PCR & Molecular Biology...6DUVWHGW TXDOLW\ V\PEROV IRU ODERUDWRU\ SURGXFWV 7KH SLSHWWH WLS _ D...
Transcript of PCR & Molecular Biology...6DUVWHGW TXDOLW\ V\PEROV IRU ODERUDWRU\ SURGXFWV 7KH SLSHWWH WLS _ D...
Liquid Handling
PCR & Molecular Biology
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Compatibility table
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Compatibility table
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Your Sarstedt Team
PCR Performance Tested Quality • The Sarstedt quality and purity standard
PCR Performance Tested
✓ DNA-free ✓ DNase/RNase-free ✓ PCR inhibitor-free
Human DNA < 0.5 pg/μl · Bacterial DNA < 0.02 pg/μl · DNase < 1x10-5 U/μlRNase < 1x10-9 Kunitz units/μl
Biosphere® plus quality • Unrivalled purity
®
®
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Biosphere® plus
✓ Sterile ✓ DNA-free ✓ DNase/RNase-free✓ PCR inhibitor-free ✓ ATP-free ✓ Non-pyrogenic /endotoxin-free
Human DNA < 5.0 fg/μl · Bacterial DNA < 0.2 fg/μl · DNase < 5x10-7 U/μlRNase < 5x10-11 Kunitz units/μl · ATP < 1x10-12 mmol/μl · Pyrogens < 0.002 EU/mlSterility validated in accordance with ISO 11135
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Conformity testing in accordance with the international test standard ISO 8655-2
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Piston
Pipette shaft
Aerosol
Aerosol-free
Filter
Sample
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Tailored tip solutions for every application •
•
•
•
•
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•
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Tip:to easily identify which
tip is suitable for your pipette, use the compatibility table on
pages 18-21or at www.sarstedt.com
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Product(Illustrations: actual size)
Tray color code Order no. Volume Purity
Grade Packaging
Type B
Product Tray color code Order no. Volume Packaging
Type N
Type J
Type A
Type L
Type B
Type E
Type E
Type E
Type N
Type J
Type A
Type A
Type C
Type L
A0
1
2
3
4
5
loss
(rel
. uni
ts)
AB B C DSARSTEDT
Order no. Design Packaging Pcs./case
Simple and practical
Economical and environmentally friendly
Tip StackPack packaging
reducing packaging and storage space required in the Tip SystemBox
Ecen
TippEmpty 96-tip reload trays and bulk-bagged tipsSuitable for manually racking bulk tips for autoclaving and convenient handling.
Order no. Tray color code Tip type/volume Packaging Pcs./case
Order no. Volume Purity Grade Packaging
Filter tips(Illustrations: actual size)
Tray color code Order no. Max. sample
volume/colorPurity Grade Packaging
Type E
Type E
Type N
Type J
Type A
Type A
Type C
Type L
illustration 1:2
Type M
Tips without filters(Illustrations: actual size)
Tray color code Order no. Max. sample
volume/colorPurity Grade Packaging
Type E
Type N
Type J
Type A
Gelloaderillustration 1:2
Type C
Type L
illustration 1:2
Type MType B
illustration 1:2
illustration 1:2
illustration 1:2
Type B
illustration 1:2
Type O
tttioiiioion 1:2
Extra long
illustration 1:2
Type O
ratattttiiion 1:2
Extra long
● = compatible 10 = limited pipetting volume ✗ = not recommended
= not checked MC = multi-channel pipette
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Xplor
er®
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Bio
Cont
rol
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0.1-
2.5
μl
0.5-
10 μ
l
2-20
μl
2-20
μl
50-2
00 μ
l
100-
1,00
0 μl
0.1-
2.5
μl
100-
1,00
0 μl
1-10
ml
0.1-
2.5
μl
100-
1,00
0 μl
1-10
ml
0.5-
10 μ
l
50-1
000
μl
100-
5000
μl
100-
1,00
0 μl
200-
1,00
0 μl
1-10
μl
30-3
00 μ
l
100-
1,00
0 μl
30-3
00 μ
l
100-
1,00
0 μl
P10/
U10
0.1-
P100
/U10
0
P200
/U20
020
-200
μl
P1.0
00/U
3.00
020
0-1,
000
μlP5
.000
/U5.
000
1000
-500
0 μl
P10/
U10
1-10
ml
● ●2.5
●2.5 ● ●
2.5 ● ●2.5
●2.5
●2.5
●2.5 ● ●
2.5●2.5 ● ●
2.5●2.5 ● ●
2.5 ✗
● ●10 ✗ ● ✗ ● ●
10 ● ● ● ● ● ● ● ● ● ✗
● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ✗
● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ✗
● ●20
●20 ✗ ●
20●20 ✗ ● ●
20 ● ✗ ●20
●20 ● ●
20 ✗ ● ●20
● ● ●100 ● ● ●
100 ✗ ● ● ●100 ✗ ● ● ● ●
100●
100 ✗ ✗ ● ●
● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ●
✗ ✗ ● ✗ ✗ ● ✗ ✗ ✗ ● ✗ ●200
●200 ✗ ✗ ● ●
200●
200●
200 ✗ ✗ ● ✗
✗ ✗ ● ✗ ✗ ● ● ● ● ● ● ✗ ✗ ● ● ● ● ✗ ✗ ● ●
● ● ● ● ✗ ● ● ● ● ●
● ● ● ● ● ● ● ● ● ●
® ® ® ®
Xplor
er®
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Bio
Cont
rol
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● ● ● ● ● ● ● ●10 ● ● ● ● ● ● ● ● ● ● ● ✗
● ● ● ✗ ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ✗
● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ✗
● ● ● ● ● ● ✗ ● ● ● ✗ ● ● ● ● ● ●200 ✗ ● ● ● ●
200
● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ●
● ● ● ● ✗ ● ● ✗ ●250
●250 ✗ ✗ ● ●
250 ✗ ✗ ● ●250
✗ ✗ ● ✗ ✗ ● ● ✗ ✗ ● ● ✗ ✗ ● ● ✗ ✗ ● ● ● ● ● ● ✗ ✗ ● ●
● ● ● ● ✗ ● ● ● ● ✗ ●
● ● ● ● ● ● ● ● ● ●
5 m
l
✗ ✗ ✗ ●
5 m
l
● ● ● ✗
10 m
l
● ● ●
® ® ® ® ®® ® ®
®
A. 8
35/
935e
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● ● ● ● ● ● ● ● ✗ ● ● ● ● ● ● ● ● ● ● ● ●
● ● ● ● ● ● ●
● ● ● ● ● ✗ ● ● ✗ ● ● ● ● ● ● ● ● ✗ ● ●
✗ ● ✗ ● ● ✗ ✗ ✗ ✗ ● ● ● ✗ ✗ ● ● ● ✗ ● ● ● ● ● ● ● ● ● ● ● ● ● ●
● ● ● ● ● ● ● ● ●
✗ ● ● ● ● ● ●250 ✗ ● ● ● ✗ ✗ ● ● ● ✗ ● ✗ ✗ ✗ ● ✗ ✗ ✗ ✗ ✗ ✗ ● ✗ ●
✗ ● ● ✗ ✗ ● ● ● ● ✗ ✗ ✗ ● ● ✗ ✗ ● ✗ ● ✗ ✗ ✗ ● ✗ ✗ ✗ ✗ ● ● ● ✗ ●
● ● ● ✗ ● ● ● ● ● ●
● ● ✗ ● ● ● ● ● ●
5 m
l
✗ ✗ ✗ ✗
5 m
l
● ● ● ●
10 m
l
● ✗
® ® ®
® ® ® ® ®® ® ®
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A. 8
35/
935e
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0.5-
10 μ
l
20-2
00 μ
l
100-
1,00
0 μl
100-
5,00
0 μl
0.1-
2.5
μl
0.5-
10 μ
l
100-
1,00
0 μl
100-
5,00
0 μl
0.2-
10 μ
l
50-1
,000
μl
0.1-
1 μl
0.5-
10 μ
l
2-20
μl
2-20
μl
20-2
00 μ
l
100-
1,00
0 μl
0.1-
1 μl
0.5-
10 μ
l
20-2
00 μ
l
100-
1,00
0 μl
1-10
ml
0.5-
10 μ
l
100-
1,00
0 μl
1-10
μl
1-10
μl
2-20
μl
20-2
00 μ
l
100-
1,00
0 μl
1-10
μl
20-2
00 μ
l
0.1-
2 μl
0.5-
10 μ
l
1-10
μl
2-20
μl
100-
1,00
0 μl
0.5-
5 m
l
●2.5
●2.5 ● ●
2.5●2.5 ✗ ●
2.5●2.5 ✗ ●
2.5●2.5
●2.5
●2.5
●2.5
●2.5 ● ●
2.5●2.5 ● ✗ ●
2.5
● ● ● ● ✗ ● ●10 ✗ ● ● ● ● ● ● ● ● ● ● ● ●
● ● ● ✗ ● ● ● ●
● ● ● ● ● ✗ ● ● ✗ ● ● ● ● ● ● ✗ ✗ ✗ ● ●
✗ ✗ ✗ ●20
●20 ✗ ✗ ✗ ✗ ● ● ●
20 ✗ ✗ ● ●20
●20 ✗ ●
20●10 ● ●
20 ✗ ● ✗ ● ● ●20
●20 ✗ ● ✗
✗ ●100 ● ● ●
100 ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ●100 ✗ ●
100 ✗ ✗ ✗ ●100 ✗ ● ✗ ✗ ● ● ●
100 ● ●100
● ● ● ● ● ● ● ● ●
✗ ● ✗ ✗ ● ●200
●200 ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ● ✗ ●
100 ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ✗ ● ✗ ●
✗ ● ● ✗ ✗ ● ● ● ● ✗ ✗ ✗ ● ● ✗ ✗ ● ✗ ● ✗ ✗ ✗ ● ✗ ✗ ✗ ✗ ✗ ✗ ● ✗ ●
● ● ● ✗ ✗ ● ● ● ● ●
● ● ✗ ✗ ● ● ● ● ●
● = compatible 10 = limited pipetting volume ✗ = not recommended
= not checked MC = multi-channel pipette
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Can be opened without touching the inside of the lid
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Product Order no. Vol. Color Quality Grade Units/bag Units/case
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Product Order no. Multiply® μStrip, suitable for Color Quality Grade Units/bag
Units/case
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Product Order no. Vol. Profile Color Lid strip Quality Grade
Units/bag Units/case
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Product Order no. Product description Design Packaging unit
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t
Fluo
resc
ence
Transparent wells
White wells
CyclesCtCt
Number of plasmid copies
Ct values for the detection of plasmid copies in white and transparent plates at reduced reaction volumes
Ct v
alue
s
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Order no. Capacity Format Suitable for Packaging
• Fluorescence level 10x higher• Greater sensitivity• Improved reproducibility
Multiply®
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Order no. Colour Skirt Deck Profile Well Max. volume Units/bag Units/case
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®
Order no. Color Skirt Deck Profile Well Max. volume Blister packs/box
Order no. Color Skirt Deck Profile Well Max. volume Units/bag Units/case
Order no. Color Skirt Deck Profile Well Max. volume Units/bag Units/caseUnits/bag
Order no. Color Skirt Deck Profile Well Max. volume Units/bag Units/case
Tip: to easily identify which
plate is suitable for your thermocycler use our
compatibility table on page 30/31 or at
www.sarstedt.com.
Order no. Color Skirt Deck Profile Well Max. volume Units/bag Units/case
®
Order no. Color Skirt Deck Well Max. volume Units/bag Units/case
Product Order no. Description Design Color Units/bag Units/case
Order no. Color Skirt Deck Profile Well Max. volume Units/bag Units/case
Order no. Color Skirt Deck Well Max. volume Units/bag Units/case
Order no. Color Skirt Deck Profile Well Max. volume Units/bag Units/case
® ®
Number of wells 96 pre-inserted 96 96 96 96 384 full skirtHalf/full skirt Without Without Half Full Half Full
High High High Low Low -
Order no. PCR plates
Amersham Biosciences® / GE Healthcare®
●
●
Analytik Jena®/Biometra®
●
● ● ●
● ● ●
● ●
● ● ●
● ● ●
●
● ●
●
Applied Biosystems®/Life Technologies®
® ● ●® ● ●® ● ● ●® ● ● ●® ●
● ●
● ● ●
● ● ●® ● ●® ● ●® ●® ●® ● ●® ●® ● ●
●
●
Veriti® ● ●
Veriti® ● ●
●
● ● ●
● ● ●
● ●
● ● ●
PeqLab®
● ● ●
●
®
● ● ●
● ●
●
Number of wells 96 pre-inserted 96 96 96 96 384 full skirt 96-well 384-wellHalf/full skirt Without Without Half Full Half Full Full Full
High High High Low Low - ‘Lightcycler’ ‘Lightcycler’
Order no. PCR plates
BioRad®/MJ Research®
●
●
● ● ●
● ● ● ●
● ● ● ●
● ● ●
● ● ●
● ● ●
● ● ●
● ●
●
●
Corbett Research®/Qiagen®
●
●
Eppendorf®
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Ericom®
● ●
● ●
● ●
MWG®
● ●
●
● ● ●
Roche®
® ●® ● ●
Stratagene®/Agilent®
●
● ●
● ● ● ●
● ● ●
● ●
●
Techne®
● ●
● ● ●
● ● ●
● ●
● ● ● ●
●
● ● ●
● ●
● ● ●
● ● ● ●
95.1999
95.1994
95.1993
95.1995
Order no. Product description Application Special properties Clear Pierceable Functional
temperature range Packaging
+
+
+
+
+
Film/foil properties Order no.
✓ ✓ ✓
BS
A lo
ss (%
)
Standard tube Tubes with low binding properties
SupplierSarstedt
Experiment method:
Sarstedt
Supplier AControl
Supplier B
Supplier C
Supplier D
Fluo
resc
ence
Cycle403938373635343332313029282726252423222120191817161514131211109876543210
100
1000
10000
Sarstedt
B C DAControl
Volume Order no. Description Packaging
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Volume Order no. Purity Grade Packaging
Order no. Height in mm LCH Clear sides Units/Styrofoam rack/case
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Order no. Design Units/bag Units/case
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Order no. Design Volume/material Units/bag Units/case
® ®
Wavelength (nm)
Transmission (T) in %
UV Acrylic Polystyrene
Screw Cap Micro TubesTransport, storage and sample preparation
Inner sub-heading 14ptCell and tissue cultureCell Cultivation, Cryogenic Storage, Filtration, Liquid Handling
• Designed to accommodate a range of tube sizes in one rack• • Units are rotatable and freely exchangeable• Ideal for use in the temperature range from -80°C to 121°C• L x W x H (per unit): 108 x 108 x 257 (108 x 108 x 69) mm
Ordering Information
Art. No. Description Packaging
93.879 Revolver Workstation1/bag4/case
Revolver Workstation
SARSTEDT AG & Co.P.O. Box 12 20D-51582 NümbrechtPhone +49 2293 305-0Fax +49 2293 [email protected]
ELISA PlatesMicro Test Plates for Immunoanalytics
IVARO Tube Handler XSInnovative automation for tube processing
MicrobiologySample collection, cultivation, processing
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