PSMA4 Polyclonal ANTIBODYCatalog Number:11943-2-AP 2 Publications

For Research Use Only

www.ptglab.com

Basic Information Catalog Number:11943-2-AP

Size:150UL , Concentration: 333 μg/ml byBradford method using BSA as thestandard;

Source:Rabbit

Isotype:IgG

Immunogen Catalog Number:AG2542

GenBank Accession Number:BC056249

GeneID (NCBI):5685

Full Name:proteasome (prosome, macropain)subunit, alpha type, 4

Calculated MW:237 aa, 27 kDa

Observed MW:29 kDa

Purification Method:Antigen affinity purification

Recommended Dilutions:WB 1:500-1:2000 IP 0.5-4.0 ug for IP and 1:500-1:1000for WBIHC 1:100-1:400 IF 1:50-1:500

Applications Tested Applications:IF, IHC, IP, WB, ELISA

Cited Applications:IHC, WB

Species Specificity:human, mouse, rat

Cited Species:human, mouse

Note-IHC: suggested antigen retrieval withTE buffer pH 9.0; (*) Alternatively, antigenretrieval may be performed with citratebuffer pH 6.0

Positive Controls:

WB : K-562 cells, BxPC-3 cells, mouse kidney tissue,mouse spleen tissue, U-937 cells

IP : BxPC-3 cells,

IHC : human pancreas cancer tissue,

IF : HeLa cells,

Background Information

Notable Publications Author Pubmed ID Journal Application

Jayashree Chadchankar 31703099 PLoS One WB

Hiroyuki Kondo 31915251 J Biol Chem WB,IHC

Storage Storage:Store at -20°C. Stable for one year after shipment.Storage Buffer:PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Aliquoting is unnecessary for -20ºC storage

T: 1 (888) 4PTGLAB (1-888-478-4522) (toll freein USA), or 1(312) 455-8498 (outside USA)

E: proteintech@ptglab.com W: ptglab.com

For technical support and original validation data for this product please contact: This product is exclusively available under ProteintechGroup brand and is not available to purchase from anyother manufacturer.

Selected Validation Data

K-562 cells were subjected to SDS PAGE followedby western blot with 11943-2-AP (PSMA4 Antibody)at dilution of 1:1000 incubated at 4 degree celsiusover night.

IP Result of anti-PSMA4 (IP:11943-2-AP, 4ug;Detection:11943-2-AP 1:500) with BxPC-3 cellslysate 2800ug.

Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slideusing 11943-2-AP (PSMA4 Antibody) at dilution of1:200 (under 10x lens). Heat mediated antigenretrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slideusing 11943-2-AP (PSMA4 Antibody) at dilution of1:200 (under 40x lens). Heat mediated antigenretrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixedHeLa cells using 11943-2-AP (PSMA4 antibody) atdilution of 1:50 and Alexa Fluor 488-conjugatedAffiniPure Goat Anti-Rabbit IgG(H+L).