anti-UCH L1

anti-α-tubulin

H157

H358

SW40

3

COLO205

KM12

14

CaCO2

RIE-1

SMC

HUVEC

MCF7

Primary cells

Lung cancer cells

Small intestine epithelial cells

Colon cancer cells

Breast cancer cells

Supplementary Data (S 1.)

5h

71h

immediately

7h

b

a

control UCH-L1 RNAiuninfected

control UCH-L1 RNAiuninfected

Supplementary Data (S 2.)

anti UCH-L1

anti -tubulin

control UCH-L1 RNAi

wc sol. insol. wc sol. insol. wc sol. insol.

uninfected

control UCH-L1-myc

wc sol. insol. wc sol. insol. wc sol. insol.

uninfected

anti UCH-L1

anti -tubulin

UCH-L1-mycEndo UCH-L1

3 g 6 g

wc sol. insol. wc sol. insol. wc sol. insol.

Empty vector

anti UCH-L1

anti -tubulin

Flag-UCH-L1

Endo UCH-L1

Flag-UCH-L1

Supplementary Data (S 3.)

b

a

c

Supplementary Data (S 4.)

M1M2

M3

M1M2

M3

M1M2

M3

M1M2

M3

control UCH-L1 RNAicontrol UCH-L1 RNAi

control UCH-L1 RNAi

phase control UCH-L1 RNAi

G1 (M1) 46.59 47.69

S (M2) 22.18 20.60

G2/M (M3) 30.58 31.48

phase control UCH-L1 RNAi

G1 (M1) 54.73 56.30

S (M2) 17.04 16.15

G2/M (M3) 27.41 26.89

b

a