP-2α gene are linked to microphthalmia, corneal clouding and other anterior eye chamber defects. The ubiquitously expressed AP-4 transcription factor specifically binds to the DNA consensus sequence 5'-CAGCTG-3'. AP-4 interacts with promoters for immunoglobulin-κ gene families and simian virus 40. AP-4 may enhance the transcription of the human Huntington’s disease gene. AP-4 is a helix-loop-helix protein that contains two distinctive leucine repeat elements. REFERENCES 1. Williams, T., et al. 1988. Cloning and expression of AP-2, a cell-type-specific transcription factor that activates inducible enhancer elements. Genes Dev. 2: 1557-1569. 2. Buettner, R., et al. 1993. An alternatively spliced mRNA from the AP-2 gene encodes a negative regulator of transcriptional activation by AP-2. Mol. Cell. Biol. 13: 4174-4185. CHROMOSOMAL LOCATION Genetic locus: TFAP2A (human) mapping to 6p24.3; Tcfap2a (mouse) mapping to 13 A3.3. SOURCE AP-2α (3B5) is a mouse monoclonal antibody immunogen corresponding to the N-terminus of AP-2α of human origin. PRODUCT Each vial contains 200 μg IgG 2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. Also available as TransCruz reagent for Gel Supershift and ChIP applications, sc-12726 X, 200 μg/0.1 ml. AP-2α (3B5) is available conjugated to agarose (sc-12726 AC), 500 μg/0.25 ml agarose in 1 ml, for IP; to HRP (sc-12726 HRP), 200 μg/ml, for WB, IHC(P) and ELISA; to either phycoerythrin (sc-12726 PE), fluorescein (sc-12726 FITC), Alexa Fluor ® 488 (sc-12726 AF488), Alexa Fluor ® 546 (sc-12726 AF546), Alexa Fluor ® 594 (sc-12726 AF594) or Alexa Fluor ® 647 (sc-12726 AF647), 200 μg/ml, for WB (RGB), IF, IHC(P) and FCM; and to either Alexa Fluor ® 680 (sc-12726 AF680) or Alexa Fluor ® 790 (sc-12726 AF790), 200 μg/ml, for Near- Infrared (NIR) WB, IF and FCM. STORAGE Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. APPLICATIONS AP-2α (3B5) is recommended for detection of AP-2α of mouse, rat and human origin by Western Blotting (starting dilution 1:200, dilution range 1:100-1:1000), immunoprecipitation [1-2 μg per 100-500 μg of total protein (1 ml of cell lysate)], immunofluorescence (starting dilution 1:50, dilution range 1:50-1:500) and immunohistochemistry (including paraffin-embedded sections) (starting dilution 1:50, dilution range 1:50-1:500). Suitable for use as control antibody for AP-2α siRNA (h): sc-105074, AP-2α siRNA (m): sc-29697, AP-2α shRNA Plasmid (h): sc-105074-SH, AP-2α shRNA Plasmid (m): sc-29697-SH, AP-2α shRNA (h) Lentiviral Particles: sc-105074-V and AP-2α shRNA (m) Lentiviral Particles: sc-29697-V. AP-2α (3B5) X TransCruz antibody is recommended for Gel Supershift and ChIP applications. Molecular Weight of AP-2α: 48 kDa. Positive Controls: AP-2α (h): 293T Lysate: sc-113722, HeLa whole cell lysate: sc-2200 or HeLa + PMA nuclear extract: sc-2121. DATA SELECT PRODUCT CITATIONS 1. Piechocki, M.P., et al. 2003. Human ErbB-2 (Her-2) transgenic mice: a model system for testing Her-2 based vaccines. J. Immunol. 171: 5787-5794. 2. Sun, X.M., et al. 2003. The transmembrane domain and PXXP motifs of ApoE receptor 2 exclude it from carrying out clathrin-mediated endocytosis. J. Biol. Chem. 278: 19926-19932. 3. Carey, T.S., et al. 2015. BRG1 Governs nanog transcription in early mouse embryos and embryonic stem cells via antagonism of Histone H3 lysine 9/14 acetylation. Mol. Cell. Biol. 35: 4158-4169. 4. Guye, P., et al. 2016. Genetically engineering self-organization of human pluripotent stem cells into a liver bud-like tissue using Gata6. Nat. Commun. 7: 10243. RESEARCH USE For research use only, not for use in diagnostic procedures. Alexa Fluor ® is a trademark of Molecular Probes, Inc., Oregon, USA AP-2α (3B5): sc-12726. Immunofluorescence stain- ing of methanol-fixed HeLa cells showing nuclear staining (A). Immunoperoxidase staining of formalin fixed, paraffin-embedded human placenta tissue showing nuclear staining of trophoblastic cells (B). B A AP-2α (3B5): sc-12726. Western blot analysis of AP-2α expression in non-transfected 293T: sc-117752 (A), human AP-2α transfected 293T: sc-113722 (B) and HeLa (C) whole cell lysates. 90K– 55K– 43K– 34K– A B C < AP-2α
Transcript of SAN TA C RUZ BI OTEC HNOL OG Y, INC . AP-2 α (3B5 ... α,AP-2 β andAP-2 γ,...
SANTA CRUZ BIOTECHNOLOGY, INC.
AP-2α (3B5): sc-12726
Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com
BACKGROUND
AP-2 transcription factor family members include AP-2α, AP-2β and AP-2γ,which specifically bind to the DNA consensus sequence CCCCAGGC and ini-tiate transcription of selected genes. AP-2, also known as ERF-1, plays a rolein regulating estrogen receptor expression. AP-2β, a splice variant of AP-2α,inhibits AP-2 activity. Besides subscribing to the AP-2 complex, AP-2α, AP-2βand AP-2γ proteins compose the OB2-1 transcription factor complex. OB2-1specifically upregulates expression of the proto-oncogene c-ErbB-2, whichis overexpressed in 25-30% of breast cancers. The gene encoding AP-2αmaps to human chromosome 6p24.3. AP-2α may play an important role inthe development of ectodermal-derived tissues. Deleterious mutations involv-ing the AP-2α gene are linked to microphthalmia, corneal clouding and otheranterior eye chamber defects. The ubiquitously expressed AP-4 transcriptionfactor specifically binds to the DNA consensus sequence 5'-CAGCTG-3'. AP-4interacts with promoters for immunoglobulin-κ gene families and simianvirus 40. AP-4 may enhance the transcription of the human Huntington’sdisease gene. AP-4 is a helix-loop-helix protein that contains two distinctiveleucine repeat elements.
REFERENCES
1. Williams, T., et al. 1988. Cloning and expression of AP-2, a cell-type-specifictranscription factor that activates inducible enhancer elements. GenesDev. 2: 1557-1569.
2. Buettner, R., et al. 1993. An alternatively spliced mRNA from the AP-2gene encodes a negative regulator of transcriptional activation by AP-2.Mol. Cell. Biol. 13: 4174-4185.
CHROMOSOMAL LOCATION
Genetic locus: TFAP2A (human) mapping to 6p24.3; Tcfap2a (mouse) mappingto 13 A3.3.
SOURCE
AP-2α (3B5) is a mouse monoclonal antibody immunogen corresponding tothe N-terminus of AP-2α of human origin.
PRODUCT
Each vial contains 200 µg IgG2b kappa light chain in 1.0 ml of PBS with < 0.1%sodium azide and 0.1% gelatin. Also available as TransCruz reagent for GelSupershift and ChIP applications, sc-12726 X, 200 µg/0.1 ml.
AP-2α (3B5) is available conjugated to agarose (sc-12726 AC), 500 µg/0.25 mlagarose in 1 ml, for IP; to HRP (sc-12726 HRP), 200 µg/ml, for WB, IHC(P)and ELISA; to either phycoerythrin (sc-12726 PE), fluorescein (sc-12726 FITC),Alexa Fluor® 488 (sc-12726 AF488), Alexa Fluor® 546 (sc-12726 AF546),Alexa Fluor® 594 (sc-12726 AF594) or Alexa Fluor® 647 (sc-12726 AF647),200 µg/ml, for WB (RGB), IF, IHC(P) and FCM; and to either Alexa Fluor® 680(sc-12726 AF680) or Alexa Fluor® 790 (sc-12726 AF790), 200 µg/ml, for Near-Infrared (NIR) WB, IF and FCM.
STORAGE
Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date ofshipment. Non-hazardous. No MSDS required.
APPLICATIONS
AP-2α (3B5) is recommended for detection of AP-2α of mouse, rat andhuman origin by Western Blotting (starting dilution 1:200, dilution range1:100-1:1000), immunoprecipitation [1-2 µg per 100-500 µg of total protein(1 ml of cell lysate)], immunofluorescence (starting dilution 1:50, dilutionrange 1:50-1:500) and immunohistochemistry (including paraffin-embeddedsections) (starting dilution 1:50, dilution range 1:50-1:500).
Suitable for use as control antibody for AP-2α siRNA (h): sc-105074, AP-2αsiRNA (m): sc-29697, AP-2α shRNA Plasmid (h): sc-105074-SH, AP-2α shRNAPlasmid (m): sc-29697-SH, AP-2α shRNA (h) Lentiviral Particles: sc-105074-Vand AP-2α shRNA (m) Lentiviral Particles: sc-29697-V.
AP-2α (3B5) X TransCruz antibody is recommended for Gel Supershift andChIP applications.
Molecular Weight of AP-2α: 48 kDa.
Positive Controls: AP-2α (h): 293T Lysate: sc-113722, HeLa whole cell lysate:sc-2200 or HeLa + PMA nuclear extract: sc-2121.
DATA
SELECT PRODUCT CITATIONS
1. Piechocki, M.P., et al. 2003. Human ErbB-2 (Her-2) transgenic mice: a modelsystem for testing Her-2 based vaccines. J. Immunol. 171: 5787-5794.
2. Sun, X.M., et al. 2003. The transmembrane domain and PXXP motifs ofApoE receptor 2 exclude it from carrying out clathrin-mediated endocytosis.J. Biol. Chem. 278: 19926-19932.
3. Carey, T.S., et al. 2015. BRG1 Governs nanog transcription in early mouseembryos and embryonic stem cells via antagonism of Histone H3 lysine9/14 acetylation. Mol. Cell. Biol. 35: 4158-4169.
4. Guye, P., et al. 2016. Genetically engineering self-organization of humanpluripotent stem cells into a liver bud-like tissue using Gata6. Nat.Commun. 7: 10243.
RESEARCH USE
For research use only, not for use in diagnostic procedures.
Alexa Fluor® is a trademark of Molecular Probes, Inc., Oregon, USA
AP-2α (3B5): sc-12726. Immunofluorescence stain-ing of methanol-fixed HeLa cells showing nuclearstaining (A). Immunoperoxidase staining of formalinfixed, paraffin-embedded human placenta tissueshowing nuclear staining of trophoblastic cells (B).
BA
AP-2α (3B5): sc-12726. Western blot analysis of AP-2αexpression in non-transfected 293T: sc-117752 (A),human AP-2α transfected 293T: sc-113722 (B) andHeLa (C) whole cell lysates.
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34 K –
A B C
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