For Research Use Only ACSF2 Polyclonal antibody · 2021. 4. 12. · ACSF2 Polyclonal antibody...
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ACSF2 Polyclonal antibody Catalog Number: 16140-1-AP For Research Use Only www.ptglab.com Basic Information Catalog Number: 16140-1-AP Size: 150UL , Concentration: 300 μg/ml by Bradford method using BSA as the standard; Source: Rabbit Isotype: IgG Immunogen Catalog Number: AG9199 GenBank Accession Number: BC012053 GeneID (NCBI): 80221 Full Name: acyl-CoA synthetase family member 2 Calculated MW: 615 aa, 68 kDa Observed MW: 55-70 kDa Purification Method: Antigen affinity purification Recommended Dilutions: WB 1:500-1:1000 IHC 1:20-1:200 IF 1:20-1:200 Applications Tested Applications: IF, IHC, WB,ELISA Species Specificity: human, mouse, rat Note-IHC: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 Positive Controls: WB : mouse lung tissue, IHC : human skin tissue, IF : A549 cells, Background Information Storage Storage: Store at -20°C. Stable for one year after shipment. Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Aliquoting is unnecessary for -20 º C storage T: 1 (888) 4PTGLAB (1-888-478-4522) (toll free in USA), or 1(312) 455-8498 (outside USA) E: [email protected] W: ptglab.com For technical support and original validation data for this product please contact: This product is exclusively available under Proteintech Group brand and is not available to purchase from any other manufacturer.
Transcript of For Research Use Only ACSF2 Polyclonal antibody · 2021. 4. 12. · ACSF2 Polyclonal antibody...
ACSF2 Polyclonal antibodyCatalog Number:16140-1-AP
For Research Use Only
www.ptglab.com
Basic Information Catalog Number:16140-1-AP
Size:150UL , Concentration: 300 μg/ml byBradford method using BSA as thestandard;
Source:Rabbit
Isotype:IgG
Immunogen Catalog Number:AG9199
GenBank Accession Number:BC012053
GeneID (NCBI):80221
Full Name:acyl-CoA synthetase family member2
Calculated MW:615 aa, 68 kDa
Observed MW:55-70 kDa
Purification Method:Antigen affinity purification
Recommended Dilutions:WB 1:500-1:1000 IHC 1:20-1:200 IF 1:20-1:200
Applications Tested Applications:IF, IHC, WB,ELISA
Species Specificity:human, mouse, rat
Note-IHC: suggested antigen retrieval withTE buffer pH 9.0; (*) Alternatively, antigenretrieval may be performed with citratebuffer pH 6.0
Positive Controls:
WB : mouse lung tissue,
IHC : human skin tissue,
IF : A549 cells,
Background Information
Storage Storage:Store at -20°C. Stable for one year after shipment.Storage Buffer:PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Aliquoting is unnecessary for -20ºC storage
T: 1 (888) 4PTGLAB (1-888-478-4522) (toll freein USA), or 1(312) 455-8498 (outside USA)
E: [email protected] W: ptglab.com
For technical support and original validation data for this product please contact: This product is exclusively available under ProteintechGroup brand and is not available to purchase from anyother manufacturer.
Selected Validation Data
mouse lung tissue were subjected to SDS PAGEfollowed by western blot with 16140-1-AP (ACSF2Antibody) at dilution of 1:600 incubated at 4 degreecelsius over night.
Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 16140-1-AP (ACSF2 Antibody) at dilution of 1:50 (under 10xlens).
Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 16140-1-AP (ACSF2 Antibody) at dilution of 1:50 (under 40xlens).
Immunofluorescent analysis of (4% PFA) fixedA549 cells using 16140-1-AP (ACSF2 antibody) atdilution of 1:50 and Alexa Fluor 488-conjugatedAffiniPure Goat Anti-Rabbit IgG(H+L).
