Post on 14-Jan-2016
description
5 μM
15 μM
30 μM
60 μM
100 μM150 μM200 μM
Linoleic acid
Reaction Time (min)10 20 30
Reaction time (min)
5 10 15 20 25 30
B
Lipo
xyge
nase
act
ivity
(n
kat m
g-1)
A
C
0
20
40
60
80
100
120
140
4 5 6 7 8 9
Linoleic acid
0
50
100
150
200Linoleic acid Arachidonic acid
0
100
150
200
250
50
Lipo
xyge
nase
act
ivity
(n
kat m
g-1)
Lipo
xyge
nase
act
ivity
(n
kat m
g-1)
pH
Supplemental Figure S3. LOX activity of recombinant His-tag-CaLOX1 expressed in E. coli BL21 (DE3). A, Effect of pH on CaLOX1 LOX activity. Purified CaLOX1 was analyzed for enzyme activity in phosphate buffers with different pH values using 0.1 mM linoleic acid as a substrate. B, Time courses of CaLOX1 LOX activity. Purified CaLOX1 was incubated with 0.1 mM of linoleic acid or arachidonic acid as a substrate. C, Time courses of the LOX activity of purified CaLOX1, as determined with various concentrations of linoleic acid as a substrate.