Fig. S1. Carotenoid and chlorophyll composition of Arabidopsis Z-ISO mutants compared to wild type. Seedlings were grown for 6d on half strength MS plus 1% sucrose in the light at 250 C. w.t.(Col), zic1-1 and zic1-2. N, (neoxanthin); V, (violaxanthin); L, (lutein); Cb (Chlorophyll b); Ca (Chlorophyll a), β, (β-carotene) were marked. HPLC chromatogram is shown at 450 nm.

zic1-1

N V

LCb Ca

β

Fig. S2A. Alignment of Z-ISO amino acid sequences from Zea mays (Z-ISO_Zm), Sorghum bicolor (Z-ISO_Sm), Oryza sativa japonica (Z-ISO_Os), Phyllostachys edulis (Z-ISO_Pe), Brachypodium distachyon (Z-ISO_Bd), Solanum lycopersicum (Z-ISO_Sl), Ricinus communis (Z-ISO_Rc), Populus trichocarpa (Z-ISO_Pt), Vitis vinifera (Z-ISO_Vv), Medicago truncatula (Z-ISO_Mt), Glycine max (Z-ISO_Gm), Arabidopsis thaliana (Z-ISO_At), Picea sitchensis (Z-ISO_Ps), Volvox carteri (Z-ISO_Vc), Physcomi trelapatens (Z-ISO_Pt), Thalassiosira pseudonana (Z-ISO_Tp), Prochlorococcus marinus str. MIT9211 (Z-ISO_Pm), Synechococcus elongatus PCC 6301(Z-ISO_Se). Alignment was performed by ClustalW using MEGA3.1. Protein sequences were obtained from NCBI (www.ncbi.nlm.nih.gov) and Genbank accession numbers are listed in Table S2.

Fig. S2A. , continued

Fig. S2B. Alignment of Z-ISO amino acid sequences from Zea mays (Z-ISO_Zm), Oryza sativa japonica (Z-ISO_Os), Solanum lycopersicum (Z-ISO_Sl), Medicago truncatula (Z-ISO_Mt), Arabidopsis thaliana (Z-ISO_At), Picea sitchensis (Z-ISO_Ps), Thalassiosira pseudonana (Z-ISO_Tp), Synechococcus elongatus PCC 6301(Z-ISO_Se) , and NnrU from Sinorhizobium meliloti 1021 (NnrU_Sm), Hyphomonas neptunium ATCC 15444 (NnrU_Hn); Rhodobacter sphaeroides ATCC 17029 (NnrU_Rs), Rhodopseudomonas palustris HaA2 (NnrU_Rp). Alignment was performed by ClustalW using MEGA3.1. Protein sequences were obtained from NCBI (www.ncbi.nlm.nih.gov) and Genbank accession numbers are listed in Table S2. Abbreviations in the consensus sequences shown at the bottom are as in Figure S2A. Predicted transmembrane domains for selected species (4) are marked with boxes.

Fig. S2B., continued