Applications of the Prominence RF-20Axs Fluorescence Detector ...
Fluorescence of photosensitizer and its diffusion in biological liquid flow Maryakhina V.S.,...
-
Upload
gabriel-carter -
Category
Documents
-
view
215 -
download
0
Transcript of Fluorescence of photosensitizer and its diffusion in biological liquid flow Maryakhina V.S.,...
![Page 1: Fluorescence of photosensitizer and its diffusion in biological liquid flow Maryakhina V.S., Gun’kov v.v. Orenburg state university v.s.maryakhina@gmail.com.](https://reader034.fdocument.org/reader034/viewer/2022051418/5697c01f1a28abf838cd1ead/html5/thumbnails/1.jpg)
Fluorescence of photosensitizer and its diffusion in biological
liquid flow
Maryakhina V.S., Gun’kov v.v.
Orenburg state [email protected]
![Page 2: Fluorescence of photosensitizer and its diffusion in biological liquid flow Maryakhina V.S., Gun’kov v.v. Orenburg state university v.s.maryakhina@gmail.com.](https://reader034.fdocument.org/reader034/viewer/2022051418/5697c01f1a28abf838cd1ead/html5/thumbnails/2.jpg)
![Page 3: Fluorescence of photosensitizer and its diffusion in biological liquid flow Maryakhina V.S., Gun’kov v.v. Orenburg state university v.s.maryakhina@gmail.com.](https://reader034.fdocument.org/reader034/viewer/2022051418/5697c01f1a28abf838cd1ead/html5/thumbnails/3.jpg)
Exemplary fluorescence images of one animal after injection 2 μmol/kg TSC (tetrasulfonated carbocyanine) intravenously into F9-teratocarcinoma
bearing mice
Christin Perlitz, etc //Journal of Fluorescence, Vol. 15, No. 3, May 2005
![Page 4: Fluorescence of photosensitizer and its diffusion in biological liquid flow Maryakhina V.S., Gun’kov v.v. Orenburg state university v.s.maryakhina@gmail.com.](https://reader034.fdocument.org/reader034/viewer/2022051418/5697c01f1a28abf838cd1ead/html5/thumbnails/4.jpg)
EnzymeCells
Monomer photosensitizer
Enzyme
DimerMonomer photosensitizer
fixed
moving
![Page 5: Fluorescence of photosensitizer and its diffusion in biological liquid flow Maryakhina V.S., Gun’kov v.v. Orenburg state university v.s.maryakhina@gmail.com.](https://reader034.fdocument.org/reader034/viewer/2022051418/5697c01f1a28abf838cd1ead/html5/thumbnails/5.jpg)
)(),()(),()(
),()(),(2),(),(
),()(),(2),(),(
1122
222
22
22
111
21
21
tNtrNktNtrNkdt
tdN
trNtNkr
trN
rr
trND
t
trN
trNtNkr
trN
rr
trND
t
trN
)()()()()()(),( 13'
2'
1 21tNStNStNStS
Maryakhina V.S., Gunkov V.V. Computer research and modeling.- 2013.- V.5.- №5.- p. 835-843
Dynamics of bengal rose fluorescence in model solution of collagenase with mammary cells
rr
rrkTk
i
ii
32
![Page 6: Fluorescence of photosensitizer and its diffusion in biological liquid flow Maryakhina V.S., Gun’kov v.v. Orenburg state university v.s.maryakhina@gmail.com.](https://reader034.fdocument.org/reader034/viewer/2022051418/5697c01f1a28abf838cd1ead/html5/thumbnails/6.jpg)
Concentration changes of photosensitizer molecules at immobilization process received from two models
![Page 7: Fluorescence of photosensitizer and its diffusion in biological liquid flow Maryakhina V.S., Gun’kov v.v. Orenburg state university v.s.maryakhina@gmail.com.](https://reader034.fdocument.org/reader034/viewer/2022051418/5697c01f1a28abf838cd1ead/html5/thumbnails/7.jpg)
Changing of fluorescence intensivity at 560 nm
Enzyme
560 nm
545 nm
Ccol=0.1 mg/ml
Ccol=0.5 mg/ml
Maryakhina V.S., Gunkov V.V. Computer research and modeling.- 2013.- V.5.- №5.- p. 835-843
![Page 8: Fluorescence of photosensitizer and its diffusion in biological liquid flow Maryakhina V.S., Gun’kov v.v. Orenburg state university v.s.maryakhina@gmail.com.](https://reader034.fdocument.org/reader034/viewer/2022051418/5697c01f1a28abf838cd1ead/html5/thumbnails/8.jpg)
Comparison of experimental and theoretical results
The normalized experimental (1,2) and theoretical (3,4) fluorescence spectra of erythrosine in collagenase solution 0.1 mkg/ml after addition of dye aliquot with initial concentration: 1,3- 0.1 mМ; 2,4- 1 mМ.
Maryakhina V.S., Gunkov V.V. Computer research and modeling.- 2013.- V.5.- №5.- p. 835-843
![Page 9: Fluorescence of photosensitizer and its diffusion in biological liquid flow Maryakhina V.S., Gun’kov v.v. Orenburg state university v.s.maryakhina@gmail.com.](https://reader034.fdocument.org/reader034/viewer/2022051418/5697c01f1a28abf838cd1ead/html5/thumbnails/9.jpg)
Maryakhina V.S., Letuta S.N.// Laser physics.- 2014.- V. 24.- p. 035601
eosinebengal rose
1- 10-4 М2- 10-3 М
4- health mouse3- cancer 1 cm2- cancer 2 cm1- cancer 3 cm
Fluorescence spectra of dye with collagenase molecule
bengal rose eosine
Phosphorescence of dye with collagenase molecules
![Page 10: Fluorescence of photosensitizer and its diffusion in biological liquid flow Maryakhina V.S., Gun’kov v.v. Orenburg state university v.s.maryakhina@gmail.com.](https://reader034.fdocument.org/reader034/viewer/2022051418/5697c01f1a28abf838cd1ead/html5/thumbnails/10.jpg)
Collagenase (powder) Collagenase (powder) + eosine 10-4М + eosine 10-3М
Fluorescent microscopy results
Maryakhina V.S.// Proc.. of SPIE.- 2013.- V. 8699
![Page 11: Fluorescence of photosensitizer and its diffusion in biological liquid flow Maryakhina V.S., Gun’kov v.v. Orenburg state university v.s.maryakhina@gmail.com.](https://reader034.fdocument.org/reader034/viewer/2022051418/5697c01f1a28abf838cd1ead/html5/thumbnails/11.jpg)
wblood
t1 t2 t3
t1 t2 t3
![Page 12: Fluorescence of photosensitizer and its diffusion in biological liquid flow Maryakhina V.S., Gun’kov v.v. Orenburg state university v.s.maryakhina@gmail.com.](https://reader034.fdocument.org/reader034/viewer/2022051418/5697c01f1a28abf838cd1ead/html5/thumbnails/12.jpg)
For biological liquid flow (blood, for example)
- differential equation of convective diffusion (moving medium)
a few phases
CDgradCwt
C 2
ww, wp, wcell- rate of water, peptide and cell phasesassumptions- ww= wp= wcell
- movable coordinates
),(),(),(),(),(
),(),(),(),(),(
),(),(),(),(),(),(),(
12
2
12
2
212
2
trCtrCkr
trCD
r
trCw
t
trC
trCtrCkr
trCD
r
trCw
t
trC
trCtrCktrCtrCkr
trCD
r
trCw
t
trC
cellwcell
cellcell
cellcell
pwp
pp
pp
cellwpww
ww
ww
),(),(),(),(
),(),(),(),(
),(),(),(),(),(),(
22
2
12
2
212
2
trCtrCkr
trCD
t
trC
trCtrCkr
trCD
t
trC
trCtrCktrCtrCkr
trCD
t
trC
cellwcell
cellcell
pwp
pp
cellwpww
ww
![Page 13: Fluorescence of photosensitizer and its diffusion in biological liquid flow Maryakhina V.S., Gun’kov v.v. Orenburg state university v.s.maryakhina@gmail.com.](https://reader034.fdocument.org/reader034/viewer/2022051418/5697c01f1a28abf838cd1ead/html5/thumbnails/13.jpg)
Initial and bordering conditions
0.5 mm
dvessel = 0.5 cm
Cw(r,0) = Gauss destribution
Dw = 10-9 cm2/s
Dp = 2.8 10-12 cm2/s
Dcell = 4.5 10-15 cm2/s
R
kTDt 6
RR
RRkTk
i
ii
32
Cp(r,0) = 2 Ccell(r,0)
![Page 14: Fluorescence of photosensitizer and its diffusion in biological liquid flow Maryakhina V.S., Gun’kov v.v. Orenburg state university v.s.maryakhina@gmail.com.](https://reader034.fdocument.org/reader034/viewer/2022051418/5697c01f1a28abf838cd1ead/html5/thumbnails/14.jpg)
![Page 15: Fluorescence of photosensitizer and its diffusion in biological liquid flow Maryakhina V.S., Gun’kov v.v. Orenburg state university v.s.maryakhina@gmail.com.](https://reader034.fdocument.org/reader034/viewer/2022051418/5697c01f1a28abf838cd1ead/html5/thumbnails/15.jpg)
0
),()( dr
r
trCtC i
i
600 s
600 s
600 s
Concentration of injected compound
Concentration of free cellsConcentration of free peptides
![Page 16: Fluorescence of photosensitizer and its diffusion in biological liquid flow Maryakhina V.S., Gun’kov v.v. Orenburg state university v.s.maryakhina@gmail.com.](https://reader034.fdocument.org/reader034/viewer/2022051418/5697c01f1a28abf838cd1ead/html5/thumbnails/16.jpg)
Thank you for your attention
This work is partially supported by grant of RFBR №14-01-31081 mol_a