Immunofluorescence Antibody Customer Review for Laminin α-1 Polyclonal Antibody (STJ93888)

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HOW DID OUR ANTIBODY PERFORM? ANTIBODY CUSTOMER REVIEW: Laminin a-1 Polyclonal Antibody (STJ93888)

Transcript of Immunofluorescence Antibody Customer Review for Laminin α-1 Polyclonal Antibody (STJ93888)

Page 1: Immunofluorescence Antibody Customer Review for Laminin α-1 Polyclonal Antibody (STJ93888)

HOW DID OUR ANTIBODY PERFORM?

ANTIBODY CUSTOMER REVIEW: Laminin a-1 Polyclonal Antibody (STJ93888)

Page 2: Immunofluorescence Antibody Customer Review for Laminin α-1 Polyclonal Antibody (STJ93888)

What was used?Primary antibody: STJ93888 Laminin a-1

Provider: St John's Laboratory Ltd.

Dilution ratio: 1:50

Application: Immunofluorescence staining

Materials for validation: Decellularised cornea

Click for product data sheet PDF

Page 3: Immunofluorescence Antibody Customer Review for Laminin α-1 Polyclonal Antibody (STJ93888)

What Was The Protocol?Treatment of Materials Tissue was sectioned at a 7 um thickness, and then fixed with 4% parafolmaldehyde and

permeabilized with 1% Triton X-100.

Fixation 4 % parafolmadehyde at room temperature for 20 minutes.

Permeabilization 1% Triton X-100 at room temperature for 5 minutes.

Blocking Blocking buffer (PBS with BSA and goat serum) for 1 hour.

Primary Antibody Probing Primary antibody was added to the solution of blocking buffer (PBS with BSA and goat serum), at 4˚C overnight.

Sample Wash The sample was washed with PBS with BSA and goat serum 5 times, each for 5 minutes.

Secondary Antibody Probing Secondary antibody was added to the solution of blocking buffer (PBS with BSA and goat serum) with a 1:100 dilution ratio, for 1 hour.

Sample Wash The sample was washed with PBS with BSA and goat serum 7 times, each for 5 minutes.

Visualization Nikon Axiovert microscope.

Page 4: Immunofluorescence Antibody Customer Review for Laminin α-1 Polyclonal Antibody (STJ93888)

What was the protocol?

No. Antigen Primary antibody

Primary antibody

dilution ratio

Secondaryantibody

dilution ratio

1 Decellularised cornea

STJ93888 Laminin a-1

antibody1:50 1:00

Page 5: Immunofluorescence Antibody Customer Review for Laminin α-1 Polyclonal Antibody (STJ93888)

What were the results?

Page 6: Immunofluorescence Antibody Customer Review for Laminin α-1 Polyclonal Antibody (STJ93888)

What did the customer think?

Antibody Specificity:

Antibody RPPA Rating:

Testing results were provided independently by F. Abidin at Newcastle University. They stated “Visible laminin staining was observed from this tissue section.”

Page 8: Immunofluorescence Antibody Customer Review for Laminin α-1 Polyclonal Antibody (STJ93888)

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Page 9: Immunofluorescence Antibody Customer Review for Laminin α-1 Polyclonal Antibody (STJ93888)

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