Polyclonal ANTIBODY SEPT9 · PAGE followed by western blot with 10769-1-AP(SEPT9 antibody) at...

2
SEPT9 Polyclonal ANTIBODY Catalog Number: 10769-1-AP Featured Product 12 Publications For Research Use Only www.ptglab.com Basic Information Catalog Number: 10769-1-AP Size: 150UL , Concentration: 240 μg/ml by Bradford method using BSA as the standard; Source: Rabbit Isotype: IgG Immunogen Catalog Number: AG1218 GenBank Accession Number: BC021192 GeneID (NCBI): 10801 Full Name: septin 9 Calculated MW: 64 kDa Observed MW: 65 kDa, 38-40 kDa Purification Method: Antigen affinity purification Recommended Dilutions: WB 1:500-1:1000 IP 0.5-4.0 ug for IP and 1:500-1:2000 for WB IHC 1:50-1:500 IF 1:20-1:200 Applications Tested Applications: FC, IF, IHC, IP, WB, ELISA Cited Applications: IF, IHC, WB Species Specificity: human, mouse, rat Cited Species: human, mouse, rat Note-IHC: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 Positive Controls: WB : mouse thymus tissue, C6 cells, HeLa cells, mouse brain tissue, mouse spleen tissue IP : mouse brain tissue, IHC : human pancreas cancer tissue, human testis tissue IF : HepG2 cells, hTERT-RPE cells Background Information SEPT9 belongs to the septin family which is a group of highly conserved GTP-binding proteins in eukaryotes. SEPT9 gene methylation has been implicated as a biomarker for colorectal cancer(CRC). this antibody can identify all of the isoforms about SEPT9. Notable Publications Author Pubmed ID Journal Application Sellin Mikael E ME 21737677 Mol Biol Cell WB Julia Pfanzelter 29921601 J Cell Biol IF Katharina Neubauer 30019536 Cytoskeleton (Hoboken) IF Storage Storage: Store at -20°C. Stable for one year after shipment. Storage Buffer: PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Aliquoting is unnecessary for -20 º C storage T: 1 (888) 4PTGLAB (1-888-478-4522) (toll free in USA), or 1(312) 455-8498 (outside USA) E: [email protected] W: ptglab.com For technical support and original validation data for this product please contact: This product is exclusively available under Proteintech Group brand and is not available to purchase from any other manufacturer.

Transcript of Polyclonal ANTIBODY SEPT9 · PAGE followed by western blot with 10769-1-AP(SEPT9 antibody) at...

Page 1: Polyclonal ANTIBODY SEPT9 · PAGE followed by western blot with 10769-1-AP(SEPT9 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours Immunofluorescent analysis

SEPT9 Polyclonal ANTIBODYCatalog Number:10769-1-AP Featured Product 12 Publications

For Research Use Only

www.ptglab.com

Basic Information Catalog Number:10769-1-AP

Size:150UL , Concentration: 240 μg/ml byBradford method using BSA as thestandard;

Source:Rabbit

Isotype:IgG

Immunogen Catalog Number:AG1218

GenBank Accession Number:BC021192

GeneID (NCBI):10801

Full Name:septin 9

Calculated MW:64 kDa

Observed MW:65 kDa, 38-40 kDa

Purification Method:Antigen affinity purification

Recommended Dilutions:WB 1:500-1:1000 IP 0.5-4.0 ug for IP and 1:500-1:2000for WBIHC 1:50-1:500 IF 1:20-1:200

Applications Tested Applications:FC, IF, IHC, IP, WB, ELISA

Cited Applications:IF, IHC, WB

Species Specificity:human, mouse, rat

Cited Species:human, mouse, rat

Note-IHC: suggested antigen retrieval withTE buffer pH 9.0; (*) Alternatively, antigenretrieval may be performed with citratebuffer pH 6.0

Positive Controls:

WB : mouse thymus tissue, C6 cells, HeLa cells, mousebrain tissue, mouse spleen tissue

IP : mouse brain tissue,

IHC : human pancreas cancer tissue, human testistissue

IF : HepG2 cells, hTERT-RPE cells

Background Information SEPT9 belongs to the septin family which is a group of highly conserved GTP-binding proteins in eukaryotes. SEPT9gene methylation has been implicated as a biomarker for colorectal cancer(CRC). this antibody can identify all ofthe isoforms about SEPT9.

Notable Publications Author Pubmed ID Journal Application

Sellin Mikael E ME 21737677 Mol Biol Cell WB

Julia Pfanzelter 29921601 J Cell Biol IF

Katharina Neubauer 30019536 Cytoskeleton (Hoboken) IF

Storage Storage:Store at -20°C. Stable for one year after shipment.Storage Buffer:PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Aliquoting is unnecessary for -20ºC storage

T: 1 (888) 4PTGLAB (1-888-478-4522) (toll freein USA), or 1(312) 455-8498 (outside USA)

E: [email protected] W: ptglab.com

For technical support and original validation data for this product please contact: This product is exclusively available under ProteintechGroup brand and is not available to purchase from anyother manufacturer.

Page 2: Polyclonal ANTIBODY SEPT9 · PAGE followed by western blot with 10769-1-AP(SEPT9 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours Immunofluorescent analysis

Selected Validation Data

IF result of anti-SEPT9 (10769-1-AP, 1:200) withhTERT-RPE cell by Moshe Kim (laboratory of Dr.William S Trimble, University of Toronto). Green:SEPT9. Blue: DNA. Fixation in methanol.

mouse thymus tissue were subjected to SDS PAGEfollowed by western blot with 10769-1-AP (SEPT9antibody) at dilution of 1:600 incubated at roomtemperature for 1.5 hours.

Immunofluorescent analysis of HepG2 cells, usingSEPT9 antibody 10769-1-AP at 1:50 dilution andRhodamine-labeled goat anti-rabbit IgG (red). Bluepseudocolor = DAPI (fluorescent DNA dye).

Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slideusing 10769-1-AP (SEPT9 antibody) at dilution of1:200 (under 10x lens. Heat mediated antigenretrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slideusing 10769-1-AP (SEPT9 antibody) at dilution of1:200 (under 40x lens. Heat mediated antigenretrieval with Tris-EDTA buffer (pH 9.0).

IP Result of anti-SEPT9 (IP:10769-1-AP, 4ug;Detection:10769-1-AP 1:1000) with mouse braintissue lysate 4000ug.

1X10^6 HepG2 cells were stained with 0.2ug SEPT9antibody (10769-1-AP, red) and control antibody(blue). Fixed with 90% MeOH blocked with 3% BSA(30 min). Alexa Fluor 488-conjugated AffiniPureGoat Anti-Rabbit IgG(H+L) with dilution 1:1000.