5 μM
1
5 μM 15 μM 30 μM 60 μM 100 μM 150 μM 200 μM Linoleic acid Reaction Time (min) 10 20 30 Reaction time (min) 5 10 15 20 25 30 B Lipoxygenase activity (nkat mg -1 ) A C 0 20 40 60 80 100 120 140 4 5 6 7 8 9 Linoleic acid 0 50 100 150 200 Linoleic acid Arachidonic acid 0 100 150 200 250 50 Lipoxygenase activity (nkat mg -1 ) Lipoxygenase activity (nkat mg -1 ) pH Supplemental Figure S3. LOX activity of recombinant His-tag-CaLOX1 expressed in E. coli BL21 (DE3). A, Effect of pH on CaLOX1 LOX activity. Purified CaLOX1 was analyzed for enzyme activity in phosphate buffers with different pH values using 0.1 mM linoleic acid as a substrate. B, Time courses of CaLOX1 LOX activity. Purified CaLOX1 was incubated with 0.1 mM of linoleic acid or arachidonic acid as a substrate. C, Time courses of the LOX activity of purified CaLOX1, as determined with various concentrations of linoleic acid as a substrate.
description
A. B. 200. 140. Linoleic acid. Linoleic acid. Arachidonic acid. 120. 150. 100. 80. Lipoxygenase activity (nkat mg -1 ). Lipoxygenase activity (nkat mg -1 ). 100. 60. 40. 50. 20. 0. 0. 4. 5. 6. 7. 8. 9. 10. 20. 30. pH. Reaction Time (min). C. 250. - PowerPoint PPT Presentation
Transcript of 5 μM
5 μM
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Linoleic acid
Reaction Time (min)10 20 30
Reaction time (min)
5 10 15 20 25 30
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Supplemental Figure S3. LOX activity of recombinant His-tag-CaLOX1 expressed in E. coli BL21 (DE3). A, Effect of pH on CaLOX1 LOX activity. Purified CaLOX1 was analyzed for enzyme activity in phosphate buffers with different pH values using 0.1 mM linoleic acid as a substrate. B, Time courses of CaLOX1 LOX activity. Purified CaLOX1 was incubated with 0.1 mM of linoleic acid or arachidonic acid as a substrate. C, Time courses of the LOX activity of purified CaLOX1, as determined with various concentrations of linoleic acid as a substrate.
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