HSP90Polyclonal ANTIBODYCatalog Number:13171-1-AP Featured Product 38 Publications

For Research Use Only

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Basic Information Catalog Number:13171-1-APSize:44 μg/150 μlSource:RabbitIsotype:IgGPurif ication Method:Antigen aff inity purif icationImmunogen Catalog Number:AG3826

GenBank Accession Number:BC023006GeneID (NCBI):3320Full Name:heat shock protein 90kDa alpha (cytosolic),class A member 1Calculated MW:853aa,90 kDaObserved MW:90 kDa

Recommended Dilutions:WB 1:1000-1:6000 IP 0.5-4.0 ug for IP and 1:500-1:1000 for WBIHC 1:20-1:200 IF 1:10-1:100

Applications Tested Applications:FC, IF, IHC, IP, WB, ELISACited Applications:CoIP, ELISA, IF, IHC, WBSpecies Specif icity:human,mouse,ratCited Species:chicken, human, mouse, ratNote: suggested an gen retrieval with TEbuffer pH 9.0; (*) Alterna vely, an genretrieval may be performed with citratebuffer pH 6.0

Positive Controls:WB : HeLa cells; 3T3-L1 cells, C6 cells, HEK-293cells, human liver tissue, K-562 cells, mouse kidneytissue, rat brain tissue, Y79 cellsIP : K-562 cells; HeLa cellsIHC : human liver cancer tissue;IF : HepG2 cells;

Background Information HSP90, encoded by HSP90AA1, is a constitutively and ubiquitously expressed molecular chaperone that is crucial for the stability and function of manyproteins. HSP90 provides chaperoning activity for client proteins; many of them are members of oncogenic pathways, indicating its implication in tumormalignancy. HSP90 mainly resides in the cytosol, while it can also be released to the extracellular space. Secreted Hsp90 is a C-terminal truncated form. Ithas been reported that the level of plasma Hsp90 is positively correlated with tumor malignancy in clinical cancer patients, and can be a promising diagnosticmarker for tumor malignancy in clinical application.

Notable Publications Author Pubmed ID Journal Application

Bing Sun 27684953 PLoS One WB

Jeremy T Lant 28933646 RNA Biol WB

Shuchao Wang 30240910 Ann Anat WB

Storage Storage:Store at -20ºC. Stable for one year af ter shipment.Storage Buffer:PBS with 0.02% sodium azide and 50% glycerol pH 7.3.Aliquoting is unnecessary for -20ºC storage

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Selected Validation Data

Various lysates were subjected to SDS PAGEfollowed by western blot with 13171-1-AP (HSP90antibody) at dilution of 1:8000 incubated at roomtemperature for 1.5 hours

HeLa cells were subjected to SDS PAGE followedby western blot with 13171-1-AP( HSP90Antibody) at dilution of 1:3000 incubated at 4degree celsius over night

IP Result of anti-HSP90 (IP:13171-1-AP, 3ug;Detection:13171-1-AP 1:500) with K-562 cellslysate 2000ug.

Immunohistochemical of paraff in-embeddedhuman liver cancer using 13171-1-AP(HSP90antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemical of paraff in-embeddedhuman liver cancer using 13171-1-AP(HSP90antibody) at dilution of 1:50 (under 40x lens)

1X10̂ 6 HeLa cells were stained with 0.2ug HSP90antibody (13171-1-AP, red) and control antibody(blue). Fixed with 90% MeOH blocked with 3%BSA (30 min). Alexa Fluor 488-conjugatedAff iniPure Goat Anti-Rabbit IgG(H+L) with dilution1:1500.

Immunofluorescent analysis of HepG2 cells,using HSP90AA1 antibody 13171-1-AP at 1:25dilution and Rhodamine-labeled goat anti-rabbitIgG (red).