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C. perfringens A type C. perfringens C type

Materials containing seaweed have MIC-values from 500µg/ml-32µg/ml for C. perfringens (quite good for an extract)

• In comparison, the MIC values of EP100 and EP 200 are 5000μg /ml or higher

Ep109 MeOH

Seaweed 1

Seaweed 2

Seaweed 3

Seaweed 4

Seaweed 5

Seaweed 6

Seaweed 7

Seaweed 8

Ep109 MeOH

Seaweed 1

Seaweed 2

Seaweed 3

Seaweed 4

Seaweed 5

Seaweed 6

Seaweed 7

Seaweed 8

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0

0,1

0,2

0,3

0,4

0,5

0,6

0,7

0,8

0,9

1

Rape meal pH5.5

Rape meal 6.5 EP100 pH 5.5 EP100 pH 6.5 EP109 pH 5.5 EP109 pH 6.5

de

lta

OD

60

0 (

19

hrs

-0h

rs)

LC-MS (UPLC-QTOF-MS)

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LC-MS (UPLC-QTOF-MS)

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GLS + bacteria = 20 fold more lactateJane Mullaney 2013 (PhD thesis)

Enterococcus

Pseudomonas

Acinetobacter

Bacteroides

Lactobacillus

Enterobacter

Bacteroides

Enterococcus

Escherichia

Acinetobacter

Enterobacter

Duodenum

Jejunum

Ewers (ctrl) EP100

Duodenum

Jejunum

Bacteroides

Enterococcus

Propionibacterium

Lactobacillus

Bacteroides

Enterococcus

Lactobacillus

Pediococcus

Pseudomonas

Acinetobacter

Relative abundance. 10 most prevalent bacterial genus for each respective feed and in each respective intestinal part (broilers)

0

2

4

6

8

10

12

14

EWERS/EP100i relative abundance - genus

Salmonella

Mycoplasma

Clostridium

0

2

4

6

8

10

12

Ewers/EP100i Lactobacillus- genus

Lactobacillus

Item Control-gilts EP100-gilts

Piglets/total 16.25 15.75

Live born 14.25 14.42*

Weaning (28 d) 11.25 13.17*

BW at birth 1.12 1.17

BW at weaning 5.99 5.57

Litter BW - weaning 67.39 73.36*

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