Amino Transferases ( AST , ALT ) Colorimetric Methods
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Amino Transferases ( AST , ALT ) Colorimetric Methods 1 ) Retiman & Frankel Method 2 ) Retiman & Frankal Method equivalent to kinetics 3 ) Kinetic Method ) IFCC , DGKC , SCE , ECCLS ) 4 ) Dry Chemistry
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Amino Transferases ( AST , ALT ) Colorimetric Methods. 1) Retiman & Frankel Method 2) Retiman & Frankal Method equivalent to kinetics 3) Kinetic Method ( IFCC , DGKC , SCE , ECCLS ) 4) Dry Chemistry. Retiman & Frankel Method. L . Aspartate + α Ketoglutatate - PowerPoint PPT Presentation
Transcript of Amino Transferases ( AST , ALT ) Colorimetric Methods
Amino Transferases ( AST , ALT ) Colorimetric Methods
1 )Retiman & Frankel Method 2 )Retiman & Frankal Method equivalent to
kinetics 3 )Kinetic Method ( IFCC , DGKC , SCE , ECCLS )
4 )Dry Chemistry
Retiman & Frankel Method
L . Aspartate + α Ketoglutatate
Oxaloacetic + L . Glutamate
AST
Kinetic Method ( IFCC )
Oxoglutarate + Aspartate Glutamate + Oxaloacetate
Oxaloacetate + NADH + H+ Malate + NAD +
•Decrease in A340nm directly proportional to AST
AST
MDH
Liner
Colorimetric Method Reitman & Frankal
Semi – Log PaperColorimetric Method
Reitman & Frankal
Lab. Results of Transaminases for Different Methods
Dry Chemistry
I.U / L
Kinetic I.U / L
Retiman & Frankal Equivalent to
Kinetic I.U / L
Retiman & Frankal
I.U / L
Test N0 .
44 50 50 22 AST 1
170 144 125 95 AST 2
37 34 35 20 AST 3
29 35 35 17 ALT 4
102 130 90 73 ALT 5
27 40 35 19.7 ALT 6
