Post on 15-Jul-2020
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C. perfringens A type C. perfringens C type
Materials containing seaweed have MIC-values from 500µg/ml-32µg/ml for C. perfringens (quite good for an extract)
• In comparison, the MIC values of EP100 and EP 200 are 5000μg /ml or higher
Ep109 MeOH
Seaweed 1
Seaweed 2
Seaweed 3
Seaweed 4
Seaweed 5
Seaweed 6
Seaweed 7
Seaweed 8
Ep109 MeOH
Seaweed 1
Seaweed 2
Seaweed 3
Seaweed 4
Seaweed 5
Seaweed 6
Seaweed 7
Seaweed 8
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0
0,1
0,2
0,3
0,4
0,5
0,6
0,7
0,8
0,9
1
Rape meal pH5.5
Rape meal 6.5 EP100 pH 5.5 EP100 pH 6.5 EP109 pH 5.5 EP109 pH 6.5
de
lta
OD
60
0 (
19
hrs
-0h
rs)
LC-MS (UPLC-QTOF-MS)
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LC-MS (UPLC-QTOF-MS)
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GLS + bacteria = 20 fold more lactateJane Mullaney 2013 (PhD thesis)
Enterococcus
Pseudomonas
Acinetobacter
Bacteroides
Lactobacillus
Enterobacter
Bacteroides
Enterococcus
Escherichia
Acinetobacter
Enterobacter
Duodenum
Jejunum
Ewers (ctrl) EP100
Duodenum
Jejunum
Bacteroides
Enterococcus
Propionibacterium
Lactobacillus
Bacteroides
Enterococcus
Lactobacillus
Pediococcus
Pseudomonas
Acinetobacter
Relative abundance. 10 most prevalent bacterial genus for each respective feed and in each respective intestinal part (broilers)
0
2
4
6
8
10
12
14
EWERS/EP100i relative abundance - genus
Salmonella
Mycoplasma
Clostridium
0
2
4
6
8
10
12
Ewers/EP100i Lactobacillus- genus
Lactobacillus
Item Control-gilts EP100-gilts
Piglets/total 16.25 15.75
Live born 14.25 14.42*
Weaning (28 d) 11.25 13.17*
BW at birth 1.12 1.17
BW at weaning 5.99 5.57
Litter BW - weaning 67.39 73.36*
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