PCR & Molecular Biology...6DUVWHGW TXDOLW\ V\PEROV IRU ODERUDWRU\ SURGXFWV 7KH SLSHWWH WLS _ D...

21
Liquid Handling PCR & Molecular Biology

Transcript of PCR & Molecular Biology...6DUVWHGW TXDOLW\ V\PEROV IRU ODERUDWRU\ SURGXFWV 7KH SLSHWWH WLS _ D...

Page 1: PCR & Molecular Biology...6DUVWHGW TXDOLW\ V\PEROV IRU ODERUDWRU\ SURGXFWV 7KH SLSHWWH WLS _ D FULWLFDO IDFWRU LQ SUHFLVH GRVLQJ PCR Performance Tested Quality • The Sarstedt quality

Liquid Handling

PCR & Molecular Biology

Page 2: PCR & Molecular Biology...6DUVWHGW TXDOLW\ V\PEROV IRU ODERUDWRU\ SURGXFWV 7KH SLSHWWH WLS _ D FULWLFDO IDFWRU LQ SUHFLVH GRVLQJ PCR Performance Tested Quality • The Sarstedt quality

®

®

Compatibility table

®

®

®

®

Compatibility table

®

Your Sarstedt Team

Page 3: PCR & Molecular Biology...6DUVWHGW TXDOLW\ V\PEROV IRU ODERUDWRU\ SURGXFWV 7KH SLSHWWH WLS _ D FULWLFDO IDFWRU LQ SUHFLVH GRVLQJ PCR Performance Tested Quality • The Sarstedt quality

PCR Performance Tested Quality • The Sarstedt quality and purity standard

PCR Performance Tested

✓ DNA-free ✓ DNase/RNase-free ✓ PCR inhibitor-free

Human DNA < 0.5 pg/μl · Bacterial DNA < 0.02 pg/μl · DNase < 1x10-5 U/μlRNase < 1x10-9 Kunitz units/μl

Biosphere® plus quality • Unrivalled purity

®

®

®

Biosphere® plus

✓ Sterile ✓ DNA-free ✓ DNase/RNase-free✓ PCR inhibitor-free ✓ ATP-free ✓ Non-pyrogenic /endotoxin-free

Human DNA < 5.0 fg/μl · Bacterial DNA < 0.2 fg/μl · DNase < 5x10-7 U/μlRNase < 5x10-11 Kunitz units/μl · ATP < 1x10-12 mmol/μl · Pyrogens < 0.002 EU/mlSterility validated in accordance with ISO 11135

®

Conformity testing in accordance with the international test standard ISO 8655-2

Page 4: PCR & Molecular Biology...6DUVWHGW TXDOLW\ V\PEROV IRU ODERUDWRU\ SURGXFWV 7KH SLSHWWH WLS _ D FULWLFDO IDFWRU LQ SUHFLVH GRVLQJ PCR Performance Tested Quality • The Sarstedt quality

® ®

®

Piston

Pipette shaft

Aerosol

Aerosol-free

Filter

Sample

®

®

®

Page 5: PCR & Molecular Biology...6DUVWHGW TXDOLW\ V\PEROV IRU ODERUDWRU\ SURGXFWV 7KH SLSHWWH WLS _ D FULWLFDO IDFWRU LQ SUHFLVH GRVLQJ PCR Performance Tested Quality • The Sarstedt quality

Tailored tip solutions for every application •

Page 6: PCR & Molecular Biology...6DUVWHGW TXDOLW\ V\PEROV IRU ODERUDWRU\ SURGXFWV 7KH SLSHWWH WLS _ D FULWLFDO IDFWRU LQ SUHFLVH GRVLQJ PCR Performance Tested Quality • The Sarstedt quality

®

••

Tip:to easily identify which

tip is suitable for your pipette, use the compatibility table on

pages 18-21or at www.sarstedt.com

Page 7: PCR & Molecular Biology...6DUVWHGW TXDOLW\ V\PEROV IRU ODERUDWRU\ SURGXFWV 7KH SLSHWWH WLS _ D FULWLFDO IDFWRU LQ SUHFLVH GRVLQJ PCR Performance Tested Quality • The Sarstedt quality

®

Product(Illustrations: actual size)

Tray color code Order no. Volume Purity

Grade Packaging

Type B

Product Tray color code Order no. Volume Packaging

Type N

Type J

Type A

Type L

Type B

Type E

Type E

Type E

Type N

Type J

Type A

Type A

Type C

Type L

A0

1

2

3

4

5

loss

(rel

. uni

ts)

AB B C DSARSTEDT

Page 8: PCR & Molecular Biology...6DUVWHGW TXDOLW\ V\PEROV IRU ODERUDWRU\ SURGXFWV 7KH SLSHWWH WLS _ D FULWLFDO IDFWRU LQ SUHFLVH GRVLQJ PCR Performance Tested Quality • The Sarstedt quality

Order no. Design Packaging Pcs./case

Simple and practical

Economical and environmentally friendly

Tip StackPack packaging

reducing packaging and storage space required in the Tip SystemBox

Ecen

TippEmpty 96-tip reload trays and bulk-bagged tipsSuitable for manually racking bulk tips for autoclaving and convenient handling.

Order no. Tray color code Tip type/volume Packaging Pcs./case

Order no. Volume Purity Grade Packaging

Page 9: PCR & Molecular Biology...6DUVWHGW TXDOLW\ V\PEROV IRU ODERUDWRU\ SURGXFWV 7KH SLSHWWH WLS _ D FULWLFDO IDFWRU LQ SUHFLVH GRVLQJ PCR Performance Tested Quality • The Sarstedt quality

Filter tips(Illustrations: actual size)

Tray color code Order no. Max. sample

volume/colorPurity Grade Packaging

Type E

Type E

Type N

Type J

Type A

Type A

Type C

Type L

illustration 1:2

Type M

Tips without filters(Illustrations: actual size)

Tray color code Order no. Max. sample

volume/colorPurity Grade Packaging

Type E

Type N

Type J

Type A

Gelloaderillustration 1:2

Type C

Type L

illustration 1:2

Type MType B

illustration 1:2

illustration 1:2

illustration 1:2

Type B

illustration 1:2

Type O

tttioiiioion 1:2

Extra long

illustration 1:2

Type O

ratattttiiion 1:2

Extra long

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● = compatible 10 = limited pipetting volume ✗ = not recommended

= not checked MC = multi-channel pipette

® ® ® ®

® ® ® ®

Xplor

er®

® ®®

Bio

Cont

rol

®

0.1-

2.5

μl

0.5-

10 μ

l

2-20

μl

2-20

μl

50-2

00 μ

l

100-

1,00

0 μl

0.1-

2.5

μl

100-

1,00

0 μl

1-10

ml

0.1-

2.5

μl

100-

1,00

0 μl

1-10

ml

0.5-

10 μ

l

50-1

000

μl

100-

5000

μl

100-

1,00

0 μl

200-

1,00

0 μl

1-10

μl

30-3

00 μ

l

100-

1,00

0 μl

30-3

00 μ

l

100-

1,00

0 μl

P10/

U10

0.1-

P100

/U10

0

P200

/U20

020

-200

μl

P1.0

00/U

3.00

020

0-1,

000

μlP5

.000

/U5.

000

1000

-500

0 μl

P10/

U10

1-10

ml

● ●2.5

●2.5 ● ●

2.5 ● ●2.5

●2.5

●2.5

●2.5 ● ●

2.5●2.5 ● ●

2.5●2.5 ● ●

2.5 ✗

● ●10 ✗ ● ✗ ● ●

10 ● ● ● ● ● ● ● ● ● ✗

● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ✗

● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ✗

● ●20

●20 ✗ ●

20●20 ✗ ● ●

20 ● ✗ ●20

●20 ● ●

20 ✗ ● ●20

● ● ●100 ● ● ●

100 ✗ ● ● ●100 ✗ ● ● ● ●

100●

100 ✗ ✗ ● ●

● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ●

✗ ✗ ● ✗ ✗ ● ✗ ✗ ✗ ● ✗ ●200

●200 ✗ ✗ ● ●

200●

200●

200 ✗ ✗ ● ✗

✗ ✗ ● ✗ ✗ ● ● ● ● ● ● ✗ ✗ ● ● ● ● ✗ ✗ ● ●

● ● ● ● ✗ ● ● ● ● ●

● ● ● ● ● ● ● ● ● ●

® ® ® ®

Xplor

er®

® ®®

Bio

Cont

rol

®

● ● ● ● ● ● ● ●10 ● ● ● ● ● ● ● ● ● ● ● ✗

● ● ● ✗ ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ✗

● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ✗

● ● ● ● ● ● ✗ ● ● ● ✗ ● ● ● ● ● ●200 ✗ ● ● ● ●

200

● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ●

● ● ● ● ✗ ● ● ✗ ●250

●250 ✗ ✗ ● ●

250 ✗ ✗ ● ●250

✗ ✗ ● ✗ ✗ ● ● ✗ ✗ ● ● ✗ ✗ ● ● ✗ ✗ ● ● ● ● ● ● ✗ ✗ ● ●

● ● ● ● ✗ ● ● ● ● ✗ ●

● ● ● ● ● ● ● ● ● ●

5 m

l

✗ ✗ ✗ ●

5 m

l

● ● ● ✗

10 m

l

● ● ●

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® ® ® ® ®® ® ®

®

A. 8

35/

935e

®

● ● ● ● ● ● ● ● ✗ ● ● ● ● ● ● ● ● ● ● ● ●

● ● ● ● ● ● ●

● ● ● ● ● ✗ ● ● ✗ ● ● ● ● ● ● ● ● ✗ ● ●

✗ ● ✗ ● ● ✗ ✗ ✗ ✗ ● ● ● ✗ ✗ ● ● ● ✗ ● ● ● ● ● ● ● ● ● ● ● ● ● ●

● ● ● ● ● ● ● ● ●

✗ ● ● ● ● ● ●250 ✗ ● ● ● ✗ ✗ ● ● ● ✗ ● ✗ ✗ ✗ ● ✗ ✗ ✗ ✗ ✗ ✗ ● ✗ ●

✗ ● ● ✗ ✗ ● ● ● ● ✗ ✗ ✗ ● ● ✗ ✗ ● ✗ ● ✗ ✗ ✗ ● ✗ ✗ ✗ ✗ ● ● ● ✗ ●

● ● ● ✗ ● ● ● ● ● ●

● ● ✗ ● ● ● ● ● ●

5 m

l

✗ ✗ ✗ ✗

5 m

l

● ● ● ●

10 m

l

● ✗

® ® ®

® ® ® ® ®® ® ®

®

A. 8

35/

935e

®

0.5-

10 μ

l

20-2

00 μ

l

100-

1,00

0 μl

100-

5,00

0 μl

0.1-

2.5

μl

0.5-

10 μ

l

100-

1,00

0 μl

100-

5,00

0 μl

0.2-

10 μ

l

50-1

,000

μl

0.1-

1 μl

0.5-

10 μ

l

2-20

μl

2-20

μl

20-2

00 μ

l

100-

1,00

0 μl

0.1-

1 μl

0.5-

10 μ

l

20-2

00 μ

l

100-

1,00

0 μl

1-10

ml

0.5-

10 μ

l

100-

1,00

0 μl

1-10

μl

1-10

μl

2-20

μl

20-2

00 μ

l

100-

1,00

0 μl

1-10

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00 μ

l

0.1-

2 μl

0.5-

10 μ

l

1-10

μl

2-20

μl

100-

1,00

0 μl

0.5-

5 m

l

●2.5

●2.5 ● ●

2.5●2.5 ✗ ●

2.5●2.5 ✗ ●

2.5●2.5

●2.5

●2.5

●2.5

●2.5 ● ●

2.5●2.5 ● ✗ ●

2.5

● ● ● ● ✗ ● ●10 ✗ ● ● ● ● ● ● ● ● ● ● ● ●

● ● ● ✗ ● ● ● ●

● ● ● ● ● ✗ ● ● ✗ ● ● ● ● ● ● ✗ ✗ ✗ ● ●

✗ ✗ ✗ ●20

●20 ✗ ✗ ✗ ✗ ● ● ●

20 ✗ ✗ ● ●20

●20 ✗ ●

20●10 ● ●

20 ✗ ● ✗ ● ● ●20

●20 ✗ ● ✗

✗ ●100 ● ● ●

100 ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ●100 ✗ ●

100 ✗ ✗ ✗ ●100 ✗ ● ✗ ✗ ● ● ●

100 ● ●100

● ● ● ● ● ● ● ● ●

✗ ● ✗ ✗ ● ●200

●200 ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ● ✗ ●

100 ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ● ✗ ●

✗ ● ● ✗ ✗ ● ● ● ● ✗ ✗ ✗ ● ● ✗ ✗ ● ✗ ● ✗ ✗ ✗ ● ✗ ✗ ✗ ✗ ✗ ✗ ● ✗ ●

● ● ● ✗ ✗ ● ● ● ● ●

● ● ✗ ✗ ● ● ● ● ●

● = compatible 10 = limited pipetting volume ✗ = not recommended

= not checked MC = multi-channel pipette

Page 12: PCR & Molecular Biology...6DUVWHGW TXDOLW\ V\PEROV IRU ODERUDWRU\ SURGXFWV 7KH SLSHWWH WLS _ D FULWLFDO IDFWRU LQ SUHFLVH GRVLQJ PCR Performance Tested Quality • The Sarstedt quality

® ®

®

®

Can be opened without touching the inside of the lid

®

Product Order no. Vol. Color Quality Grade Units/bag Units/case

®

Product Order no. Multiply® μStrip, suitable for Color Quality Grade Units/bag

Units/case

®

Product Order no. Vol. Profile Color Lid strip Quality Grade

Units/bag Units/case

®

Page 13: PCR & Molecular Biology...6DUVWHGW TXDOLW\ V\PEROV IRU ODERUDWRU\ SURGXFWV 7KH SLSHWWH WLS _ D FULWLFDO IDFWRU LQ SUHFLVH GRVLQJ PCR Performance Tested Quality • The Sarstedt quality

®

®

Product Order no. Product description Design Packaging unit

®

t

Fluo

resc

ence

Transparent wells

White wells

CyclesCtCt

Number of plasmid copies

Ct values for the detection of plasmid copies in white and transparent plates at reduced reaction volumes

Ct v

alue

s

®

®

Order no. Capacity Format Suitable for Packaging

• Fluorescence level 10x higher• Greater sensitivity• Improved reproducibility

Page 14: PCR & Molecular Biology...6DUVWHGW TXDOLW\ V\PEROV IRU ODERUDWRU\ SURGXFWV 7KH SLSHWWH WLS _ D FULWLFDO IDFWRU LQ SUHFLVH GRVLQJ PCR Performance Tested Quality • The Sarstedt quality

Multiply®

®

Order no. Colour Skirt Deck Profile Well Max. volume Units/bag Units/case

®

®

Order no. Color Skirt Deck Profile Well Max. volume Blister packs/box

Order no. Color Skirt Deck Profile Well Max. volume Units/bag Units/case

Order no. Color Skirt Deck Profile Well Max. volume Units/bag Units/caseUnits/bag

Order no. Color Skirt Deck Profile Well Max. volume Units/bag Units/case

Tip: to easily identify which

plate is suitable for your thermocycler use our

compatibility table on page 30/31 or at

www.sarstedt.com.

Order no. Color Skirt Deck Profile Well Max. volume Units/bag Units/case

Page 15: PCR & Molecular Biology...6DUVWHGW TXDOLW\ V\PEROV IRU ODERUDWRU\ SURGXFWV 7KH SLSHWWH WLS _ D FULWLFDO IDFWRU LQ SUHFLVH GRVLQJ PCR Performance Tested Quality • The Sarstedt quality

®

Order no. Color Skirt Deck Well Max. volume Units/bag Units/case

Product Order no. Description Design Color Units/bag Units/case

Order no. Color Skirt Deck Profile Well Max. volume Units/bag Units/case

Order no. Color Skirt Deck Well Max. volume Units/bag Units/case

Order no. Color Skirt Deck Profile Well Max. volume Units/bag Units/case

Page 16: PCR & Molecular Biology...6DUVWHGW TXDOLW\ V\PEROV IRU ODERUDWRU\ SURGXFWV 7KH SLSHWWH WLS _ D FULWLFDO IDFWRU LQ SUHFLVH GRVLQJ PCR Performance Tested Quality • The Sarstedt quality

® ®

Number of wells 96 pre-inserted 96 96 96 96 384 full skirtHalf/full skirt Without Without Half Full Half Full

High High High Low Low -

Order no. PCR plates

Amersham Biosciences® / GE Healthcare®

Analytik Jena®/Biometra®

● ● ●

● ● ●

● ●

● ● ●

● ● ●

● ●

Applied Biosystems®/Life Technologies®

® ● ●® ● ●® ● ● ●® ● ● ●® ●

● ●

● ● ●

● ● ●® ● ●® ● ●® ●® ●® ● ●® ●® ● ●

Veriti® ● ●

Veriti® ● ●

● ● ●

● ● ●

● ●

● ● ●

PeqLab®

● ● ●

®

● ● ●

● ●

Number of wells 96 pre-inserted 96 96 96 96 384 full skirt 96-well 384-wellHalf/full skirt Without Without Half Full Half Full Full Full

High High High Low Low - ‘Lightcycler’ ‘Lightcycler’

Order no. PCR plates

BioRad®/MJ Research®

● ● ●

● ● ● ●

● ● ● ●

● ● ●

● ● ●

● ● ●

● ● ●

● ●

Corbett Research®/Qiagen®

Eppendorf®

® ● ●® ● ● ●® ● ● ●® ● ● ● ●® ● ● ●

Ericom®

● ●

● ●

● ●

MWG®

● ●

● ● ●

Roche®

® ●® ● ●

Stratagene®/Agilent®

● ●

● ● ● ●

● ● ●

● ●

Techne®

● ●

● ● ●

● ● ●

● ●

● ● ● ●

● ● ●

● ●

● ● ●

● ● ● ●

Page 17: PCR & Molecular Biology...6DUVWHGW TXDOLW\ V\PEROV IRU ODERUDWRU\ SURGXFWV 7KH SLSHWWH WLS _ D FULWLFDO IDFWRU LQ SUHFLVH GRVLQJ PCR Performance Tested Quality • The Sarstedt quality

95.1999

95.1994

95.1993

95.1995

Order no. Product description Application Special properties Clear Pierceable Functional

temperature range Packaging

+

+

+

+

+

Film/foil properties Order no.

Page 18: PCR & Molecular Biology...6DUVWHGW TXDOLW\ V\PEROV IRU ODERUDWRU\ SURGXFWV 7KH SLSHWWH WLS _ D FULWLFDO IDFWRU LQ SUHFLVH GRVLQJ PCR Performance Tested Quality • The Sarstedt quality

✓ ✓ ✓

BS

A lo

ss (%

)

Standard tube Tubes with low binding properties

SupplierSarstedt

Experiment method:

Sarstedt

Supplier AControl

Supplier B

Supplier C

Supplier D

Fluo

resc

ence

Cycle403938373635343332313029282726252423222120191817161514131211109876543210

100

1000

10000

Sarstedt

B C DAControl

Volume Order no. Description Packaging

®

Volume Order no. Purity Grade Packaging

Page 19: PCR & Molecular Biology...6DUVWHGW TXDOLW\ V\PEROV IRU ODERUDWRU\ SURGXFWV 7KH SLSHWWH WLS _ D FULWLFDO IDFWRU LQ SUHFLVH GRVLQJ PCR Performance Tested Quality • The Sarstedt quality

Order no. Height in mm LCH Clear sides Units/Styrofoam rack/case

®

®

Order no. Design Units/bag Units/case

®

®

®

®

®

Order no. Design Volume/material Units/bag Units/case

® ®

Wavelength (nm)

Transmission (T) in %

UV Acrylic Polystyrene

Page 20: PCR & Molecular Biology...6DUVWHGW TXDOLW\ V\PEROV IRU ODERUDWRU\ SURGXFWV 7KH SLSHWWH WLS _ D FULWLFDO IDFWRU LQ SUHFLVH GRVLQJ PCR Performance Tested Quality • The Sarstedt quality
Page 21: PCR & Molecular Biology...6DUVWHGW TXDOLW\ V\PEROV IRU ODERUDWRU\ SURGXFWV 7KH SLSHWWH WLS _ D FULWLFDO IDFWRU LQ SUHFLVH GRVLQJ PCR Performance Tested Quality • The Sarstedt quality

Screw Cap Micro TubesTransport, storage and sample preparation

Inner sub-heading 14ptCell and tissue cultureCell Cultivation, Cryogenic Storage, Filtration, Liquid Handling

• Designed to accommodate a range of tube sizes in one rack• • Units are rotatable and freely exchangeable• Ideal for use in the temperature range from -80°C to 121°C• L x W x H (per unit): 108 x 108 x 257 (108 x 108 x 69) mm

Ordering Information

Art. No. Description Packaging

93.879 Revolver Workstation1/bag4/case

Revolver Workstation

SARSTEDT AG & Co.P.O. Box 12 20D-51582 NümbrechtPhone +49 2293 305-0Fax +49 2293 [email protected]

ELISA PlatesMicro Test Plates for Immunoanalytics

IVARO Tube Handler XSInnovative automation for tube processing

MicrobiologySample collection, cultivation, processing

1