Primary antibody Working dilution Secondary antibody Vendor Catalog # Acetylated α-tubulin 1:2500 Mouse Sigma T-6793 Actin Rabbit Sigma A 5060 α-tubulin 1:500 Mouse Sigma T 5168 Apaf-1 (2E10) 1:50 – 1:500, Mouse Santa Cruz sc-65890 Apaf-1 (H-324) 1:50 – 1:500, Rabbit Santa Cruz sc-8339 Beta-galactosidase 1:100 Chicken GeneTex GTX29361 BrdU Mouse Sigma B 8434 Chx10 Sheep Exalpha Biologicals X1179P Cleaved Caspase-3 1:200 for IF and

IH Rabbit Cell Signaling 9661

CRE 1:500 Mouse Sigma C7988 CYN1 (neurons, cytoplasmic)

1:10 Mouse Developmental Hybridoma Bank CYN-1

Cytochrome C 1:1000 Mouse BD Pharmingen 556433 eIF-4E 1:1500 -1:2000 Mouse BD Transduction Laboratories 610270 Engrail-1 1:10 Mouse Developmental Hybridoma Bank 4G11 FLAG 1:1000 Mouse Stratagene 200471 FOXA2 (Hnf3β) 1:10 Mouse Developmental Hybridoma Bank 4C7 FOXG1 Rabbit abcam ab18259 FP3 (floor plate marker)

1:10 Mouse Developmental Hybridoma Bank FP3

γ-tubulin 1:1000 Mouse Sigma T 6557 GFP (monoclonal) Mouse Chemicon MAB3580 GFAP 1:1000 Rabbit Dako Cytomation Z 0334 GM130:FITC 1:3000 Mouse BD Transduction Laboratories 612008 HA 1:1000 Mouse Roche 11666606001 HB9 1:10 Mouse Developmental Hybridoma Bank 81.5C10 His 1:100 Rabbit Cell Signaling 2365 Ki67 1:500 Rabbit abcam ab15580

LIM2 1:10 Mouse Developmental Hybridoma Bank 4F2 LIM3 1:10 Mouse Developmental Hybridoma Bank 67.4E12 Math1 1:10 Mouse Developmental Hybridoma Bank Math11 MBP (monoclonal) 1:200 Rat Chemicon MAB386 MBP (polyclonal) 1:200 Rabbit Chemicon AB5864 Neurofil 1:10 Mouse Developmental Hybridoma Bank 4H6 or 2H3 Nkx2.2 1:5 Mouse Developmental Hybridoma Bank 74.5A5 Nkx6.1 1:10 mouse Developmental Hybridoma Bank F55A12 OLIG2 1:300 Rabbit Chemicon AB9610 OPA1 1:100 Rabbit abcam ab42364 P75 Promega 23642601 PAX6 1:10 Mouse Developmental Hybridoma Bank PAX6 PDGFRα 1:100 Rat BD Pharmingen 558774 Phospho-Histone H3 1:1000 Rabbit Millipore 06-570 Phospho-Smad1 1:200 Rabbit Cell Signaling 9511 Polycystin-1 1:25 Goat Santa Cruz Sc-10374 Radial glial cell marker 1:10 Mouse Developmental Hybridoma Bank RC2 SMOH 1:200 Rabbit Life Span Bioscience LS-A2666 Smoothened 1:25 Goat Santa Cruze Sc-6366 Sonic Hedghog 1:10 Mouse Developmental Hybridoma Bank Shh Sox10 Rabbit Millipore AB5727 Sufu (suppressor of fused)

1:25 Goat Santa Cruze Sc-10933

Tubulin Mouse Sigma T 5168 V5 1:1000 Rabbit abcam ab9116

Secondary antibody

Working dilution Source and Label Vendor Catalog #

Anti-chicken 1:100 Goat, Alexa Fluor 488 Invitrogen A11039 Chicken, Alexa Fluor 488 Invitrogen A21467 Anti-Goat 1:200 Chicken, Alexa Fluor 568 Invitorgen A11057 Donkey, Alexa Fluor 488 Invitrogen A21202 Goat, Alexa Fluor 350 Invitrogen A11045 Goat, Alexa Fluor 488 Invitrogen A11029

Anti-mouse 1:200

Goat, Alexa Fluor 568 Invitrogen A11031 Anti-mouse 1:2000 for WB Donkey, HRP Jackson ImmunoResearch 715-035-150

Goat, Alexa Fluor 488 Invitrogen A11073 Anti-guinea pig 1:200 Goat, Alexa Fluor 568 Invitrogen A11075

Anti-hamster 1:200 Goat, FITC Invitrogen HA6001 Anti-rabbit 1:200 Cy3 Jackson ImmunoResearch 111-165-144

Donkey, Alexa Fluor 555 Invitrogen A31572 Donkey, Alexa Fluor 594 Invitrogen A21207 Goat, Alexa Fluor 350 Invitrogen A11046 Goat, Alexa Fluor 488 Invitrogen A11034

1:200

Goat, Alexa Fluor 568 Invitrogen A11011 Anti-rabbit 1:5000 Horseradish peroxidase-linked

species-specific whole antibody GE Healthcare NA934

Anti-rat 1:100 Chicken, Alexa Fluor 594 Invitrogen A21471 Hoechst 1:3000 Acros 33258 Phalloidin 488 1:100 Invitrogen A12379