pAMPK
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pAMPK β-act AMPK 1 10 50 Metformin (mM) 5’ 15’ 30’ Metformin (t) pAMPK β-act AMPK pNF-B NF-B β-act GAPDH 5’ 15’ 30’ Metformin (t) 60’ 24h A B C Ctrl Ctrl Ctrl Supplementary Fig. 1
description
Metformin (t). 30 ’. 5 ’. 15 ’. pAMPK. AMPK. β -act. Metformin (t). 30 ’. 60’. 24h. 5 ’. 15 ’. pNF- B. NF- B. β -act. GAPDH. Supplementary Fig. 1. A. Metformin (mM). Ctrl. 1. 10. 50. pAMPK. AMPK. β -act. B. Ctrl. C. Ctrl. ). %. (. n. o. i. t. a. l. u. p. - PowerPoint PPT Presentation
Transcript of pAMPK
pAMPK
β-act
AMPK
1 10 50
Metformin (mM)
5’ 15’ 30’
Metformin (t)
pAMPK
β-act
AMPK
pNF-B
NF-B
β-act
GAPDH
5’ 15’ 30’
Metformin (t)
60’ 24h
A
B
C Ctrl
Ctrl
Ctrl
Supplementary Fig. 1
AM
TT
(O
D5
40)
E
C
Pop
ulat
ion
(%)
NT 6h 24h 48hMetf 10mM
Pro-Caspase 3
β-act
C
NT 6h 24h 48hMetf 10mM
Cyclin D1
CDK4
Vinculin
Re
lativ
e
mR
NA
leve
ls
Metf [10mM]
- + +6h 24h
Cyclin D1 p21
Metf [10mM]
- + +6h 24h
Re
lativ
e
mR
NA
leve
ls
0
20
40
60
80
100
% C
E LLS
NECROSISLATE APOPTOSISEARLY APOPTOSISVIABLE
D
G1/G0
S
G2/M
Apop
Dead
B
Cyclin D1 CDK4
- 6 24 48 - 6 24 48h Metf
Re
lativ
e E
xpre
ssio
n
* * *
Supplementary Fig. 2
0
20
40
60
80
100
120
Popu
lati
o n (
%)
NT 6h 24h 48hMetf 10mM
0.1
Metf (mM)NT 1 5 10 20 50 100 25mM
5-FU
Supplementary Fig. 3
Supplementary Fig. 4
E
MCF-7
Internal Ctrl CCL-2
HUVEC
VEGF-C
