Selection for high β-glucan content and good agronomic performance in oat grain

Selection for high -glucan content and good agronomic performance in oat grainAlona A. ChernyshovaProfessor: Jean-Luc JanninkIowa State UniversityDepartment of Agronomy

American Oat Workers ConferenceFargo, ND25 July 2006

OutlineIntroductionObjectivesMaterials and methodsResultsConclusions

-glucan(1-3)(1-4)--D is a soluble fiber found in the endosperm and subaleurone layers of cereal seeds

Introduction

IntroductionThe structure of oat groat -glucan(1-3)(1-4)--D is a soluble fiber found in the endosperm and subaleurone layers of cereal seeds-glucan is responsible for many health related effects of oat:reduction of blood cholesterol levelsreduction of the risk of coronary heart diseasereduction of the risk of diabetesattenuation of blood glucose and insulin, and prolonged satiety Standard oat varieties contain from 4.5 to 5.0 % of -glucanWe would like to double this concentration

IntroductionViscosity is the thickness or resistance to flow of a liquidMany health related effects of oats result from the high viscosity of their soluble -glucansRegression analyses have shown a positive effect of -glucan content on viscosity Viscosity deviationDeviation of viscosity from its prediction based on -glucan contentWe call this the viscosity deviation Viscosity of some oat lines deviated significantly from the regression prediction, indicating that, for a given -glucan content, some lines generate more viscosity and some less viscosity

Objectivesto estimate the genetic components of variance in -glucan content, viscosity, and viscosity deviation in high -glucan lines, elite agronomic lines, and in their population crosses

to evaluate the differences between elite agronomic lines and high -glucan lines for -glucan, viscosity, and viscosity deviation

to use a powerful population design to detect epistatic interaction among parents

Materials and methodsFigure 2. Experimental program

Materials and methodsNorth Carolina Design IIMating design

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The -glucan concentration in flours was determined enzymatically using a mixed linkage -glucan kit (Megazyme Int., Wicklow, Ireland)

Modifications were made to the Approved Method, which allowed us to increase the number of samples analyzed per unit time and cost spentMaterials and methodsMicro Enzymatic Method

Materials and methodsMicro Enzymatic Method

Materials and methods Microplate reader measures the light absorbance in each cell that reflects the amount of -glucan in the sample Micro Enzymatic Method

Materials and methodsGlucose standards

Chart1

0.450.0404

0.62756

Glucose standards, g/mL

Optical density

SAS

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EDIT

BLOCK 5 + BLOCK 610/10/05BLOCK 7 + BLOCK 8BLOCK 7 + BLOCK 8OD ResultsBLOCK 2+3+ 9BLOCK 2+BLOCK 3BLOCK 9BLOCK 2BLOCK 10+1110/24/05BLOCK 10+1110/24/05

0.1460.0472HBLOCK 5+ BLOCK 6116A0.3740.3331B526B0.389219C0.365BLOCK 10 + BLOCK 11WEIGHTBLOCK 14block 18

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1C0.420.4181D0.2540.0475H314A0.3950.384422E0.430.4111E1729F0.2670.3691D521F0.3851529F0.3920.4031D129F100.290.3583199.720.47351100.510.446

1D0.4150.431E0.3640.0476H328B0.4670.4841325C0.340.2831F18211A0.3310.3251E8112B0.3372511C0.3280.0471E319C100.260.40232100.120.5065299.70.403

1E0.4260.4091F0.3560.0467H5111C0.3060.3081316C0.3210.3021G1811C0.3320.3851F8210E0.3425211A0.3320.341F326B100.650.429BLOCK 13131100.630.2987199.640.364

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1G0.330.3292A0.4390.0469H614E0.4380.4261414F0.320.3162B1916F0.2880.3842A1724C0.3344923E0.3960.0492A13211A100.120.2431024B100.560.437141100.120.3478199.850.449

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4B0.3370.3524F1922C0.2520.25210729E0.3710.344G227112F0.340.3774F209111G0.3132424A0.2570.3394F2324B99.930.3072027E100.680.3058417D100.230.34134913A99.620.343932100.660.3841982100.230.383

4C0.3420.3224G2017A0.2690.279107111E0.3580.2955A22725G0.3430.3384G20923G0.33299110C0.3330.3434G2419A99.740.24515617F100.060.33684212D100.410.34834924A100.570.35101199.480.3432061100.460.403

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10D0.3790.37311G13G0.0470.04729D0.0470.35212B0.3686H0.31311G0.28111G0.2624H33426G100.030.351237211B99.960.412

10E0.3210.31812B17C0.2990.29914E0.0480.30312C0.3577H0.33712B0.33112B0.2685H34215E99.880.40724514E99.860.316

10F0.3380.36712C111A0.0480.047212E0.0470.36912D0.4678H0.34212C0.31812C0.3726H34224D100.430.39324523F100.610.329

10G0.2990.28512D25C0.0470.04612F0.0460.04712E0.4819H0.37812D0.36212D0.377H35013C99.560.42525318F100.330.2610.1622H

10H0.3380.37612E26E0.0460.04823F0.0480.3412F0.54310H0.31712E0.04712E0.4788H35025B100.60.395253212E99.70.2510.1733H

11A0.3460.34212F29C0.0470.04718F0.0470.40812G0.56411H0.29812F0.38712F0.4859H35817D100.340.349261110A99.970.2910.2884H

11B0.2710.26912G211E0.0460.0470.31412G0.25212G0.57810H358212D100.160.30526125D99.980.3430.2895H

11C0.5911H366112F100.260.30226912F100.630.3420.3926H

11D36625G100.680.33826929D100.470.3350.3957H

11E37418C100.110.24427716A100.380.3220.5338H

11F37422B99.440.22427724B100.020.3410.539H

11G382112C100.250.3050.62510H

11H38221B100.670.3340.62511H

12A39018E99.210.284

12B39027G100.630.344

12C39819G99.710.298

12D39824G100.740.288

12E111G0.166

12F23G0.045

12G

12H

0.1482H

0.1553H

0.2554H

0.265H

0.3556H

0.3627H

0.4628H

0.4759H

0.56810H

0.56411H

BLOCK 5+ BLOCK 6

5111C

529G

614E

626B

712F

724F

8110E

826A

1716D

1721G

1815D

1822D

1919D

1922C

2017A

2027G

15413A

15427E

17018G

170212F

17818D

17828A

194110A

19427D

202110G

202211B

218110F

21821B

24219E

24222B

25014D

250211F

258110B

25821D

27419B

27429A

114111G

11424C

7417B

74212E

5018F

5024B

111C

123D

16112D

1622G

12216G

12226C

14613B

14625B

13813C

13824G

1518C

15211D

5819F

5821F

26110D

2623E

1413F

1426F

314A

328B

34110C

34212C

4212A

4225A

13015F

13028E

98112B

9825E

9015G

9021E

1317F

13212G

BLOCK 15

519A100.030.373

522E100.350.414

716C100.80.392

725C99.580.413

814F100.350.436

825A100.220.389

1716E100.440.27

17210D100.030.283

1811G100.70.314

18211D100.240.283

19110B100.560.242

19210F100.450.227

2012C100.730.238

BLOCK 162025F100.20.239

1019C100.530.384157111C100.40.263

1026B1000.403157212G100.520.264

2111D100.030.25417317B99.570.357

2129F100.290.27317323B100.020.365

2211C100.350.21181110G100.620.352

22211A100.470.22218122D100.660.284

2316F99.90.27518913A100.680.304

2323E100.570.28718924A99.820.329

2413D100.460.272197110C100.40.3

2429B100.140.26419721E99.770.31

28618D100.310.353205111E100.120.329

28627E100.040.38920529E99.970.267

29417F101.860.23921318G100.110.404

29421F99.60.24921327A100.450.43

302112B100.360.38122112A100.950.256

302210E99.560.40822122G100.590.303

32617C100.840.259229111F100.540.274

32624C100.480.26422926D99.590.25

33418A100.160.32323718B100.290.371

33426G100.030.351237211B99.960.412

34215E99.880.40724514E99.860.316

34224D100.430.39324523F100.610.329

35013C99.560.42525318F100.330.261

35025B100.60.395253212E99.70.251

35817D100.340.349261110A99.970.291

358212D100.160.30526125D99.980.343

366112F100.260.30226912F100.630.342

36625G100.680.33826929D100.470.335

37418C100.110.24427716A100.380.322

37422B99.440.22427724B100.020.341

382112C100.250.305

38221B100.670.334

39018E99.210.284

39027G100.630.344

39819G99.710.298

39824G100.740.288

111G0.166

23G0.045

0.1482H

0.1553H

0.2554H

0.265H

0.3556H

0.3627H

0.4628H

0.4759H

0.56810H

0.56411H

BLOCK 17WEIGHT

119C100.430.414

126B100.750.4090.1552H

211D99.810.3770.1573H

229F100.180.4030.2634H

311C100.380.4740.2655H

3211A99.980.4530.3686H

416F100.60.4750.3717H

423E100.240.450.4858H

1313D100.260.2840.4919H

1329B99.910.290.58310H

1418D99.750.3330.59211H

1427E99.990.32

1517F100.40.291

1521F100.050.29

16112B99.470.276

16210E99.960.252

3017C100.270.287

3024C99.860.288

3818A99.790.323

3826G100.140.319

4615E100.350.316

4624D100.430.312

5413C99.60.353

5425B100.660.333

6217D99.860.349

62212D100.340.365

70112F99.950.334

7025G99.530.331

7818C100.120.454

7822B99.360.402

86112C99.590.334

8621B100.480.351

9418E99.960.347

9427G100.360.357

10219G100.550.391

10224G100.290.363

110111G99.40.364

11023G1000.384

11816A100.380.474

11824B100.420.476

12619A99.750.247

12622E99.70.294

13416C100.20.316

13425C100.480.305

14214F100.680.318

14225A99.860.316

15016E99.970.334

150210D100.120.331

CORRELATION

0.3611C10C110.3517F10*F7100.838010*0.7820.004

0.3596B11B60.3513C11*C3110.8280.00711*0.7840.009

0.252D21D20.24411E21*E11210.5620.00521*0.5150

0.3165G22G50.282E22*E2220.6860.00122*0.60

0.3058F23F80.2946D23*D6230.666023*0.6540.002

0.22811G24G110.2617B24*B7240.481024*0.5330.004

0.3654G281G40.32611B281*B112810.7340.009281*0.6860

0.429C289C90.3598D289*D82890.8270.008289*0.7780.008

0.3279G297G90.3096E297*E62970.7480297*0.690.006

0.2742F321F20.2779F321*F93210.5860.007321*0.5870.004

0.2933F329F30.2644B329*B43290.6380.005329*0.5310.006

0.3285C345C50.3157E345*E73450.6570345*0.7020

0.33811D353D110.32810D353*D103530.7470.002353*0.6920.001

0.3477G361G70.4022B361*B23610.7230361*0.918

0.3118B369B80.2969E369*E93690.6770369*0.6250.005

0.33410C377C100.332G377*G23770.7230.001377*0.742

spikestandard

*********averagestdev*********averageCORRELATION FOR AVERAGE VALUES

100.360.3510.35550.0063639610.8380.7820.810.03959797970.9697321588

110.3590.3510.3550.00565685420.8280.7840.8060.0311126984

210.250.2440.2470.00424264070.5620.5150.53850.0332340187CORRELATION FOR REP 1

220.3160.280.2980.02545584410.6860.60.6430.06081118320.9270542338

230.3050.2940.29950.00777817460.6660.6540.660.0084852814

240.2280.2610.24450.02333452380.4810.5330.5070.0367695526CORRELATION FOR REP 2

2810.3650.3260.34550.02757716450.7340.6860.710.03394112550.9873471479

2890.420.3590.38950.04313351370.8270.7780.80250.0346482323

2970.3270.3090.3180.01272792210.7480.690.7190.0410121933

3210.2740.2770.27550.00212132030.5860.5870.58650.00070710680.41975183410.0291451487

3290.2930.2640.27850.02050609670.6380.5310.58450.07566042560.02824691980.0195515367

3450.3280.3150.32150.00919238820.6570.7020.67950.03181980520.94038045990.010725566

3530.3380.3280.3330.00707106780.7470.6920.71950.038890873220.822341357214

3610.3470.4020.37450.0388908730.7230.9180.82050.13788582230.02540290880.0016105287

3690.3110.2960.30350.01060660170.6770.6250.6510.0367695526

3770.3340.330.3320.00282842710.7230.7420.73250.0134350288

0.360.8380.9516322685

0.3590.828

0.250.562

0.3160.686

0.3050.666

12/31/990.481

0.3650.734

0.420.827

0.3270.748

0.2740.586

0.2930.638

0.3280.657

0.3380.747

0.3470.723

0.3110.677

0.3340.723

0.3510.782

0.3510.784

0.2440.515

0.280.6

0.2940.654

0.2610.533

0.3260.686

0.3590.778

0.3090.69

0.2770.587

0.2640.531

0.3150.702

0.3280.692

0.4020.918

0.2960.625

0.330.742

PLATE READER

BLOCK 19/26/05BLOCK 29/28/05BLOCK 310/4/05BLOCK 410/5/05BLOCK 5 + BLOCK 610/10/05BLOCK 7 + BLOCK 810/12/05BLOCK 2+3+910/17/05BLOCK 10+1110/24/05BLOCK 12 + 1310/25/05BLOCK 16 + BLOCK 1511/1/05BLOCK 1711/4/05block 14+1812/19/05block 19 + block 2012/21/05block 21+221/3/06block 23+241/5/06replication of samples 17, 20, 225, 2551/30/06redo samples2/6/06

0.0421A0.0410.040.0580.040.0460.0461A0.0471A0.0471A0.0411A0.0411A0.0441A0.0440.4111A0.0511A0.0531A0.0531A0.0441A17 and 20 correspond to block 231A0.472

0.041B0.0420.0390.0770.1480.3670.3681B0.3331B0.3421B0.2941B0.4691B0.3341B0.3510.3721B0.4521B0.0511B0.2491B0.0411B225, 255 correspond to block 211B0.392

0.0411C0.040.0390.090.1520.4670.4461C0.3321C0.3281C0.3141C0.2961C0.211C0.4740.2981C0.4451C0.4061C0.3961C0.0411C1C0.407

0.0421D0.0410.0410.0830.2570.420.4181D0.2781D0.3691D0.4031D0.3991D0.2541D0.3770.4731D0.3941D0.4091D0.3621D0.0451D1D0.405

0.041E0.0410.040.0880.2610.4150.431E0.4111E0.3251E0.0471E0.3281E0.311E0.0440.3831E0.3281E0.3571E0.3891E0.0411E1E0.41

0.0391F0.0410.0410.0830.3720.4260.4091F0.2831F0.3851F0.341F0.2991F0.2491F0.290.3531F0.3071F0.2961F0.0521F0.041F1F0.327

0.0411G0.0410.0410.0590.2620.2970.2941G0.3021G0.3251G0.3321G0.2891G0.3141G0.0460.3081G0.1971G0.2671G0.341G0.0411G1G0.408

0.0411H0.0410.040.0450.0410.0460.0471H0.0471H0.0491H0.041H0.041H0.0441H0.0480.0411H0.0511H0.0511H0.0521H0.0411H1H0.054

0.0422A0.0430.0390.0690.0390.330.3292A0.3362A0.3842A0.0492A0.2932A0.2562A0.0440.2812A0.3362A0.3112A0.432A0.0432A2A0.357

0.4022B0.1420.0430.1250.0440.160.32B0.3162B0.3832B0.3622B0.3462B0.2242B0.4020.4492B0.4342B0.0492B0.3412B0.2812B2B0.452

0.0442C0.2620.0440.0970.340.2520.2522C0.3962C0.3762C0.3772C0.3872C0.2382C0.0440.292C0.2612C0.3422C0.4072C0.0552C2C0.322

0.252D0.3440.0430.1040.3740.3110.3222D0.4532D0.3292D0.3212D0.0482D0.2842D0.0440.2792D0.2812D0.052D0.4472D0.2852D2D0.424

0.282E0.0430.0440.2180.3240.0460.0462E0.432E0.3382E0.262E0.4372E0.4142E0.2940.4032E0.3462E0.4072E0.3772E0.0512E2E0.448

0.2742F0.0430.0450.0980.3970.3560.3492F0.0462F0.0472F0.0482F0.0492F0.3422F0.0440.0552F0.3082F0.0512F0.6212F0.3022F2F0.054

0.332G0.1440.0430.0890.3770.2870.2882G0.3382G0.3872G0.0472G0.3362G0.3032G0.0450.2862G0.3062G0.3282G0.4532G0.0512G2G0.381

0.0412H0.040.0410.0590.1550.1460.0472H0.1522H0.1572H0.1572H0.1562H0.1482H0.1550.1622H0.162H0.1592H0.1642H0.1572H2H0.155

0.0443A0.040.0390.0770.0430.340.3373A0.3593A0.2683A0.2923A0.3433A0.3043A0.0460.393A0.4233A0.3143A0.3153A0.2883A3A0.559

0.0553B0.0450.0450.1380.0460.3240.3233B0.3163B0.2493B0.0483B0.3193B0.3653B0.0450.3543B0.2513B0.2883B0.4373B0.0533B3B0.386

0.3513C0.3670.0490.1010.3860.3010.2993C0.3343C0.3123C0.053C0.3853C0.4253C0.3530.4433C0.4023C0.3193C0.3023C0.3143C3C0.36

0.0513D0.0440.0450.2150.0490.4510.4753D0.2893D0.4533D0.2623D0.3053D0.2723D0.2840.3453D0.3133D0.3023D0.2643D0.0533D3D0.431

0.0463E0.3330.0450.1170.2670.380.3753E0.2833E0.3963E0.3173E0.3273E0.2873E0.450.3443E0.4573E0.2793E0.0533E0.3183E3E0.396

0.2933F0.3960.0450.0990.0480.3070.2933F0.0483F0.283F0.0493F0.0513F0.3293F0.0460.3023F0.3713F0.3223F0.313F0.0553F3F0.056

0.0463G0.0440.0440.0890.0470.0470.0473G0.2593G0.333G0.2833G0.3253G0.0453G0.3840.3613G0.3923G0.3183G0.4933G0.3193G3G0.323

0.0433H0.040.040.0570.1580.1540.0493H0.1563H0.163H0.1613H0.1593H0.1553H0.1570.1733H0.1643H0.1643H0.1663H0.1623H3H0.167

0.0414A0.040.0380.0950.0380.3950.3844A0.3544A0.2574A0.3354A0.354A0.3294A0.0440.3834A0.4314A0.3134A0.3044A0.044A4A0.275

0.2644B0.3460.0460.1180.0450.3540.3474B0.4134B0.3714B0.3074B0.4374B0.3414B0.4760.2694B0.4584B0.3634B0.4334B0.2784B4B0.36

0.0454C0.3780.0430.1170.240.4090.4044C0.3264C0.3344C0.4484C0.2974C0.2644C0.2880.4224C0.3344C0.4014C0.3554C0.0534C4C0.312

0.0454D0.280.0440.110.3410.4390.4284D0.374D0.2754D0.334D0.3844D0.3934D0.3120.424D0.3514D0.3634D0.3434D0.2884D4D0.39

0.0444E0.3360.0430.1050.0450.4380.4264E0.0484E0.2864E0.0484E0.0494E0.3164E0.0440.3154E0.3414E0.3154E0.3144E0.0534E4E0.05

0.0454F0.2920.0460.1140.3360.3370.3524F0.324F0.3774F0.3394F0.2454F0.4364F0.3180.4494F0.4374F0.2914F0.3354F0.34F4F0.355

0.3654G0.0440.0440.0930.0440.3420.3224G0.344G0.3384G0.3434G0.3664G0.2884G0.3630.3174G0.3634G0.274G0.2984G0.0534G4G0.364

0.0424H0.0420.0410.0660.270.2620.0464H0.2574H0.2594H0.2674H0.2624H0.2554H0.2630.2884H0.2714H0.2754H0.2784H0.2624H4H0.269

0.045A0.0380.0380.1070.0380.3490.355A0.2955A0.3865A0.3525A0.2485A0.3895A0.3160.3445A0.4985A0.2765A0.3425A0.045A5A0.301

0.0455B0.4740.0460.4390.0450.3150.3125B0.3185B0.3045B0.0495B0.3865B0.3955B0.3330.4745B0.4045B0.315B0.3375B0.0485B5B0.056

0.3285C0.0440.0450.4290.3450.0470.0465C0.345C0.3525C0.3785C0.2735C0.4135C0.3050.4055C0.45C0.3395C0.4675C0.0425C5C0.33

0.0445D0.3150.0460.1130.0450.3020.3155D0.0475D0.3285D0.0485D0.055D0.3435D0.0450.3415D0.3265D0.3425D0.3455D0.0435D5D0.054

0.0455E0.2950.0460.5420.0450.360.365E0.3755E0.2675E0.3555E0.3495E0.4075E0.3160.4225E0.3895E0.395E0.5165E0.0425E5E0.055

0.0425F0.3010.0430.110.0430.3460.3565F0.4125F0.3925F0.3645F0.4265F0.2395F0.0440.2945F0.2885F0.3275F0.3815F0.0425F5F0.416

0.3165G0.3150.0470.0950.0460.4090.4065G0.3435G0.3125G0.3875G0.3825G0.3385G0.3310.375G0.3545G0.3775G0.3315G0.0415G5G0.403

0.0425H0.0410.040.0750.2680.2540.0475H0.265H0.2635H0.2685H0.2685H0.265H0.2650.2895H0.2775H0.2735H0.2795H0.2695H5H0.275

0.0416A0.0390.0380.1290.0380.3170.3136A0.3746A0.3216A0.2946A0.426A0.3226A0.4740.2716A0.4216A0.3816A0.4686A0.0416A6A0.384

0.3596B0.3620.0440.1150.2260.3870.46B0.3896B0.366B0.3636B0.4296B0.4036B0.4090.416B0.3956B0.4126B0.346B0.0416B6B0.35

0.0456C0.0440.0440.1140.0450.2850.2816C0.3216C0.3336C0.3656C0.2836C0.3926C0.3160.3646C0.4166C0.3516C0.4866C0.0446C6C0.351

0.2946D0.2830.0440.1180.290.3240.3266D0.3766D0.4086D0.0466D0.3056D0.256D0.0440.3056D0.336D0.366D0.3746D0.0426D6D0.053

0.3096E0.3880.0440.1140.0440.0460.0486E0.3766E0.4126E0.386E0.2676E0.276E0.3340.3086E0.2496E0.2526E0.3136E0.0436E6E0.471

0.0426F0.0430.0420.5480.0430.0450.2746F0.2886F0.4146F0.3026F0.3296F0.2756F0.4750.3476F0.4266F0.2876F0.2756F0.0416F6F0.334

0.0456G0.0410.0410.0930.0440.2730.2826G0.3146G0.3156G0.366G0.3556G0.3516G0.3190.4556G0.4176G0.3276G0.2866G0.046G6G0.053

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nyanchuk:in random worksheet these samples are highlighted with red

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Selection for high β-glucan content and good agronomic performance in oat grain

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Transcript of Selection for high β-glucan content and good agronomic performance in oat grain

Selection for high β-glucan content and good agronomic performance in oat grain Alona A. Chernyshova Professor: Jean-Luc Jannink Iowa State University.

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