For Research Use Only FASN Polyclonal ANTIBODY · Detection:10624-2-AP 1:500) with mouse liver...
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FASN Polyclonal antibody Catalog Number: 10624-2-AP Featured Product 57 Publications For Research Use Only www.ptglab.com Basic Information Catalog Number: 10624-2-AP Size: 150UL , Concentration: 360 μg/ml by Bradford method using BSA as the standard; Source: Rabbit Isotype: IgG Immunogen Catalog Number: AG0975 GenBank Accession Number: BC007909 GeneID (NCBI): 2194 Full Name: fatty acid synthase Calculated MW: 272 kDa Observed MW: 250-272 kDa Purification Method: Antigen affinity purification Recommended Dilutions: WB 1:200-1:1000 IP 0.5-4.0 ug for IP and 1:500-1:1000 for WB IHC 1:50-1:500 IF 1:10-1:100 Applications Tested Applications: FC, IF, IHC, IP, WB, ELISA Cited Applications: IHC, WB Species Specificity: human, mouse, rat Cited Species: chicken, human, mouse, rat, swine Note-IHC: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 Positive Controls: WB : rat liver tissue, L02 cells, HepG2 cells, HT-29 cells, HeLa cells, mouse liver tissue IP : mouse liver tissue, IHC : human colon cancer tissue, IF : Hela cells, HepG2 cells Background Information FASN gene codes for an enzyme essential for de novo fatty acid synthesis and cellular substrate energy metabolism. Active FASN is a homodimer in which each peptide subunit has a molecular weight of 260 kDa. FASN is overexpressed in various types of cancer including glioblastomas and is a potential therapeutic target. Recently FASN has been reported to contribute to the neurogenesis since FASN mutation caused intellectual disability in mice. Notable Publications Author Pubmed ID Journal Application Jia-Shun Wu 30233549 Front Microbiol IHC Yuezhu Zhang 31491605 Ecotoxicol Environ Saf WB Siyu Chen 29084766 FASEB J WB Storage Storage: Store at -20°C. Stable for one year after shipment. Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Aliquoting is unnecessary for -20 º C storage T: 1 (888) 4PTGLAB (1-888-478-4522) (toll free in USA), or 1(312) 455-8498 (outside USA) E: [email protected] W: ptglab.com For technical support and original validation data for this product please contact: This product is exclusively available under Proteintech Group brand and is not available to purchase from any other manufacturer.
Transcript of For Research Use Only FASN Polyclonal ANTIBODY · Detection:10624-2-AP 1:500) with mouse liver...
FASN Polyclonal antibodyCatalog Number:10624-2-AP Featured Product 57 Publications
For Research Use Only
www.ptglab.com
Basic Information Catalog Number:10624-2-AP
Size:150UL , Concentration: 360 μg/ml byBradford method using BSA as thestandard;
Source:Rabbit
Isotype:IgG
Immunogen Catalog Number:AG0975
GenBank Accession Number:BC007909
GeneID (NCBI):2194
Full Name:fatty acid synthase
Calculated MW:272 kDa
Observed MW:250-272 kDa
Purification Method:Antigen affinity purification
Recommended Dilutions:WB 1:200-1:1000 IP 0.5-4.0 ug for IP and 1:500-1:1000for WBIHC 1:50-1:500 IF 1:10-1:100
Applications Tested Applications:FC, IF, IHC, IP, WB, ELISA
Cited Applications:IHC, WB
Species Specificity:human, mouse, rat
Cited Species:chicken, human, mouse, rat, swine
Note-IHC: suggested antigen retrieval withTE buffer pH 9.0; (*) Alternatively, antigenretrieval may be performed with citratebuffer pH 6.0
Positive Controls:
WB : rat liver tissue, L02 cells, HepG2 cells, HT-29 cells,HeLa cells, mouse liver tissue
IP : mouse liver tissue,
IHC : human colon cancer tissue,
IF : Hela cells, HepG2 cells
Background Information FASN gene codes for an enzyme essential for de novo fatty acid synthesis and cellular substrate energy metabolism.Active FASN is a homodimer in which each peptide subunit has a molecular weight of 260 kDa. FASN isoverexpressed in various types of cancer including glioblastomas and is a potential therapeutic target. RecentlyFASN has been reported to contribute to the neurogenesis since FASN mutation caused intellectual disability inmice.
Notable Publications Author Pubmed ID Journal Application
Jia-Shun Wu 30233549 Front Microbiol IHC
Yuezhu Zhang 31491605 Ecotoxicol Environ Saf WB
Siyu Chen 29084766 FASEB J WB
Storage Storage:Store at -20°C. Stable for one year after shipment.Storage Buffer:PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Aliquoting is unnecessary for -20ºC storage
T: 1 (888) 4PTGLAB (1-888-478-4522) (toll freein USA), or 1(312) 455-8498 (outside USA)
E: [email protected] W: ptglab.com
For technical support and original validation data for this product please contact: This product is exclusively available under ProteintechGroup brand and is not available to purchase from anyother manufacturer.
Selected Validation Data
Various lysates were subjected to SDS PAGEfollowed by western blot with 10624-2-AP (FASNantibody) at dilution of 1:2000 incubated at roomtemperature for 1.5 hours.
rat liver tissue were subjected to SDS PAGEfollowed by western blot with 10624-2-AP (FASNAntibody) at dilution of 1:300 incubated at roomtemperature for 1.5 hours.
Immunofluorescent analysis of Hela cells, usingFASN antibody 10624-2-AP at 1:25 dilution andRhodamine-labeled goat anti-rabbit IgG (red). Bluepseudocolor = DAPI (fluorescent DNA dye).
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using10624-2-AP (FASN antibody) at dilution of 1:200(under 10x lens). Heat mediated antigen retrievalwith Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using10624-2-AP (FASN antibody) at dilution of 1:200(under 10x lens). Heat mediated antigen retrievalwith Tris-EDTA buffer (pH 9.0).
IP Result of anti-FASN (IP:10624-2-AP, 4ug;Detection:10624-2-AP 1:500) with mouse livertissue lysate 4000ug.
1X10^6 HeLa cells were stained with 0.2ug FASNantibody (10624-2-AP, red) and control antibody(blue). Fixed with 90% MeOH blocked with 3% BSA(30 min). Alexa Fluor 488-conjugated AffiniPureGoat Anti-Rabbit IgG(H+L) with dilution 1:1000.
