Figure S1

LC3

SQSTM1

ACTB

con 0.5 μM 1 μM

HCT116 cells

SAHA

HepG2 cells

con 1 μM 3 μM

SAHA

LC3

SQSTM1

ACTB

con 0.5 μM 1 μM

HCT116 cells

SAHA

FOXO1

CDKN1A

ACTB

HepG2 cells

con 1 μM 3 μM

SAHA

FOXO1

CDKN1A

ACTB

Figure S2

A

HCT116 cells

con 0.125 0.25 μM

TSA

FOXO3

ACTB

FOXO4

HepG2 cells

con 0.25 0.5 μM

TSA

FOXO3

ACTB

FOXO4

B

HCT116 cells HepG2 cells

TSA - 0.125 0.25 μM - 0.125 0.25 μM - 0.25 0.5 μM - 0.25 0.5 μM

FOXO3 FOXO4 FOXO3 FOXO4

Figure S3

con 0.125 0.25 0.5 μM

FOXO1

TP53

CDKN1A

ACTB

TSA

con 0.125 0.25 0.5 μM

TSA

TP53+/+ HCT116 cell TP53-/- HCT116 cell

Figure S4

SQSTM1

BAF - - - - + + + +

HepG2 cells

FOXO1

LC3

ACTB

si-con si-FOXO1 si-con si-FOXO1

TSA - + - + - + - +

Figure S5

FOXO3

LC3

SQSTM1

ACTB

si-con si-FOXO3

TSA - + - +

FOXO4

LC3

SQSTM1

ACTB

si-con si-FOXO4

TSA - + - +

Figure S6

B

A

ATG4B ATG12

PIK3C3 BECN1

Figure S7

FOXO1

p-RPS6

si-con si-FOXO1

HepG2 cells

TSA - + - +

RPS6

p-EIF4EBP1

EIF4EBP1

ACTB

Figure S8

HCT116 cells

SAHA - + - + - + CQ BAF

Figure S9

A

C TSA - + - +

si-con si-ATG7

ATG7

Cleaved CASP3

ACTB

Cleaved PARP1

control TSA

si-con

si-ATG7

200 μm

B

TSA - + - +

Figure S10

B TSA - + - +

si-con si-FOXO1

FOXO1

Cleaved CASP3

ACTB

Cleaved PARP1

si-con si-FOXO1

control TSA control TSA

A

200 μm

Figure S11