High-resolution Imaging of Debris Disks Jane Greaves St Andrews University, Scotland.
High Performance High Speed High Resolution microCT · 2016. 2. 15. · High Resolution Imaging at...
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Pre-clinical in vivo imaging PRODUCT NOTE Key Features: • High resolution (4.5 μm voxel size) • High speed (8 second scans) • Low dose imaging for longitudinal studies • Co-registration of molecular optical signals with anatomical microCT data • Two physical magnifications for a range of resolutions and doses • Two-phase respiratory and cardiac gating • Mouse/rat/rabbit imaging capabilities The Quantum GX is the most advanced microCT imaging system for preclinical research, offering industry leading resolution combined with high speed imaging capability at an X-ray dose low enough to enable true longitudinal imaging of animals. The Quantum GX is the only multispecies microCT system with the capability to image entire mice, rats and rabbits. This high resolution, high speed integrated platform enables researchers to gain a better understanding of disease in a broad range of applications in cardiovascular, respiratory, bone, lung and brain imaging research. Gain more insight into the molecular, functional and anatomical readouts of the experimental model by coregistering of 3D optical data from PerkinElmer's IVIS ® and FMT ® platforms with the Quantum GX microCT system for a better understanding of disease and its progression. High Performance High Speed High Resolution microCT Quantum GX
Transcript of High Performance High Speed High Resolution microCT · 2016. 2. 15. · High Resolution Imaging at...
Pre-clinical in vivo imaging
P R O D U C T N O T E
Key Features:
•Highresolution(4.5μmvoxelsize)
•Highspeed(8secondscans)
•Lowdoseimagingforlongitudinalstudies
•Co-registrationofmolecularopticalsignals withanatomicalmicroCTdata
•Twophysicalmagnificationsforarangeof resolutions and doses
•Two-phaserespiratoryandcardiacgating
•Mouse/rat/rabbitimagingcapabilities
TheQuantumGXisthemostadvancedmicroCTimaging systemforpreclinicalresearch,offeringindustryleadingresolutioncombinedwithhigh
speedimagingcapabilityatanX-raydoselowenoughtoenabletruelongitudinalimagingofanimals.TheQuantumGXistheonlymultispeciesmicroCTsystemwiththecapabilitytoimageentiremice,ratsandrabbits.
Thishighresolution,highspeedintegratedplatformenablesresearchers togainabetterunderstandingofdiseaseinabroadrangeofapplicationsincardiovascular,respiratory,bone,lungandbrainimagingresearch.
Gainmoreinsightintothemolecular,functionalandanatomicalreadouts oftheexperimentalmodelbycoregisteringof3DopticaldatafromPerkinElmer'sIVIS®andFMT®platformswiththeQuantumGXmicroCTsystem forabetterunderstandingofdiseaseanditsprogression.
High PerformanceHigh Speed High Resolution microCT
Quantum GX
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High Resolution Imaging at a 4.5 μm Voxel Size
TheQuantumGXoffersthehighestresolutionamongall themicroCTscannersforin vivoimaging.Thewidefieldofview(FOV)scanningat36mmand72mmallowsforhighresolutionimagingofmice,ratsandrabbits.Thesystemhasthreemodes:highresolution,highspeedandstandardmodes.Asanexample,inthehighresolutionmode,a4.5μmvoxelsizeresolutioncanbeattainedata36x36mmFOV,whilea 9μmvoxelsizeresolutioncanbeattainedat72x72mmFOV.
AnadvancedboneanalysissoftwarepackageisavailableasacompaniontotheQuantumGXthatofferssuperiorvisualizationtoolsforbonesegmentation,BMDmeasurementsandbonemorphologyassessment(trabecularandcorticalparameters)in auser-friendlyworkflow. Figure 1. Top: High resolution microCT image of mouse femur (4.5 micron voxel, 8k
x 8k pixels) Bottom: Heart and vasculature imaging with contrast agent, Cortical bone and bone segmentation images.
Figure 3. Top: High Speed Imaging: 8 second scan of mouse lung region and whole mouse scan in 24 seconds. Bottom: Low dose: Longitudinal imaging tibial osteolytic lesions casued by human breast cancer cell line MDA-MB-231.
High Speed/Low Dose Imaging
TheQuantumGXisthefastestmicroCTsystemwithindustryleadingscantimesof8secondsinthehighspeedmode. Withareconstructiontimeof15seconds,a3Dimagecan beacquiredandreconstructedwiththeGXin23seconds.
WiththeQuantumGX,followandcharacterizediseaseprogressionthroughouttheentirestudyusingmicroCTateveryimagingpoint.Usingthe8secondscantimethatisdesigned tosimultaneouslygivelowdoseandgoodimagequality,researcherscanbeconfidentthattheirbiologicalmodelwillremainrelevantthroughoutthespanoftheexperiment.Fastimagingandsmoothworkflowsalsoenablethethroughputrequiredtoscancohortsofanimalsquicklyanddrawsoundconclusionsfromexperimentaldata.
Superior Workflow for High Resolution Scans
TheQuantumGXimagingsystemfeaturesanadvancedworkflowforcreatinghighresolutionimagesfromtheoriginalwholeimagescan.Awholebodyscanisfirsttakenandthenregionsofinterests(ROIs)aredefinedtoreconstructahighresolutionimage.Sincethereisnoneedtorescantheanimal,thethroughputishigherandradiationexposureissignificantlyreduced.ThelargerfieldofviewscanoffersflexibilitytoselectanROIfordetailatalaterstage.SubvolumereconstructionswithvariousresolutionsettingscanbeperformedatanytimeusinganFOVdefinedbytheuser.
Figure 2. A 4.5 micron post reconstruction (bottom image) from initial image (top) which was taken in high resolution mode (FOV 36, 72 micron pixel size).
Day 7 Day 14 Day 21 Day 24
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Figure 5. Two-phase gating technique on the Quantum GX offers reconstructions with less motion artifacts.
FPO
Inspiration
Expiration
Two-Phase Cardiac and Respiratory Gating
ForaccuratemicroCTreconstructionincardiacandrespiratorygatedapplications,itisextremelyimportanttominimizethemotionfromthediaphragmandheart.TheQuantumGX’sadvancedandsimpleintrinsicretrospectivetwo-phasegatingtechniquesareideallysuitedforcardiacandlungfunctionmeasurements.Bysimplydrawingaregionofinterestoverthediaphragmand/orapexoftheheart,theoptionalsoftwarethenreprocessesthedata,usingonlytheviewsfromtheselectedsliceintherespiratoryorcardiaccycle,reducingmotionartifactsin the reconstruction.
Figure 4. Optical co-registration of luciferase labeled MDA-MB-231 metastases with microCT.
Optical Co-registration for Multimodality Imaging
PerkinElmerhasrevolutionizedpreclinical3DopticalimagingwiththeIVISandFMTplatforms.TheQuantumGXmakesmicroCTimagingaseasyasopticalimagingontheIVISandallowsresearcherstoco-registerfunctionalopticalsignalswithanatomicalmicroCTdata.Throughputanduserworkflowhavebeensimplifiedtoseamlesslyco-registeranatomicalandfunctionaldataateverystepofalongitudinalstudy.
Multispecies Imaging
TheQuantumGXfeaturesisatruemultispeciesmicroCTsystemwithaboresizelargeenoughtofitentiremice,ratsandevenrabbits.TheboresizeintheQuantumGXis163mm andwithavailablemouse,ratandrabbitbeds,animalsupto5kgcanbeeasilyimaged.Anentiremousecanbeimagedinonescan.Aratcanbeimagedintwosetsofscansandaguineapigcanbeimagedinthreesetsofscans.
FPO
Mouse
Rat
Figure 6. The Quantum GX is an ideal multispecies imaging system. The system can image entire mice, rats, guinea pigs and even rabbits.
GuineaPig
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Quantum GX High Performance microCT for Preclinical Applications
InjuredNormal
RatwithMIA-inducedosteoarthritisintheknee.Theimageontheleftshowsadiseasedjointcomparedtothenormaljoint on the right
OsteoarthritisNormal
NormalOsteoarthritis
Osteoarthritis Lung Injury
Contrast Agent Enhanced Imaging
Heartandpulmonaryvasculature Livervasculatureimaging 4T1tumorvasculature Lungsegmentation
Additional Applications• Bone research • Bonemorphology(trabecularandcorticalbone)
assessment,BMDmeasurements
• Cardiovascular disease • Myocardialviability,calciumscoring,ventricular
functionandmetabolism,infarcthealing
• Pulmonary disease • Lungairwaystructureandwholelunginacute
injurymodels
•Volume,densityandFRC(functionalresidual capacity)measurements
• Metabolic disease • Differentiationofvisceralandsubcutaneousfat
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Optional Accessories
Other Accessories
•AdvancedmicroCTanalysissoftware
•Cardiac/respiratorygatingsoftware
•3Dmultimodalitymodule
•Adapterarms(Spectrum,FMT)
•Rabbitbed
MouseImagingShuttle
IVISSyringeInjectionSystemXGI-8AnesthesiaSystem
Quantum GX Technical Specifications
CTimage
Fieldofview 72mm(max)
Resolution(pixelsize) 4.5μm(min)
Numberofpixels512×512×512- 8000×8000
X-raytube
Maximumtubevoltage 90kV
Maximumtubecurrent 200 μA
Maximumoutput 8 W
DetectorType Flat panel detector
FrameRate 60fps(max)
CTgantryBoreSize 163mm(max)
Scanablerange 240mm(max)
Scantimes
HighSpeed: 8sec Standard: 18sec,2min HighResolution: 4min, 14min,57min
ImagereconstructionMin.15sec@512×512pixels×512view
SoftwareAcquisition and visualizationpackage
Dimensions(HxWxD) 1450x980x930mm
Weight 450 kg
XWS-260Workbench
For more information, please visit www.perkinelmer.com/invivo
For research use only. Not for use in diagnostic procedures.
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