Supplementary Data

β-actin

IL-13Rα2 65 kDa

42 kDa

G26

-H2

G26

-V2

SnB

19-p

cDN

A

SnB

19-asI

L-13

Rα2

Figure S1A

1012 109 1010 1011 1012 1011 1010 10121011 109 1012 1010 109 1011 1010

Phage clones (pfu/ml)

109

Pep-1 Pep-2 Pep-3 M13KE

Figure S1B

0.0

0.2

0.4

0.6

0.8

DTT (-)

DTT (+)

O.D

. - A

405 n

m

Concentration (µg/ml)

Figure S1C

0.5 1.

02.

5 50.

5 12.

5 50.

5 12.

5 50.

5 12.

5 50.

5 12.

5 5

0.0

0.2

0.4

0.6

0.8

IL-13R2-FcIgG-FcBSA

Pep-1-L Pep-1-DSC Pep-1-L(C/S)

ControlPep-1-L

ControlPep-1-DSC

O.D

. -

A40

5 n

m

1 µg/ml 5 µg/ml

U-251 MG

Pep-1-DSC

Pep-1-L

Figure S2

A

Pep-1-L

B

T98G cells

Pep-1-DSC

Figure S2

1 µg/ml 5 µg/ml

Scrambled Pep-1-L

U-251 MG cells

C

No peptide

1 µg/ml

Figure S2

5 µg/ml

Figure S2

D

Figure S3A

Strep-Cys5.5

Strep-Cys5.5

Strep-Cys5.5

30 min 2 hr 8 hr

24 hr 72 hr

Pep-1-DSCCtrl.

Pep-1-DSC Saline Pep-1-DSCCtrl.

Pep-1-DSC Saline Pep-1-DSCCtrl.

Pep-1-DSC Saline

48 hr

Pep-1

-DSC

Pep-1

-DSC

Ctrl.

0

2

4

6

8

p = 0.0148*

To

tal N

IR R

adia

nt

effi

cien

cy

(x 1

0 )

10

2

24

48

120

144

288

Luciferin

Figure S3BPep-1-DSC Pep-1-DSCCtrl.

Strep-Cy5.5

Saline

hr

Figure S4

A

B

i. ii. iii.

i. ii. iii.