In Cell-Western Antibody Customer Review for Anti-MAP LC3β antibody (STJ97398)
1
Result “I have used them in LI-COR IN CELL WESTERN method. They worked nicely. The method was according to the in- strucons provided with the LI-COR kit with their buffers and anbodies. ” - S. Morshed, Icahn School of Medicine at Mount Sinai and Bronx. Gel electrophoresis informaon：N/A Transfer informaon：N/A Lane No. Angen Loading amount Primary anbody Primary anbody diluon rao Secondary anbody diluon rao Exposure me Control-Ab Thyrocytes 50,000 cells/well STJ97398 An- MAP LC3β 1:100 1:800 10Min Cell Line: Rat Thyrocytes Method of validaon: LI-COR CELL Western Primary Anbody: STJ97398 Anti-MAP LC3β anti- body Secondary Anbody LI-COR Anbody Diluon rao: 1:100 Protocol Treatment of materials: Rat thyrocytes were treated with TSH receptor anbody for 24 hrs. Cell fixing: 3.7% formaldehyde in 1xPBS, for 20 minutes at room temperature. Washing: 1XPBS with 0.1% Triton X-100 for 5 minutes per wash. Repeat 4 mes. Blocking: LI-COR Blocking buffer, 1.5 hours at room temperature with moderate shaking. Primary anbody probing: Overnight at 4°C. Washing: 1XPBS with 0.1% Tween 20 for 5 minutes at room temperature. Secondary anbody probing: LI-COR Anbody for 1 hour. Washing: 1XPBS with 0.1% Tween 20 for 5 minutes at room temperature. Repeat 5 mes. Visualizaon: Analysed by LI-COR imaging 10 minutes. Anbody Customer Review: STJ97398 An-MAP LC3β Anbody Anbody Specificity: Anbody Rang: Fig.1 - Rat thyrocytes were treated with TSH re- ceptor anbody for 24 hrs. Fixed, permeabilized and then stained with primary anbodies. Aſter washing FAR red conjugated secondary anbody was used and then analyzed by LI-COR imaging.
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Transcript of In Cell-Western Antibody Customer Review for Anti-MAP LC3β antibody (STJ97398)
Result
“I have used them in LI-COR IN CELL WESTERN method. They worked nicely. The method was according to the in-structions provided with the LI-COR kit with their buffers and antibodies.” - S. Morshed, Icahn School of Medicine at Mount Sinai and Bronx.
Gel electrophoresis information：N/A
Transfer information：N/A
Lane No. Antigen Loading amount Primary antibody Primary antibody dilution ratio
Secondary
antibody dilution ratio
Exposure time
Control-Ab Thyrocytes 50,000 cells/well STJ97398 Anti-
MAP LC3β 1:100 1:800 10Min
Cell Line: Rat Thyrocytes
Method of validation: LI-COR CELL Western
Primary Antibody: STJ97398 Anti-MAP LC3β anti-
body
Secondary Antibody LI-COR Antibody
Dilution ratio: 1:100
Protocol
Treatment of materials: Rat thyrocytes were treated with TSH receptor antibody for 24 hrs.
Cell fixing: 3.7% formaldehyde in 1xPBS, for 20 minutes at room temperature.
Washing: 1XPBS with 0.1% Triton X-100 for 5 minutes per wash. Repeat 4 times.
Blocking: LI-COR Blocking buffer, 1.5 hours at room temperature with moderate shaking.
Primary antibody probing: Overnight at 4°C.
Washing: 1XPBS with 0.1% Tween 20 for 5 minutes at room temperature.
Secondary antibody probing: LI-COR Antibody for 1 hour.
Washing: 1XPBS with 0.1% Tween 20 for 5 minutes at room temperature. Repeat 5 times.
Visualization: Analysed by LI-COR imaging 10 minutes.
Antibody Customer Review: STJ97398 Anti-MAP LC3β Antibody Antibody Specificity:
Antibody Rating:
Fig.1 - Rat thyrocytes were treated with TSH re-ceptor antibody for 24 hrs. Fixed, permeabilized and then stained with primary antibodies. After washing FAR red conjugated secondary antibody was used and then analyzed by LI-COR imaging.
