Platelet Rich Plasma Art & science Islam Kassem,BDS,MSc,FDS RCS Consultant oral & maxillofacial Surgeon ikassem@dr.com

Angle Face Left New technology of injection your own blood to get a Youthful Look . . ikassem@dr.com

Why Are Celebrities Injecting Their Faces With Blood? ikassem@dr.com

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Platelet? Anuclear piece of cytoplasm Include hemostasis, -granule,lysosomal granule, dense granules ikassem@dr.com

At least 7 different growth factors that stimulate bone & soft tissue healing PDGF aa,bb,ab TGF b1,b2 VEGF EGF ikassem@dr.com

Days of operation ikassem@dr.com

7 days later PLT exhausted after 7 days Macrophage by low oxygen tension, acidity, lactate concentration Secrete same growth factors Activated until it is down regulated by the restoration of normal oxygen tension ikassem@dr.com

Day 3rd ikassem@dr.com

17days Graft is completely permeated by new capillaries Initial osteoid formation Osteoclast resorb osteoid and BMP released New bone formation and maturation ikassem@dr.com

Days 14 ikassem@dr.com

The mechanism of growth factor effects on cells Inactive state ikassem@dr.com

Activation activation ikassem@dr.com

Clinical effects of PRP on bone regeneration Platelet RBC Normal blood PRP ikassem@dr.com

Trabecular areaNative mandible Autogenous BG ikassem@dr.com

Clinical effects of PRP on soft tissue healing Split thickness skin graft donor site healing ikassem@dr.com

6 days Thrombin side : 5% epithelization 95% granulation tissue PRP side ; 95% epithelization 5% granulation tissue ikassem@dr.com

6 months Thrombin side : scarring hypo and hyper- pigmentation area PRP side ;less scarring Much less pigmentation change ikassem@dr.com

Patient discomfort Patient discomfort decrease ikassem@dr.com

Epithelization ikassem@dr.com

6days ikassem@dr.com

1 months ikassem@dr.com

Contraindications Pre-existing coagulation defect : thrombocytopenia, hypofibrinogenia Hypersensitivity to bovine thrombin ikassem@dr.com

Clinical steps ikassem@dr.com

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Clinical application ikassem@dr.com

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Ridge preservation ikassem@dr.com

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