Figure S3. D2F2/E2 mammary adenocarcinoma induces α-Her2 immunity endogenously. (A) Experimental scheme. Sera and tumor measurements were collected from BALB/c mice on days 0, 8, 17, 24, and 30 (n= 9). (B) α-Her2 IgG levels were quantified from sera and correlated with tumor volume. Mean D2F2/E2 volume (mm3) and α-Her2 IgG (Ab5 equivalent μg/mL) are plotted on the left and right y-axis respectively. (C) Three mice were euthanized at day 17 to collect spleens for IFN-γ ELISPOT. Splenocytes were isolated and stimulated with either EKB or KB 3T3 lines for 48hr (n=3/group). **P<0.01 Unpaired t-test.

B C

A

Day -13 0 8 17 24 30

D2F2/E2 inoculation Analysis