Pre-clinical in vivo imaging

P R O D U C T N O T E

Key Features:

•Highresolution(4.5μmvoxelsize)

•Highspeed(8secondscans)

•Lowdoseimagingforlongitudinalstudies

•Co-registrationofmolecularopticalsignals withanatomicalmicroCTdata

•Twophysicalmagnificationsforarangeof resolutions and doses

•Two-phaserespiratoryandcardiacgating

•Mouse/rat/rabbitimagingcapabilities

TheQuantumGXisthemostadvancedmicroCTimaging systemforpreclinicalresearch,offeringindustryleadingresolutioncombinedwithhigh

speedimagingcapabilityatanX-raydoselowenoughtoenabletruelongitudinalimagingofanimals.TheQuantumGXistheonlymultispeciesmicroCTsystemwiththecapabilitytoimageentiremice,ratsandrabbits.

Thishighresolution,highspeedintegratedplatformenablesresearchers togainabetterunderstandingofdiseaseinabroadrangeofapplicationsincardiovascular,respiratory,bone,lungandbrainimagingresearch.

Gainmoreinsightintothemolecular,functionalandanatomicalreadouts oftheexperimentalmodelbycoregisteringof3DopticaldatafromPerkinElmer'sIVIS®andFMT®platformswiththeQuantumGXmicroCTsystem forabetterunderstandingofdiseaseanditsprogression.

High PerformanceHigh Speed High Resolution microCT

Quantum GX

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High Resolution Imaging at a 4.5 μm Voxel Size

TheQuantumGXoffersthehighestresolutionamongall themicroCTscannersforin vivoimaging.Thewidefieldofview(FOV)scanningat36mmand72mmallowsforhighresolutionimagingofmice,ratsandrabbits.Thesystemhasthreemodes:highresolution,highspeedandstandardmodes.Asanexample,inthehighresolutionmode,a4.5μmvoxelsizeresolutioncanbeattainedata36x36mmFOV,whilea 9μmvoxelsizeresolutioncanbeattainedat72x72mmFOV.

AnadvancedboneanalysissoftwarepackageisavailableasacompaniontotheQuantumGXthatofferssuperiorvisualizationtoolsforbonesegmentation,BMDmeasurementsandbonemorphologyassessment(trabecularandcorticalparameters)in auser-friendlyworkflow. Figure 1. Top: High resolution microCT image of mouse femur (4.5 micron voxel, 8k

x 8k pixels) Bottom: Heart and vasculature imaging with contrast agent, Cortical bone and bone segmentation images.

Figure 3. Top: High Speed Imaging: 8 second scan of mouse lung region and whole mouse scan in 24 seconds. Bottom: Low dose: Longitudinal imaging tibial osteolytic lesions casued by human breast cancer cell line MDA-MB-231.

High Speed/Low Dose Imaging

TheQuantumGXisthefastestmicroCTsystemwithindustryleadingscantimesof8secondsinthehighspeedmode. Withareconstructiontimeof15seconds,a3Dimagecan beacquiredandreconstructedwiththeGXin23seconds.

WiththeQuantumGX,followandcharacterizediseaseprogressionthroughouttheentirestudyusingmicroCTateveryimagingpoint.Usingthe8secondscantimethatisdesigned tosimultaneouslygivelowdoseandgoodimagequality,researcherscanbeconfidentthattheirbiologicalmodelwillremainrelevantthroughoutthespanoftheexperiment.Fastimagingandsmoothworkflowsalsoenablethethroughputrequiredtoscancohortsofanimalsquicklyanddrawsoundconclusionsfromexperimentaldata.

Superior Workflow for High Resolution Scans

TheQuantumGXimagingsystemfeaturesanadvancedworkflowforcreatinghighresolutionimagesfromtheoriginalwholeimagescan.Awholebodyscanisfirsttakenandthenregionsofinterests(ROIs)aredefinedtoreconstructahighresolutionimage.Sincethereisnoneedtorescantheanimal,thethroughputishigherandradiationexposureissignificantlyreduced.ThelargerfieldofviewscanoffersflexibilitytoselectanROIfordetailatalaterstage.SubvolumereconstructionswithvariousresolutionsettingscanbeperformedatanytimeusinganFOVdefinedbytheuser.

Figure 2. A 4.5 micron post reconstruction (bottom image) from initial image (top) which was taken in high resolution mode (FOV 36, 72 micron pixel size).

Day 7 Day 14 Day 21 Day 24

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Figure 5. Two-phase gating technique on the Quantum GX offers reconstructions with less motion artifacts.

FPO

Inspiration

Expiration

Two-Phase Cardiac and Respiratory Gating

ForaccuratemicroCTreconstructionincardiacandrespiratorygatedapplications,itisextremelyimportanttominimizethemotionfromthediaphragmandheart.TheQuantumGX’sadvancedandsimpleintrinsicretrospectivetwo-phasegatingtechniquesareideallysuitedforcardiacandlungfunctionmeasurements.Bysimplydrawingaregionofinterestoverthediaphragmand/orapexoftheheart,theoptionalsoftwarethenreprocessesthedata,usingonlytheviewsfromtheselectedsliceintherespiratoryorcardiaccycle,reducingmotionartifactsin the reconstruction.

Figure 4. Optical co-registration of luciferase labeled MDA-MB-231 metastases with microCT.

Optical Co-registration for Multimodality Imaging

PerkinElmerhasrevolutionizedpreclinical3DopticalimagingwiththeIVISandFMTplatforms.TheQuantumGXmakesmicroCTimagingaseasyasopticalimagingontheIVISandallowsresearcherstoco-registerfunctionalopticalsignalswithanatomicalmicroCTdata.Throughputanduserworkflowhavebeensimplifiedtoseamlesslyco-registeranatomicalandfunctionaldataateverystepofalongitudinalstudy.

Multispecies Imaging

TheQuantumGXfeaturesisatruemultispeciesmicroCTsystemwithaboresizelargeenoughtofitentiremice,ratsandevenrabbits.TheboresizeintheQuantumGXis163mm andwithavailablemouse,ratandrabbitbeds,animalsupto5kgcanbeeasilyimaged.Anentiremousecanbeimagedinonescan.Aratcanbeimagedintwosetsofscansandaguineapigcanbeimagedinthreesetsofscans.

FPO

Mouse

Rat

Figure 6. The Quantum GX is an ideal multispecies imaging system. The system can image entire mice, rats, guinea pigs and even rabbits.

GuineaPig

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Quantum GX High Performance microCT for Preclinical Applications

InjuredNormal

RatwithMIA-inducedosteoarthritisintheknee.Theimageontheleftshowsadiseasedjointcomparedtothenormaljoint on the right

OsteoarthritisNormal

NormalOsteoarthritis

Osteoarthritis Lung Injury

Contrast Agent Enhanced Imaging

Heartandpulmonaryvasculature Livervasculatureimaging 4T1tumorvasculature Lungsegmentation

Additional Applications• Bone research • Bonemorphology(trabecularandcorticalbone)

assessment,BMDmeasurements

• Cardiovascular disease • Myocardialviability,calciumscoring,ventricular

functionandmetabolism,infarcthealing

• Pulmonary disease • Lungairwaystructureandwholelunginacute

injurymodels

•Volume,densityandFRC(functionalresidual capacity)measurements

• Metabolic disease • Differentiationofvisceralandsubcutaneousfat

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Optional Accessories

Other Accessories

•AdvancedmicroCTanalysissoftware

•Cardiac/respiratorygatingsoftware

•3Dmultimodalitymodule

•Adapterarms(Spectrum,FMT)

•Rabbitbed

MouseImagingShuttle

IVISSyringeInjectionSystemXGI-8AnesthesiaSystem

Quantum GX Technical Specifications

CTimage

Fieldofview 72mm(max)

Resolution(pixelsize) 4.5μm(min)

Numberofpixels512×512×512- 8000×8000

X-raytube

Maximumtubevoltage 90kV

Maximumtubecurrent 200 μA

Maximumoutput 8 W

DetectorType Flat panel detector

FrameRate 60fps(max)

CTgantryBoreSize 163mm(max)

Scanablerange 240mm(max)

Scantimes

HighSpeed: 8sec Standard: 18sec,2min HighResolution: 4min, 14min,57min

ImagereconstructionMin.15sec@512×512pixels×512view

SoftwareAcquisition and visualizationpackage

Dimensions(HxWxD) 1450x980x930mm

Weight 450 kg

XWS-260Workbench

For more information, please visit www.perkinelmer.com/invivo

For research use only. Not for use in diagnostic procedures.

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