Supplemental Figure 1

Supplemental Figure 2

A B

Supplemental Figure 3A

B

Supplemental Figure 4

F (p

g/m

L)

300

400

500SK-OV-3EVSA-TA375HeLa

Secr

eted

TN

F

100

200

300 HeLa

1 μM Birinapant No Treatment0

Supplemental Figure 5

Supplemental Figure 6

A

B

Supplemental Figure 7

Supplemental Table 1

Kd (nM)Birinapant AVPI (Smac)

cIAP1 BIR3 <1 2cIAP2 BIR3 36 20XIAP BIR3 45 130ML-IAP BIR <1 900

Kd, dissociation constant; cIAP, cellular inhibitor of apoptosis; XIAP, X-linked inhibitor of apoptosis; ML-IAP, melanoma inhibitor of apoptosis.