Polyclonal ANTIBODY ST7 - Proteintech · 2018. 12. 22. · Selected Validation Data mouse brain...
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ST7 Polyclonal ANTIBODY Catalog Number: 11945-1-AP 1 Publications For Research Use Only www.ptglab.com Basic Information Catalog Number: 11945-1-AP Size: 150UL , Concentration: 207 μg/ml by Bradford method using BSA as the standard; Source: Rabbit Isotype: IgG Immunogen Catalog Number: AG2547 GenBank Accession Number: BC030954 GeneID (NCBI): 7982 Full Name: suppression of tumorigenicity 7 Calculated MW: 63 kDa Observed MW: 63 kDa Purification Method: Antigen affinity purification Recommended Dilutions: WB 1:1000-1:4000 IP 0.5-4.0 ug for IP and 1:500-1:3000 for WB IHC 1:20-1:200 Applications Tested Applications: IHC, IP, WB, ELISA Cited Applications: WB Species Specificity: human, mouse Cited Species: human Note-IHC: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 Positive Controls: WB : mouse brain tissue, HeLa cells, human brain tissue, Jurkat cells, K-562 cells, PC-3 cells IP : PC-3 cells, IHC : human liver tissue, human heart tissue, human lymphoma tissue, human pancreas tissue Background Information Human ST7 (suppressor of tumorigenicity 7) gene, also called FAM4A1, HELG or RAY1, maps to a region on chromosome 7 identified as an autism-susceptibility locus. ST7 has been proposed to be a tumor suppressor gene with highest expression in heart, liver and pancreas. The product of this gene is a multi-pass membrane protein belonging to the ST7 family, full-length ST7 cDNA has been cloned and verified in cancer cell lines, recent result suggests that ST7 may mediate tumor suppression through the regulation of the genes involved in maintaining the cellular structure of the cell and involved in oncogenic pathways. Notable Publications Author Pubmed ID Journal Application Fengting Yan 24453002 Cancer Res WB Storage Storage: Store at -20°C. Stable for one year after shipment. Storage Buffer: PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Aliquoting is unnecessary for -20 º C storage T: 1 (888) 4PTGLAB (1-888-478-4522) (toll free in USA), or 1(312) 455-8498 (outside USA) E: [email protected] W: ptglab.com For technical support and original validation data for this product please contact: This product is exclusively available under Proteintech Group brand and is not available to purchase from any other manufacturer.
Transcript of Polyclonal ANTIBODY ST7 - Proteintech · 2018. 12. 22. · Selected Validation Data mouse brain...
ST7 Polyclonal ANTIBODYCatalog Number:11945-1-AP 1 Publications
For Research Use Only
www.ptglab.com
Basic Information Catalog Number:11945-1-AP
Size:150UL , Concentration: 207 μg/ml byBradford method using BSA as thestandard;
Source:Rabbit
Isotype:IgG
Immunogen Catalog Number:AG2547
GenBank Accession Number:BC030954
GeneID (NCBI):7982
Full Name:suppression of tumorigenicity 7
Calculated MW:63 kDa
Observed MW:63 kDa
Purification Method:Antigen affinity purification
Recommended Dilutions:WB 1:1000-1:4000 IP 0.5-4.0 ug for IP and 1:500-1:3000for WBIHC 1:20-1:200
Applications Tested Applications:IHC, IP, WB, ELISA
Cited Applications:WB
Species Specificity:human, mouse
Cited Species:human
Note-IHC: suggested antigen retrieval withTE buffer pH 9.0; (*) Alternatively, antigenretrieval may be performed with citratebuffer pH 6.0
Positive Controls:
WB : mouse brain tissue, HeLa cells, human braintissue, Jurkat cells, K-562 cells, PC-3 cells
IP : PC-3 cells,
IHC : human liver tissue, human heart tissue, humanlymphoma tissue, human pancreas tissue
Background Information Human ST7 (suppressor of tumorigenicity 7) gene, also called FAM4A1, HELG or RAY1, maps to a region onchromosome 7 identified as an autism-susceptibility locus. ST7 has been proposed to be a tumor suppressor genewith highest expression in heart, liver and pancreas. The product of this gene is a multi-pass membrane proteinbelonging to the ST7 family, full-length ST7 cDNA has been cloned and verified in cancer cell lines, recent resultsuggests that ST7 may mediate tumor suppression through the regulation of the genes involved in maintaining thecellular structure of the cell and involved in oncogenic pathways.
Notable Publications Author Pubmed ID Journal Application
Fengting Yan 24453002 Cancer Res WB
Storage Storage:Store at -20°C. Stable for one year after shipment.Storage Buffer:PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Aliquoting is unnecessary for -20ºC storage
T: 1 (888) 4PTGLAB (1-888-478-4522) (toll freein USA), or 1(312) 455-8498 (outside USA)
E: [email protected] W: ptglab.com
For technical support and original validation data for this product please contact: This product is exclusively available under ProteintechGroup brand and is not available to purchase from anyother manufacturer.
Selected Validation Data
mouse brain tissue were subjected to SDS PAGEfollowed by western blot with 11945-1-AP (ST7Antibody) at dilution of 1:2000 incubated at roomtemperature for 1.5 hours.
IP Result of anti-ST7 (IP:11945-1-AP, 4ug;Detection:11945-1-AP 1:1500) with PC-3 cellslysate 2640ug.
Immunohistochemical analysis of paraffin-embedded human liver using 11945-1-AP (ST7antibody) at dilution of 1:50 (under 10x lens).
Immunohistochemical analysis of paraffin-embedded human liver using 11945-1-AP (ST7antibody) at dilution of 1:50 (under 40x lens).
