anti-UCH L1
4
anti-UCH L1 anti-α-tubulin H157 H358 SW403 COLO205 KM1214 CaCO2 RIE-1 SMC HUVEC MCF7 Primary cells Lung cancer cells Small intestine epithelial cells Colon cancer cells Breast cancer cells Supplementary Data (S 1.)
description
Supplementary Data (S 1.). Small intestine epithelial cells. Lung cancer cells. Breast cancer cells. Colon cancer cells. Primary cells. COLO205. H157. MCF7. HUVEC. KM1214. SW403. CaCO2. RIE-1. H358. SMC. anti-UCH L1. anti-α- tubulin. Supplementary Data (S 2.). a. immediately. - PowerPoint PPT Presentation
Transcript of anti-UCH L1
anti-UCH L1
anti-α-tubulin
H157
H358
SW40
3
COLO205
KM12
14
CaCO2
RIE-1
SMC
HUVEC
MCF7
Primary cells
Lung cancer cells
Small intestine epithelial cells
Colon cancer cells
Breast cancer cells
Supplementary Data (S 1.)
5h
71h
immediately
7h
b
a
control UCH-L1 RNAiuninfected
control UCH-L1 RNAiuninfected
Supplementary Data (S 2.)
anti UCH-L1
anti -tubulin
control UCH-L1 RNAi
wc sol. insol. wc sol. insol. wc sol. insol.
uninfected
control UCH-L1-myc
wc sol. insol. wc sol. insol. wc sol. insol.
uninfected
anti UCH-L1
anti -tubulin
UCH-L1-mycEndo UCH-L1
3 g 6 g
wc sol. insol. wc sol. insol. wc sol. insol.
Empty vector
anti UCH-L1
anti -tubulin
Flag-UCH-L1
Endo UCH-L1
Flag-UCH-L1
Supplementary Data (S 3.)
b
a
c
Supplementary Data (S 4.)
M1M2
M3
M1M2
M3
M1M2
M3
M1M2
M3
control UCH-L1 RNAicontrol UCH-L1 RNAi
control UCH-L1 RNAi
phase control UCH-L1 RNAi
G1 (M1) 46.59 47.69
S (M2) 22.18 20.60
G2/M (M3) 30.58 31.48
phase control UCH-L1 RNAi
G1 (M1) 54.73 56.30
S (M2) 17.04 16.15
G2/M (M3) 27.41 26.89
b
a
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